Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,845,513	C→T	R483Q (CGG→CAG)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,845,513	0	C	T	100.0%	131.9 / NA	40	R483Q (CGG→CAG)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/24);  total (16/24)

CGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATC  >  NC_000913/1845266‑1845728                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                        cgcCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCATTGCCTCTGTCCCCAGACCATCGGTCGAACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGACAAATGGCGCGGCGGCTGGGTTTgctgct                                                                                                                                                                                                                      >  1:264511/1‑251 (MQ=255)         ccGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTAc                                                                                                                                                                                                                  <  1:129831/247‑1 (MQ=255)         ccGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTAc                                                                                                                                                                                                                  <  1:28263/247‑1 (MQ=255)         ccGGCAGCGAATGGAATACCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCCTGAACACCAGTTCAATACTCCTGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGGGGATCTTTTTCAGATCTTTATCCTGCCCAAACCGCGCGATCCCGGTCATCGCCGGAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCCGGGGTTGCTGctctgcacct                                                                                                                                                                                                              >  2:389245/1‑245 (MQ=255)                cGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCtt                                                                                                                                                                                                       <  1:128065/251‑1 (MQ=255)                          aGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAg                                                                                                                                                                                             <  1:152725/251‑1 (MQ=255)                           gcgcTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGc                                                                                                                                                                                            <  1:186890/251‑1 (MQ=255)                            cgcTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCa                                                                                                                                                                                           <  2:342556/251‑1 (MQ=255)                                               gagtggaTATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCa                                                                                                                                                                                           >  1:33897/1‑232 (MQ=255)                                               gagtggaTATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCa                                                                                                                                                                                           <  2:33897/232‑1 (MQ=255)                                                 gtggaTATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGccaccc                                                                                                                                                                      >  2:346600/1‑250 (MQ=255)                                                       ataGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGCTCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGc                                                                                                                                                                 >  2:193244/1‑250 (MQ=255)                                                       ataGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGAGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCa                                                                                                                                                                <  1:191947/251‑1 (MQ=255)                                                                   tttGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCcgt                                                                                                                                                    >  1:165316/1‑251 (MQ=255)                                                                                ccACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATc                                                                                                                                       <  1:254741/251‑1 (MQ=255)                                                                                 cACGCTTGAACAACAGGTCAATAATCCCGGCACGCCTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATcg                                                                                                                                      <  2:326383/251‑1 (MQ=255)                                                                                       tGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGctct                                                                                                                                >  1:112665/1‑251 (MQ=255)                                                                                                    aataatCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTGTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTTGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACa                                                                                                                   <  1:324397/251‑1 (MQ=255)                                                                                                              gCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCa                                                                                                         <  2:105830/251‑1 (MQ=255)                                                                                                               cACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGGGCGCCac                                                                                                        >  1:210242/1‑250 (MQ=255)                                                                                                                   cgTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTg                                                                                                    <  1:346600/251‑1 (MQ=255)                                                                                                                   cgTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCGTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCGAACAGCGTGCGCCAGCCTg                                                                                                    <  2:6459/251‑1 (MQ=255)                                                                                                                     ttGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGAACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGATGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCt                                                                                                  >  1:187737/1‑251 (MQ=255)                                                                                                                                    gACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcgc                                                                                   <  1:233170/251‑1 (MQ=255)                                                                                                                                       cATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCt                                                                                <  2:112665/251‑1 (MQ=255)                                                                                                                                        aTCGGTCGCACGAAGGATCTTTTTCAGATCTGTATCCTGCACAAAGCGCGCGACCCCGGTCATCGCCGAAGGCAGTGTTGCATCGGTAAAAGGGCGCGGCGGCTGGGGTGGCTGCTCTACCACTTCTCCTTTTTCACACAGCAACTCTTCGCCGTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCGCGTTGCTGCCTAACAGCGTGCGGCATTCTGCTTCAGCAAGAGAACGCGCtt                                                                               <  2:18031/251‑1 (MQ=255)                                                                                                                                          cGGTCGGACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGAGCGAAACAGGTAAACGCCGAAAGCAGTGATGCATCGGTAAAATGGCGCGGAGGCTGGGCTTGCTGCACTACCACTTTACCTTTTTCACACAGCAAATCATCGTCTTTAGTCACAACAGGCAGTGGCGCGGCGTAGTTTTCTTCATCGCGCTCTTTGCTGCATAAAAGCGTGCGGCAGCCTGCTTCAGCAAGAAAACgcgcctt                                                                              >  1:250548/1‑247 (MQ=255)                                                                                                                                               gCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCAGCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCCCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGctac                                                                        <  2:82588/251‑4 (MQ=255)                                                                                                                                                cACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGAc                                                                        >  2:196056/1‑250 (MQ=255)                                                                                                                                                 aCGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACa                                                                       >  1:61213/1‑250 (MQ=255)                                                                                                                                                              ttCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTGCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTgg                                                          <  2:360605/250‑1 (MQ=255)                                                                                                                                                                        tATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGt                                               >  1:119104/1‑251 (MQ=255)                                                                                                                                                                         aTCCTGCACAAAGCGCGCGATAACGGTCATCGCCGAAAGCAGTGCTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTATCGACAAATTTGCCTTTGGCAATGTCCAGtt                                              >  2:274782/1‑251 (MQ=255)                                                                                                                                                                                    aGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAacacac                                   <  1:298552/251‑1 (MQ=255)                                                                                                                                                                                            aTCCCGGTCATCGCCGAAAGCAGTGTTGCATCGTTAAAATGGCGCGGCGGCTGGGTGTGCTGCTCTTCCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCTCCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCTCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCATCAAGaaa                                                                                       <  2:31209/191‑1 (MQ=255)                                                                                                                                                                                            aTCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGAGGCGGCTGGGTTTGCTGCTATACCAATTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGGGCCAGCCCGCTTCAGCAAGaaa                                                                                       >  1:31209/1‑191 (MQ=255)                                                                                                                                                                                                          cGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGc             <  2:165316/251‑1 (MQ=255)                                                                                                                                                                                                                 aGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTg      <  1:260988/251‑1 (MQ=255)                                                                                                                                                                                                                        cATCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATc  >  1:271062/1‑248 (MQ=255)                                                                                                                                                                                                                         aTCGGTAACATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCCTTGCTGCATAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcg                                                                                    >  2:246132/1‑165 (MQ=255)                                                                                                                                                                                                                         aTCGGTAAAATGGCGCGGCGGCTGGGTTTGCTGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTGCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcg                                                                                    <  1:246132/165‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                        CGCCATCTCCGGCAGCGAATGGAATAGCGCTTTTCCGGCGTCGGTGGAGTGGATATAGCGCCCTTTTTTGGTCAGGAAACCACGCTTGAACAACAGTTCAATAATCCCGGCACGCGTTGCCTCTGTCCCCAGACCATCGGTCGCACGGAGGATCTTTTTCAGATCTTTATCCTGCACAAAGCGCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATC  >  NC_000913/1845266‑1845728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑