Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,715	A→T	S377C (AGT→TGT)	ydiF →	putative acetate‑CoA transferase YdiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,715	0	A	T	95.8%	128.6 / ‑1.2	49	S377C (AGT→TGT)	ydiF	putative acetate‑CoA transferase YdiF
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (22/24);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01

CATTATTCGAAATGCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTACCGGAAATTACTTTCAGCGGAAAAATCGCTCTCG  >  NC_000913/1776470‑1776961                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       cATTATTCGAAATGCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGtttt                                                                                                                                                                                                                                                   <  1:863333/251‑1 (MQ=255)       tCGAAATGCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGCTGTTTGTTATTTGTGTTTTGCTGa                                                                                                                                                                                                                                              >  1:71460/1‑250 (MQ=255)        cGAAATGCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGaa                                                                                                                                                                                                                                             <  1:541198/250‑1 (MQ=255)              gCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGAc                                                                                                                                                                                                                                       <  1:179564/250‑1 (MQ=255)                  aaaGGCGCGGTGGGGAATGTCGGCGTCGGTCTTGCTGACGGCATTGTCCTGGTCGCCCAAGAAGAAGGTTGTTCTGATGCCTTTATTCTGACGGTAG‑AAACAGGACCGATTGGCGGAATTACTTCACAAGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCAATCTGGATATTAAGTACCAGTGTGATTTTTATCACGGAGGCGGTCTGGATGATTGATATTTGTGTTTTGCTGacgccgcacagc                                                                                                                                                                                                                                   >  1:76865/1‑239 (MQ=255)                  aaaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGc                                                                                                                                                                                                                                   >  1:76924/1‑250 (MQ=255)                         gggTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAAc                                                                                                                                                                                                                           <  1:23146/250‑1 (MQ=255)                         gggTGGGGAATGTCGGCGTCGGTATTGCTGACGGCAGTGGCCTGGTCGCCCGAGAAGAAGGTTGGGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCGGAAGTCGACCAGCACGGTAAc                                                                                                                                                                                                                           <  2:713793/250‑1 (MQ=255)                         cgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGTCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAAc                                                                                                                                                                                                                           >  1:177210/1‑251 (MQ=255)                         cgGTGGGGAATGGCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAAc                                                                                                                                                                                                                           <  1:66864/251‑1 (MQ=255)                          ggTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGGCGACCGAGAAGAAGGTTGTGCTGTTGACTTTATTCTGACGGTAG‑AAACAGGTCCGGTTGGCGGGATTACTTCACATGGGACCGCCTTTTGCGCGACAGTGAATACCCGTGCCCTTCTGGATATGACGACCCAGTTTAATTTTTATCACGGTGGCGGTCTGGATATTTGTTATTTTGTTTTTGCTGAAGTCTACCaccaagttac                                                                                                                                                                                                                            >  2:315604/1‑240 (MQ=255)                             ggggAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCg                                                                                                                                                                                                                       >  1:752491/1‑251 (MQ=255)                                  aTGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTg                                                                                                                                                                                                                  <  1:636513/251‑1 (MQ=255)                                                   gCTGACGGCATTGGCCGGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAT‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTaa                                                                                                                                                                                                 <  1:291278/251‑1 (MQ=255)                                                     tgacggccatgtcctagTCGGCCGAGAAGGAGGTTGTGCTGATGACTTTATTCTGACGGTAGCAAAC‑GGTCCGATAGGCGGAATTACTTCACAGGGGTTAGCCTGTGGCGCGAACGGGAATACCCGCGCACTTCTGGATATGCCGTTCAAGTATGATTGTTCTCACGGTGGCGGTCTGGGTGTTTGTTATTTGTGTTTTTCTGAAGTCGACCAGCACGGTAACGTAGGCGGGCATAAATTCAATTGTaaa                                                                                                                                                                                                >  2:541867/17‑250 (MQ=255)                                                                 ccTGGTCGCCCGAGAAGAAGGTTGTTCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCg                                                                                                                                                                                   <  1:624003/251‑1 (MQ=255)                                                                  cggggctcccGAGAAGAAGGTTGTGCTGATGACTTGATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTTCGTCACAGGGGATCGCCTTTGGCGCGAACGTGAATTCCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAATTCATGGGCACCgg                                                                                                                                                                                  <  2:834892/244‑1 (MQ=255)                                                                    ggTCGCCAGCGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGACTTACTTCACAAGGGATCGCCTTTGGCGCGACCGTGAATACCCGGGCCATTCTGGATATGACGTCCCAGTTTGTTTTTTATCACGGAGGCGGTCTGGGTGGTTGTTCTTTGTGTTTTGCTGAAGTCGACCAGCCAGGAAACGTAGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCggcg                                                                                                                                                                                >  2:808957/1‑249 (MQ=255)                                                                               aagaagGTTGTGCTGATGACTTTATGCTGACGGTAG‑AAACAGGTACGATTGGCGGAATTATTTCACATGGGATCGCCTTTGGCTCGAACGTGAATACCCGTGCCCTTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATTGTAAAATCATGGGCACTGGTGGATTTATTGat                                                                                                                                                                     <  2:689402/251‑1 (MQ=255)                                                                                agaagGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGCTTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGACCGTGAATACCCGGGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCCCGGTAACGTCGGCGGGCATAAATTCAATGGAAAAATCATGGGCAACGGAGGGTTTAtttat                                                                                                                                                                     >  2:739034/1‑247 (MQ=255)                                                                                  aagGTTGGGTTGATGACTTGATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTGCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTTCCAGTTTGATTTTTATCACGGTGTCGGTCTGGATGTTTGTTATTTGTTTTTTGCTGAAGTCGACCAGCACGTTAACGTCGGCGTGCATAAATTCAATGGTAAAAGCATGGGCTCCGGTGGATTTATTGATATc                                                                                                                                                                  <  2:735375/251‑1 (MQ=255)                                                                                    ggTTGTTCTGATTACTTTATTTTGACGATAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTTCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTGGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAg                                                                                                                                                                <  1:354777/251‑1 (MQ=255)                                                                                    ggTTGGGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAg                                                                                                                                                                <  2:71460/251‑1 (MQ=255)                                                                                         tgCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTATGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCCCCGGTGGATTTATTTATATCAGTGcc                                                                                                                                                            >  2:508035/1‑250 (MQ=255)                                                                                                   tttATTCTGACGGTAG‑AAACAGGTCCGATTGCCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGAGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCAAGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCgaaga                                                                                                                                                  >  1:247440/1‑250 (MQ=255)                                                                                                    ttATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCgcagaa                                                                                                                                                 >  1:253013/1‑250 (MQ=255)                                                                                                           gACGGTAA‑ACACAGGTCCGATTGGCGGAATTACTTCCCAGGGGATAGCCTTTGGAGCGGACATGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGAGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGAACAGCACGGTAAAGTCGGCGTGCATAAATTCAATAGTAACATCATGGGCACCGGGGGACTTTTTGAAATCAgagaacatttgaagaaagaccttt                                                                                                                                         >  2:866696/1‑228 (MQ=255)                                                                                                                       cAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTTTGCGGcac                                                                                                                               >  1:576172/1‑250 (MQ=255)                                                                                                                        aGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACAt                                                                                                                             <  1:261818/251‑1 (MQ=255)                                                                                                                              gATTGGCGGAATTACTTCAAAGGGGATCGCCTTTGGCGCGAACGTGAATACACGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTg                                                                                                                       >  1:282457/1‑251 (MQ=255)                                                                                                                                     ggAACTACTTCACAGGTGATCGCCTTTGGCGTGAACGTTAATACCCGTGCTATTCTGGATATTACGTCCCAGTTTGAGTTTGATCACGGTGGCGGTCTGGATGTTTCTTATTTGTGTTTTGCTGTAGTCGACCAGCACGGTAACGTCGGCGTGCATAAAGTCAATGGTAAAATCATGGGCTCCGGTGGATTTATTGATATCAGAGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTACCTGCGGGCAg                                                                                                                <  2:659000/251‑1 (MQ=255)                                                                                                                                      gAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGCAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGcggt                                                                                                               >  2:290135/1‑248 (MQ=255)                                                                                                                                                 caGGGGATTGCCTGTGGCGCGAACGTGAATACCCGTGCCTTTCTGGATATGACGTTACAGTTTGAGTTTTATCACGGTGGCGGTCGGGATGGTTGGTATGTGTGTTGTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGAGTTAGTGAGATCAGTGCCACTTCGAAGAAAATCATGTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGa                                                                                                    <  2:734203/251‑1 (MQ=255)                                                                                                                                                          gCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGa                                                                                           <  1:138444/251‑1 (MQ=255)                                                                                                                                                            ccttGGCGCGAACGTGAATTCCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGGGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTTAAAACAGAAATTACCGACg                                                                                         <  1:824978/249‑1 (MQ=255)                                                                                                                                                             tttGGCGCGAACGTGAATACCCGGGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTCACGTCGGCGTGCATAAATTCAATGGTAAAATAATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCCCATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGCCgg                                                                                        >  2:818204/1‑251 (MQ=255)                                                                                                                                                                ggCGCGAACGTGAATACCCGTGCCATTATGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAACAACAGAAATTACCGACGGCa                                                                                      >  1:633575/1‑250 (MQ=255)                                                                                                                                                                     gAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATgg                                                                                                                                                                                                    >  2:658533/1‑135 (MQ=255)                                                                                                                                                                       aCGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGAGTGCATAAATTCAATGGTAAAATCATGGGCACCGGAGGATTTATTGATATCAGTGCCACTTCGAAAAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAACACAGAAATTACAGACGGCAAATTAAat                                                                              >  1:789820/1‑251 (MQ=255)                                                                                                                                                                             aTACCCGTGCCAATCTGGATATGACGTCTCAGTTTGATTTGTATCACGGTGGAGGTCTGGATGTGTGTTATTTGTGATTGGCTGAAGTCGACCAGCACGGTAACGGCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGTTGGATATATTGATATCAGGGCCACTGCGAAGAAAATCATTTACTGCGGCACATTAACTGCGGGCAGTTTAAAAGCAGAAATTACCGACGGCAAATTAAATATCGTc                                                                        <  2:5587/251‑1 (MQ=255)                                                                                                                                                                                cccGTGCCATTTTGGATATGACGTCCCTGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAAGGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCCCTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAACTACCGACGGCAGCTTAAATATCGTCCag                                                                     <  2:459200/251‑1 (MQ=255)                                                                                                                                                                                cccGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTcc                                                                       >  2:50378/1‑249 (MQ=255)                                                                                                                                                                                             ggATATGACGGCTCCGTTGGGTTTTTATTACGGTGGCGGGTTGGATGTTTGTTATTTTTGTTTTGCTTAAGGCGACCATCACGGTAACGGCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCCCATTAACTTCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTg                                                         <  2:114271/250‑1 (MQ=255)                                                                                                                                                                                                         cgagagcgATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAATTATCGTCCAGGAAGGACGGGGGAAGAAATTTATTc                                            >  2:211976/8‑251 (MQ=255)                                                                                                                                                                                                           aGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGCAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCgg                                          >  1:820227/1‑251 (MQ=255)                                                                                                                                                                                                                     ttATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTACCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTACCg                                 <  2:627215/250‑1 (MQ=255)                                                                                                                                                                                                                           cGGTGGAGGTCTGGATGTTGGTTATTTGTGTTTTGCTGAAGGCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTACCGGAAATTa                          <  2:752491/251‑1 (MQ=255)                                                                                                                                                                                                                                 cGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATAACGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTACCGGAAATTACTTTc                     >  1:699201/1‑250 (MQ=255)                                                                                                                                                                                                                                                    ttGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTACCGGAAATTACTTTCAGCGGAAAAATCGCTCTcg  <  1:211976/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CATTATTCGAAATGCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAG‑AAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTACCGGAAATTACTTTCAGCGGAAAAATCGCTCTCG  >  NC_000913/1776470‑1776961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑