Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,331,947	C→T	G300G (GGC→GGT)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,331,947	0	C	T	97.7%	265.2 / ‑2.2	89	G300G (GGC→GGT)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (34/52);  total (34/55)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

AGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTCCTTCTGACGTCAATGTGATG  >  NC_000913/1331700‑1332175                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     agTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGtgtg                                                                                                                                                                                                                                   <  1:734360/251‑1 (MQ=255)   ttCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGACGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGAGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCAAGCACCCGTCTTGGATTTGGTGTGa                                                                                                                                                                                                                                  >  2:697623/1‑250 (MQ=255)        ggAAGTCGATGCCAGCACGACCACGCAATCTGGTGAAGCCTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGCTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGGACCCGTCTTGGATTTGGTGTGaaaaac                                                                                                                                                                                                                             >  1:306453/1‑249 (MQ=255)            gTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCat                                                                                                                                                                                                                        <  1:643169/251‑1 (MQ=255)                     aGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGgc                                                                                                                                                                                                               >  2:611379/1‑251 (MQ=255)                             cacgccgccTGGTGAAGCCGTGGCGTTACTGGTGACTCATCAGAACGTCAAAACGTTCCGTCCGGTCAACAAAGAACAAACGCAGGCTGCGGGAAGTTTGCTGGAAAAATCGCGCTACAGCGTGCTGGAACGGGAAGACAACCCGACAACCTGTAAACCTGGCGCTTCTTTGATTTCCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGtt                                                                                                                                                                                                       <  2:659009/243‑1 (MQ=255)                             cACGCCATCTGGTGAAGCGTTGGCGGTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAATAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCATGATGATGGCGCAGCGtt                                                                                                                                                                                                       <  2:337717/251‑1 (MQ=255)                              aCGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGttt                                                                                                                                                                                                      <  1:399296/251‑1 (MQ=255)                                 ccttCTGGTGAAGCGTTGGCGTTACAGGGGACTCATCATAACGACAAACGGTTCCGAACGGGCAACAAAGAACAAACCCAGGATGTGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTTAAGACAAACCGACAACCAGTAAACCTGGCGCTCTTTTTATTACCTTGACGCTGCAACAAGCTGCCAGCACCCGTCTGGGATGTGGTGTGAAAAAAACCATTATGATGTCGCAGCGTTTGTa                                                                                                                                                                                                   <  2:775696/248‑1 (MQ=255)                                       ggTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATg                                                                                                                                                                                                 <  1:481786/247‑1 (MQ=255)                                       ggTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGGTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGa                                                                                                                                                                                                <  2:248438/248‑1 (MQ=255)                                         tGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGc                                                                                                                                                                                             >  2:297352/1‑249 (MQ=255)                                              cGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAATCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGACTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCtat                                                                                                                                                                                      >  1:425180/1‑251 (MQ=255)                                                    cGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGATGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGTTGATGGCGCAGCGTTTGTATGAAGCAGGCGAAATCACt                                                                                                                                                                                >  2:728185/1‑251 (MQ=255)                                                        acagtcgACTCAACAGAACGACAAACCGTTCCGGCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACCAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGTTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTTTATCACTTACa                                                                                                                                                                            <  2:154488/245‑1 (MQ=255)                                                        aCAGGTGACTCATCAGAACGAAAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTAc                                                                                                                                                                             <  2:628056/250‑1 (MQ=255)                                                         cAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACAt                                                                                                                                                                           <  1:120414/251‑1 (MQ=255)                                                          aGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAGGCAGGCTATATCACTTACATg                                                                                                                                                                          >  1:724033/1‑251 (MQ=255)                                                           ggTGACTCACAAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGGCTGCTGGAAAAAGCGCGCTACAGCGTGCTTGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACTCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCTCTTACATGc                                                                                                                                                                         <  2:803336/251‑1 (MQ=255)                                                                  catcaGAACGACAAACCGTTCCGTCAGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCg                                                                                                                                                                   >  1:630103/1‑250 (MQ=255)                                                                       gAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCCCCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTAAATGCGTACCGACTcc                                                                                                                                                              >  2:450843/1‑250 (MQ=255)                                                                       gAACGACAAACCGTTCCGGCCGGTCAACAAAGAACAAACGCAGGCTGCGGTAAGGCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGAGGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGGACCGACTCCa                                                                                                                                                             <  2:785835/251‑1 (MQ=255)                                                                         aCGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTACGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATACTGGCGCAGCGTTTGTATGAAGCAGGCTAAATCACTTACCTGCGTACCGACTCCACt                                                                                                                                                           >  2:130277/1‑251 (MQ=255)                                                                            gcgaacctgttcgtgcgGTTAACAAAGAACAAACCCAGGCTGTGGTAAGTCTGCTGGAAAAAGCGTGCTACAGCTCGCTGGAACGTGAAGACAAACCGACAACCAGTAACCCTGGCTCGCCGTTTATTACCGCTACTCTGCAACAAGCTGCCAGCGCCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTTTATCACTTACATGCGTACCGAGCCCACTAAc                                                                                                                                                        <  2:724476/236‑1 (MQ=255)                                                                            gcAAACCGTTCCGTCCGGCCAACAAAAAACAAACTCAGGCTGCGGTAAGTATGCTGGAAAAAGCGCGCTACAGCGTGCTGGGACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTAAGCTACAACAAGCTGACAGCACCCGTCTTGGATTTGGTGTGAAAAAGACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAAc                                                                                                                                                        >  1:315283/2‑251 (MQ=255)                                                                            aCAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAATACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAAc                                                                                                                                                        <  2:475431/251‑1 (MQ=255)                                                                                   gTTCCGTCCGGTCAACAAAGAACAAACCCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAACCCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTc                                                                                                                                                 <  2:859958/251‑1 (MQ=255)                                                                                     tccgtccgGTCACCAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTc                                                                                                                                                 >  1:609380/1‑249 (MQ=255)                                                                                     tccgtccgGTCAACAAAGAACACACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACCAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGtc                                                                                                                                                                      >  2:93726/1‑227 (MQ=255)                                                                                     tccgtccgGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTa                                                                                                                                                                      <  1:93726/228‑1 (MQ=255)                                                                                     tccggccgGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAATCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCa                                                                                                                                                <  2:487449/250‑1 (MQ=255)                                                                                        gtccgGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGAc                                                                                                                                            <  1:291860/251‑1 (MQ=255)                                                                                         tccgGTCAACAAAGAACAAACTCAGGCTGCGGAAAGTCTGCTGGAACAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACAAGTAAACCTGGCGCTCCTGTTATTACCTCTACGCTGCAAAAAGCTGCCAGCACCCGTCTTGGATATGGTGTGAAAAAAACCATGATGATGGCGCAACGTTTGTATGAAGAAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGGATCAGGAcg                                                                                                                                           >  2:711906/1‑251 (MQ=255)                                                                                          acgGTCAACAAAGAACAAACTCAGGCTGCGGTAAGGCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGGCTTGGATTTGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGAcg                                                                                                                                           <  2:306595/249‑1 (MQ=255)                                                                                               caacaaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTa                                                                                                                                      <  2:90266/250‑1 (MQ=255)                                                                                                  caaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAat                                                                                                                                   >  1:798732/1‑250 (MQ=255)                                                                                                    aaGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATg                                                                                                                                <  1:277681/251‑1 (MQ=255)                                                                                                      gAACAAACTCCGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGACCGAGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTTGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGt                                                                                                                              <  2:272470/251‑1 (MQ=255)                                                                                                      gAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGt                                                                                                                              >  1:798126/1‑251 (MQ=255)                                                                                                         cAAACTCAGGCCGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCGATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTcg                                                                                                                           <  2:828546/251‑1 (MQ=255)                                                                                                               cAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTTAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTcgcgttt                                                                                                                      <  2:630881/250‑4 (MQ=255)                                                                                                               cAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGCAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAAc                                                                                                                                                        >  2:788240/1‑216 (MQ=255)                                                                                                               cAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCTCTTACATGCGTACCGACTCCACTAAc                                                                                                                                                        <  1:788240/216‑1 (MQ=255)                                                                                                               cAGGCTGCCGTAAGTCGGCCGGAAAAAGCGCGCTACTGCGTGCTGGAACTTGAAGACAAACCGACAACCAGTCAACCTGGCGCTCCTTTTATTTCCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGAGTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTta                                                                                                                     <  2:211390/251‑1 (MQ=255)                                                                                                                aGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGa                                                                                                                                                                  <  2:144343/205‑1 (MQ=255)                                                                                                                aGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGa                                                                                                                                                                  >  1:144343/1‑205 (MQ=255)                                                                                                                     gCGGTAATTCGGCTGGAAAAAGCGCGCTCCAGCGTGCTGGAACGTGAAGACAAACCGCCAACCAGTAAACCTGGCCCTCTTTTTATTACCCCTACGCTGCAACAAGTTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAg                                                                                                               <  1:637854/251‑1 (MQ=255)                                                                                                                     gCGGGAAGTCTGCTGGAAAAAGCGCGCGACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCGCCTTTTATTCCCGCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTTTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAg                                                                                                               <  2:458381/251‑1 (MQ=255)                                                                                                                      cGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGc                                                                                                              <  1:304155/251‑1 (MQ=255)                                                                                                                          aatcttggttgAAAAAGCTAGCTAAAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAAACGGTCGCTCCTTTTATTCCCTCTACGCTGCAAAAAGCGGCCAGCCCCCGTCTTGGATTTGGGGTGAAAAAACCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCTGTTATATCCGCGATa                                                                                                          <  2:82199/241‑1 (MQ=255)                                                                                                                          aaGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATa                                                                                                          <  1:297352/251‑1 (MQ=255)                                                                                                                                gctgGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAATCAGTAAACCTGGCGCGCCTTTTATTACCTCTTCGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTTAAAAAATCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAAtt                                                                                                       <  2:306522/248‑1 (MQ=255)                                                                                                                                 ctgGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAg                                                                                                               <  2:830565/239‑1 (MQ=255)                                                                                                                                 ctgGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAg                                                                                                               >  1:830565/1‑239 (MQ=255)                                                                                                                                   ggAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTa                                                                                                 <  1:661080/251‑1 (MQ=255)                                                                                                                                    gAAATAGCGCGCTACAGCCTGCTTGAAAGTGAAAACAAACCGACAACCAGTAAACCCGGCTGTCCTTTTATTACCTCTACGCTTCAACAAGCTGCCAGCACCCGTCTTTGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGTCTCCACTAACCTGAGTCAGTACGTGGTAAATATGGTTCGCGGTTATATCAGCTATAATTTTGGTaa                                                                                                <  2:588268/251‑1 (MQ=255)                                                                                                                                    gAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTaa                                                                                                <  1:611379/251‑1 (MQ=255)                                                                                                                                      aaaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAAACATGAAGATGGCGCAGCGTTTGTATGACGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAACTATGGTTCGCGGTTATATCAGCGATAATTTTGGTa                                                                                                 >  1:783341/1‑248 (MQ=255)                                                                                                                                       aaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGaa                                                                                             <  1:561475/251‑1 (MQ=255)                                                                                                                                                 aCAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGcc                                                                                    <  1:277627/250‑1 (MQ=255)                                                                                                                                                 aCAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGc                                                                                     <  1:556605/249‑1 (MQ=255)                                                                                                                                                  cAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCgg                                                                                  >  1:175927/1‑251 (MQ=255)                                                                                                                                                  cAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCgg                                                                                  >  1:80270/1‑251 (MQ=255)                                                                                                                                                         cTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGAAGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTcc                                                                           >  1:344262/1‑251 (MQ=255)                                                                                                                                                          tggtcagTGACGACAAACCGACAACCAGGAAACCTGTCGCTCCTTTTATTGCCGCTTCGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTAGGATGCAGGCTAGTTCACTTACATGCGTACCGGCTCCACTAACCTGAGTCAGTACGCGGTAAATATGGTTCGCGGTGATATCAGCGATAAGTTTGGTAAGAAATATCTGCCGGAAAggcg                                                                           <  2:525624/244‑4 (MQ=255)                                                                                                                                                           ggAACGTGAAGCCAAACCGACAACCATTAAACCTGGCGCTCCTTTTATTTCCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGa                                                                         <  2:618899/251‑1 (MQ=255)                                                                                                                                                              actggAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGAGGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATGTTGGTAAGAAATATCTGCCTGAAAGTCCGAATc                                                                      <  2:609380/247‑1 (MQ=255)                                                                                                                                                               cGTGAATACAAACCGACAACCAGTAAACCTGGCGCTCCGTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGGGAAAAAAACCATGATGAGGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCa                                                                     <  2:762541/251‑1 (MQ=255)                                                                                                                                                                  gAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTCCGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGt                                                                   <  1:231157/250‑1 (MQ=255)                                                                                                                                                                  gAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACCTGCGTACCGAATCCACTAACCTGCGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAACTATCTGCCGGAAAGTCCGAATCAGt                                                                   >  2:874802/1‑250 (MQ=255)                                                                                                                                                                        aaaCCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAg                                                            <  2:311260/251‑1 (MQ=255)                                                                                                                                                                        aaaCCGACAACCAGTAAACCTGGCGCTACTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCCCTAACCTGAGTCAGGACGCGGTAACTATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGcc                                                              >  2:734265/1‑249 (MQ=255)                                                                                                                                                                          aCCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCa                                                          <  1:580692/251‑1 (MQ=255)                                                                                                                                                                            cGACAACCAGTAAACCTGGAGCTCCTTTTATTACCTCTACGCTGCAACAAGCTTCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAATCAGGCTAAATCACTTACATGCGTACCACCTCCACTAACCTGAGTCAGGCCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGCAAGAAATATCTGCCGGCAAGTCCGAATCAGtccacctgcaa                                                         >  2:763364/1‑240 (MQ=255)                                                                                                                                                                                    aGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTc                                                <  1:467248/251‑1 (MQ=255)                                                                                                                                                                                     gTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTca                                               <  1:9609/251‑1 (MQ=255)                                                                                                                                                                                      tAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTcac                                              >  1:668250/1‑251 (MQ=255)                                                                                                                                                                                            tGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGACAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGa                                          >  2:270209/1‑249 (MQ=255)                                                                                                                                                                                             ggCGCTCCTTTTATTCCCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTTGAGTGGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACAGGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAAGATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGTAAACTCACAGGAAgc                                       <  2:528569/251‑1 (MQ=255)                                                                                                                                                                                             ggCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGaa                                         <  1:572996/249‑1 (MQ=255)                                                                                                                                                                                                         attaCCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGa                            <  1:501258/250‑1 (MQ=255)                                                                                                                                                                                                           taCCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCAAGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGAtt                          <  2:798126/250‑1 (MQ=255)                                                                                                                                                                                                            aCCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCg                        >  1:592612/1‑251 (MQ=255)                                                                                                                                                                                                               tctACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTcc                     >  1:355739/1‑251 (MQ=255)                                                                                                                                                                                                                 tACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTCCtt                   >  2:835420/1‑251 (MQ=255)                                                                                                                                                                                                                    gCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTCCTTCTg                >  1:428638/1‑251 (MQ=255)                                                                                                                                                                                                                            aaGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTCCTTCTGACGTCAAt        <  1:427974/251‑1 (MQ=255)                                                                                                                                                                                                                            aaGCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTCCTTCTGACGTCAAt        >  1:489782/1‑251 (MQ=255)                                                                                                                                                                                                                              gCTGCCAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTCCTTCTGACGTCAAtgt      <  1:450843/251‑1 (MQ=255)                                                                                                                                                                                                                                  ccAGCACCCGTCTTGGATTTGGTGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCTATTCGTCCTTCTGACGTCAATGTGATg  >  1:355379/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     AGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTCCTTCTGACGTCAATGTGATG  >  NC_000913/1331700‑1332175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑