| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,912,074 | G→A | S532F (TCC→TTC) | glmS ← | L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,912,074 | 0 | G | A | 93.3% | 223.8 / 4.7 | 76 | S532F (TCC→TTC) | glmS | L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (2/3); new base A (42/28); total (45/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01 | |||||||||||
ATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGAC > NC_000913/3911834‑3912322 | aTTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGttt < 1:160604/251‑1 (MQ=255) gacccaaccttcaCCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACAGGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGGTTTTCCa < 2:771892/238‑1 (MQ=255) tCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCa > 1:77271/1‑250 (MQ=255) ccGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCg > 1:673888/1‑251 (MQ=255) gATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTAGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCgg > 2:183059/1‑251 (MQ=255) ttttGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTg > 1:403268/1‑251 (MQ=255) tttGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCCTCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAAATGACCGCCACGCGCGCGCACTTCTTCAATGTGGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGc > 2:707266/1‑251 (MQ=255) gCCAGGTTAAGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGCAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaac > 2:60213/1‑251 (MQ=255) cAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaaca > 2:173883/1‑250 (MQ=255) aCGCGTCTGGTAAAAGTCGGTGCCTTTGATCAGCGAGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACATCT‑TCCACATACGGCATCTCGATGATGTGCATGTTATCGCTACTTACACAACCACCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGGGCGACCTTCTTCACTGTTGAATTTCAGTTTATCCAGCAATTCGTTGTACGGGGCAACAACAATa > 2:792902/1‑250 (MQ=255) aCGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATa < 1:173883/250‑1 (MQ=255) ggTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCGGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCata < 1:280847/251‑1 (MQ=255) tCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACATCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATc > 2:517559/1‑251 (MQ=255) gCCTTTGATAAGCGCGAAATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCTCCTCT‑TCCACATGCGGCATCTCGATGAGGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTa < 2:370500/251‑1 (MQ=255) gACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCgg > 2:275909/1‑250 (MQ=255) aGCCAGCAGCTGCAGCGGCAAGCTGTAGCAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATATCGATGATGTGCATGATATAGCTATTTACAAAAACCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTTTTCGGTGCAACAAAAATAACCGGCATATCTGCATCACTTAGCGCCAGCGGACCGTGTTTAAGtt > 2:597181/1‑251 (MQ=255) gcagcTGCAGCGGAACGGTGTAGAAGACCGGTGCAATCACCTCT‑TCCACATGGGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCca < 2:463216/251‑1 (MQ=255) cTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTAGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCcagca > 2:627910/1‑250 (MQ=255) aGCGGAACGGTGTAGAAGATCGGTGAAATTACACCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAGCCCGCATCCTGATCGTCGAAGACATACAAATGACCGCCACGCGCGAGCACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTAGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATACATTAGAGCCAGCGGACCGTGTTTCCGTTCGCCTGCAGCGtc > 2:508400/1‑250 (MQ=255) gAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCt > 2:755389/1‑251 (MQ=255) gAACGGTGTAGAAGAACGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGGGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGATCTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATGTCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCt < 1:822309/251‑1 (MQ=255) gggTGTAGAAGATCGGTTCAATCACC‑CTGTCCCCATGTGGCATCCCTATGATGTGCATGTTATCGCTTCTTACAAAACCCGAATCCTGTTCGGCGAAGACATACAACGGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCa < 2:610796/250‑1 (MQ=255) tgtAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCg > 1:844281/1‑251 (MQ=255) gtAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCg < 1:436558/250‑1 (MQ=255) gtAGAAGATCGGGGCAATCACCTCT‑TCCACATGCGGCATCTCGATGAGGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCg < 2:404260/250‑1 (MQ=255) gtAGAAGAGCGGTGCAATCACCTCT‑TCCACAGTCGGCATCTCGATGATGTGCATGTAATCGCTCCTTCCAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCg < 2:630004/250‑1 (MQ=255) agaagaTCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTg < 1:626596/250‑1 (MQ=255) agaTCGGTGCAATCACCTCT‑CCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATg < 2:620695/251‑1 (MQ=255) aTCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGt > 1:527075/1‑250 (MQ=255) aaTCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGAtct > 1:692886/1‑251 (MQ=255) cACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCtt < 1:802173/251‑1 (MQ=255) ccACATGCGGCCTCCCGAGGATGTGCATGTTATCGCTACTTACAAAACCCGCAGCCTGATCGGCGAAGATATTCAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATACGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGGTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTc < 1:294341/251‑1 (MQ=255) cctGCGGCATCTCGATGATGGTCATGTTATCTCTACTTACAAAAACCGCATCATGATCGGCGAATACATACATCTGACCGCCACGCGCTCTAACTTCGTCAATGTTGAATTTCAGTTTTGCCAGCAATACTTTGGTCGGTGCAACAACAATAACCGGCATATCGGCAGCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTGCAGCGTGAATGTAAGAGATCTCTTTCAACTTCaaa < 2:40131/249‑2 (MQ=255) aTGCGGCATCTCGCTGATGTGCATGTTATCGATACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAACGCCAGCGGACCGAGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAATAGATCTATTTCAACTTAAATg > 2:631416/1‑251 (MQ=255) ggcgcactttcGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACACCTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATg < 2:485518/241‑1 (MQ=255) gCATCTAGATGATGAGCATGTTATCGCCACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGCGATCTATTTCAACTTCAATgcgctt > 2:801177/1‑249 (MQ=255) ccGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAg < 1:275909/250‑1 (MQ=255) gatgatGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAgcg > 1:553236/1‑251 (MQ=255) gtgCATGTTATCGCTACTTACCACACCCGCATCCTGATCGGCGCAGACATACACCTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCATGAATGTAAGAGATCTATTTCAACTTCAATGCGCCTTCCAGCGCGATTg > 2:488475/1‑251 (MQ=255) gCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTggg > 1:673367/1‑251 (MQ=255) cGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATc < 1:517559/250‑1 (MQ=255) gCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCCCCTTCCAGCGCGATTGGGTACTGATCGc > 2:378628/1‑251 (MQ=255) cTACTTACAAAACCCGCATCCTGATCGGCGACGACATACCACTGACCGCCACGCGCGCGAACTTCTTCACTGCTGCATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACACCAATAACCGGCATATCGGCATCAATTAGCGTCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCCACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGcc > 1:831761/1‑251 (MQ=255) tACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCCGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATACCCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCCATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGc > 1:65343/1‑251 (MQ=255) aaaaCCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGcccc > 2:767999/1‑250 (MQ=255) aaaCCCGAATCCTGATCGGAGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGACTGTAAGCGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGccc > 2:506421/1‑249 (MQ=255) cccGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGa > 1:873891/1‑250 (MQ=255) cGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAAc > 1:3098/1‑250 (MQ=255) tCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAgcgc > 1:41653/1‑251 (MQ=255) ccTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGcgcg > 1:786663/1‑251 (MQ=255) tGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCAGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGACCAGCGCGTg > 1:226110/1‑251 (MQ=255) gATCGGCGAAGAAATACAACTTACCGCCACGCGCGCGAATTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGAGTGGGGACTGATCGCCACGGCCCAGGAACAGCGCGTg < 2:795777/250‑1 (MQ=255) ggCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGTCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCACTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGtt > 1:98285/1‑251 (MQ=255) aaGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATATCCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTGCCAGCGCGATTGGGGACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTc < 1:506421/251‑1 (MQ=255) aTACACCTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAgaga < 2:77271/251‑1 (MQ=255) aCTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACAGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGCTCGCCACGGCCCAGGAACAGCGAGTGATGTTTGTCAGGGAAAtct > 1:550629/1‑251 (MQ=255) cTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAAtctt > 1:530619/1‑251 (MQ=255) tGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAAtcttc > 1:89059/1‑251 (MQ=255) cgtacggcactggcgcgAACTTATTCAAAGTTAAATTGCATTTTTGCCAGCAATTCGTTGTCCGGTGCAACAACAATAAGCGGCATAACGGCATCAATTAGCGCCAGCGGTCCGTGTTTCAGTGCGCCAGCAGCGTAGGCTTCAGCGGGAATGGAAGAGATCTCTTTCAACGGCAATGCTCCTTCCAGCGTGATTGGGTACTGAACGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAAtctta < 2:241473/239‑2 (MQ=255) ccGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGc < 1:105178/251‑1 (MQ=255) cACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAgc > 1:115874/1‑251 (MQ=255) cACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCAGCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAgc < 1:253927/251‑1 (MQ=255) gcgcgcgAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCt < 1:183059/251‑1 (MQ=255) gcgcgAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTc < 1:304918/251‑1 (MQ=255) cgcgAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTc > 1:724428/1‑250 (MQ=255) cgAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGAAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCCGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGACTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCCGCGCTTCact > 2:365883/1‑249 (MQ=255) gAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGAGCTTCAATg > 2:731489/1‑251 (MQ=255) gAACTTCTTAAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGGGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACAGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATg > 2:144460/1‑251 (MQ=255) tcttcAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGtt < 1:91437/250‑1 (MQ=255) cttcAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTg > 1:443642/1‑251 (MQ=255) ttcAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGt > 1:526445/1‑251 (MQ=255) ttcAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGAGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGt > 2:151508/1‑251 (MQ=255) tcAAGGTTGGATTTCAGGTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGTCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCACTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGttt < 2:98285/248‑1 (MQ=255) aTGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTg < 1:707266/251‑1 (MQ=255) tGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGATTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAATTCTTCTGCCAGCGCTTCAATGCGTTTGTCCTgg > 2:442826/1‑250 (MQ=255) gTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGCTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGGGTTTCAGTTCGTCAGCAGCGTAGGCTTCATCGTGAATGTAAGAGATCTATTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTCCTGATCGCCACGGACCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTgag < 1:485055/251‑1 (MQ=255) ggATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGAc < 2:443642/250‑1 (MQ=255) | ATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCT‑TCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGAC > NC_000913/3911834‑3912322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |