BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,878,536	G→A	Y528Y (TAC→TAT)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,878,536	0	G	A	97.1%	409.8 / 0.1	141	Y528Y (TAC→TAT)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (68/66); total (73/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 141 total aligned reads displayed.

ATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCC  >  NC_000913/3878291‑3878779

  taGCTCTTTCTGCATTTCTTTCGGATAACGCCCCTCCATACGATTGATCATTTTCCGCGTCGCGTT‑GTACTCAGATACCAGTTTCCCTAACGCTCCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACg                                                                                                                                                                                                                                                <  2:125750/249‑1 (MQ=255)

   aGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGATGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCGGAGCGAGATAGACGTg                                                                                                                                                                                                                                              >  1:452002/1‑251 (MQ=255)

    gCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGa                                                                                                                                                                                                                                             <  1:48786/251‑1 (MQ=255)

    gCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGa                                                                                                                                                                                                                                             <  1:419141/251‑1 (MQ=255)

        tttCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACcgc                                                                                                                                                                                                                                         >  1:658910/1‑251 (MQ=255)

             gCATTGCTTTCGGATAACGACGCTACATACGATTGATTATTTTCTGCGTCGCGTT‑GAACTCAGATACCAGTTTCTCTACCGCTTCGCCAGCCGATGCCGGTGCCCTAGAGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGAACCATCGCTTCGGCGTCTTTAATGTACTGTTCCTCCTTGACTTTCTTCACTTTGTACAGCCGCGGCTGAGTGATATAgtcgataccgctttcc                                                                                                                                                                                                                                    >  2:607354/1‑236 (MQ=255)

              cATTGCTTTCGGATCACGACGCTCCATACGATTGATAATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATAGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTAAAGCGGCGGCTGGACGGTTTAGACTGGACCGCGTTCa                                                                                                                                                                                                                                    >  2:791940/1‑250 (MQ=255)

               aTTGCTTTCGGATAACTACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAAc                                                                                                                                                                                                                                  >  1:401881/1‑251 (MQ=255)

                  gCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGAt                                                                                                                                                                                                                               >  1:200945/1‑251 (MQ=255)

                  gCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGAt                                                                                                                                                                                                                               >  1:187929/1‑251 (MQ=255)

                       cGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGGGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTCCAGCGGCGGCTGATCGATATATGCGTGACCGCGTTCAACGGTTTccc                                                                                                                                                                                                                          >  2:679123/1‑250 (MQ=255)

                        ggATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCgg                                                                                                                                                                                                                         >  1:254668/1‑251 (MQ=255)

                          aTAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGCTTTCCGGcc                                                                                                                                                                                                                       >  1:728114/1‑250 (MQ=255)

                               gacgCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGATCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTg                                                                                                                                                                                                                   >  1:845559/1‑250 (MQ=255)

                                 ggccccATCCGATTGATCATTTTCTGCGTC‑CGTTTGTACTCAGATTCCAGTTTCGCTAACGCTACGCCAGCCAATGCCGGGGCACTGGCGTTGGTGTGCATCGTTGCGCCGTCCAGCGCGATAGAGAGCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACAGCGTTCAACTATTTCCGGCATCTGACg                                                                                                                                                                                                                <  2:818647/247‑1 (MQ=255)

                                   ccacacgcccctcttacgatcttGCGTCGCGTT‑GTACACAGATACCAGTTTCGCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGAt                                                                                                                                                                                                              <  2:230502/229‑1 (MQ=255)

                                    tCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGAt                                                                                                                                                                                                              >  1:717284/1‑250 (MQ=255)

                                       aTACGAGTGATCATTTTCTGCTGCGGGTT‑GTACCCAGAATCCAGTTTCTCTACCGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCGTTAATGTACTGTTCCTGCTTGCCTTTCCTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGCCGATagaa                                                                                                                                                                                                          <  2:728114/251‑1 (MQ=255)

                                           gATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTGCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACATCGGCGGCTGAGCGATGTAGACGTGACCGCTTTCAACg                                                                                                                                                                                                                                 <  2:162328/224‑1 (MQ=255)

                                               gATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATTTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAgggc                                                                                                                                                                                                   >  2:63443/1‑248 (MQ=255)

                                               gATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTc                                                                                                                                                                                                   >  1:220600/1‑250 (MQ=255)

                                                 tCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTACTGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTTACGATAGAAGAAGGtaaa                                                                                                                                                                                                 >  2:809600/1‑247 (MQ=255)

                                                 tCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGATTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGCTTTCCGGCATCTGACGATAGAAGAAGGGCAAc                                                                                                                                                                                                >  2:248651/1‑251 (MQ=255)

                                                         tGCGTCGCGTT‑GTACTCCGATACCAGTTTCTCTACCGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGAt                                                                                                                                                                                                              >  1:230502/1‑229 (MQ=255)

                                                           cGTCTCGTG‑GTACTCAGATCCCAGTTCGTCTACCGTTTCGCCAGCCAATGCCGGTTCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTTATCCATCGCTTCGTCTTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGCCGATAGAAGAAGGTCAACAGCAGCGTAc                                                                                                                                                                                      <  2:595851/251‑1 (MQ=255)

                                                           cGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGGGCACTAGCGTTGGGGTGCAGCGTTGCGCCGTCCAGCGCGCTAGAGATCTGGTACTGATCCATCGCTTCGTCGCCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGCGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTAc                                                                                                                                                                                      >  1:383580/1‑251 (MQ=255)

                                                            gTCGCGTT‑GTACCCAGATCCCAGTTTCTCTAACGTTCCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCGTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACg                                                                                                                                                                                     <  2:759797/251‑1 (MQ=255)

                                                              cgcgTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGa                                                                                                                                                                                    >  1:46417/1‑250 (MQ=255)

                                                                 gTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGCCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACTATAGAAGAAGGTCAACAGCAGCGTACGAAtgt                                                                                                                                                                                >  1:819927/1‑251 (MQ=255)

                                                                  gttttACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGCTATAGACGTGACCGCGTTCAAAGGTTTCCGGCATCTGACGGTAGAAGAAGGTCAACAGCAGCGTACGAAtgt                                                                                                                                                                                >  2:477000/5‑251 (MQ=255)

                                                                     gTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCg                                                                                                                                                                             >  1:705515/1‑251 (MQ=255)

                                                                      tACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGGATGTGCgg                                                                                                                                                                            >  1:736838/1‑250 (MQ=255)

                                                                       actcacacaCCAGTTTCTCTACCGCTTCCCCAGGCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGTTCTGGTCCTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTTCTTGCCTTTCTTCGCTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCATCGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAg                                                                                                                                                                           <  1:188006/243‑1 (MQ=255)

                                                                       aCTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAg                                                                                                                                                                           <  1:615103/251‑1 (MQ=255)

                                                                           aGATACCATTTTCTCTATCGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTACAGCGCGATAGAGATCTGTTACTGATCCATCGCTTCGGCGTCTTTAATTTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGt                                                                                                                                                                       <  1:218726/251‑1 (MQ=255)

                                                                                 cAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCCCTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGCCGTGACCGCGTTCAACGATTTCCGGCCTCTGACGATAGAAGTAGGACCACCGAGGCGTACCAATGTGCGAGCCGtagaagg                                                                                                                                                                 >  2:640887/1‑245 (MQ=255)

                                                                                     ttCTCTAACGCTTCGCCAGCCACTGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATCTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTCCGAATGTGCGAGCCGTCGACTTCCGc                                                                                                                                                             >  2:114609/1‑251 (MQ=255)

                                                                                      tctcTAACGCTTCGCCCGCCAATGCCGGGGCACTTGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGAGAGAGACGTGACCGCGTTCAACGATTTCCGGCATATGACGATAGAAGTATGTCCACAGCAGCGTTCGAATGGGCTAGCCGTCGCCGTCCGc                                                                                                                                                             >  2:604885/1‑250 (MQ=255)

                                                                                        tctAACGCTTCGCCAGCAAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGCCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGTCCGAGTTCACAGATTTCCGGCCTCTGACGGTCAAAGAAGGTCAACAGCAGCGTACGAACGTGCGAGCCGTCGACGACCGCATc                                                                                                                                                          >  2:46011/1‑251 (MQ=255)

                                                                                        cctaacctttccccagccaATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCTCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACATCCGCATc                                                                                                                                                          <  2:163139/239‑1 (MQ=255)

                                                                                           aaCGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATc                                                                                                                                                          <  1:720784/248‑1 (MQ=255)

                                                                                             cGCTTCGCCAGCCAAGGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCa                                                                                                                                                     <  1:844389/251‑1 (MQ=255)

                                                                                              gtctCGCCAGCCAATGCCGGTGCACTGGGGTTGGTGTGACGCGTTGCGACGTTCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTTGGCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTGTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCat                                                                                                                                                    <  2:220600/248‑1 (MQ=255)

                                                                                              gCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGAGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCat                                                                                                                                                    >  2:289074/1‑251 (MQ=255)

                                                                                               acacGCCAGCCTATGCCGGTGTTGTTGCGTTGGTGTGCCGCATTGCGCCGTCCAGCGCGATAGAGATCTGGTTCTGATCCATCGCTTCGTAGTCTTTAATGTACTGTTCCAGCGTGCCTTTCTGCGCTTTGGACAGCGGCGGCTGGGCGATATAGACGTGACCGCGTGCAACGATGTCCGGCATCGGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGGCGTCGACGTCCGCATCGGTCatg                                                                                                                                                   <  2:697648/248‑1 (MQ=255)

                                                                                                     cagccaATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCTGCGACGTCCGCCTAGGTCatgatgatg                                                                                                                                             >  2:585889/1‑251 (MQ=255)

                                                                                                          aaTGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑t                                                                                                                                       <  1:701109/251‑1 (MQ=255)

                                                                                                               cggtgcctgtgtgTTGGTGTGCAGCGGGGCGCCGTCCAGCGCTAAAGATATCTGGTACTGATCCCTTGCGTCGTCGTCTTTAATGTTCTGTTCCTGCTTGCCTTGCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCATCAGCAGCGTACGAATGTGCGATCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGAt                                                                                                                                  <  2:301246/239‑1 (MQ=255)

                                                                                                                 gTGCCCTGGCGTTGGTGTGCAGCTTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCGTTAATGTACTGTCCCTGCTTGCCTTTCTTCCCTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATaa                                                                                                                                <  2:737454/251‑1 (MQ=255)

                                                                                                                  tGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAAc                                                                                                                               >  1:26184/1‑251 (MQ=255)

                                                                                                                   gCACTGGCGTTGGGGTGCAGCATTGCGCCGTCCAGCGCGATCGAGCTCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGTTATAGACGTGACCGCGTTCAACGATTTCCGGCCTCTGACGCTCGAAGACGGTCAACAGCAGCGTACGAATGTGCGAGCCGTTGACGTCCGCATCGGTTataatgatactgcttggttaacg                                                                                                                               >  2:141123/1‑236 (MQ=255)

                                                                                                                       tGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGCTTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGCGCCGTCGACGTCCGCATCGGTCATGATGAAGATGC‑TGGGATAACGccgt                                                                                                                          >  2:562570/1‑248 (MQ=255)

                                                                                                                        ggCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGtt                                                                                                                         >  1:640401/1‑251 (MQ=255)

                                                                                                                         gCGTTGGGGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTGGTACAGCGTCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGTATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGttt                                                                                                                        <  1:548148/251‑1 (MQ=255)

                                                                                                                              ggtttgCAGCGTTGCGCCGTCCAGTGCGATAGAGATCTGGTTCTGATCCATCGCTTCGTTGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCTGCTGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCTTCTGACGATAGAATAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGGCGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCg                                                                                                                   <  2:504238/247‑1 (MQ=255)

                                                                                                                                     aGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTa                                                                                                            <  1:63443/251‑1 (MQ=255)

                                                                                                                                       cGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATAGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACt                                                                                                          <  1:585889/251‑1 (MQ=255)

                                                                                                                                            cgcCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCa                                                                                                     <  1:424485/251‑1 (MQ=255)

                                                                                                                                             gcCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAc                                                                                                    <  1:248651/251‑1 (MQ=255)

                                                                                                                                                gTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGAc                                                                                                 <  1:693746/251‑1 (MQ=255)

                                                                                                                                                  ccAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGAcc                                                                                                <  1:195618/250‑1 (MQ=255)

                                                                                                                                                    aGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGAt                                                                                             <  1:21686/251‑1 (MQ=255)

                                                                                                                                                              ggaTCTGTTTCTGGTCCATCGCTTTGTCGTCTTGAATGTACTGTTCCTGCTTTCTTTTCTTCACTTATCACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGGACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGCGATAACGCCGTTTGTCCGGGTTGTACTCGTCACGACCGATACCCCAGCCa                                                                                   <  2:699656/250‑1 (MQ=255)

                                                                                                                                                               ggtCTGGTATTGAACCATCGCATCGGCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGTCGGCAGAGCGATATAGACGTGACCGCGGTCAACGATTTCCGGCATCTGACGATAGAAGAAGGGAAACAGCAGCGAACGCATGTGAGAGCCGTCGACGTCCGCATCGGTACTGCTGCTG‑GGCATGTGATAACGCAGTTTGTCCGGGTTGTTCTCGTCGCGACCGATACcaacgccc                                                                                   >  2:393684/3‑244 (MQ=255)

                                                                                                                                                                    ggTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCGGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAgcg                                                                               <  1:809600/249‑1 (MQ=255)

                                                                                                                                                                     gTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAgaagaa                                                                                                                                                                                                       <  1:93051/129‑1 (MQ=255)

                                                                                                                                                                     gTACTGATCCATCGATTCGTCGTCTTTAATGTACTGTTCCTGCTTGACTGTCTTCACTGTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCgg                                                                            <  1:423098/251‑1 (MQ=255)

                                                                                                                                                                      tACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGCATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACCGCCAAGCGCggg                                                                           >  2:83790/1‑250 (MQ=255)

                                                                                                                                                                      tACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCa                                                                                   <  1:715626/243‑1 (MQ=255)

                                                                                                                                                                      tACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTAAGAATGTGAGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTCCTCGTCCCGACCGATACccaagccaagttcgg                                                                            >  2:715626/1‑245 (MQ=255)

                                                                                                                                                                       aCTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGAGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTg                                                                          <  1:289074/251‑1 (MQ=255)

                                                                                                                                                                        cTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTg                                                                          >  1:153457/1‑250 (MQ=255)

                                                                                                                                                                          gATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGAt                                                                        >  2:665103/1‑250 (MQ=255)

                                                                                                                                                                          gATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGAt                                                                        >  1:203746/1‑250 (MQ=255)

                                                                                                                                                                            cccttcgtttctgcttcTTTAATGTACTGTTCCTGCTTTCCTTTCTTCCCTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATGTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAg                                                                     <  2:629904/236‑1 (MQ=255)

                                                                                                                                                                               aTCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTTAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCCGTTTGTCCGGGTTGTCCTCTGCACGCACGATACCCCAGCCAAGCGCGGGGATAAGCGt                                                                  >  2:520226/1‑251 (MQ=255)

                                                                                                                                                                                 ctcTTCGTCTTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACCGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCCGCGTACGCATGTGCGCGCCGTCGACGTCCGCCTCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGGGATAAGCGTCg                                                                >  1:501823/3‑251 (MQ=255)

                                                                                                                                                                                      cgtcttCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGAGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACt                                                           <  1:586503/251‑1 (MQ=255)

                                                                                                                                                                                       gtcgtcTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                          <  1:260019/251‑1 (MQ=255)

                                                                                                                                                                                       gtcgtcTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                          <  1:792830/251‑1 (MQ=255)

                                                                                                                                                                                        tcgtcTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTc                                                         >  1:471459/1‑251 (MQ=255)

                                                                                                                                                                                                   tttcTGTTCCTGCTTGCCTTGCTTCACTTTGTACAGCGGCGGCTGAGCGATACAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagag                                               <  1:503106/247‑1 (MQ=255)

                                                                                                                                                                                                    tACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGACGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGCagag                                               >  1:122455/1‑249 (MQ=255)

                                                                                                                                                                                                     tcTGTTCCTGCGTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATTCCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagagag                                             <  1:540754/249‑1 (MQ=255)

                                                                                                                                                                                                     aCTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGc                                            >  1:616634/1‑251 (MQ=255)

                                                                                                                                                                                                     aCTGTTCCTGCTTGCCTTTCCTCTCTTTGTACAGCGACGGTGGGGCGATATAGACGTGACCGCGTTCAACGATTGCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGGGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagagag                                             <  2:218617/250‑1 (MQ=255)

                                                                                                                                                                                                      cTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGACGGCTGAGCGTCATAGAACTCAACGCGTTACACGCTTTCCGGCATCCGAAGCTAGCAGAAGGACAAATGCGGCGGACGTATGCGCGAGCCGTCGCCGCCCGCATCGGTCCCGATGCAGATGC‑TGTGGTAACGCAGTTTGCCCGGGTTGTCCTCGTCAAGACCGATACCACCTCCAAGCGCGGCGTTAAGCGTGGcctcctcccgagacagacgc                                            >  2:804116/1‑232 (MQ=255)

                                                                                                                                                                                                        gTTCCTGCTTGCCTTGCTTCACTTTGTCCTGGGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACTATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATTTGCGAGCCGTCGACGTCCGCATCTGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTACCTGAGAAGAGAGCAt                                          <  2:834887/250‑1 (MQ=255)

                                                                                                                                                                                                              gCTTGCCTTCCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATc                                    <  1:604885/250‑1 (MQ=255)

                                                                                                                                                                                                                  gCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGCCCGCGTTTAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgc                               <  2:203746/251‑1 (MQ=255)

                                                                                                                                                                                                                   ccTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcg                              >  1:772449/1‑251 (MQ=255)

                                                                                                                                                                                                                           cccTTTGTACAGCGGCGGCTTAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTTCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                      <  2:254668/249‑1 (MQ=255)

                                                                                                                                                                                                                                tGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACg                 >  1:214689/1‑251 (MQ=255)

                                                                                                                                                                                                                                   aCAGCGGCGGCTGAGCGCTAAAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGCTGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGACGAGAGCATCTTCTCGAGGCGCGCTTTCTCGACGTTg              >  2:566338/1‑251 (MQ=255)

                                                                                                                                                                                                                                    cAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGa             <  1:763735/251‑1 (MQ=255)

                                                                                                                                                                                                                                        ggcggcTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt         >  1:833178/1‑251 (MQ=255)

                                                                                                                                                                                                                                        ggcggcTGAGAGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGACTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGCTAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGCGATAAGCGTCGCCACTTCCTGAGAAGAGCGCATCTTATCGAAGCGCGCTTTCTCGACATTGAGGAt         >  2:534596/1‑251 (MQ=255)

                                                                                                                                                                                                                                          cggcTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAttt       >  1:117674/1‑251 (MQ=255)

                                                                                                                                                                                                                                             cTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGCTAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAc    >  1:41294/1‑251 (MQ=255)

                                                                                                                                                                                                                                               gAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc  >  1:687914/1‑251 (MQ=255)

ATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTT‑GTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGC‑TGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCC  >  NC_000913/3878291‑3878779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`