Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,361,564	C→T	A130V (GCC→GTC)	gltF →	periplasmic protein GltF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,361,564	0	C	T	95.0%	63.1 / ‑6.4	20	A130V (GCC→GTC)	gltF	periplasmic protein GltF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (8/11);  minor base G (1/0);  total (9/11)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

AACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGCCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTG  >  NC_000913/3361348‑3361788                                                                                                                                                                                                                         |                                                                                                                                                                                                                                 aaCATCATAACAGTGCGCTGAATCCAACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACaaaaa                                                                                                                                                                                                <  2:27119/251‑1 (MQ=255)                     aaTCCGACCGGTAAAAGTAATAAACTGGTTCAACTCGGCCGCAAAAACAGCACATTAAATATAACCTGAATAGCACCAACGCTACTTGCTGAAACCTCCAAGGATAACAATCAGCCGACCATTGTAGCGTTCAACGACACATCATATATTGAAAAAGCTTAAGATACTCTCGTAGATATAATGTAAACTAAAAACGTATTTGGTTAAGGTTCTGCGCCTATTGGACAAAAACTTGGCGCTGCAtaaatttt                                                                                                                                                                            >  2:359198/1‑244 (MQ=255)                              ggTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGAc                                                                                                                                                                     >  2:220369/1‑249 (MQ=255)                               gTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCATTGTAGCGTTAAACGACACATACTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAgg                                                                                                                                                                  <  2:15481/251‑1 (MQ=255)                                            aCTGGTACAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCTTTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATTATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGa                                                                                                                                                     <  2:210521/251‑1 (MQ=255)                                               ggTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGATGCTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGTTCTAATGGTGGGAt                                                                                                                                                    <  2:391081/249‑1 (MQ=255)                                                             gCAAAAACAGCACATTAAATATAACCTGCACCGCTCCAACGCTAATTGCTGTAACCTCCAAGGATGAACGTCAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGTAAAAGCTTATGATACTCTCGTAGATATGAAGGGACCGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGAt                                                                                                                                    <  1:304173/251‑1 (MQ=255)                                                               aaaaaCAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATAc                                                                                                                                  <  1:19919/251‑1 (MQ=255)                                                                       cacaTTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGaa                                                                                                                           >  1:84432/1‑250 (MQ=255)                                                                                             gCACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGCTACTCTCGTAGCTATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTAGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTACTGTCGACCTGATTCAAAc                                                                                                    >  1:350886/1‑251 (MQ=255)                                                                                              cACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCATTGTAGCGCTAAACGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGCAAAACGTCTTTGATTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACt                                                                                                   >  1:414043/1‑251 (MQ=255)                                                                                                                              aaCCGTCAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGa                                                                    >  2:156321/1‑250 (MQ=255)                                                                                                                                    cAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTc                                                             <  2:291146/251‑1 (MQ=255)                                                                                                                                        cGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCt                                                         <  1:321630/251‑1 (MQ=255)                                                                                                                                                          aCGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCg                                       <  1:220369/251‑1 (MQ=255)                                                                                                                                                             acacATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTg                                    <  1:251908/251‑1 (MQ=255)                                                                                                                                                                     tataTTGAAAAAGGTGATGATACTATCGTAGATATGAAGGTAACGAAAAACGGCTTTGGTTTAGGTTATGCGCCTAATGGACAAAAACTTGGCCCTGCATCCATTGGTATTGACAGGTCTAATGGAGGGATTCACGCGGCGGACGATACAGGGGAAATTCAAGTCGACCTGATTCAAACTGACCACTGGTCAGAAGAGACCTACACATGGAAAGCCTCATCTACTGGCGCATTCTGCTCGCTGACAAGTTg                            >  2:5761/1‑251 (MQ=255)                                                                                                                                                                                               cGTAGATATGAAGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCTGCAATCGAGCGCGGTTATTCTg  >  2:318945/1‑251 (MQ=255)                                                                                                                                                                                                          aGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGTAAAGCCTCa                                                            <  2:162704/182‑1 (MQ=255)                                                                                                                                                                                                          aGGGAACGAAAAACGTCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCa                                                            >  1:162704/1‑182 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                 AACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAACCTCCAAGGATAACCGTCAGTCGACCATTGTAGCGTTAAACGACACATCCTATATTGAAAAAGCTTATGATACTCTCGTAGATATGAAGGGAACGAAAAACGCCTTTGGTTTAGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTG  >  NC_000913/3361348‑3361788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑