| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,263,441 | Δ1 bp | coding (55/1131 nt) | fruB ← | fructose‑specific PTS multiphosphoryl transfer protein FruB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,263,441 | 0 | C | . | 98.5% | 293.2 / ‑0.3 | 66 | coding (55/1131 nt) | fruB | fructose‑specific PTS multiphosphoryl transfer protein FruB |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (28/36); total (28/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.73e-01 | |||||||||||
CAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTT > NC_000913/2263195‑2263684 | cagcaATACCGATTGCCACGTACGCTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGATCGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGt < 1:416872/251‑1 (MQ=255) aTACCGATTGCCACGTACGCTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCg > 1:487356/1‑251 (MQ=255) gATTGCCACGTACGCTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCtt < 1:287102/251‑1 (MQ=255) gCCACGTACGCTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTc < 1:9939/250‑1 (MQ=255) ccACGTACGCTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGc > 1:398969/1‑251 (MQ=255) aCGCTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCggat > 1:166485/1‑251 (MQ=255) ccgcccTGACCGTAACCCCAGGTGACGCCGTCCGGGAACTGGAATACCTGAACGCCGGGTTTCAGCACCTGATCGCGGGTTGCGGTAGTGCTGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCAGGCCATTGACGTAGCCGTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCGTTTCGCCCggatgg < 2:663083/247‑1 (MQ=255) ccTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTGTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCggatgg < 2:3982/250‑1 (MQ=255) cTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTc > 1:305136/1‑251 (MQ=255) cccGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCGGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATTCCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑GTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg < 2:349655/250‑1 (MQ=255) cGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTa < 1:861164/251‑1 (MQ=255) gTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAc > 1:121800/1‑251 (MQ=255) cAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAc < 1:205536/251‑1 (MQ=255) aGGTGCCGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCGGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACt < 2:19955/251‑1 (MQ=255) aGGTGACGCCTGCCGGGAACTGAAATTCCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGGTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACt < 2:852868/251‑1 (MQ=255) gacgtcagtgcactgtaatcccttgaCGCCGGTTTTCAGCACCGGATCGCGGGTGGCGGTAGTGCCGTGTGGAATAGCAATTCCCGGGCAGAGTAACGTTTAGATTGGTTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTGCTGCTACATTCCCGGCCTGCACCTGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCGCGGATGGATGTCCTGTACGGATTACTGTAACAt < 2:152293/226‑1 (MQ=255) tCCGGGAACTGACATACCTGAACGCCGGTTTTAAGCGCCAGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACACTTGCCGAGGAACGTTGAGGTTTACTGTTCGCGCGCCAGCATGCCATTGGCGTAGCCTTCTGCTACATTACCGGCCTGCACCCGCGCCGCAGAGACCCTGCGAATCGCCTCCT‑TTTGTATCCGGCCTTTTCGCCCGGGTGAAGGTCCTGTACGGATAACTGGAACATAGtt > 2:613854/1‑251 (MQ=255) ccGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc > 1:349702/1‑251 (MQ=255) cagggAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGACGCAGCGACCTGGCGCAGCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGCTGTCCTGTACGGATAACTGGAACATAGttt > 2:285002/3‑250 (MQ=255) aattGAAATACGTGAACGCCGGTGTTCAGCAATGGATCGCGGGGGTCGTTAGGGTAGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGACAGCAGGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGACTTGTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcctc < 2:191/248‑1 (MQ=255) aaaTACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg < 1:345392/251‑1 (MQ=255) ccTGAACGCCGGTTTTCAGCACCTGATCTCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa > 1:534791/1‑250 (MQ=255) gcaCGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGACCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGCTGGATGTCCTGTACGGCTAAATGGACCATAGTTCTCCTCTCTTGATGAATTg > 2:692692/3‑251 (MQ=255) gAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTg > 1:870389/1‑251 (MQ=255) gAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTg < 1:47271/251‑1 (MQ=255) ggttgcAGCTCCTGATCGCGGGTGTCGGTAGTGTCGTGTGGAATAGCAATGCCATTGCCGAGGAACGTTGAGGTTTGCTGGTCGCGCGCCAGCATGCCATTGCCGTAGCCTTCTGCTACATTGCCGGCCTGCACCAGCGCCGCATCGTCCTGGCGATTCGCCTCTT‑TTTGTCTCCGGCCTTGTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTTAAACGAtt < 2:5930/246‑1 (MQ=255) tttcagacccTGATCGCGGTGGTCGGGAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTc < 2:487356/242‑1 (MQ=255) tttCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTc < 1:692692/250‑1 (MQ=255) gATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGATTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCctctctg > 1:524866/1‑246 (MQ=255) tCGCGGGTGTAGGTAGTGACGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGGTAACTGGAACATCGTTCTCCTCTCTTGCTGAATTGACAAGATTCAGCCTCTATgtaa > 2:408998/1‑247 (MQ=255) cgcgGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaa < 1:842856/251‑1 (MQ=255) gggTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaaaa > 1:485942/1‑250 (MQ=255) gTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCt > 1:29489/1‑251 (MQ=255) ggCCGTGTGGAATAGCCATACCAGTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTg < 2:236682/250‑1 (MQ=255) ccGTGTGGAATAGCAATACCATTGCCGAGGACCGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTCCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGc < 2:485942/251‑1 (MQ=255) tgGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGAAGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAgg > 2:323674/1‑251 (MQ=255) aaTAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGCAGCCGTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt < 2:563794/251‑1 (MQ=255) tACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACa > 1:90867/1‑250 (MQ=255) aTTGCAGAGAAACGTTGAGGTTTGCTGTTCTCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTAACGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTTTCTCCGGCCTTTTCGCCCGGATGGATGTCATGTACGGATAAATGGCACATAGTTCTCCTCTCTAGCTGAATTGAAACGATTCAGCCTATATGAGAAAAAAAGAGCCAACCTGGCTTAGGGTTaacgaataaatc > 2:581567/1‑241 (MQ=255) cGAGGAACTTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCt < 2:653293/251‑1 (MQ=255) aaCGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTGCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAAc < 2:201026/251‑1 (MQ=255) aCGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTATACTTCTGCTACATTACAGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCATGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTCTGAGAAAAAAAGCGCCAACCTGGCTTAGGGATAAAGACAAGATCTCGCTGAAACg > 2:296878/1‑251 (MQ=255) tggTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGCTGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGTTTCAGCCTCTATGCGAAAAAAAGAGCCAACCTGGCTTAGGGTTAAAGACAAAATAGCGATGAAACGTTTCa > 2:218892/2‑250 (MQ=255) aGGTTTGCTGTTCGCGCTCCAGCATGCCATTGTCGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCATCGACCTGTCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACGTGAACATAGTTCTCCTCTCTTGCTGAATTGAATCGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCaa < 1:840918/251‑1 (MQ=255) aGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGttcca < 1:448472/251‑4 (MQ=255) tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGa > 2:94610/1‑250 (MQ=255) gTTCTCGCGCCAGACTGCCACTTACGTTGCCTTCTGCTACTTTACCGGCCATCACCAGCGCCTCAGCGACCTGGCGAATTGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATTGATGTCCTTTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCGCTATGAGAAAAAAAGCGCCAACCTGGCTTAGTGTTAAAGACAAGATCGCGCTGAACCTTTTCAAGAAAGCata < 2:39612/251‑1 (MQ=255) cgcgcgCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGCTTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCataat > 1:693226/1‑250 (MQ=255) ccgcgcCAGCATGCCATTGACGTACCCGTCTGCTACATTACCGGCCTGCCCCAGCGCAGCAGCGACCTGGCTAATCTCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGGCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCACTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATAc < 2:249150/250‑1 (MQ=255) gcgcCAGCATGCCCTTGCCGTTGCCTTCTGCTACATTCCCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCAGAAACGTTTCAAGAAAGCATAATACtt < 2:398969/251‑1 (MQ=255) cccAGCAGGCCATTGACGTAGCCTTCTGCTACCTTAACGGCCTGCACCAGCGCCGCAGCGACCTTGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGCAACGTTTCAAGAAAGCATAATACTTCt < 2:693226/250‑1 (MQ=255) ccAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTg < 1:85943/251‑1 (MQ=255) cATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCACTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTc > 1:488487/1‑251 (MQ=255) aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACg < 1:371921/251‑1 (MQ=255) aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACg < 1:115774/251‑1 (MQ=255) gTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCAGCAGCGACATGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCATGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGTATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGGTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCacgg > 1:60678/1‑247 (MQ=255) aGCCTTCTGCTCCATTACCGGCCTGCACCAGCGCCGCAGCTACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGaa < 2:349702/250‑1 (MQ=255) tCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTATT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGcc < 1:123032/249‑1 (MQ=255) gCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTaa > 1:198755/1‑250 (MQ=255) attcccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTAt < 1:649971/247‑1 (MQ=255) ggCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCa > 2:48038/1‑250 (MQ=255) cacatgcgcCGCAGCGACTTTGCGAATCGCCTCT‑GTTTGTCGTCGGCCTTGTCGCCCGGATGGATGTCATGTACGGATAACTTGCACATAGTTCTCCTCTCTTTCTGAATTGAAACGATTCTGCCTCTATGAGAAAAAAAGCGCCAACCGGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTACAAGAAAGCATAATACTACTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGtta < 2:10107/246‑2 (MQ=255) cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGttt < 1:8794/251‑1 (MQ=255) cTGGCGAATCGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAAtttt > 2:409145/1‑251 (MQ=255) aTCGCCTCTT‑TTTGTATCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAt > 1:741160/1‑251 (MQ=255) cGCCTCTT‑TTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAttt < 1:48038/251‑1 (MQ=255) | CAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTT > NC_000913/2263195‑2263684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |