Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,383,369	C→T	K300K (AAG→AAA)	mdh ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,383,369	0	C	T	100.0%	165.2 / NA	47	K300K (AAG→AAA)	mdh	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/22);  total (25/22)

CCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCC  >  NC_000913/3383130‑3383611                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   ccGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCTCCCAGGGCGATATCTTTTTTCAGCGTATc                                                                                                                                                                                                                                         <  2:292704/251‑1 (MQ=255)                                 ggTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATg                                                                                                                                                                                                         <  1:201174/251‑1 (MQ=255)                                          aaTTCGCCCTTGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGTTCACCATTCAGGAATATCCGGTAACTAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAggg                                                                                                                                                                                                <  1:188370/251‑1 (MQ=255)                                                 ccTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCGGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGAt                                                                                                                                                                                         >  2:396041/1‑251 (MQ=255)                                                     gTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGATGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATAAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGAt                                                                                                                                                                                         >  2:94836/1‑247 (MQ=255)                                                        cGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGAttt                                                                                                                                                                                   <  1:79179/250‑1 (MQ=255)                                                              tCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGctct                                                                                                                                                                            >  1:49325/1‑251 (MQ=255)                                                                     agtagtGATGCGAGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACg                                                                                                                                                                      <  2:152075/250‑1 (MQ=255)                                                                           gATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGtttt                                                                                                                                                               <  1:144438/251‑1 (MQ=255)                                                                                 ggatgatAATCAGTTAACGCTGCAGGACACCTGTCAGGAATATCCGGCAACCAATTAATTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCca                                                                                                                                                         <  1:316306/250‑1 (MQ=255)                                                                                  gatgatAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAAt                                                                                                                                                                                                          <  2:83106/201‑1 (MQ=255)                                                                                  gatgatAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAAt                                                                                                                                                                                                          >  1:83106/1‑201 (MQ=255)                                                                                             gTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGATCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGtt                                                                                                                                             >  2:281350/1‑251 (MQ=255)                                                                                              ttaccgccgCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAACCAAAAAACCGGAGTCTGTGCTCCGGATTTTTATTATCCGCTAATCAATTACTTATTAAAGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAACTGCGCTCCGGGTACAGATAGATTTACGCCCTTCCACGCCGTTTTTACCCAGCCGCGGCGGTTg                                                                                                                                            >  2:354463/9‑251 (MQ=255)                                                                                                    cTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAACAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTgagaga                                                                                                                                       >  1:378538/1‑250 (MQ=255)                                                                                                    cTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTgagaga                                                                                                                                       >  1:325828/1‑250 (MQ=255)                                                                                                        aGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGaaa                                                                                                                                  <  1:105838/251‑1 (MQ=255)                                                                                                          gTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACg                                                                                                                                >  1:270694/1‑251 (MQ=255)                                                                                                            cACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACggg                                                                                                                              >  2:3742/1‑251 (MQ=255)                                                                                                                aTTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTa                                                                                                                          <  1:396204/251‑1 (MQ=255)                                                                                                                  tCAGGAATATCCGGCAACCAATTAAGGCGGGCGCTCAAAACAAAAAACCGGCGTCTGTGCTCCGGTGTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCACATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGGTGAGAGAAGAAACGGGCGTACt                                                                                                                        >  2:310331/1‑251 (MQ=255)                                                                                                                               gCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAggg                                                                                                                                                                                                <  1:333203/166‑1 (MQ=255)                                                                                                                               gCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAggg                                                                                                                                                                                                >  2:333203/1‑166 (MQ=255)                                                                                                                                 aaCNAATTAAGTCGTGCGCTCAAAACAAAAAACCGGATTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATTCCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCaa                                                                                                         <  2:378538/251‑1 (MQ=255)                                                                                                                                                     caaaacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACg                                                                                     <  1:358371/251‑1 (MQ=255)                                                                                                                                                      aaaacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCTTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGc                                                                                    <  1:396041/251‑1 (MQ=255)                                                                                                                                                        aacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCCGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGCCCGTCGCCTTCAACGTATGCACATTCGACAACGCCt                                                                                  >  1:59777/1‑251 (MQ=255)                                                                                                                                                                   ggAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCCATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCAAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTg                                                                       >  2:174467/1‑251 (MQ=255)                                                                                                                                                                            gCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCAc                                                               <  1:402717/250‑1 (MQ=255)                                                                                                                                                                            gCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACg                                                              >  1:266760/1‑251 (MQ=255)                                                                                                                                                                               ccGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCTCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGa                                                             <  1:416815/249‑1 (MQ=255)                                                                                                                                                                                     ttttATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCagag                                                      <  1:120377/250‑1 (MQ=255)                                                                                                                                                                                           tatCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGAcc                                               >  2:286722/1‑251 (MQ=255)                                                                                                                                                                                           tatCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATACAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCCCATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGagacagaac                                               >  2:343601/1‑249 (MQ=255)                                                                                                                                                                                              ccGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCaa                                             <  1:46418/250‑1 (MQ=255)                                                                                                                                                                                                         ttACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTGTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCt                                  <  1:170806/250‑1 (MQ=255)                                                                                                                                                                                                             ttattaACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTg                             >  1:198319/1‑251 (MQ=255)                                                                                                                                                                                                                ttaACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGcaca                                                                                               <  2:194396/182‑1 (MQ=255)                                                                                                                                                                                                                ttaACGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGcaca                                                                                               >  1:194396/1‑182 (MQ=255)                                                                                                                                                                                                                   aCGAACTCTTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGATAAGAATCGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCTCCCTGCAGTGCACGAACCAGAGACATACCAAAACGTGCAGCTGCCTGGCCCAt                       <  1:62353/251‑1 (MQ=255)                                                                                                                                                                                                                        ctctTCGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTACGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCAtcgcc                   >  1:158745/1‑246 (MQ=255)                                                                                                                                                                                                                             cGCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGtt             >  2:218127/1‑251 (MQ=255)                                                                                                                                                                                                                              gCCCAGGGCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTg            >  2:193696/1‑251 (MQ=255)                                                                                                                                                                                                                                    ggCGATATCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGAcc      >  1:150960/1‑251 (MQ=255)                                                                                                                                                                                                                                        atatCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGCTAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCCTCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCATCGCATTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGCGCACGCACCAAAGACAGACCACAACGTGCAGCGGCCTGTCCCATCGGCAGGGTTGCAgtccggcc  >  1:9505/1‑244 (MQ=255)                                                                                                                                                                                                                                         tatCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAg                                                                                                    >  2:234449/1‑152 (MQ=255)                                                                                                                                                                                                                                         tatCTTTTTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAg                                                                                                    <  1:234449/152‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   CCGGCCATCTCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACGCCGTTTTTACCCAGCAGCAGCGGTTGAGAGAAGAAACGGGCGTACTGACCGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCC  >  NC_000913/3383130‑3383611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑