Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3630C.89.7% 237.6 / 19.4 68intergenic (‑490/+919)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base . (50/11);  total (53/15)
Fisher's exact test for biased strand distribution p-value = 3.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01
*NC_0009132,173,3640C.89.7% 236.9 / 20.1 68intergenic (‑491/+918)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base . (50/11);  total (53/15)
Fisher's exact test for biased strand distribution p-value = 3.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

GCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCG  >  NC_000913/2173123‑2173613
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                         
gCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGCTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCCCACAAAAAGCCATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTcc                                                                                                                                                                                                                                                >  2:689534/1‑251 (MQ=255)
   ttAGCCGCCGGTTGGGTGTGCAAACCAATAGTTGGCGTCGCGATCCACAGGGTGATGCTAATAATGATGACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCAGCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAACCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCca                                                                                                                                                                                                                                              <  1:689534/250‑1 (MQ=255)
        cgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCCGGCTTGCCGATACCACC‑‑GCCGTATGTCCCagcagcc                                                                                                                                                                                                                                        >  2:605895/1‑250 (MQ=255)
        cgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTAACCGGCACAAAAACAGCAATTAAAATGGTGTGTGGGATAAAAATCAGGCTTGCCGA‑AC‑ACACCGCCGTATGTACcagcaga                                                                                                                                                                                                                                         >  2:27232/1‑249 (MQ=255)
            aGTTGGGTGTGCAAACCCATCGATTGCGTCGCGATCCACAGGGTGATGCTCATAATGCTGACACACGCGGTTAAGGTGCGGAACAGATTTCCACGATGCCCGGCGACCGCCATCGCCACGAAAAAGCCGATGGTGGCAAGA‑TCGACAAAAGGCAGCACCTGAGTACCCGGCACACACACAGCTATTAAAATGGTGAGTGGGATAAAAATCGGGCTTGCCGATACCACC‑‑GCCGCATGTACCAGCAGAAACGc                                                                                                                                                                                                                                    >  2:435337/1‑251 (MQ=255)
                        aaaCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTAAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGcc                                                                                                                                                                                                                        >  2:437615/1‑251 (MQ=255)
                               tAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                                                                                                                                 >  1:48424/1‑251 (MQ=255)
                                         cgcgATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGCTCAAGGACAATCAGGAACTCCTGAc                                                                                                                                                                                                       >  2:479549/1‑251 (MQ=255)
                                         cgcgATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAc                                                                                                                                                                                                       >  1:391747/1‑251 (MQ=255)
                                            gATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGATGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACACACAGCAATTAAAATGGCGAGTGGGATAAAAATCAGGCTTGCCGATACCAAC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGAccgc                                                                                                                                                                                                    >  2:337341/1‑251 (MQ=255)
                                                ccaaGGGTAATGCTCATAATGATGCCCCCCGAGATTAAGGGGCGGAACAGATTCCCCCGATGCACGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGAAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGaa                                                                                                                                                                                                <  1:284617/248‑1 (MQ=255)
                                                cacaGGGGGATGCTCAAAAGGATGACACCCGAGATTAAGGTGCGGAACAGATTCCCACGATGCCGGGCGACCGCAATCGCCACGAAGAAGCCGAGGGTGGAAAGA‑CCGCCAAACGGCAGCACCTGATTACCCGGCACAAAAACAGCAATAAAAATGGTGAGTGGGATAAAAACCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGaa                                                                                                                                                                                                <  1:386004/251‑1 (MQ=255)
                                                  caGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACt                                                                                                                                                                                              >  1:392905/1‑251 (MQ=255)
                                                           gCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGt                                                                                                                                                                                      >  1:77197/1‑250 (MQ=255)
                                                            cTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGCTTACCCGGCCCACAACCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTgca                                                                                                                                                                                     >  2:680538/1‑248 (MQ=255)
                                                             tCATAATGATGACACCCGCGATTAAGGTGCGGAACAGCTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaa                                                                                                                                                                                    >  1:244842/1‑250 (MQ=255)
                                                              cATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCACGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGAAAAc                                                                                                                                                                                  >  2:208122/1‑251 (MQ=255)
                                                               aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACg                                                                                                                                                                                 >  1:690883/1‑251 (MQ=255)
                                                                       gaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACg                                                                                                                                                                           >  1:763792/1‑249 (MQ=255)
                                                                       aacacCCGAGATTAAGGGGTGAAACAGATCTCCCCTATGCCCGGCGCCCGACATCGCAACCAAAAAGCCGACGGTGGCAAGA‑TCGACAAACGGAAGCTCCTGATTCCCCGGCACAAAAACAGCAATTAAAATGGGGAGTGGGATAAAAATCAGGCTTGCCTATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAg                                                                                                                                                                         <  1:65729/250‑1 (MQ=255)
                                                                         cacCCGGGATTAAGGTGCGGAACAGCTTTACACGATGCACGGCGACCGCAATCGCCAAGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGACCCTGCTTACCAGGCACACAAACAGCATTTACAGTGGTGAGTGGGATAAACATCAGGCTTGCCGATAC‑‑CCCCGCCGTCTGTCCCAGCAGCTCCGCCGGATCACGGACAATCAAGTACTCCTGACCGACGCACTTCGCCTGTAAAcaacgacgcacc                                                                                                                                                                       >  2:636032/1‑240 (MQ=255)
                                                                            cgaagaTTAGGGTGCGGAACAGATTCCCCCGATGCACGGCGCCCGCCTTCGCCACAAAGAAGCCGTTGGTGGCAAGA‑TCGCCAAGCGGCAGCACCGGATTACCCGGCACACAAACAGCAATAAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAC‑‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGACCTTCGCCTGTAAACGACTACGAGCCTGc                                                                                                                                                                    <  1:123798/248‑1 (MQ=255)
                                                                                   tAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCgc                                                                                                                                                             >  1:56255/1‑250 (MQ=255)
                                                                                     gggTGCGGAACAGATTCCCACGATGCACGGCGCCCGCCATCGCCACGAAGAAGCCGAGGGTGGCAAGA‑TCGCCAAACGGCAGCACTGGATTACCCGGCCCACAAACAGCAATTAAAATGGGGAGTGGGATAAAAACCAGGCTTGCCGATTCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGTATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATg                                                                                                                                                           <  1:568336/250‑1 (MQ=255)
                                                                                       gTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAACCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGACACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTCAACGCCTACGAGCCTGCTTCGCGATggg                                                                                                                                                         >  1:870736/1‑251 (MQ=255)
                                                                                                  aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGCACTTCGCCTGTAAACGACt                                                                                                                                                                              >  2:437633/1‑219 (MQ=255)
                                                                                                  aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                                                                                                                                              >  1:552138/1‑251 (MQ=255)
                                                                                                  aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACATGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                                                                                                                                              >  2:342813/1‑251 (MQ=255)
                                                                                                        aCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTCGCGATGGGCGTTAAACCATCCatg                                                                                                                                         >  2:41818/1‑250 (MQ=255)
                                                                                                          gagccagggCGCCCGCAATCGCCACGAAGACGCCGAGGGTG‑CAAGAAGCGCAAAAGGGAACCCCCTGTTTACCCGGCCCAAAAACAGCAATAAAAAGGGTGGGTGGAAAAAAAATCAGTCTGGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGTAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgatg                                                                                                                                      <  2:777688/244‑1 (MQ=255)
                                                                                                                cGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGttt                                                                                                                                 >  1:375891/1‑250 (MQ=255)
                                                                                                                  gCGACCGCCATCGCCACGAAGAAGCCGAGGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCAAACAAACAGCAATAAAAATGGTGAGTGGGATAAAAATCAGGCTTCCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                                                                                                                              <  1:688516/251‑1 (MQ=255)
                                                                                                                   cgcccGCCATCGCCACAAAGAAGCCGATGGGGGCAAGA‑CCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGATTGGGATAAAAATCAGGCTGGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATc                                                                                                                             <  1:578268/248‑1 (MQ=255)
                                                                                                                   cGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATc                                                                                                                             >  1:700528/1‑251 (MQ=255)
                                                                                                                   cGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGACTGCTTAGCGATGGGCGTTAAAACATCCATGATGGGTTTAATc                                                                                                                             >  1:721857/1‑251 (MQ=255)
                                                                                                                    gACCGCCATCGCCACGACGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCACTCAGGAACTCCTGACCGCCGGACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAACCCATCCATGATGGGTTTAATcc                                                                                                                            >  2:485387/1‑250 (MQ=255)
                                                                                                                    gACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATc                                                                                                                             >  2:251895/1‑250 (MQ=255)
                                                                                                                       cGCCATCGCCCCGAAGAAGCCGATGGTGGCAAGA‑TCGCCACACGGCAGCACCTGATTACCCGGCACACCAACAGCCATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCAAC‑‑GCCGAATGTCCCAGCAGCAACGCCGGATCACGGCCAATCAGGAACTCCTGACCGCCGAAACTCGCCTGTCAACGACTACTAGCCTGCTTAGCGATGGGCGTTACACCATCCATGATGGGTTTAATCcccc                                                                                                                         >  2:538712/1‑251 (MQ=255)
                                                                                                                         ccATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACACGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATAAGGATTGCCGATACCACC‑‑GCCGTATGTACCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCAGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGt                                                                                                                       >  1:493076/1‑251 (MQ=255)
                                                                                                                                 aCGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt                                                                                                                 >  1:357164/1‑249 (MQ=255)
                                                                                                                                   gaagaaGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagc                                                                                                              >  1:358633/1‑250 (MQ=255)
                                                                                                                                      gaagCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagcagc                                                                                                           >  1:331368/1‑250 (MQ=255)
                                                                                                                                           cGATGGTGGAAAGA‑TCGCCAAACGGCAGCCCCTGATTACCCGCCACACAAACAGCAATTAAAATGGTGATGGGGATAAAAACCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGAGGGGTTTAATCACCCGGGGCATTAGCAGCATCACt                                                                                                     <  1:281777/251‑1 (MQ=255)
                                                                                                                                            gATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTg                                                                                                    >  1:467502/1‑251 (MQ=255)
                                                                                                                                                    aaaGA‑TAGCCAAACGGCAGCACCTGATTACCCGGAACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGAAGCATCAATGCCGACGtt                                                                                            >  2:389669/2‑251 (MQ=255)
                                                                                                                                                     aaGA‑TCGCAAACCGGCAGCACCTGATACCCCGGCACACAAACAGAAATTAAAATGGGGAGGGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTGAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGttt                                                                                           <  1:550349/251‑1 (MQ=255)
                                                                                                                                                        a‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGCTGGGTTTAATCAACCGTGGCTATAGCAGCATCACTTCTGCCGttttt                                                                                         >  2:600734/1‑250 (MQ=255)
                                                                                                                                                          tCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTAc                                                                                       >  1:670437/1‑251 (MQ=255)
                                                                                                                                                            gCCAAACGGCAGGACCTGATTACCCGGCACACAAACAGCAATTAAAATGGAGAGGGGGATAAAAAACAGGCTTGACGCTACAACC‑‑GCCGTCAGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCCCCGACCTTCACCTGAAAACGACTACGAGCCTAACTCGCGAAGGGAGTTATACCATCCATCATGGGTATAATCACCCATGACCTTAGCAGCATCACTACCGCCGttttcac                                                                                       >  2:324587/1‑246 (MQ=255)
                                                                                                                                                              aaaccggcagcacaggataccccGCCACAAAAACAGAAATAAAAAGGGTGAGTGGAAAAAAAACCAGGCTTGCCAATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                   <  1:342813/231‑1 (MQ=255)
                                                                                                                                                                   ggCAGCACCTGATTACCCGGCACACACACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATAACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTg                                                                              >  2:346195/1‑251 (MQ=255)
                                                                                                                                                                                    cGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGACCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCGGCTGCAATACACCTTTGACAt                                                             >  1:356322/1‑251 (MQ=255)
                                                                                                                                                                                          acaacaggaaataaaaaggggaaGTGGGAAAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGCCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAc                                                       <  1:405961/229‑1 (MQ=255)
                                                                                                                                                                                             aacaacAATAAAAAGGGGGAGTGGGATAAAAACCAGCCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcg                                                    <  1:27232/251‑1 (MQ=255)
                                                                                                                                                                                                  cAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGCTGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCCAACCACCTTTGACATCGTAACCCGCGAgg                                               >  2:523756/1‑251 (MQ=255)
                                                                                                                                                                                                  cAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACAGCCCGCTGAAATACACCTTTGCCATAGTAACCCGCAAgg                                               >  2:800128/1‑251 (MQ=255)
                                                                                                                                                                                                          aTGGTGAGTGGGATAAAAACCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAACCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGAt                                       <  1:251895/251‑1 (MQ=255)
                                                                                                                                                                                                                aGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc                                   >  1:286989/1‑249 (MQ=255)
                                                                                                                                                                                                                       tAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATc                          >  1:601931/1‑251 (MQ=255)
                                                                                                                                                                                                                        atcaaTCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGTACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGACCCTGCTTAGCGATGGGCGTTAAACCATCCATGAAGGGTTTAATCACCCGTGGCAGTAGCAGCATCACTCCCGTAGCTTTTACCGTCAGCTGCAATACACCTTTGACAACGAACCCCGCGAGGTAGCCGATactaaaccccaacc                         >  2:166894/4‑238 (MQ=255)
                                                                                                                                                                                                                        aaaaaTCCGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGAAGCAACGCCGGATCAAAGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACGAGCCAGCTTAGCGATGGGCGTTAAACCATCCAAGCTGTGTTTAATCACCCGTGGCATTCGCAGCATAACTGCCGCCTTTTTTAACGCCAGCTACAATCCACATACGACAACGTAACCCGCACGGATCCCGATGAGCAACCCCATc                          >  2:686989/1‑250 (MQ=255)
                                                                                                                                                                                                                         aaaaTCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGCACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac                        >  1:357680/1‑251 (MQ=255)
                                                                                                                                                                                                                           caTCAGGCTTGCCGATACCAAC‑‑GCCGCGTGTCCCAGCAGCAAAGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGAAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCCTCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCCGCTGCAATACACCTTTGACAACGTAACCCGAGCGGATGcccagaatccgacacttccca                       >  2:832663/2‑231 (MQ=255)
                                                                                                                                                                                                                               aGGCTTGCCGATACCACC‑‑GCCGTATGTCCCCGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCCTCACTGCCGCCGTTTTTACCGCCCGCTGCAATACACCTTTGACATCGTAACCCGAGAGGATGCCGATAATAGGCACCATCACAAAAcc                  >  2:801480/1‑251 (MQ=255)
                                                                                                                                                                                                                                ggCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAccc                 >  2:594499/1‑251 (MQ=255)
                                                                                                                                                                                                                                cgggcgtgccgatcccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGCCCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACaaaa                    <  1:485387/233‑1 (MQ=255)
                                                                                                                                                                                                                                      ccGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGccgccg                                                                                              >  2:256575/1‑168 (MQ=255)
                                                                                                                                                                                                                                       cGATACCACC‑‑GCCGTATGTCCCAGCGGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTg           >  1:428665/1‑250 (MQ=255)
                                                                                                                                                                                                                                       cGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAccccagggg           >  2:131679/1‑244 (MQ=255)
                                                                                                                                                                                                                                          aatccccccGCCGTATGTCCCAGCAGCAACGCCGGACCAAGCCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGAc         <  1:846186/246‑1 (MQ=255)
                                                                                                                                                                                                                                                 ccgccgTCTGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCTCCGTTTTTACCGCCAGCTGCAATAAACCTTTGACATCGTAACCCGCGAAGATGCCGATAATCACACCCATCCCAAAACCCACGGTGACCGGCTcc  >  2:274401/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 ccgccgTATGACCCAGCAGCAACGCCGGACCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCg  <  1:4594/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                         
GCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGA‑TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCG  >  NC_000913/2173123‑2173613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑