Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 92,843 A→G P477P (CCA→CCG)  ftsI → peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091392,8430AG98.6% 230.0 / ‑4.1 71P477P (CCA→CCG) ftsIpeptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (39/31);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-01

GCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTAT  >  NC_000913/92608‑93084
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  
gctttaaaccTCAAAAACAACGGTGGTATGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTAGCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTg                                                                                                                                                                                                                                     <  2:584314/243‑1 (MQ=255)
gCTTATATCCTCAAAAACAACCGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCACAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGACTCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTg                                                                                                                                                                                                                                     >  1:851345/1‑251 (MQ=255)
   tatttCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTAGCGGGCGGCGGCGGCGTGaa                                                                                                                                                                                                                                   <  2:127651/246‑1 (MQ=255)
        ccTCAAAAACCAAGGTGGCCTGCCATAGAGAGTGCCACCTTCTCTTCCGACTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTAGATTACCAAAGTTAAACCCACGGGTCCCGGTGACCGAGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CCTATGTTGGCACGCGTGACGCTACCGGGCGGCGCCGGAGTGCAggcggc                                                                                                                                                                                                                             >  2:73225/1‑251 (MQ=255)
           cAAAAACAACGGTGGTCGGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGGTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGAt                                                                                                                                                                                                                          <  2:149414/251‑1 (MQ=255)
            aaaaaCAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGAtt                                                                                                                                                                                                                         <  1:503274/251‑1 (MQ=255)
              aaaCAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGGAGGCGGCGATTa                                                                                                                                                                                                                        >  2:364626/1‑250 (MQ=255)
              aaaCAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTaa                                                                                                                                                                                                                       <  2:649071/251‑1 (MQ=255)
              aaaCAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGGGAAGGCGGCGATTa                                                                                                                                                                                                                        >  1:351947/1‑250 (MQ=255)
                 caacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAgg                                                                                                                                                                                                                    >  1:657004/1‑251 (MQ=255)
                  aacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGc                                                                                                                                                                                                                   >  1:618188/1‑251 (MQ=255)
                   acGGGGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGAAACACAGTTACAGTTAGCGAGAGTCTCCGCAACTATCGGCAGCTACGGCATTTAACGCCCACTGTCGATTACAAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCGTTGTCCGACCCGTGGAG‑CACATGGTGGAAAGAGTGGGGCTACCGAGCGGCGGCGGCGGGAAGTGGGGGGTTAAAGGCt                                                                                                                                                                                                                  >  2:153829/1‑251 (MQ=255)
                       tggtCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcg                                                                                                                                                                                                              >  1:729156/1‑251 (MQ=255)
                       tggtCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcg                                                                                                                                                                                                              >  1:499693/1‑251 (MQ=255)
                        ggtCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcg                                                                                                                                                                                                              <  1:461323/250‑1 (MQ=255)
                            tGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtatc‑g                                                                                                                                                                                                        >  1:533047/1‑251 (MQ=255)
                             gACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtat                                                                                                                                                                                                           <  1:448122/248‑1 (MQ=255)
                                 aagagagGGCCCCCTTTCCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACTGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCAtt                                                                                                                                                                                                   <  2:618188/250‑1 (MQ=255)
                                   gagagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTa                                                                                                                                                                                                  >  1:167279/1‑250 (MQ=255)
                                    agagGGCCAACTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTGCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTaaa                                                                                                                                                                                                <  1:79128/251‑1 (MQ=255)
                                     atgggccgacttatctgtcgtcgaCGGGCTAAGGGTGACGCCGTTCCAGTTAGCGTGAGTCTACGCAACTTTCGGCTGCTACTGCATTTATCGCCCACTGTCGATTACCAAAGTTGTCCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCTGTGTCCACGCCGTGGTA‑CATATGATGGAAATCGTTGCGCTACCGGGCGGCGGAAGCGTGAAGGCGGCGATTACAGGCTAGCGTAGC‑TCCATTaaaa                                                                                                                                                                                               <  2:675989/228‑1 (MQ=255)
                                      agggccgcctgttctTTCGGCTACGTGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTTCGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAAc                                                                                                                                                                                              <  2:147392/239‑1 (MQ=255)
                                       gggCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGCACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGGGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTCAAAcc                                                                                                                                                                                             >  2:447424/1‑251 (MQ=255)
                                        ggCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGTCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCg                                                                                                                                                                                            >  1:235996/1‑251 (MQ=255)
                                            aCCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTAc                                                                                                                                                                                        >  1:815671/1‑251 (MQ=255)
                                              cTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTAACGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTAACGTATC‑GCCATTAAAACCGGTAc                                                                                                                                                                                        >  1:421229/1‑249 (MQ=255)
                                                  tctTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGa                                                                                                                                                                                   >  1:778154/1‑250 (MQ=255)
                                                       cGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAg                                                                                                                                                                              <  1:364626/250‑1 (MQ=255)
                                                         gCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTACCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTc                                                                                                                                                                           >  1:537830/1‑251 (MQ=255)
                                                           tACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGAAGCTACGGCATTTATCGCCCACTGTCGATTACCACAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGATACCGCGAAAAAGGTCgg                                                                                                                                                                         >  1:831218/1‑251 (MQ=255)
                                                             cGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTaaa                                                                                                                                                                                                                      <  2:714135/205‑1 (MQ=255)
                                                             cGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTaaa                                                                                                                                                                                                                      >  1:714135/1‑205 (MQ=255)
                                                                   aaTGGTAACACCGTTACAGTTAGCGCGAGTCTACGCGACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGAc                                                                                                                                                                  <  1:648208/250‑1 (MQ=255)
                                                                      ggTAACACCGTTACAGTTAGCGCGAGGCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCTCTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGt                                                                                                                                                               <  2:868767/250‑1 (MQ=255)
                                                                             ccgttACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACAt                                                                                                                                                       <  1:378726/248‑1 (MQ=255)
                                                                             ccgttACAGTTAGCGCGAGTCGACGCAACTTGCGGCATCTACGTCATTGATCTCCCACTGTCGATGACCAAAGTTGACCCCCCGGTTCCCGGTGGACGTGGCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGGGGCGGCGGCGGGAATGCGGCGATTAAAGGCGATCGGATC‑GCCATTAAGACCGGGGCCGCGAAAAATGGCGGTCCGGACGGTCGCTACAt                                                                                                                                                       <  2:808420/248‑1 (MQ=255)
                                                                                       tAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCTCCCACTGTCGATTACCAAAGTTGACCCCCCGGTGCCCGGTTAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAATGCTATCGTAAC‑GCCATTAAAACCGGTACCGCTAAAAAGTTCGGGCCGGACGGTCGCTACATCaataaata                                                                                                                                              <  1:410947/250‑1 (MQ=255)
                                                                                          cgcgAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAAAGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCCTTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGCAGGTCGGTACATCACTAAATATAtt                                                                                                                                          >  2:454704/1‑251 (MQ=255)
                                                                                           gcgAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATCTGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAAGAGGCGATTAAAGGCTGTCGTATC‑GGCATTAACACCGGTGCCGCGTAAAAGACCGGGCCGGACGGGCGCTCCATCAATAAATATATTg                                                                                                                                         >  2:659144/1‑251 (MQ=255)
                                                                                           gcgAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTg                                                                                                                                         <  1:695580/251‑1 (MQ=255)
                                                                                             taGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCCAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGACGGCGGCGAATAAAGGCTATCGTATCCGCC‑TTAAAACCGTTACCGCGAAACAAGTCGGGCCGGACGGTCGCTAACTCCATAAATGTAttcct                                                                                                                                       >  2:208561/2‑248 (MQ=255)
                                                                                             gAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCACACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGACCGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGGTGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTCACGGCTATCGTATC‑GCCATTAAACCCGGTACCGCGAAAAAGGTAGGGCCGGCAGGTCGCTACATCAATAATTATATTGCt                                                                                                                                       >  2:531624/1‑251 (MQ=255)
                                                                                               gTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCtt                                                                                                                                      >  1:827022/1‑250 (MQ=255)
                                                                                                             cGGCAGCTACACCATCTATCGCACACGCTCGCTTACCAAAGTAGAACCCCCGGTTCCCCGTGACCGTGTATTCCCGGAACCCATTGTCCGCGCCGTGATG‑CATATGATGGAAAGCGTGGCGCTACCGAGCGGCGGCGGCGGGAAGGCGGCGATTAAAGGCTATCGTATC‑ACCATACAAACCGGTACCGCGAAAAAGGTCGGGCCGGAAGGTAGCTACACCAATAACAATATTGCTTATACCGCAAGCGTTg                                                                                                                       >  2:119025/1‑251 (MQ=255)
                                                                                                                cAGCTACGGCCTTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTgcgc                                                                                                                    >  1:164683/1‑251 (MQ=255)
                                                                                                                cAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTgcgc                                                                                                                    >  1:578216/1‑251 (MQ=255)
                                                                                                                     tcGGCATTTATCGGCCCCTGTCGATTACCAAAGTGGTCCCCCCGGTTCCCGGGTAACGTGTCTTCCCGGAATCCATTGTCCGCCCCGTGGTG‑CATATGATGGAAAGCTTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGATAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGc                                                                                                                <  2:783143/249‑1 (MQ=255)
                                                                                                                      cGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTTAACGTGTCTTCCCGGAATCCATTGGCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGa                                                                                                              <  1:615461/251‑1 (MQ=255)
                                                                                                                         cATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑AATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTc                                                                                                           >  2:13906/1‑251 (MQ=255)
                                                                                                                              aTCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGTCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCcg                                                                                                      <  2:235996/251‑1 (MQ=255)
                                                                                                                              aTCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGAGTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCcg                                                                                                      <  1:545479/251‑1 (MQ=255)
                                                                                                                                          cGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGGCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCGACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCGTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCt                                                                                           <  2:698686/250‑1 (MQ=255)
                                                                                                                                           gATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTgg                                                                                         >  1:10902/1‑251 (MQ=255)
                                                                                                                                             ttACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGCAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGgtt                                                                                       >  2:262588/1‑251 (MQ=255)
                                                                                                                                             ttACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGAGCTGgtt                                                                                       >  2:568413/1‑251 (MQ=255)
                                                                                                                                                ccAAAGGTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCAGTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGgttgtt                                                                                    <  2:793502/251‑1 (MQ=255)
                                                                                                                                                        gACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCaa                                                                            <  1:170692/251‑1 (MQ=255)
                                                                                                                                                        gACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCa                                                                             <  1:865073/250‑1 (MQ=255)
                                                                                                                                                           cccccGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACg                                                                          >  1:616630/1‑250 (MQ=255)
                                                                                                                                                                    cccGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAgtc                                                                >  1:749850/1‑249 (MQ=255)
                                                                                                                                                                               tggcatCCCGGAATACATTGTACGCACCGTGGTG‑CATATGATGGAACGTGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGCATC‑GCCATTAACACCGGTACCGCGAAAAAGGTCGGGACGGCCGGTCGCTACTTAAATAAATATATTGCTTATACCGCCGGCGCTGCGCATGAGAGTCAGCCGCGCTTCGCGCGCGTTTATTATATCAACGTTCCGCTGGCGGGTacacact                                                     >  2:332791/6‑245 (MQ=255)
                                                                                                                                                                                        ggAATCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAATACTAaggcgggg                                            >  1:29612/1‑249 (MQ=255)
                                                                                                                                                                                           aTCCATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGCCGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAAAACTAcgggcgcaccc                                         >  2:690117/1‑243 (MQ=255)
                                                                                                                                                                                             cctttGTCCGCACCGTG‑TGTCATATGATGGAAAGCGTGGCGCTCCCGGGGGGCGGCGGCGTGAAGTCGGCGAGTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCTGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGt                                        <  2:778154/247‑1 (MQ=255)
                                                                                                                                                                                             ccATTGTCCGCACCGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAATACTACGGCGGCGCCggt                                       >  1:229783/1‑249 (MQ=255)
                                                                                                                                                                                                        accgtggtg‑CATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAATACAACAGCGGAGCCGttttcccgcccgg                            >  1:678070/4‑241 (MQ=255)
                                                                                                                                                                                                             ggtg‑CATATGAGGGAAAGCGTGGCGCTACCGGGCGGCGGCTGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGGCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCttt                        <  2:576681/250‑1 (MQ=255)
                                                                                                                                                                                                                        atgGAAAGCGTGGCGCTCCCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCcatcat             <  1:167928/251‑1 (MQ=255)
                                                                                                                                                                                                                        agggAAAGCGGTGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTAGCGTATC‑GCCATTAAAACCGGTACCGAGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCcatcat             <  2:84605/249‑1 (MQ=255)
                                                                                                                                                                                                                                cGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGAGCCGTTTCCCCGCCGGGCCTTTGTGGCCTCATGGggggcg     >  1:599203/1‑251 (MQ=255)
                                                                                                                                                                                                                                   ggCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTTTCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGTGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTAt  <  2:616630/251‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  
GCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTG‑CATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATC‑GCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTAT  >  NC_000913/92608‑93084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑