breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 62,379 Δ25,896 bp [rapA][cra] 25 genes
RA 89,089 C→A intergenic (+57/‑545) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,103 G→C intergenic (+71/‑531) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,143 A→G intergenic (+111/‑491) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,145 G→T intergenic (+113/‑489) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,239 G→A intergenic (+207/‑395) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,241 C→A intergenic (+209/‑393) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,263 A→G intergenic (+231/‑371) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,316 T→C intergenic (+284/‑318) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,321 A→G intergenic (+289/‑313) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,471 T→G intergenic (+439/‑163) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,570 A→C intergenic (+538/‑64) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,699 T→C Y22Y (TAT→TAC mraZ → DNA‑binding transcriptional repressor MraZ
RA 89,754 T→C Y41H (TAT→CAT)  mraZ → DNA‑binding transcriptional repressor MraZ
RA 90,432 A→G E113E (GAA→GAG rsmH → 16S rRNA m(4)C1402 methyltransferase
RA 90,435 T→C R114R (CGT→CGC rsmH → 16S rRNA m(4)C1402 methyltransferase
RA 91,904 G→A E164E (GAG→GAA ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 91,916 C→T Y168Y (TAC→TAT ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 91,922 G→A P170P (CCG→CCA ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 91,949 C→T I179I (ATC→ATT ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,096 A→T A228A (GCA→GCT ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,162 C→T N250N (AAC→AAT ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,327 G→T P305P (CCG→CCT ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,354 A→G V314V (GTA→GTG ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,687 A→G P425P (CCA→CCG ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,810 T→C T466T (ACT→ACC ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,843 A→G P477P (CCA→CCG ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 94,278 G→A A371A (GCG→GCA murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 134,256 G→T intergenic (+44/‑132) acnB → / → yacL AcnB/PF06062 family protein YacL
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
JC JC 288,695 IS150 (+) +3 bp coding (466‑468/759 nt) xynR ← DNA‑binding transcriptional repressor XynR
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
MC JC 339,199 Δ36,219 bp [yahG]mhpE 31 genes
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 553,161 G→A intergenic (‑61/+57) purE ← / ← lpxH N(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
JC JC 789,812 IS2 (–) +5 bp coding (164‑168/1149 nt) galK ← galactokinase
RA 791,688 G→A S123F (TCC→TTC)  galE ← UDP‑glucose 4‑epimerase
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
JC JC 1,093,481 IS2 (+) +5 bp coding (606‑610/1359 nt) dgcT → putative diguanylate cyclase DgcT
RA 1,100,070 G→A C144Y (TGT→TAT)  ycdY → chaperone protein YcdY
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,123,556 C→T E167K (GAA→AAA)  grxB ← reduced glutaredoxin 2
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase
RA 1,196,325 A→G K401K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,331,947 C→T G300G (GGC→GGT topA → DNA topoisomerase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,412,897 G→A L113L (CTC→CTT intR ← putative integrase
RA 1,428,087 C→T E214K (GAG→AAG)  insH5 ← IS5 family transposase and trans‑activator
MC JC 1,588,756 Δ970 bp [ydeS][ydeT] [ydeS], [ydeT]
RA 1,610,076 T→G D202A (GAT→GCT)  uxaB ← tagaturonate reductase
RA 1,610,440 C→T E81K (GAA→AAA)  uxaB ← tagaturonate reductase
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
JC JC 1,651,851 IS2 (–) +5 bp intergenic (+1866/+873) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
MC JC 1,704,350 Δ390 bp blr blr
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
RA 1,738,377 C→T H178H (CAC→CAT purR → DNA‑binding transcriptional repressor PurR
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
JC JC 1,853,844 IS5 (+) +4 bp coding (133‑136/1359 nt) ydjE ← putative transporter YdjE
RA 1,856,968 T→A I268F (ATT→TTT)  ydjI ← L‑glycero‑L‑galacto‑octuluronate‑1‑phosphate aldolase
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,915,297 G→A R80C (CGT→TGT)  proQ ← RNA chaperone ProQ
RA 1,975,224 G→A A34V (GCG→GTG)  cheA ← chemotaxis protein CheA
RA 1,977,914 A→C M95R (ATG→AGG)  flhD ← DNA‑binding transcriptional dual regulator FlhD
RA 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 2,003,276 A→C S111A (TCT→GCT)  fliC ← flagellar filament structural protein
RA 2,003,462 C→A A49S (GCG→TCG)  fliC ← flagellar filament structural protein
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,175,991 T→A K111* (AAG→TAG)  gatZ ← putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
JC 2,238,416 Δ19 bp coding (856‑874/1521 nt) mglA ← D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
JC 2,259,814 +GGTA coding (1597/1692 nt) fruA ← fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,263,441 Δ1 bp coding (55/1131 nt) fruB ← fructose‑specific PTS multiphosphoryl transfer protein FruB
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,503,516 C→A G324W (GGG→TGG)  fryA ← putative PTS multiphosphoryl transfer protein FryA
RA 2,660,391 G→A R381C (CGT→TGT)  iscS ← cysteine desulfurase IscS
RA 2,700,747 G→A L84L (CTC→CTT acpS ← holo‑[acyl‑carrier‑protein] synthase
RA 2,748,396 G→A A145A (GCG→GCA ypjD → cytochrome c assembly family protein
RA 2,756,976 G→A R273Q (CGA→CAA)  intA → integrase IntA
RA 2,780,060 G→A H889H (CAC→CAT ypjA ← adhesin‑like autotransporter YpjA
JC JC 2,787,610 IS5 (+) +4 bp intergenic (‑1773/‑1372) ileY ← / → glaH tRNA‑Ile/glutarate dioxygenase GlaH
RA 2,792,209 G→A V313M (GTG→ATG)  gabD → succinate‑semialdehyde dehydrogenase (NADP(+)) GabD
RA 2,800,435 G→A G97D (GGT→GAT)  ygaM → DUF883 domain‑containing protein YgaM
RA 2,810,925 G→A G52G (GGG→GGA mprA → DNA‑binding transcriptional regulator MprA
RA 2,817,850 G→A noncoding (11/77 nt) argQ ← tRNA‑Arg
RA 2,825,328 G→A S83S (TCC→TCT mltB ← membrane‑bound lytic murein transglycosylase B
RA 2,841,238 G→A M83I (ATG→ATA ascB → 6‑phospho‑beta‑glucosidase AscB
RA 2,863,773 C→T L61F (CTT→TTT)  ygbL → putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL
RA 2,878,077 C→T intergenic (+459/+492) iap → / ← cas2 alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
RA 2,889,365 C→T K245K (AAG→AAA cysI ← sulfite reductase, hemoprotein subunit
RA 2,905,825 C→T Q39* (CAA→TAA)  ygcG → TPM domain‑containing protein YgcG
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 2,926,292 C→T intergenic (+96/‑16) queF → / → ppnN 7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,367,944 G→A A40T (GCG→ACG)  yhcG → DUF1016 domain‑containing protein YhcG
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,399,929 A→T L17Q (CTG→CAG)  mreC ← cell shape determining protein MreC
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,449,779 G→A S35S (AGC→AGT rpsC ← 30S ribosomal subunit protein S3
RA 3,468,768 C→T W362* (TGG→TGA chiA ← endochitinase
RA 3,474,425 T→C K43R (AAA→AGA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,543,877 G→A P237P (CCG→CCA rpnA → recombination‑promoting nuclease RpnA
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,629,921 C→T E228K (GAG→AAG)  yhiI ← putative membrane fusion protein YhiI
JC JC 3,647,663 IS5 (+) +4 bp intergenic (+12/+39) gor → / ← dinQ glutathione reductase (NADPH)/membrane toxin DinQ
RA 3,682,515 C→T M311I (ATG→ATA dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,696,909 G→A G151E (GGA→GAA)  bcsE → c‑di‑GMP‑binding protein BcsE
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,761,230 G→A S7F (TCC→TTC)  selA ← selenocysteine synthase
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,883,678 G→A P18S (CCA→TCA)  dnaA ← chromosomal replication initiator protein DnaA
RA 3,912,074 G→A S532F (TCC→TTC)  glmS ← L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
RA 3,955,304 C→T R609R (CGC→CGT ilvD → dihydroxy‑acid dehydratase
RA 3,988,115 C→T D318N (GAT→AAT)  hemX ← PF04375 family protein HemX
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA
RA 4,011,802 C→T P243S (CCG→TCG)  bioP → biotin transporter
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,210,041 G→A intergenic (+192/‑2) gltV → / → rrlE tRNA‑Glu/23S ribosomal RNA
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,337,390 C→T K180K (AAG→AAA adiY ← DNA‑binding transcriptional activator AdiY
RA 4,338,916 C→T V536I (GTC→ATC)  adiA ← arginine decarboxylase, degradative
RA 4,349,993 C→T A14A (GCG→GCA dcuR ← DNA‑binding transcriptional activator DcuR
RA 4,375,354 G→A P102S (CCT→TCT)  epmB ← lysine 2,3‑aminomutase
RA 4,380,367 C→T D51N (GAC→AAC)  frdB ← fumarate reductase iron‑sulfur protein
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,436,956 2 bp→TT coding (202‑203/741 nt) cysQ → 3'(2'),5'‑bisphosphate nucleotidase
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,578,548 G→A Q264* (CAG→TAG) 
Q264* (CAG→TAG) 
mcrB ←
mcrB ←
5‑methylcytosine‑specific restriction enzyme subunit McrB
5‑methylcytosine‑specific restriction enzyme subunit McrB
RA 4,585,230 A→G L511P (CTG→CCG)  hsdR ← type I restriction enzyme EcoKI endonuclease subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196352 1211419 15068 16 [15] [15] 16 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270–1978505 11–776 58 [0] [1] 51 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3708450 3708545 96 16 [15] [15] 17 dppA/proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
* * ÷ NC_000913 3929340 3938982 9643 103 [1] [2] 75 [viaA]–[rbsR] [viaA],ravA,kup,rbsD,rbsA,rbsC,rbsB,rbsZ,rbsK,[rbsR]
* * ÷ NC_000913 4283247 4289447 6201 65 [4] [5] 125 actP–[nrfB] actP,yjcH,acs,ytcB,nrfA,[nrfB]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994987 (0.090)39 (0.490) 36/482 1.2 85.8% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 6 (0.080)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)58 (0.710) 56/490 0.4 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =1 (0.010)50 (0.620) 47/490 0.7 98.0% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 3583428 =NA (NA)61 (0.750) 57/488 0.4 95.3% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 4283246 3 (0.040)intergenic (‑171/+7) yjcF/actP pentapeptide repeat‑containing protein YjcF/acetate/glycolate:cation symporter
* ? NC_000913 = 3584195NA (NA)119 (1.470) 100/490 0.0 96.8% noncoding (768/768 nt) IS1 repeat region
?NC_000913 4289448 = 4 (0.050)coding (204/567 nt) nrfB periplasmic nitrite reductase penta‑heme c‑type cytochrome
* ? NC_000913 = 39293391 (0.010)102 (1.260) 90/490 0.0 99.0% coding (265/1452 nt) viaA protein ViaA
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 3938983 =2 (0.020)73 (0.900) 60/490 0.3 97.3% coding (757/993 nt) rbsR DNA‑binding transcriptional dual regulator RbsR
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region