Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,831,373	C→T	A204T (GCG→ACG)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,831,373	0	C	T	97.3%	119.0 / NA	38	A204T (GCG→ACG)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (6/30);  minor base G (0/1);  total (7/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTC  >  NC_000913/1831131‑1831619                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       aCCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTgctg                                                                                                                                                                                                                                                 <  2:355527/250‑1 (MQ=255)  ccGCCATAGGTGGTGCCATGAGTGCCACCGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTgctggc                                                                                                                                                                                                                                               <  2:412433/251‑1 (MQ=255)         aGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGcac                                                                                                                                                                                                                                         <  2:77335/250‑1 (MQ=255)          gggggtgCCATGAGTGCCAACGGTCATCACGCGGGCGCACCCGTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCGCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGcacca                                                                                                                                                                                                                                       <  2:8373/248‑1 (MQ=255)             ggtgCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCacac                                                                                                                                                                                                                                    <  1:317778/251‑1 (MQ=255)                     gAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTc                                                                                                                                                                                                                             <  1:379303/250‑1 (MQ=255)                                                 ctctTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCga                                                                                                                                                                                                  <  2:352061/249‑2 (MQ=255)                                                                               gaacccgccgCCCAGCGCTTTGGCGGTAGCTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCCCGCAGACCTTGTAAAAACGTGTGGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATGACCGCACAGGGAGAGTCGTCAATCAGCGCGCTGGCag                                                                                                                                                                  <  2:264035/247‑1 (MQ=255)                                                                                 aaCCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCagag                                                                                                                                                                <  1:81305/251‑1 (MQ=255)                                                                                              cgcTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTtat                                                                                                                                                   <  1:276974/251‑1 (MQ=255)                                                                                                          aGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCTGCATGa                                                                                                                                        <  1:339328/250‑1 (MQ=255)                                                                                                          aGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAAATCACCCAGACCTTGTAAAAAAAAGTTTCTGGCGGGCGCCACCCCCCCCTCCCCCTCGATGGGGTCGACAATCACCCCACCCGGAGAGTCGGCAATAAGCGCGCTGGCAGGGGTAATATCGTTATATGCAGCATGAc                                                                                                                                       >  1:334159/1‑251 (MQ=255)                                                                                                              aaCAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAat                                                                                                                                   <  1:196986/251‑1 (MQ=255)                                                                                                                     cAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATccgcc                                                                                                                            <  2:115441/251‑1 (MQ=255)                                                                                                                        gCGTCCCGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGGCGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGATTACACAATTCCCGCAGACCTTGTAACAACGTGTTGCTGGCTGGCCCCACACCGCCTTCCCCCTGGTTGGTTTCGACAATCACCGCACAGGTAGAGTCGTCAATAAGCGCGCTTGCAGAGTTAATTTCGTTATATGCAGCATGACGAATATccgccgtc                                                                                                                         <  1:143908/251‑3 (MQ=255)                                                                                                                           ccACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGt                                                                                                                      <  1:392696/250‑1 (MQ=255)                                                                                                                             aCGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGTCGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGGGTTGCTGGCTGTCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGGCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCAGGACGAATATCCGCGGGCAGTgg                                                                                                                    <  2:245090/251‑1 (MQ=255)                                                                                                                                               gCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAACTCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATa                                                                                                  <  2:125915/251‑1 (MQ=255)                                                                                                                                                cATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGGCCTTGTAAAAACGTGTGGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGGCAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATAGCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAg                                                                                                 <  1:405962/251‑1 (MQ=255)                                                                                                                                                 aTACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAgg                                                                                                <  1:320085/251‑1 (MQ=255)                                                                                                                                                   aCAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAAACACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggct                                                                                              <  1:37971/251‑1 (MQ=255)                                                                                                                                                             ggTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCAc                                                                                    <  2:10507/251‑1 (MQ=255)                                                                                                                                                                gcgcCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCg                                                                                 <  1:411977/251‑1 (MQ=255)                                                                                                                                                                        cGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAAACACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACa                                                                         <  1:235286/251‑1 (MQ=255)                                                                                                                                                                                 gTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGc                                                                <  1:91679/251‑1 (MQ=255)                                                                                                                                                                                       catcaAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGa                                                          <  2:289934/251‑1 (MQ=255)                                                                                                                                                                                            aaaaTCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAc                                                         <  2:245479/247‑1 (MQ=255)                                                                                                                                                                                                 cAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCTCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAc                                                 <  1:79865/250‑1 (MQ=255)                                                                                                                                                                                                    cAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAt                                             <  1:211269/251‑1 (MQ=255)                                                                                                                                                                                                                        acaATTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCg                         >  1:276361/1‑251 (MQ=255)                                                                                                                                                                                                                           aTTCACGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGctc                      <  2:221654/251‑1 (MQ=255)                                                                                                                                                                                                                               aCGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                  <  1:376359/251‑1 (MQ=255)                                                                                                                                                                                                                                cGCAGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATg                 >  1:228348/1‑251 (MQ=255)                                                                                                                                                                                                                                   aGACCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGCACGCCACGATGCCGCTCTTATgtct              >  2:277125/1‑248 (MQ=255)                                                                                                                                                                                                                                     aCCTTGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGc            >  1:87238/1‑251 (MQ=255)                                                                                                                                                                                                                                       cTTGTAAAAACGTGTTGCTGTCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCg          >  1:277223/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttGTAAAAACGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCgc         >  2:7825/1‑250 (MQ=255)                                                                                                                                                                                                                                               aaCGTGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGAGGCCGCTCTTATGGCTGCCGTAGCGGTc  <  1:148209/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       ACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTC  >  NC_000913/1831131‑1831619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑