Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,514,651	G→A	L137L (CTG→TTG)	rpe ←	ribulose‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,514,651	0	G	A	100.0%	211.3 / NA	60	L137L (CTG→TTG)	rpe	ribulose‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/29);  total (31/29)

TCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAGGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATGCCATAGTTAC  >  NC_000913/3514407‑3514890                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                tCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATcac                                                                                                                                                                                                                                           >  2:340796/1‑251 (MQ=255) tCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGACAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCATGGTTGACGGACATCAGCAAGATcac                                                                                                                                                                                                                                           >  1:70421/1‑251 (MQ=255)    cggcgcTTTTCATCAATGTTTTTTTTGTAGTCGGGTTGGTGGAAGTTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATc                                                                                                                                                                                                                                        <  1:280908/249‑1 (MQ=255)      gcgcATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATcc                                                                                                                                                                                                                                       >  1:279734/1‑250 (MQ=255)         cATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCGGTGTTTGAGGAATGAAAGACTGACCGCCGAAAACAGGGCTGACGGACATCAGCAAGATCACATCCAGCt                                                                                                                                                                                                                                   >  2:283444/1‑251 (MQ=255)         cATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCt                                                                                                                                                                                                                                   >  1:315948/1‑251 (MQ=255)            ttCATAAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGACACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTAt                                                                                                                                                                                                                                >  2:391024/1‑251 (MQ=255)              catcaATGACTTTTTTGTAGTCTGGCTGGTAGAAGATTGCCGAACTGGCGACGAACATATCCGAGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTAACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGCCGTACTTCGCGCAGTCTATCCAGAGTTAGAGGAATGACAGACTGACCGTCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATcc                                                                                                                                                                                                                              >  2:46907/1‑251 (MQ=255)                     gtcTTTTTTGTAGTCTGGCGGGGCGAAAATTGCCGAACCGGCGACGAACATACCCTCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACg                                                                                                                                                                                                                        <  1:124897/248‑1 (MQ=255)                      aCTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATAAGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGTACATCAGCAAGATCACATCCAGCTTATCCATCACGt                                                                                                                                                                                                                       >  1:192767/1‑250 (MQ=255)                         tttttGTAGTCGGGCGGGTCGAAGATTGCCGACCCGGGGACGAAAATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTaa                                                                                                                                                                                                                     <  1:74631/249‑1 (MQ=255)                             tgtgtccgggttggtgaaaGATTGCCGAACCGGCGACAAACATACCCGCCCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAg                                                                                                                                                                                                                <  2:97130/233‑1 (MQ=255)                              gTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATcc                                                                                                                                                                                                                  >  1:248726/1‑247 (MQ=255)                                                gATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCCCGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGAGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCg                                                                                                                                                                                            >  1:101950/1‑251 (MQ=255)                                                    gCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGtt                                                                                                                                                                                        >  1:140894/1‑251 (MQ=255)                                                      cGAACGGGCGACGAAAATATCCGCCCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTaa                                                                                                                                                                                      <  1:37276/251‑1 (MQ=255)                                                       gAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTaa                                                                                                                                                                                      >  1:302408/1‑250 (MQ=255)                                                              ggagaaacttaccccgccccgccGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCATTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGa                                                                                                                                                                              <  2:315948/234‑1 (MQ=255)                                                                   aaCATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGc                                                                                                                                                                         >  1:137148/1‑251 (MQ=255)                                                                      atatCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCAAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCttt                                                                                                                                                                      >  1:268675/1‑251 (MQ=255)                                                                        atCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCATCACGCCACCGCCCACTTCTAGTCGAAGGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACGGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCAACACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAGATACCAGACCCGCTTTAc                                                                                                                                                                    >  2:400770/1‑251 (MQ=255)                                                                         ccctcgcccgccGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTGGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACa                                                                                                                                                                   <  1:286381/247‑1 (MQ=255)                                                                             cgcccgccGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGcc                                                                                                                                                                >  2:412481/1‑250 (MQ=255)                                                                               cccgccGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCAtt                                                                                                                                                             >  2:188886/1‑251 (MQ=255)                                                                                 cgccgcAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGGCATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCAtttt                                                                                                                                                           <  1:152519/251‑1 (MQ=255)                                                                                         taaTTTCGCGAATGTTTTTAACCTTCACGCCACCGCCAACTCCTAGTCTAATGTCAAAGCCAGACTCGTCATTACGGCGACGTACTTTGCGCAGTTTACCCAGTTTTTGGGTAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACTTAATCCAGATAGCTCAGAGGTTTCGCCGGGTTAAATACCAGACCCGCGTTACAGCCATTTTCTTTAAt                                                                                                                                                    <  2:388586/248‑1 (MQ=255)                                                                                         cGATTTCGCAAATGTTGTTCCCCTTCACGCCACCGTCCACTTCAAGACGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGGTTGAGGAAGGAAAGACGGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAAGCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTGTAAt                                                                                                                                                    <  2:388975/250‑1 (MQ=255)                                                                                          gATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCCGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTc                                                                                                                                                                                                >  1:200569/1‑205 (MQ=255)                                                                                                  cAATGTTGTTCCCCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGc                                                                                                                                          <  2:217467/251‑1 (MQ=255)                                                                                                      gttgttCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTg                                                                                                                                       <  1:355110/250‑1 (MQ=255)                                                                                                            ccccTTCGCGCCACGGTCCACTTCTAGTCGAATGTCAAACCCAGCCTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAAGACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCTTGCGGTCaa                                                                                                                                <  2:69481/249‑1 (MQ=255)                                                                                                            cACCTTCACGCCGCCGTCCACGTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGTCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCaa                                                                                                                                <  2:176886/251‑1 (MQ=255)                                                                                                            cACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAACCAGACTCGTCGATAAGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAGCCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATGTTCTTTAATCAGTTGCAGCGTGCgtcca                                                                                                                                 >  2:402933/1‑246 (MQ=255)                                                                                                                 tCACGCTACCGTCCACTTATAGTCGAATGTCAAAGCCAGAGTCTTCGATACGGCGACGTCCTTCGCGCAGTTGATCCAGTGTTGAAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCACCACGTAATCCAGATGGCTCAGAGGTGTCGCCGGGTTAAATACCATACCCGCTTTACAGCGATTTTCCCGAATCAGTTGCGGCGTGCGGTCAACAt                                                                                                                             <  2:37158/249‑1 (MQ=255)                                                                                                                      ccACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGa                                                                                                                      <  1:197532/251‑1 (MQ=255)                                                                                                                           gTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCtt                                                                                                                 >  2:107933/1‑251 (MQ=255)                                                                                                                                     aGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGaa                                                                                                        >  1:393753/1‑250 (MQ=255)                                                                                                                                         gAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCATTGTTTGAGGAATTAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGCTGAAAGGt                                                                                                    >  1:171203/1‑250 (MQ=255)                                                                                                                                                 aaGCCAGACTCGTCGCTACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCt                                                                                           >  2:199366/1‑251 (MQ=255)                                                                                                                                                  aGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTg                                                                                          >  1:216908/1‑251 (MQ=255)                                                                                                                                                   gCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTgg                                                                                         <  1:173653/251‑1 (MQ=255)                                                                                                                                                               aaTACGGAGACGTACTTCGCGCAGTTTATCCATTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTTACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCTCCAACGGCAg                                                                             <  2:251775/250‑1 (MQ=255)                                                                                                                                                                  aCGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATGCCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCg                                                                           >  2:259795/1‑250 (MQ=255)                                                                                                                                                                      cGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATc                                                                      >  1:54071/1‑251 (MQ=255)                                                                                                                                                                            aCTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAAGCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAAGCAGGca                                                                 <  2:101950/250‑1 (MQ=255)                                                                                                                                                                                   gcAGTTTCTCCCGTGATTGAGGAGTGAAAGACTGACAGCCGCAACAAGGGTTGACGAACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGCTAGCTCAGAGGTGTCGCCGGGTTAAATACCAGCCCCGCTTTACAGCCATTTTCTTTAACCAGTTTCAGCGCGCGGTCAACATGCTCGGAGGCTTCTGGTTGAAAGGAAATGATGCTGGCACCAGCGGCCGCGAACTCAGGACCAATGCg                                                          >  2:230806/1‑250 (MQ=255)                                                                                                                                                                                     aGTTTATCCAGTTTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGGAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCATAATGCGATc                                                       <  2:54071/251‑1 (MQ=255)                                                                                                                                                                                         tctCCAGTGTTTGAGGAATGAAAGACGGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGTTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCAGTTTCTTTGATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGAGGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGAc                                                    <  1:46907/248‑1 (MQ=255)                                                                                                                                                                                            ccAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACggg                                                 <  1:199366/250‑1 (MQ=255)                                                                                                                                                                                              aGTGTTTGCGGAAAGAAAGCTTTACCGCCGAAGCCATGGTTGACGGACCTCAGCCAGAGCACATCCAGCTTATCCTTCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGTCCCGGTTTACAGCCATCTTCTTTAATCAGTCGCAGCGTGCGGTCAATATGCTCGGAGGCTTCGGAATGAAATATCATTATTCTTCCACCAGCGGCAGCGATATCTGTCACACTGCGATCGTCGGGt                                                <  2:62928/249‑1 (MQ=255)                                                                                                                                                                                                  tttGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCACTTTTCTTTAATCAGTTGCAGCGTGCGGTCACCATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAACTCAGGCACAATGCGATCGACGGGTTTCAcc                                          >  2:385449/1‑251 (MQ=255)                                                                                                                                                                                                  tttGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATAACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCAGCTTTACAGCCATTTTCTTTAATCCGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGACACCAGCGGCAGAGAAATCAGGTACAATGCGATAGACGGGTCTCAcc                                          >  2:323590/1‑251 (MQ=255)                                                                                                                                                                                                    gggggaaacaacgggtgtccgccgAAACCAGGGTTAACGGACATCAGCAAGATCACGGCCAGCTTATCCAGCACGGAATCCAGATAGCTCAGAGGTGGCGCTGGGTTAAATACCAGACCCGCTTTACAGCCATATTCTTTAATAAGGGGGAGCGTGCGGTCAAGATGCGCAGAGACGTCTGGATGAAAAGTAATGATGCTGGGAACAGCGGCAGCGAAATCAAGAACAAAGAGATCGACGGGTTTCTCCAt                                        <  2:138282/233‑1 (MQ=255)                                                                                                                                                                                                     gAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCAt                                        <  1:188886/250‑1 (MQ=255)                                                                                                                                                                                                      aGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAAt                                                                       <  1:205474/218‑1 (MQ=255)                                                                                                                                                                                                      aGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAAt                                                                       >  2:205474/1‑218 (MQ=255)                                                                                                                                                                                                            gAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGgtgt                                 >  1:150313/1‑250 (MQ=255)                                                                                                                                                                                                                    gACCGCCGAAACCAGGTTTGACGGACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTGACAGCCATTTTCTTTAATCAGTTGCAGCGTTCGGTCAACATGCTCGTAGGCTTCTGGATGAAAGGTAATGATGCTTGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGCTTCACCATCAGGTGTACGGCGAt                         <  2:268675/250‑1 (MQ=255)                                                                                                                                                                                                                                        aCGGACATCAGCAAGATCACATCCAGCTTATCCATCCCGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATGCCATAGtt    <  1:283444/251‑1 (MQ=255)                                                                                                                                                                                                                                          ggACATCAGCAAGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATGCCATAGTTAc  <  1:370315/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                TCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGTTTGAGGAATGAAAGACTGACCGCCGAAACCAGGGTTGACGGACATCAGCAGGATCACATCCAGCTTATCCATCACGTAATCCAGATAGCTCAGAGGTGTCGCCGGGTTAAATACCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATGCCATAGTTAC  >  NC_000913/3514407‑3514890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑