Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,895,645	C→T	G322D (GGC→GAC)	adeP ←	adenine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,895,645	0	C	T	95.6%	197.5 / NA	69	G322D (GGC→GAC)	adeP	adenine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (31/34);  minor base G (2/1);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGAACAGATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGCCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCC  >  NC_000913/3895400‑3895890                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      tACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGAACAGATTCAGGAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GGAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGAACGCCAACCGATACGGCGGa                                                                                                                                                                                                                                                   >  2:322991/1‑250 (MQ=255)     ttCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGAACAGATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAgg                                                                                                                                                                                                                                              >  1:394810/1‑251 (MQ=255)      cgggaaatgaagaagtgaaaggccatcacCACGGGGGTAAAAACCGCGGGAACAGAT‑CAATAAAGACCCTGCCGGTTCCCGCGTGCCAGACTGGAAGCCATAGGCACGCCAACGTAAATCAGCGCCCA‑AGCGGCAGC‑GTAACCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGa                                                                                                                                                                                                                                             <  2:233589/228‑1 (MQ=255)                  aaGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGAACAGATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGc                                                                                                                                                                                                                                 >  1:148841/1‑251 (MQ=255)                      cgAACGGCACCATCCCGGCGGTAATAACCGCCGGAACAGATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCAAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTa                                                                                                                                                                                                                             <  1:151052/250‑1 (MQ=255)                           ggCATCATCACGGCGGTAATAAACGCCGGAACAGATTTCGTAA‑GATCCTGCCAGTTCCCGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACCGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAAAGGACTCAATATAAACAGTAACgg                                                                                                                                                                                                                         >  2:228893/1‑250 (MQ=255)                            gCATCATCCCGGCGGTAATAACCGCCGGAACAGTTTCATTAA‑GCTCCTGCCATTTCACGCGTGCCAGACTTGAAGTCCTCAGCCCGCCAACGTAAACCAGCGCCCC‑AGCTGCAGC‑GTACCCTGGCCCCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGgaa                                                                                                                                                                                                                       <  1:132181/251‑1 (MQ=255)                            gCATCATCACGGCGGAAATAAAAGCCGGAAAAGATTCAGTAA‑AATCCTGCCAGTTCACGCGTGCCAGACTGGAAGACATCAGCACGCCCACGTAAATCAGCACACC‑AGCTGCAGC‑GTAGCCCGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCCGCAGGAACAGCAAACCAACAACCACCGCCGTCAGACCGGGACGACCGCCAACCGATACGGCGGAAAAGGGCCCAATATaaacacaaaaggg                                                                                                                                                                                                                        >  2:263441/1‑239 (MQ=255)                                catcaCGGCGGTAATAAACGCCGGAACAGATTTAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCCACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGgaaga                                                                                                                                                                                                                     >  2:120398/1‑249 (MQ=255)                                    aCGGCGGTAATAAACGCCGGAACAAATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGTAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCGATATAAGCCGTAACGGAAGAAGTAcc                                                                                                                                                                                                               <  1:35495/251‑1 (MQ=255)                                        gggTAATAAACGCCGAAAAAGATTCAGTAA‑GATCCTGCCAGTCCACGCGTGCCAGACTGAAATTCATCAGCACCCCACCGTAAACCAGCGCACC‑GGCTGCGGC‑GTAGCCTGCCACCACCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATa                                                                                                                                                                                                           <  2:411410/250‑1 (MQ=255)                                             aaaaccGCCGAAACAGATTCAGGAA‑GACCCTGCCAGTTCACGTTTGCAAGCCTGAAAGTCATCAGAACGCCAGCGTAAATCAGCGCACC‑AACTATGGC‑GTGATCTGCCCCCATCCCCGCCAGCGGCGACAGAAAGATAAGCAGCAGGAACAGCAGACCAACAACCACCGCCGTCACACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACTGAAGAAGTACCGATAAACGa                                                                                                                                                                                                      <  1:417432/246‑1 (MQ=255)                                              taaACGCCGGAACAGATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGa                                                                                                                                                                                                      >  1:317389/1‑250 (MQ=255)                                               aaaCGCCGGAACAGATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACNAAc                                                                                                                                                                                                    >  2:379895/1‑251 (MQ=255)                                                aaCGCCGGAACAGATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAAcc                                                                                                                                                                                                   >  1:67266/1‑251 (MQ=255)                                                    ccGGAACAGATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTc                                                                                                                                                                                               >  2:175799/1‑251 (MQ=255)                                                        aaCAGATCTAGTAA‑GCTCCACCCAGTTCACGCGTGCCAGACTGGAAGCCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GCAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGAAACAGCAGACCAACAACCCCTGCCGTCAAACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAAAAGTACCGATAAACGAACCGGTCACgg                                                                                                                                                                                           >  2:43064/1‑251 (MQ=255)                                                         aCAGATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACgg                                                                                                                                                                                           >  2:309966/1‑250 (MQ=255)                                                         aCAGATTAAGTAA‑GAGCCTGCCAGTTCCCGCGTGCCAGACTGGAAGTCAGCACCACGCCAACGTAAAACAGCCCCCC‑AGCTGCAGC‑GTAGCCTGGCTCCACCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGa                                                                                                                                                                                          <  2:154863/251‑1 (MQ=255)                                                               taaGTAA‑GATCCTGCCAGTTCGCGCGTGCCAGACTGGAAGTCATCAGCACGCAAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAgaga                                                                                                                                                                                     <  2:129895/248‑1 (MQ=255)                                                                cAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAgaga                                                                                                                                                                                     >  1:114986/1‑249 (MQ=255)                                                                cAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGAt                                                                                                                                                                                    >  1:295500/1‑250 (MQ=255)                                                                cAGTAA‑GATCCTGCCAGTAAAAGCGTGCCAGACGGGAAGTCATAAGCACGCCAACGTAAATCAGAGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCAGCCAGCGGCGACAGAACGATAACCAGCAGGAACCGCAGACCAACAACTACTGCAGTCAGACCGGTACGACCACCAACCGATAAGCCGGAAGAGGACGCAATATAAGCCGTAACGAAAGAAGTACAGAAAAAcaacacggtacaggacaagac                                                                                                                                                                                    >  2:220042/1‑230 (MQ=255)                                                                 cataaaaaccccgCCAGTCCACGCTTGCCAGACGGGAATTCACCAGCACCCCAACGTAAAACGGC‑CACCCAGCGGCAGG‑GTGGCCTGGCCCCACCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAgaga                                                                                                                                                                                     <  2:114986/237‑1 (MQ=255)                                                                     a‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTc                                                                                                                                                                              >  2:362046/1‑251 (MQ=255)                                                                                aGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAACCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCACCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAgc                                                                                                                                                                    <  1:345448/251‑1 (MQ=255)                                                                                    cacgcgggcaatgaggaaggcaagcaGCCCGCCAACTTAAACCGGCGCCCC‑AGCGGCAGT‑GTAGCCGGGCCCCACCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCt                                                                                                                                                                <  2:286650/227‑1 (MQ=255)                                                                                     acgcgtgcaaacgtGGAAGTCAGAAGCACGCAAGCGAAAAACACCGCCCC‑AGCTGCAGCAGGA‑CCTGGCACCACCACCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATGAGCCGTAACGGAAGAAGTACCGATAAACGAACCTGTCACGGAAGAGATACGGTGGACATACAGCGCCTg                                                                                                                                                               <  2:53071/238‑1 (MQ=255)                                                                                        cgTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCtt                                                                                                                                                            >  1:110423/1‑251 (MQ=255)                                                                                          tGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTcc                                                                                                                                                          >  1:152267/1‑250 (MQ=255)                                                                                                 cTGGAAGTCATCAGAACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCgg                                                                                                                                                   >  1:98859/1‑251 (MQ=255)                                                                                                            caGCACGCCACCGTAAATCAGCGCACC‑AGCTGCAGC‑GTCGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCt                                                                                                                                        >  2:49362/1‑251 (MQ=255)                                                                                                                     aaCGTAAACCAGCGCACC‑AGCGGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCg                                                                                                                               <  1:120398/251‑1 (MQ=255)                                                                                                                      aagTAATTCAGCGCCCC‑ACCTGCACC‑GTAGCCTGGCCCCACCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAATAAGTACCGATAAACGAACCGGTCACGGAATAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGc                                                                                                                              <  1:5027/249‑1 (MQ=255)                                                                                                                       cgaaaaTCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGcc                                                                                                                             <  1:221175/248‑1 (MQ=255)                                                                                                                       cGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGcc                                                                                                                             <  1:362046/251‑1 (MQ=255)                                                                                                                           aaTCAGCGCCCC‑AGCGGCAGC‑GAAGCCGGGCACCACCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGAc                                                                                                                         <  2:412170/251‑1 (MQ=255)                                                                                                                                  gcccc‑AGCTGCAGC‑GGAGCCGGGCCCCACCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCttt                                                                                                                  <  2:393242/248‑1 (MQ=255)                                                                                                                                          cTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGAAAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACAGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATACGCGGGAATTTCCCATTCTCATCCGCCAGACCTGCTTTATCGGTc                                                                                                           >  1:238203/1‑251 (MQ=255)                                                                                                                                            gCAGC‑GTAGCCGGGCACCACCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCAc                                                                                                         <  1:294383/251‑1 (MQ=255)                                                                                                                                               gC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGTCAGACCTGCTTTATCGGTCACGcc                                                                                                      <  1:323372/251‑1 (MQ=255)                                                                                                                                                  gTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCa                                                                                                     >  2:246792/1‑250 (MQ=255)                                                                                                                                                  gTAGCCTGGAACCATCGCCGCCAGCGACGACAGAAAGATAACCAGCAGGCACAGCAGACCAACAACCACTGCCGTAAGACCGGTACGACCGCCAACCGATACATCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGCGATACTGTCGACATACAGCGCCTGCATCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCAGCTTTATCGCTCACGCCa                                                                                                     >  2:283791/1‑250 (MQ=255)                                                                                                                                                            cccccccccGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAAACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTAcc                                                                                          <  2:148223/246‑1 (MQ=255)                                                                                                                                                                       cAGCGGCGACAGAAAGATAAACAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGtaa                                                                                >  2:194495/1‑248 (MQ=255)                                                                                                                                                                          gggcgACAGAAAGATAACCAGCAGGAACAGCAGACCAAGAACCACTGCCGTCAGACCGGGACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACATCGCCTGCTTCATGCGCGGGAATTTCCCCGTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCaacca                                                                            <  2:338077/250‑4 (MQ=255)                                                                                                                                                                          cggcgACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACa                                                                            <  1:306486/251‑1 (MQ=255)                                                                                                                                                                           ggcgACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACa                                                                            >  2:117101/1‑250 (MQ=255)                                                                                                                                                                            gcgACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGGCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAg                                                                          <  2:361090/251‑1 (MQ=255)                                                                                                                                                                                   aaaGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATCCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCCAACAAGTTGAcc                                                                    >  2:138432/1‑250 (MQ=255)                                                                                                                                                                                           ccAGAAGGAACAGCTGACCAACTACCACTGCCGTCAGACCGGTACGGCCGCCAAGCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTGCCGATAAACGAACCGGTCACGGAATAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAAGCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGaaa                                                           <  2:319430/251‑1 (MQ=255)                                                                                                                                                                                                agGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAgaga                                                       >  2:327301/1‑250 (MQ=255)                                                                                                                                                                                                      agcagACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCAc                                                >  2:188217/1‑251 (MQ=255)                                                                                                                                                                                                         agACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGAAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCGTCTCATCCGCCAGACCTGCTTTAGCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCAcccc                                             <  1:248942/251‑1 (MQ=255)                                                                                                                                                                                                             caacaaCCACTGCCGTCAGCCCGGTACGACCGCCAACCGATACGGCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGcc                                         <  2:110423/251‑1 (MQ=255)                                                                                                                                                                                                              aacaacCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGcc                                         >  1:387842/1‑250 (MQ=255)                                                                                                                                                                                                              aacaacCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCa                                        <  2:148841/251‑1 (MQ=255)                                                                                                                                                                                                               acaacCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAg                                       <  2:67266/251‑1 (MQ=255)                                                                                                                                                                                                                     aCTGCCGTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAg                                 <  1:309966/251‑1 (MQ=255)                                                                                                                                                                                                                           gTCAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAAttt                                                                                                                                             >  1:164382/1‑137 (MQ=255)                                                                                                                                                                                                                             cAGACCGGTACGACCGCCAACCGATACGTCGGAAGAGGACTAAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACg                         <  2:164539/251‑1 (MQ=255)                                                                                                                                                                                                                                    gTACGACCGCCAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGc                  >  2:360213/1‑251 (MQ=255)                                                                                                                                                                                                                                             ccAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTAc         <  1:145577/251‑1 (MQ=255)                                                                                                                                                                                                                                              cAACCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTAc         >  1:377193/1‑250 (MQ=255)                                                                                                                                                                                                                                               aaCCGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACAt       <  1:5465/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGAc    <  1:29440/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cGATACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTAGCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGAc    <  1:307818/251‑1 (MQ=255)                                                                                                                                                                                                                                                    aTACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGAccc  >  1:180281/1‑251 (MQ=255)                                                                                                                                                                                                                                                     tACGTCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGCGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGAccc  >  2:93547/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      TACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGAACAGATTCAGTAA‑GATCCTGCCAGTTCACGCGTGCCAGACTGGAAGTCATCAGCACGCCAACGTAAATCAGCGCACC‑AGCTGCAGC‑GTAGCCTGGCACCATCCCCGCCAGCGGCGACAGAAAGATAACCAGCAGGAACAGCAGACCAACAACCACTGCCGTCAGACCGGTACGACCGCCAACCGATACGCCGGAAGAGGACTCAATATAAGCCGTAACGGAAGAAGTACCGATAAACGAACCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCC  >  NC_000913/3895400‑3895890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑