Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,828,824	C→T	R304Q (CGA→CAA)	astD ←	aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,828,824	0	C	T	100.0%	162.9 / NA	46	R304Q (CGA→CAA)	astD	aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/14);  total (32/14)

TAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATT  >  NC_000913/1828578‑1829068                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     tAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTgctg                                                                                                                                                                                                                                                  >  2:3282/1‑251 (MQ=255)  aaCGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTgctgg                                                                                                                                                                                                                                                 >  1:10006/1‑251 (MQ=255)     gCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTgctggctg                                                                                                                                                                                                                                              >  1:347795/1‑251 (MQ=255)                aaTAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAAcc                                                                                                                                                                                                                                     >  1:364010/1‑249 (MQ=255)                aaTAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATAATCACCGGCGTCAGCAAAGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGAAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGATGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGATGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAAcc                                                                                                                                                                                                                                     >  1:204920/1‑249 (MQ=255)                 aTAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCCACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGACGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCaccc                                                                                                                                                                                                                                     >  1:21932/1‑245 (MQ=255)                    ccggtccaacccccTCTTCATCTGTCACGCCACCACCGCTTGTCTTTTCAATGCCCCCCGGCGTCAGCAACTATGTCCCTGTTTGTAATAACCGCGGCCCAAGCAGGTGTCGTCCCCCCTCCGTTTCCAGTTCCTGCTATGTATTAACCCCCTTCTGTGCGGCCTTTTCAAAAATCAGCCCGCCAATAATCGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGa                                                                                                                                                                                                                               <  1:62745/239‑1 (MQ=255)                     cgttccgacccccttttCATCTGGTCCTCCAGCACCGCCTGTCATTCAAATGACCCCCGGCGCCAGCAACGATGTCCCTGCTTTTAAAAAGCGCGGCGAAGGCAGGGGCCGTCCGCCCATCCCTTCAAGTTCCTGCCATGCATTAACCACCTGCGGTGCGGCCTGTTCAGAAATCATCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAg                                                                                                                                                                                                                              <  1:10012/236‑1 (MQ=255)                     cGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACg                                                                                                                                                                                                                                >  2:9397/1‑249 (MQ=255)                          cGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCa                                                                                                                                                                                                                            >  2:216655/1‑248 (MQ=255)                           gAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGCCGAGCAAGa                                                                                                                                                                                                                         >  2:56209/1‑250 (MQ=255)                              ccccTCTTCATCTGTTACGCCAGCAACGCCTGTCATTTAAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCCCCCATCGCTCCCAGTTGCTGCCATGCAGTAACCACTTGCTGTGCGGCCTGTTCAGAAATCACCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACg                                                                                                                                                                                                                     <  1:273322/249‑1 (MQ=255)                              cacctctccgtcgggaccGCCAGCAACGCCGGTCATTTCAAGAATCCCGGGCGTCAGCAGCGATGCCCCTGCTTGTAAAAAGCGGGGCCCAAGCGGGGGTCGCCCCCCCACCGCTCCCAGTTGCTGCCATGCAGAAACCCCCTGCTGTGCGGCCTGTTAAAAAAACAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACg                                                                                                                                                                                                                     <  2:233651/234‑1 (MQ=255)                                            gTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATAGCCCTGCGcc                                                                                                                                                                                                       >  1:226063/1‑251 (MQ=255)                                                   aGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCttt                                                                                                                                                                                                >  2:118989/1‑251 (MQ=255)                                                         gCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGATTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCGGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCGGAAATCAGAGCGCCAATAAACGGCTGCGGTTCGTCATACCAGTTGCCAGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGAATCGCCCAGCGCCCCGGTTTTCAGcc                                                                                                                                                                                          >  1:143535/1‑250 (MQ=255)                                                          ccTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCa                                                                                                                                                                                          >  2:17519/1‑250 (MQ=255)                                                            tGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTCATTGCTGGCTGACGGCATCCAGACGAGCCAGAAACGCATCGCCCTGCGCCCCGCTTTTCCGCAAt                                                                                                                                                                                        >  2:124626/1‑250 (MQ=255)                                                                   acaatgcccccgggCGCCAGCAACGTTGTCCCTGTTTGAAAGAAGCGCGGCGCAAGCGGGGGCCGTCCCCCCCTCGCTCCCAGTTGCTGCCATCAATTACCCCCCTGCTGGGCGGCTTTTTAAAAAACCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAAcggcg                                                                                                                                                                                <  2:240241/239‑1 (MQ=255)                                                                    cAATGTTCCCCGGCCTCACCAACGATGTCCCTGCTTTTAATAGGCGCGGCGCAGGCAGGGGTCGCCCCCCCACCGCTCCCACTTGCTGCCATGCAGAACCCACCTGCTGTGCGGCCTGTGCAAAAACCACCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAAcggcgg                                                                                                                                                                               <  1:17519/251‑1 (MQ=255)                                                                      aTGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACggcgggc                                                                                                                                                                             >  1:249308/1‑251 (MQ=255)                                                                       tGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACggcgggcg                                                                                                                                                                            >  1:316365/1‑251 (MQ=255)                                                                                            aTGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCt                                                                                                                                                       >  1:242536/1‑251 (MQ=255)                                                                                                       tGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAACAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGc                                                                                                                                            >  1:109139/1‑251 (MQ=255)                                                                                                                cgcgGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGGGCAGCGTTGACCGGCTGAGACAAACGCCGACCGaa                                                                                                                                    >  1:246950/1‑250 (MQ=255)                                                                                                                       cAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTTACCGGCTGTGACAAACGCCGACTGAATGGTc                                                                                                                               >  2:363644/1‑248 (MQ=255)                                                                                                                                   ccccGCCCTTCGTTCCCAGTGGCTGCCACGCATTACCCACCGGCTGTGGGGCTTGTTAAAAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCcgcg                                                                                                                <  2:10006/249‑1 (MQ=255)                                                                                                                                     ccGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAAAGCCGACTGAATGGGCAGATGGACAGCCGCGTc                                                                                                              >  1:391536/1‑251 (MQ=255)                                                                                                                                       gCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGCCTGAATGGTCAGATGGCCAGCcgcggcg                                                                                                             >  2:68320/1‑247 (MQ=255)                                                                                                                                                    aGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTc                                                                                               >  1:330022/1‑251 (MQ=255)                                                                                                                                                        gcccccagaccgaagcccccggctgCGCGGCTTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATc                                                                                            <  1:118989/229‑1 (MQ=255)                                                                                                                                                                     aaCCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGat                                                                              >  1:110910/1‑251 (MQ=255)                                                                                                                                                                         cccTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGattatt                                                                          <  1:216655/250‑1 (MQ=255)                                                                                                                                                                                    ggCCGGTTAAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCAtct                                                               <  1:246557/251‑1 (MQ=255)                                                                                                                                                                                            cAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACCAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCCCCTCATCGATAATTAGCGGATTATTACCGACCATCTCAAGGGCg                                                       >  1:230989/1‑251 (MQ=255)                                                                                                                                                                                                           cAATAACCGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCCAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGTGCGCCGGTGCAGCGTTTACCGGCTTTGCCACACGCCGCCTGCATGGTCTGCTGGACAACCGCGTCGCTATCCGCCCCCTCCTCCATTATTTGCGGTATATTACCGCCCATCTCAAGGGCGCGAATTTtcttctgc                                        >  2:71142/1‑246 (MQ=255)                                                                                                                                                                                                            aaTAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCt                                       <  2:74246/251‑1 (MQ=255)                                                                                                                                                                                                              tAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAACCGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCCGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTgc                                     >  1:85623/1‑250 (MQ=255)                                                                                                                                                                                                              tAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTATCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGccg                                      >  1:17618/1‑248 (MQ=255)                                                                                                                                                                                                                        cGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCt                           <  1:332494/251‑1 (MQ=255)                                                                                                                                                                                                                         ggTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCCACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGGGACAAACACCGACTGAATTGTCAGATGGACAGCCGCGGCGATATCAGCCCCCTCATCGCTAATTAACGGATTATTACCCCCCACCTCAAGGGCCAGAATTTTCTCCCGCTGAACGGagaggtg                          >  2:191997/1‑248 (MQ=255)                                                                                                                                                                                                                            tCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGc                        <  1:28937/250‑1 (MQ=255)                                                                                                                                                                                                                                       gTTGACCGGCGTTAATTGATGGCTGACGGCAGCCAGACGAGCAAGAAACGCATCGACCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGAGGAGCGTTGACCGGATGTGAAAAACGCCGACTGAATGGTCAGATGGACAACCGCGTCAATATCCGCCACCTCATCGATAATTAGAGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGGTGACCGGAGAAGTGGCGATGCAACTGGt            >  1:158368/1‑251 (MQ=255)                                                                                                                                                                                                                                          gCCCGGCGTTAATTGCCGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCATCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAg          <  1:9397/250‑1 (MQ=255)                                                                                                                                                                                                                                              ggCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGCATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCCTCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGt     >  1:380242/1‑251 (MQ=255)                                                                                                                                                                                                                                                 gTTAATTGCTGGCTGACGGCAACCAGACGTGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATCAACGGCGGGCGCATGTGCAGCCTTGACCGTCTTTGACAAACGCCGACTTCATGGTCAGATGGACCGCCCCCTCCATATCCGCCCCCTCATCGCTACTTAACGGCTTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCCGCCGACCGGTAAGCTGGCGATCCAACTGGTACCCTGTAtt  >  2:132548/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     TAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATT  >  NC_000913/1828578‑1829068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑