Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,432,173	G→A	L98F (CTC→TTC)	smg ←	DUF494 domain‑containing protein Smg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,432,173	0	G	A	100.0%	108.1 / NA	32	L98F (CTC→TTC)	smg	DUF494 domain‑containing protein Smg
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/15);  total (17/15)

TACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTT  >  NC_000913/3431932‑3432418                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      taCGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGaa                                                                                                                                                                                                                                               <  1:375007/250‑1 (MQ=255)       ccGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTc                                                                                                                                                                                                                                        <  1:398506/251‑1 (MQ=255)           gAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAgg                                                                                                                                                                                                                                    >  1:132576/1‑251 (MQ=255)                gTGCTGATTTCGCCATAACAACTTATGATGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTGCCATATGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGaaa                                                                                                                                                                                                                                 >  2:44439/1‑249 (MQ=255)                        ttCGCCATAACAACTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTGTGCCATTTGCTGGGACTCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCTTTATACAGCGCGAGCACTCGCTCTATCTCCATATGACGAGTTTCAAGGTTGAACGCCGGAATCTTCCCAAGGAAAAGTAGAAAc                                                                                                                                                                                                                        <  2:48438/250‑1 (MQ=255)                               aaacaacTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGCACAACCCCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAACCTGCTCAAGGAAAAGCAGAAACCCACGGCa                                                                                                                                                                                                                <  1:98098/250‑1 (MQ=255)                                          gCTGAATTAAAGAAACGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTCCGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGACCTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGAAAAAGCAGAAACCCACGGCAGCTGGCATcc                                                                                                                                                                                                      >  2:343523/1‑250 (MQ=255)                                                  aaaGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCGGGCATCCAGTCGTTca                                                                                                                                                                                             <  2:83360/251‑1 (MQ=255)                                                    aGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCGCGGCAGCTGGCATCCAGTCGTTcaca                                                                                                                                                                                           <  2:139824/251‑1 (MQ=255)                                                              aaTGCAGCATACCTTCATTCACTTCAAAGAGTAATGCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACACATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCATGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGTAGCTGGCATCCAGTCGATAACACTATTCCggg                                                                                                                                                                                 >  2:116518/1‑250 (MQ=255)                                                                tGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGgtgt                                                                                                                                                                               >  1:116753/1‑251 (MQ=255)                                                                          cTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGc                                                                                                                                                                     >  2:104785/1‑251 (MQ=255)                                                                              attcaCTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATgg                                                                                                                                                                 <  1:347406/251‑1 (MQ=255)                                                                                                               gTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTg                                                                                                                                >  2:260641/1‑251 (MQ=255)                                                                                                                  cGCATTTTCGCAGCCCGGAATATTGAACAACCCCATCATGATCCCCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCTCTCTATCACCATTTCACGAGTTTCAAGGTTGAACTCCTGAATCTTCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCCGTCGTTCACACTCTTCCGGTGTATAAATTCGCATGGAGAGAGGTTCCGAGGCCAGTTGCATTGGTTCTGCCa                                                                                                                             <  2:252339/251‑1 (MQ=255)                                                                                                                     aTTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGTAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAAcc                                                                                                                          <  1:384314/251‑1 (MQ=255)                                                                                                                            cAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTg                                                                                                                   >  2:39108/1‑251 (MQ=255)                                                                                                                             aGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTg                                                                                                                   >  1:173631/1‑250 (MQ=255)                                                                                                                                      tatTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGc                                                                                                          >  1:282514/1‑250 (MQ=255)                                                                                                                                          gAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGtt                                                                                                     >  1:202254/1‑251 (MQ=255)                                                                                                                                          gAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGt                                                                                                      <  2:136402/250‑1 (MQ=255)                                                                                                                                                    aTCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGcc                                                                                            >  2:292101/1‑250 (MQ=255)                                                                                                                                                      cAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCAt                                                                                          >  1:408272/1‑250 (MQ=255)                                                                                                                                                            cACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAgg                                                                                    <  2:132576/250‑1 (MQ=255)                                                                                                                                                                      aGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGat                                                                         <  1:282015/251‑1 (MQ=255)                                                                                                                                                                        ctcatcCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAACAACTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGatat                                                                       >  2:67480/2‑251 (MQ=255)                                                                                                                                                                                                             aCTCGCTCTATCACCATTTCACGAGTTTAAAGGTTGAACACCTGAATATGCCCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACATACTTACGGTGAATAAATACGAATGGAGAGAGGATCCGAGGCCAGATGCATCGGTTCTGCCAACCCTTCCTGCTAATCAGCAAGTTTTTCCACACATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACTTGCGTCGGTAAGATCCTGTTc                                  >  1:379333/1‑251 (MQ=255)                                                                                                                                                                                                                    ctATCACCATTTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGtt                             <  2:64310/249‑1 (MQ=255)                                                                                                                                                                                                                        cACCAATTCACGAGTTTCAAGGTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACGCTCTTCCGGTGTATAAATACGCATGTAGAGAGGATCCGAGGCGAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGGCtt                       <  2:392547/251‑1 (MQ=255)                                                                                                                                                                                                                                             gTTGAACACCTGAATCTGCTCAAGGAAAAGCAGAAAACCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGATCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCATGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCtt  >  1:367620/1‑251 (MQ=255)                                                                                                                                                                                                                                              ttGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGa                     <  2:52442/231‑1 (MQ=255)                                                                                                                                                                                                                                              ttGAACACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGa                     >  1:52442/1‑231 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      TACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATCCACACGCAACTCAGCTT  >  NC_000913/3431932‑3432418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑