Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,975,224	G→A	A34V (GCG→GTG)	cheA ←	chemotaxis protein CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,975,224	0	G	A	94.7%	289.2 / NA	98	A34V (GCG→GTG)	cheA	chemotaxis protein CheA
Reads supporting (aligned to +/- strand):  ref base G (2/2);  major base A (49/40);  minor base C (3/2);  total (54/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGC  >  NC_000913/1974986‑1975470                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                        ggcATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGc                                                                                                                                                                                                                                              >  1:192619/1‑250 (MQ=255)       cGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAAccc                                                                                                                                                                                                                                       >  2:211301/1‑250 (MQ=255)        ggCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCaaaccc                                                                                                                                                                                                                                        >  1:145254/1‑246 (MQ=255)          ctctTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGCTGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGc                                                                                                                                                                                                                                     >  1:812933/1‑250 (MQ=255)              tGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCACCTCCTTTGATCGTGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCCATTCCGGGTCAAAAACCAGCAAAtg                                                                                                                                                                                                                                >  2:92111/1‑251 (MQ=255)               gCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGTCATCTGGCCCTTCCGGCTGCAAAACCACCACAtgc                                                                                                                                                                                                                               >  1:515346/1‑251 (MQ=255)                        tataAGCGTCGGTCTGTTCTTGCCTGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCGCCAt                                                                                                                                                                                                                       <  2:265880/250‑1 (MQ=255)                        tataAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCAt                                                                                                                                                                                                                       <  1:120254/250‑1 (MQ=255)                         ataAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGAAAATGCTGCTCCATGt                                                                                                                                                                                                                     >  1:393921/1‑251 (MQ=255)                           aaGCGTCGAGCTGTTCTTGCATGAGGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTGGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTc                                                                                                                                                                                                                    <  2:131537/250‑1 (MQ=255)                              cGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAAGGTCGGTGTTGAGTTTCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTGTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGcc                                                                                                                                                                                                                <  2:753356/251‑1 (MQ=255)                                 cGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAAc                                                                                                                                                                                                             >  1:155007/1‑251 (MQ=255)                                   aGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCAGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGCGGTTTCCTGCCAAACGCTGCAGCCAAAAGTTCCTACCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGCTGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAACACCAGCCAATGGTGCTCCATGTCAGcccac                                                                                                                                                                                                             >  2:177070/1‑246 (MQ=255)                                    gCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGGGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGCTGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGGACTTCCGGCTGCAAAACAAGCa                                                                                                                                                                                                                                    >  2:268628/1‑225 (MQ=255)                                    gCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGGGCGGCATCTGGCACTTCCGGCTGCAAAACCAGCa                                                                                                                                                                                                                                    <  1:268628/225‑1 (MQ=255)                                            tGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCg                                                                                                                                                                                                  <  1:295902/250‑1 (MQ=255)                                            tGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTCTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGc                                                                                                                                                                                                 >  1:163460/1‑251 (MQ=255)                                               atgatgTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTtc                                                                                                                                                                                              <  1:813571/251‑1 (MQ=255)                                                tgatgTCCTTCGGTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACATCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCCTCTGGCACTTCCGACTGCAAAACCAGCAAATGCTGCTCACTGTCAGCCACCA‑GTTCGTCCGCTtcc                                                                                                                                                                                             >  2:594202/1‑250 (MQ=255)                                                 gatgTCCTCCGTTGCCAAAAACATAGCGAGAATGTCGGGGTGGAGTTGCATCTCACCTCGTC‑GGTTTGCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCCCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCAATTCCGGCGGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCTGCtcca                                                                                                                                                                                             <  2:604724/250‑4 (MQ=255)                                                   tgTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCCGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTtcatc                                                                                                                                                                                           <  2:431745/250‑1 (MQ=255)                                                      ccTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCaaaa                                                                                                                                                                                       <  1:834495/251‑1 (MQ=255)                                                       cTTCGTTTCCAACAAAAGATTGCTCATGTCGGTGTTGAGTTGCATCTCACCTCGTATGGATT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAACGGTCCTGACCCTCCTTTGATCGAGTGGGCAACCAGAACGATGGACTTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTTCTCCATGTCAGCCAACA‑GTTCTGCCGCTTCATCaaaa                                                                                                                                                                                       >  2:855282/1‑250 (MQ=255)                                                       cTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCaaaa                                                                                                                                                                                       >  1:747259/1‑250 (MQ=255)                                                       cTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCaaaa                                                                                                                                                                                       >  1:83600/1‑250 (MQ=255)                                                        ttCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCCCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCaaaaa                                                                                                                                                                                      >  2:368300/1‑250 (MQ=255)                                                         tCGTTTCCAAAAACAGATTGATAATGTCGGTTTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCaaaaaa                                                                                                                                                                                     <  1:134409/250‑1 (MQ=255)                                                           gTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGt                                                                                                                                                                                  >  1:646378/1‑251 (MQ=255)                                                            tttCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGCTGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGAACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCCTCAAAAAAtttc                                                                                                                                                                                 >  2:504853/1‑248 (MQ=255)                                                             ttCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCCCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACC‑GTTc                                                                                                                                                                                                       >  2:328310/1‑228 (MQ=255)                                                             ttCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTc                                                                                                                                                                                                       <  1:328310/228‑1 (MQ=255)                                                                        gATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCg                                                                                                                                                                     >  1:580056/1‑251 (MQ=255)                                                                            gaaaagttCGGTGTATAGTTGCATCCCTCCTCATCTGGGTG‑CATCGAGAAGTTTTGCCAGCAGATGCGTGTTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGACCTTCCTGCGATTGAGTGGGCAGCTCGAAAGATTGCATTCAATTGTGCTGCATCTGGCACTTCCGGCTGCAATACTAGCAAATGCGTCTCCATGTCAGCCAACA‑GTTCGTCCGCTACATCAAAAAATGTCTGATAAAAATCGCtt                                                                                                                                                                  <  2:846976/243‑1 (MQ=255)                                                                             aTAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTtat                                                                                                                                                                <  1:307034/251‑1 (MQ=255)                                                                             aTAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTACGCTTCATCAAAAAATGTCTGATAAAAATCGCTtat                                                                                                                                                                <  1:397219/251‑1 (MQ=255)                                                                              tAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTtat                                                                                                                                                                >  1:80038/1‑250 (MQ=255)                                                                                        gtTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTc                                                                                                                                                      <  1:453549/250‑1 (MQ=255)                                                                                         ttGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAGGTTCCTGCCCCTCCTTTGATCGAGTGGGCATCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCAc                                                                                                                                                    >  1:422828/1‑251 (MQ=255)                                                                                          tGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCAcc                                                                                                                                                   >  1:485028/1‑250 (MQ=255)                                                                                           gAGTTGCATATCACCTCGTCTGGCTT‑CATCGAGCAGGTGTTCCATCAGATGCGGGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAAACGCTTATATCAATGATCACGc                                                                                                                                                  >  2:224056/1‑251 (MQ=255)                                                                                                gCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTc                                                                                                                                              >  2:367136/1‑250 (MQ=255)                                                                                                gCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCa                                                                                                                                             <  1:367136/251‑1 (MQ=255)                                                                                                  aTCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCa                                                                                                                                                                                                                   >  1:674259/1‑180 (MQ=255)                                                                                                  aTCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCa                                                                                                                                                                                                                   <  2:674259/180‑1 (MQ=255)                                                                                                   tctcACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCt                                                                                                                                          >  1:428742/1‑251 (MQ=255)                                                                                                      cACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTc                                                                                                                                         <  1:443394/249‑1 (MQ=255)                                                                                                      cACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTc                                                                                                                                         <  1:864262/249‑1 (MQ=255)                                                                                                        ccTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTtat                                                                                                                                                                >  1:697994/1‑224 (MQ=255)                                                                                                        ccTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTtat                                                                                                                                                                <  2:697994/224‑1 (MQ=255)                                                                                                             tCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCt                                                                                                                                >  2:465321/1‑251 (MQ=255)                                                                                                              gacgcccttcccatctaGGTTTTCCAGCAGTTGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCATCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGTTTCGGCt                                                                                                                                <  2:797808/235‑1 (MQ=255)                                                                                                               tGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGAATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTATCACCTCGGTTCGGcgg                                                                                                                               >  2:812332/1‑248 (MQ=255)                                                                                                                ggttt‑CATCGAGCAGGTTTTCCATCAGAGGCGTGGTTTCCGGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTGTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCGGCAAAACCAGCAAATGCTGCTCCATGTCAGACAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCt                                                                                                                                <  2:671960/245‑1 (MQ=255)                                                                                                                 gCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATg                                                                                                                            <  1:245400/251‑1 (MQ=255)                                                                                                                   tt‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGCAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGATCACGCTGTCACATCGGTTCGGCTGATGGc                                                                                                                          >  2:465445/1‑251 (MQ=255)                                                                                                                      cATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCAt                                                                                                                        >  1:116611/1‑251 (MQ=255)                                                                                                                            gCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACCCTGTCACCTCGGTTCGGCTGATGGCATTATggg                                                                                                                  >  2:25005/1‑251 (MQ=255)                                                                                                                             cAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAACAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAACATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTggg                                                                                                                  >  1:51814/1‑250 (MQ=255)                                                                                                                              aGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCATCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCt                                                                                                                                >  1:797808/1‑235 (MQ=255)                                                                                                                                gTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAac                                                                                                               >  1:313216/1‑250 (MQ=255)                                                                                                                                               cgaggTTTCCTGCAAAACGCTGAAGACAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGAAAAACAAGCAAATGCTGCTACATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAACTCGCTTATATTCATGCTCACGATGTCCCCTCGGTTCGGCTGCTGGCATTGTGGGAACACTGACCAGTGGTGcc                                                                                               >  2:478001/4‑250 (MQ=255)                                                                                                                                                 tGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGc                                                                                                                                                                                                                                     <  2:607790/116‑1 (MQ=255)                                                                                                                                                 tGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGc                                                                                                                                                                                                                                     >  1:607790/1‑116 (MQ=255)                                                                                                                                                  ggTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTGTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAAcc                                                                                            <  2:288609/251‑1 (MQ=255)                                                                                                                                                     ttCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTc                                                                                          <  1:504853/250‑1 (MQ=255)                                                                                                                                                            aaaaCGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAACCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGttttt                                                                                  >  2:344065/1‑251 (MQ=255)                                                                                                                                                                    gAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAggg                                                                            <  1:106997/249‑1 (MQ=255)                                                                                                                                                                    gAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGGCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAggg                                                                            <  1:514841/249‑1 (MQ=255)                                                                                                                                                                      aGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCt                                                                        <  1:575343/251‑1 (MQ=255)                                                                                                                                                                      aGCCAAAAGTTCCTGCCCCTCCTTTGAACGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGCCTGATGGCATTGTGGGCACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCt                                                                        >  1:442521/1‑251 (MQ=255)                                                                                                                                                                        caaaaaGTTCCTGCCCCTCCTTGGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTa                                                                      <  2:674907/249‑1 (MQ=255)                                                                                                                                                                                tCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACCCTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTc                                                               >  1:404653/1‑250 (MQ=255)                                                                                                                                                                                 ccTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCAt                                                             <  1:211301/251‑1 (MQ=255)                                                                                                                                                                                   tGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGAGCGCTTa                                                                      >  1:14249/1‑240 (MQ=255)                                                                                                                                                                                   tGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTAGGAACACTGACCTGGGGTGCAACCTCAGGTTTTTCCAGGGCGCTAACTGGCTCATTc                                                           >  2:261288/1‑251 (MQ=255)                                                                                                                                                                                   tGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTCCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGAGCGCTTa                                                                      <  2:14249/240‑1 (MQ=255)                                                                                                                                                                                       cctcctTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGc                                                       <  1:619808/251‑1 (MQ=255)                                                                                                                                                                                          cctTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCttt                                                    <  1:855282/251‑1 (MQ=255)                                                                                                                                                                                          actTTGATCGAGGGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCAGCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTTATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCt                                                      <  2:490418/248‑1 (MQ=255)                                                                                                                                                                                               gATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCg                                               <  1:92111/251‑1 (MQ=255)                                                                                                                                                                                                    aGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTc                                          >  1:480331/1‑251 (MQ=255)                                                                                                                                                                                                     gTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCACTCTGGCTTTCGGCGTTTTc                                          >  1:378594/1‑250 (MQ=255)                                                                                                                                                                                                      tGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCAt                                        >  1:246216/1‑251 (MQ=255)                                                                                                                                                                                                                gAAAGCTGGCATGCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCCGCACATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCCAAAAATGTCTGATAAAAATCGATTATAACCATGCTCACGCTGTCACCTCGGTTCGGATGATGGCCTTGTGGGAAAACTGACCTGTGGTGCACCCTCAGGTTTTACCAGGGCGCTTACTGGCTCATTCTGGCTTTCGCCGTTTTCCTGCACAATGGc                              >  2:383903/1‑251 (MQ=255)                                                                                                                                                                                                                   aGATGGCATTCAATTAGTCGGCATCTG‑CACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCGTCATCAAAAAATGTCGTATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGTCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGt                          <  2:699798/251‑1 (MQ=255)                                                                                                                                                                                                                       ggCATTCAATTTTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTc                        <  1:313960/250‑1 (MQ=255)                                                                                                                                                                                                                          aTTCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCt                    >  2:148790/1‑251 (MQ=255)                                                                                                                                                                                                                          aTTCAATTGTTCGGAATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTACATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGGGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTTTTCGGCt                    >  1:594491/1‑251 (MQ=255)                                                                                                                                                                                                                           ttCAATTGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCtt                   >  1:48321/1‑251 (MQ=255)                                                                                                                                                                                                                             taaTTGTTCGGCACGTGGACCTTCCGGCTGCAAAACCCGTAAAGGCTGCTCCATGTGATGCAACA‑GTTCGGCCACTTCATCAAAAAATGTCTGATAAAAAGCGCTTATATCCATGCTCACGCTGTCACCTCGGTCCGGCTGATGGCATTGGGGTAACACTGACCTGTGGTGGAACCTCAGGCGTTTCCAGGGCGCGTACTGGCTCAGTCTAGCTTTCGGCTTTTTCATGCAAAAGGGCCTGTTCGGCttg                  <  2:339268/249‑1 (MQ=255)                                                                                                                                                                                                                                 tGTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCttgtttg              <  2:428742/250‑1 (MQ=255)                                                                                                                                                                                                                                  gtgcGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCGCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCttgtttgt             <  1:494491/247‑1 (MQ=255)                                                                                                                                                                                                                                  gTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTc                                                   >  2:45895/1‑212 (MQ=255)                                                                                                                                                                                                                                  gTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTc                                                   <  1:45895/212‑1 (MQ=255)                                                                                                                                                                                                                                  gTTCGGCATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTtgtttgtt            >  1:113160/1‑251 (MQ=255)                                                                                                                                                                                                                                  gTTCGGCATCGGGCCGTTCCGGCGGAAAAACCAGCAAATGCTGTCCCTTGCCAGCCA‑CATGTTCGTCCGCTCCATCAAAAAATGTCTGTTAAAAACCCCTTATACCCATGCTCCCGCTGTCCCCCCGGTCCGCTTGATG‑CATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTc           <  1:115484/251‑1 (MQ=255)                                                                                                                                                                                                                                        cATCTGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTCTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTAc      >  1:803648/1‑251 (MQ=255)                                                                                                                                                                                                                                            tGGCACTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACcagc  >  1:870738/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                        GGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT‑CATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAGCCAACA‑GTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGC  >  NC_000913/1974986‑1975470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑