Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 94,278 G→A A371A (GCG→GCA)  murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091394,2780GA98.3% 362.7 / NA 120A371A (GCG→GCA) murEUDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (57/59);  total (58/62)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCGGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTGACTTGCGCCGTTATG  >  NC_000913/94031‑94524
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         
gCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGgcagcg                                                                                                                                                                                                                                                        <  1:374346/251‑1 (MQ=255)
     aGCTCAAGTTGGGGCGATGGCGAAAGTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCt                                                                                                                                                                                                                                                   <  1:870463/251‑1 (MQ=255)
       cTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGCTCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGCCGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGGGGATTACGCGCATAAGCCGGGTGCACTGGAAAAAGCCTTACAGGCAGCGCGGCTGc                                                                                                                                                                                                                                                 >  2:286412/1‑251 (MQ=255)
            gTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGGCTGCACtgg                                                                                                                                                                                                                                            >  2:420531/1‑250 (MQ=255)
              tGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTAGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGc                                                                                                                                                                                                                                          >  1:140135/1‑251 (MQ=255)
                  gCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCAGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAACGCGGCTGCCCTGGGCgggg                                                                                                                                                                                                                                      >  2:672287/1‑250 (MQ=255)
                          gAAATTGAAAGCCATCTGTGGGGCGTTTTTAACGTCAGCACCCTGCTGCTCGCGCTTGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCGGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCtgtg                                                                                                                                                                                                                              <  2:759039/251‑1 (MQ=255)
                          gAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCtgtg                                                                                                                                                                                                                              <  1:317255/251‑1 (MQ=255)
                                 aaaGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTc                                                                                                                                                                                                                       <  1:768052/251‑1 (MQ=255)
                                    gCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCttt                                                                                                                                                                                                                    >  1:326328/1‑251 (MQ=255)
                                      cATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTATTTgg                                                                                                                                                                                                                  >  1:136248/1‑251 (MQ=255)
                                       aTCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGAAACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCCCTGTGCGGGCAAGCTGTGGTGTGTCTTTGGc                                                                                                                                                                                                                 >  2:520501/1‑251 (MQ=255)
                                          tGATGGGCGCTTTTACCGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGCAGTGTTCACTGCGCCAGGCAAACCGACGGGGGTGGGGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCTGCTGCACTGTCCGGGACAGCTGTGGTGTGTCTTTGGCtgt                                                                                                                                                                                                              >  2:780022/1‑251 (MQ=255)
                                          tGATGGGCGATTTTAACGGCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGACGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAGGCCTTACAGGCAGAGCGTCTGCACTGTGCGGACAGGCGGTGGGGTATCTTTGGCtgg                                                                                                                                                                                                              >  2:203820/1‑250 (MQ=255)
                                                  gcTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCg                                                                                                                                                                                                      >  1:89297/1‑250 (MQ=255)
                                                      ttAACGTCAGCAAGCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACGCGGCTATCCACTGGCTGATCTGCTG‑AAAGCCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATcg                                                                                                                                                                                                  <  2:304886/250‑1 (MQ=255)
                                                       tAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATcgcg                                                                                                                                                                                                >  1:112827/1‑251 (MQ=255)
                                                       tAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGGTCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATcgcg                                                                                                                                                                                                >  1:132965/1‑251 (MQ=255)
                                                        aaattcgcaagccagcttcgcgcgCTGGCGACA‑TGTGGGCCACCAGGCGATCCCCTTGCTGATCGGTTG‑AAAACCGCCGCGCATC‑TGCAACCGTGTTGCTGACGTATGTAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATcgcg                                                                                                                                                                                                <  2:760324/230‑1 (MQ=255)
                                                         aCGTCAGCACCCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGAt                                                                                                                                                                                              <  1:64530/251‑1 (MQ=255)
                                                         aCGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGAt                                                                                                                                                                                              >  1:340292/1‑251 (MQ=255)
                                                         aCGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGAt                                                                                                                                                                                              >  1:445069/1‑251 (MQ=255)
                                                           gTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGGGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCGGTGGTGGGTCTTTGGCTGTGGTG‑GCGATcgcggta                                                                                                                                                                                             >  2:625342/1‑247 (MQ=255)
                                                             cggcacccggctgctCGCGCTGGCGACACGGTTGGC‑ACTCGGCTATCCCCGGGCGGATCTG‑TGTAAAACCGCCGCGCGGCGTG‑AACCGGTTTGCGGATGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGGGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGAGCGCGATAAAg                                                                                                                                                                                          <  2:645013/242‑1 (MQ=255)
                                                             cAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGTTCGCGATaa                                                                                                                                                                                            >  2:708132/1‑249 (MQ=255)
                                                             cAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATaaa                                                                                                                                                                                           <  1:396859/250‑1 (MQ=255)
                                                              aGCAACCTGCTGCTCGCGCTGGCGACACTGGTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGCTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGGGGGGGTGGATTACGCGCCTACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGAAGGCTGTGGTGTGTCTTTGGCTGTGGGG‑GAGATCGCGGTAAAgg                                                                                                                                                                                         >  1:65726/1‑251 (MQ=255)
                                                                cAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGt                                                                                                                                                                                        >  1:839109/1‑250 (MQ=255)
                                                                 atcctgcggcccggccTGGCGCCACTGTTGGC‑ACTCGGCGATCCACTGGCGGATCTGCTT‑AAACCCGCCGCGCGTC‑TGCAACCGGTTTGCTGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGGGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGAAAGCTGAGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGTTAAAGGTaa                                                                                                                                                                                      <  2:854829/236‑1 (MQ=255)
                                                                         gctCGAGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGACGCGCGTC‑TGCAACCAGTTTGCGGGCGTGTGGAAGAGTTCAATGCGCAAGGCAAACCGACGGTGGTGGAGGATTACGCGCATACGCCGGCTGCACTGGAAAAAGCCTAACCGGCAGAGCGTCTGCACTGTGCGGGCAATGTTTGGTGTGTCTTTGGCTATGGTG‑GCGACTGCGATCAAGGTATGCGtgccc                                                                                                                                                                              >  2:51251/1‑247 (MQ=255)
                                                                                caggataccatattttc‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGATGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGCTAAAGGTAAGCGTCCACTGATggg                                                                                                                                                                       >  1:415453/17‑251 (MQ=255)
                                                                                   gCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGg‑cgc                                                                                                                                                                   <  1:780022/251‑1 (MQ=255)
                                                                                    cGACGCTGTTGGC‑ACCCGGCTATCCTCTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCa                                                                                                                                                                  <  2:643352/251‑1 (MQ=255)
                                                                                       cacTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCactt                                                                                                                                                               >  2:374857/1‑248 (MQ=255)
                                                                                           ggtGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGcc                                                                                                                                                            <  1:4598/248‑1 (MQ=255)
                                                                                                  aCTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGGGTGTCTTTGGCTGTGGGG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGt                                                                                                                                                     >  2:625940/1‑251 (MQ=255)
                                                                                                  aCTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGCAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGGTAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAAAGt                                                                                                                                                     >  1:856983/1‑251 (MQ=255)
                                                                                                    tCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTCCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGttt                                                                                                                                                   >  1:713182/1‑251 (MQ=255)
                                                                                                     cGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGtt                                                                                                                                                    <  1:420531/249‑1 (MQ=255)
                                                                                                     cGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGGGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGtttt                                                                                                                                                  >  2:263451/1‑250 (MQ=255)
                                                                                                      ggCTATCCACTGTCTGATCTGCTG‑ATAACCGCCGCGCGTC‑TGCATCCGGTTTGCGGACGTATGGAAGTGTTCCCTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTTTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGc                                                                                                                                                 <  1:780850/251‑1 (MQ=255)
                                                                                                            ccACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGCGTTTGCTGACGt                                                                                                                                           >  1:720401/1‑251 (MQ=255)
                                                                                                            ccACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGAGCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGt                                                                                                                                           <  1:828788/251‑1 (MQ=255)
                                                                                                                     gATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCggtg                                                                                                                                    <  1:455260/249‑1 (MQ=255)
                                                                                                                               aaaaCCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTggcggacgg                                                                                                                         >  1:745259/1‑250 (MQ=255)
                                                                                                                                 aaCCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGTCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGAGCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATa                                                                                                                       <  1:79475/251‑1 (MQ=255)
                                                                                                                                 aaCCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGGGGATTACGCGCATACGCCGGATGCACTGGAGAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCGGGGGTG‑GGGATCGCGGTACGGGTAAGCGTCCACT‑ATGGGGCGCAATTTCCGAAGGGTTTGCTGAGGGGGCggatgtggcagacgatg                                                                                                                       >  2:686962/1‑236 (MQ=255)
                                                                                                                                  aCCGCCGGGCGTC‑GGCAACCGGTTTGCGGACGTATGGAAGTGTTCATTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATaa                                                                                                                      <  2:706842/251‑1 (MQ=255)
                                                                                                                                        gcgcgTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGc                                                                                                                                        <  1:551457/227‑1 (MQ=255)
                                                                                                                                        gcgcgTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTG‑TGTGCGATCGCGATAAAGGTAAGCGTACACGGCTGTG‑CGCACTTGACGAAAGGTTTGCTGACGTGGCGGGGGTGGGGGACGaaaatccccg                                                                                                                >  2:515059/1‑242 (MQ=255)
                                                                                                                                            gTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGGGTGTCTTTGGCTGTGGAG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGACGAGTTTGCTGACGTGGCGGTGGGGAGGGACGACAACCCGCGGAcc                                                                                                            >  2:613655/1‑251 (MQ=255)
                                                                                                                                              gttgtacccGGTTGGCGGTCGTATGGAAGGGTTCCCGGCGCCGGGCAACACGACGGTGGGTGTGGACTACGCGCATACGCCGGATGCGCTGGAAAAAGCCTTACAGGCAGCGCGTGTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCg                                                                                                           <  2:455092/244‑1 (MQ=255)
                                                                                                                                                tGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGGACGGGCGCTAACCCGCGTACCgg                                                                                                          >  1:209386/1‑249 (MQ=255)
                                                                                                                                                   aaCCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGGACGGACGCTAACCCGCGTACCgaaga                                                                                                       >  1:375554/1‑250 (MQ=255)
                                                                                                                                                    aCCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGGCGGAAGATAAACCCCGGACCgaagaa                                                                                                      >  1:613078/1‑250 (MQ=255)
                                                                                                                                                    aCCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAAc                                                                                                     <  1:520501/251‑1 (MQ=255)
                                                                                                                                                            gCGGACGGATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGc                                                                                              <  2:273103/250‑1 (MQ=255)
                                                                                                                                                                aCGTATTGAAGTGTTCACTGCGCCAGGCAAACCGACGGGGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCcatca                                                                                         <  2:493836/251‑1 (MQ=255)
                                                                                                                                                                 cGTATGGAGGTGTTCACTTGGCCAGGAAAACCGACGGTGGTGGTGGAGTACGCGCATGTGCCGGATGCACTGGAAAAATCCTTACAGGCAGCGCGTCTGCACTTTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGTTCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCTGTTGTGACGTCCGATAATCCGCGTACCGAATAACCGCGTGCcatcat                                                                                        <  2:443154/251‑1 (MQ=255)
                                                                                                                                                                  gTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCatcatc                                                                                       <  1:216634/251‑1 (MQ=255)
                                                                                                                                                                    aTGGAAGTTTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCAtcatca                                                                                      <  2:708102/250‑1 (MQ=255)
                                                                                                                                                                    aTGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCAtcatca                                                                                      <  2:536981/250‑1 (MQ=255)
                                                                                                                                                                     tGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGCAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTACGCGTCAACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGGGGGGACGATGACCCCCGGGCCGAGGAACCGCGTGccgaacgcaac                                                                                    >  2:868800/1‑242 (MQ=255)
                                                                                                                                                                      ggAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGGCGGACGATAAACCCCGTACCGAAGGACCGCGGGCCATCATCAACg                                                                                   >  1:871343/1‑251 (MQ=255)
                                                                                                                                                                           tgtTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGGACGGACGGTAAACCGCGGACCGAAGAACCGCGTGCCATCATCAACGAGAtt                                                                              >  1:209180/1‑251 (MQ=255)
                                                                                                                                                                            gtTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATAtt                                                                              <  1:351992/250‑1 (MQ=255)
                                                                                                                                                                               cACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTgg                                                                          <  1:337677/251‑1 (MQ=255)
                                                                                                                                                                               cACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTgg                                                                          <  1:529631/251‑1 (MQ=255)
                                                                                                                                                                                        aGGCAAAGCGACCGTGGTTGTGGATTACGCGCATACGCCGGATGCACTGGAAAACGCCTTCCAGGCAGCGCTTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCTATAAAGTTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGATTTTGCTGACGTGGCGGTGGTGCCGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATg                                                                 <  2:203601/250‑1 (MQ=255)
                                                                                                                                                                                        aGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGGCGATAACCCGCGTACCGAAGAACCGCGTGCCCTCATCAACGATATTCTGGCGGG‑GATGt                                                                >  1:108021/1‑251 (MQ=255)
                                                                                                                                                                                        aGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGt                                                                <  1:682260/251‑1 (MQ=255)
                                                                                                                                                                                        aGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGTACGGACGATAACCCGCGTACCGAAGAACCGCGAGCCCTCATCAACCATATTCTTGCGGG‑GATGt                                                                >  1:213612/1‑251 (MQ=255)
                                                                                                                                                                                         ggCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGGGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGtt                                                               <  1:596365/251‑1 (MQ=255)
                                                                                                                                                                                            aaaCCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGGACGGACGATAACCCGCGTACCGAAGAACCGCGGGCCATCATCAACGATATTCTGGCGGG‑GATGtttg                                                             >  1:748125/1‑248 (MQ=255)
                                                                                                                                                                                              cccGACTGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCGTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGGTTGCTTACGTGGCGGTGGTGACGGACTATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATg                                                          <  1:380876/250‑1 (MQ=255)
                                                                                                                                                                                                cGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGGGGGGACGGACGATAAACCGCGTACCGAAGAACCGCGGGCCATCATCAACGATATTCTGGCGGG‑GATggttaatgcc                                                        >  1:384769/1‑242 (MQ=255)
                                                                                                                                                                                                 gACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGCCGTGGCGGCGGGGACGGACGGTAACCCGCGTACCGAAGAAACGCGTGGCAATATCAACGATATTCTGGCGGGGAATGTT‑GATGCCg                                                       >  1:498787/1‑251 (MQ=255)
                                                                                                                                                                                                  aCGGTGGTGGTGGATTACGCGCATACGCCGGATGCTCTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGTCGGG‑AATGTTAGATGCCgg                                                      <  2:83583/251‑1 (MQ=255)
                                                                                                                                                                                                    ggtgggggtGGATTCCGCGCATATGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTTGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGAc                                                    <  2:140354/251‑1 (MQ=255)
                                                                                                                                                                                                     gtggtggtgGATTACGCGCATTCGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGTTCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACa                                                   <  1:510476/251‑1 (MQ=255)
                                                                                                                                                                                                       ggtggtggATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGCTAACCCGCGTACCGAAGAAACGCGTGCCATCATCCACGATATTCTGGCGGG‑GATGTTTGTTGCCCGACATg                                                 >  1:514420/1‑251 (MQ=255)
                                                                                                                                                                                                       ggtggtggATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATg                                                 <  1:708132/251‑1 (MQ=255)
                                                                                                                                                                                                        gtggtggATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATGc                                                <  1:870087/251‑1 (MQ=255)
                                                                                                                                                                                                        gtggtggATTACGCGCATACGCCGGATGCACTGGAAAAAGACTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATGc                                                <  1:511762/251‑1 (MQ=255)
                                                                                                                                                                                                          ggttgATTACGCGCATCCGGCGGATGCACTGGAAAAAGCCTTACAGGCGGCGCGTCAGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGGT‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGGTTGCTGACGTGGCGGTGGTGACGGACGATAACCCTCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTATATGCCGGACATGCCa                                              <  2:763210/247‑1 (MQ=255)
                                                                                                                                                                                                              gATTACGCGCATACTCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGGGGG‑GATTTTAGATGCCgggccttcccaagt                                          >  1:843749/1‑239 (MQ=255)
                                                                                                                                                                                                                    gcgcATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGGGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATGCCAAAGTGATGGa                                    <  1:867442/251‑1 (MQ=255)
                                                                                                                                                                                                                        aTACGCCGGATGCACTGGAAAAAGCCTTACAGGCCGCGCGTCTGCCCTGTGCGGGCAAGTTGTGGTGTGTCTTTGGCGGTGGTG‑GGGCTGGCGATAAAGGTAAGCGTCCACTGCTGGG‑CGCAATTGCCGACGAGTTTGCTGACGTGGGGGGGGGGACGGACGAAAACCCGCGTACCGAAGAAACGCGTGCCCTCGTCAACGGTCTTCTGGCGGG‑TATATTACATGCCCGACaaccagacgcggaggaaga                                 >  1:807289/1‑232 (MQ=255)
                                                                                                                                                                                                                        aTACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGGAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑GATGTTAGATGTCGGCCATGCCCAAGAGGTGGAAGGc                                >  1:599769/1‑251 (MQ=255)
                                                                                                                                                                                                                        aTACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑GATGTTAGATGCCGGACATGCCCAAGTGaagggaggg                                >  1:468028/1‑243 (MQ=255)
                                                                                                                                                                                                                         tACGCCGGATGCCCGTGAAAAGGTCTTACAGGAGGCGCGTCGTCACTGTTCGTGCAAGCTGTGGTGTGTCTTTTGCTGTGGTT‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCTAAGAACCGCGTTCCATCATCAACGATAGTCTGGCGGG‑AATGTTAGATTCCGGACATGCCAAAGTGATGGAAGGc                                <  2:754685/250‑1 (MQ=255)
                                                                                                                                                                                                                          aCGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGcc                               <  1:43069/250‑1 (MQ=255)
                                                                                                                                                                                                                          aCGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTATGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGc                                <  1:34869/249‑1 (MQ=255)
                                                                                                                                                                                                                               ggATGCACTGGAAAAAGCCTAACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGGGGTG‑GCGATCGCGATAAAGGTAATCGTCCACTGAGGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCAGCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTg                         <  2:136248/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTTGATGCCGGACATGCCaaaagggtgggaggccgggctggagc                     >  1:62307/1‑228 (MQ=255)
                                                                                                                                                                                                                                   gCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCGTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGAGGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATGCTGGCGGG‑AATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAg                      <  2:108021/250‑1 (MQ=255)
                                                                                                                                                                                                                                          aaaaaGCCGTACTGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGATGTGGTT‑GCGATCGCGATAAAGGTAAGCGTCCTCTGATGGG‑CGCAATTGCCGAAGAGTTGGCTGTCGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTGACt              <  2:256706/251‑1 (MQ=255)
                                                                                                                                                                                                                                             aaGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTGACTTgc           <  1:693914/251‑1 (MQ=255)
                                                                                                                                                                                                                                                ccTTACAGGCAGGGCGTCTGCACTGGGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACTATATTCTGGCGGG‑AATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTGCCTTGCGcc        <  2:839109/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      aGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑GGTGTTAGATGCCGGACATGCCCAAGTGATGGAAGGCCGTGGTGGAGCGGGGACTTGCGCCGGTAtt  >  1:181936/1‑250 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         
GCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGC‑ACTCGGCTATCCACTGGCTGATCTGCTG‑AAAACCGCCGCGCGTC‑TGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCGGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTG‑GCGATCGCGATAAAGGTAAGCGTCCACTGATGGG‑CGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGG‑AATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTGACTTGCGCCGTTATG  >  NC_000913/94031‑94524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑