Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,850	G→A	noncoding (11/77 nt)	argQ ←	tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,850	0	G	A	95.9%	122.1 / ‑3.7	49	noncoding (11/77 nt)	argQ	tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (17/30);  total (18/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

GAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCC  >  NC_000913/2817603‑2818086                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             gAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGGAGGTTACGGAGCATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                                                                                                                                                                                                            >  2:353017/1‑250 (MQ=255)      aTTTAGAAATTAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACGCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGAt                                                                                                                                                                                                                                      <  2:320466/251‑1 (MQ=255)      aTTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGAt                                                                                                                                                                                                                                      <  1:819633/251‑1 (MQ=255)          aGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCAt                                                                                                                                                                                                                                  <  1:434194/251‑1 (MQ=255)           gAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCAtt                                                                                                                                                                                                                                 <  1:257814/251‑2 (MQ=255)            aaaTGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCg                                                                                                                                                                                                                                <  1:521400/251‑1 (MQ=255)                gggaaagttttGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTTGCCCTTCGGGCAGTGGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAAGACCGCTATCTCTTCGATTCCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTGCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGaa                                                                                                                                                                                                                            <  2:49323/244‑1 (MQ=21)                       ttttGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTAt                                                                                                                                                                                                                     >  1:730236/1‑251 (MQ=255)                           gAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTCTCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTCTCCAGCTGAACTACGGATGCATAGGGAAAACTTATTCt                                                                                                                                                                                                                  >  2:394515/1‑250 (MQ=255)                               gAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTActcc                                                                                                                                                                                                             >  1:342326/1‑249 (MQ=255)                               gAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGACTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAAATTATTCTActgc                                                                                                                                                                                                             >  1:662763/1‑251 (MQ=255)                                aaaTTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTActgct                                                                                                                                                                                                            <  1:404483/251‑1 (MQ=255)                                      attGGAGAAGAGGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCGTGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGAGTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGTACTACGGATGCATCGGGAAAACTTATTCTACTGCTGatat                                                                                                                                                                                                       <  2:71884/249‑1 (MQ=21)                                          gagaAGATGGTGCTTCCGGGAGGATGATTCACTGACGTTCGCCCTTCGGGCCGTTTCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGTTTCTTAGCCGATTACTCTATCCAGCTGAACTACGGATGCACCGGGAAAACTTATTCTACTGCTGATATTGATa                                                                                                                                                                                                  <  1:491824/251‑1 (MQ=21)                                          gagaAGAAGGTTCATCCGGGAGGATGAGTCACTGACGTTCGCCCTTCGGGCCGTTGCTTGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCGTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTGCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACAGCTGATATTGATa                                                                                                                                                                                                  <  2:596376/251‑1 (MQ=18)                                             aagaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCg                                                                                                                                                                                               >  1:490108/1‑251 (MQ=255)                                             aagaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCg                                                                                                                                                                                               <  1:514672/251‑1 (MQ=255)                                                 tGGTGCATCCGGGAGGATGACTCCCTGACGTTCGCCCTTCGGGCCGTTGCTGGAGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGTTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTTCTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGcta                                                                                                                                                                                            <  1:820763/250‑1 (MQ=255)                                                         ccGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGTTATTGATACCGCTACTAAAGcc                                                                                                                                                                                    >  1:710616/1‑250 (MQ=255)                                                           ggggggaTCACTCCCTTACGTTCGCCCTTCGGGCCGTTGCTGACGCAACGTTATCCTCCCTGGTGCCTGCGGTTAATTCCGCTATCTCTTCGATTCCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑TTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCatat                                                                                                                                                                                <  2:294721/247‑1 (MQ=11)                                                                  ggcctcacgtactttCGCCCTTCGGGCCGTTGCTTGCGCATCGGTATCCTTCCTGGTTCCGGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGATCCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATTCCGCTACTAAAGCCATATCAATTa                                                                                                                                                                          <  2:1232/238‑1 (MQ=17)                                                                                tCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCAt                                                                                                                                                             <  1:320492/250‑1 (MQ=255)                                                                                    cctgcgggcggttgtgggCGCAACGTTATCCTTCCGGGTGCCTGCGTTTAATACCGCTATCACATAGATACCTTCAGTGATGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCAGCGGGAAAACTTAGTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCggg                                                                                                                                                        <  2:238394/235‑1 (MQ=17)                                                                                                   tggagcAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAATCACGGGTGCATCGGGAAGGGTTAGTCTGTTGCTGATAGTGGTCACGGTACTAAAAACAAATAAATTAAGAGATGAAGAATACGGGAGGATTAcgcaccat                                                                                                                                         >  2:290661/5‑244 (MQ=9)                                                                                                          aCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTc                                                                                                                                  <  1:297361/251‑1 (MQ=255)                                                                                                               aTCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCt                                                                                                                             >  1:763392/1‑251 (MQ=255)                                                                                                                      ctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGACCTTCGGGcc                                                                                                                      >  1:540857/1‑251 (MQ=255)                                                                                                                            ccTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGCACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGTGATGGTGCATCCGGGAGAATTACTCCCCTTGCGGCTCGCCCTGCgggaagttgat                                                                                                                >  1:419050/1‑243 (MQ=17)                                                                                                                                       tACCGCTATCTCTTCGATACCTTCATTGTTGAAGGCTACGGAGAATATAGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCAACTGCTGATATTGTTACCGCCACTAAAGCCCTATCAATTAAGAGATGGTGCATCCGGGAGGGTTAATAGCCTTGCGGCTCAcccctttggccggtgctcaaccaccgtt                                                                                                     >  1:337947/1‑226 (MQ=14)                                                                                                                                                  cgccGATACCTTCATTGGTGAAGGTTACGGAGAATAGGGTG‑CATCCGGGAGGATTCGAACCTACGACCG‑CTCGGTACGTAGCCGAGTACTCTATCCAGCTGAACGACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATGCCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATccc                                                                                             <  2:81498/245‑1 (MQ=18)                                                                                                                                                      gATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGGGCATCCGGGAGGATTACTCGCCTTGCGGCTCGGACATCGGGCCGGTGCTATAACAACGTTAGCATCCCTTGTGcc                                                                                      >  1:869654/1‑251 (MQ=17)                                                                                                                                                             tcatgtcTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCCCCGACCG‑CTCGGTTCGTAGCCGAGTAATCTATCCAGCTGAACTACGTATGCATCGGGAAAACTTATTCTACTGATGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                                                                                <  2:845222/244‑1 (MQ=18)                                                                                                                                                              cATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGtt                                                                               >  1:294883/1‑250 (MQ=255)                                                                                                                                                                     gAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCGCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTcc                                                                         <  1:394515/249‑1 (MQ=255)                                                                                                                                                                         gTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAca                                                                   <  1:174488/251‑2 (MQ=255)                                                                                                                                                                           tccGGAGAATATGGTG‑CAGCCGGGAGGATTCGAACCTCCGACCG‑CTCGTTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACct                                                                  <  2:763392/248‑1 (MQ=255)                                                                                                                                                                            aCGGAGAATA‑GGTGGCATCCGGGAGGAGTTGAAGCTCCGACCGTCCTGGTT‑GATGCCGAGGACTATATCTAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGATTATATTGATACAGCTACTTAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACctct                                                                <  2:676333/251‑1 (MQ=17)                                                                                                                                                                                     atGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAcc                                                        <  1:644577/250‑1 (MQ=255)                                                                                                                                                                                         tG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTCAAGCAACCTTCTCCTCCCTACTGCCTCCGGTTAATTCCCCTACCTCTTCgttcacttcct                                                   >  1:535794/1‑241 (MQ=17)                                                                                                                                                                                               ggggggagatatgaaacTCCGAACG‑ATCGGTGCGTAGGTGGGTACTCTAGCAAGGTGAACTACGGATGTAACGGGAAAACTTAATCGAGTGATGATAATTAGACCGTTTCTTAAGCCATATCCATTAAGAGATGGGGCATCCCGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTg                                              <  2:254473/235‑1 (MQ=9)                                                                                                                                                                                                    aggaTTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAt                                         <  2:294883/251‑1 (MQ=255)                                                                                                                                                                                                         tCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTCCTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCCCCCTTCGGGCCGATGCTCAAACCACGTTATCTTCCCAAGAGCCTGCGGTTAATTTCGCTACCTCTTCGTTACCTTCATTGCTGAAAATTTCgg                                    >  1:701760/1‑251 (MQ=18)                                                                                                                                                                                                           ggaccctcctactgcttgattagTAGCCGAGTAATCACTCCAGGTGAACTACGGATGTATCGGGAAAATTTATTATACTGATGATATAGATACCTCTACTTAAGCCATATTAATTAAGGGATGGTGCATCCGGGAGGATTACTCGCCTTGCTGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                   <  2:464772/229‑1 (MQ=18)                                                                                                                                                                                                                 ccGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat                             <  1:494804/250‑1 (MQ=255)                                                                                                                                                                                                                 ccGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGATTTCATTCCTCGTGCCTGCGGTTAATTTCGCTACCTCTTCGATACCTTCATTTCTGAAGATTTCGGGGAATATg                            >  1:353869/1‑251 (MQ=18)                                                                                                                                                                                                                                   aGCCGAGTTCTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTTCTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAt            <  1:245629/250‑1 (MQ=255)                                                                                                                                                                                                                                    gCCGAGTACTCTATCCAGCTGAACTACGGAGGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc          <  1:319530/251‑1 (MQ=255)                                                                                                                                                                                                                                       gAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGATGCTAAAGCAACGTTATCCTCCCTAGTGCCCGCGGTTATTTCCTCTAACTCTTCGTTACCTTCATTGCTGAACATTTCGGAGAATTTGGGGCATCCGGGAGGATTCGa        >  1:257964/1‑250 (MQ=18)                                                                                                                                                                                                                                            cactaaccAGCAGAACTACGGATGCAACGGAAAAACAGATTCTAATGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGGGCATCAGGGAGGATTACACGCCGTGCGGCTCGCCCGTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAATCGAACCTcc  <  1:129389/245‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             GAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCG‑CTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCC  >  NC_000913/2817603‑2818086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑