Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,357,202	C→T	intergenic (‑92/+221)	sapA ← / ← ymjA	putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,357,202	0	C	T	100.0%	199.6 / NA	64	intergenic (‑92/+221)	sapA/ymjA	putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/29);  total (35/29)

GGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCA  >  NC_000913/1356965‑1357445                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     gggTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCg                                                                                                                                                                                                                                         <  1:622711/251‑1 (MQ=255) gggTTAAAGGTGGTGACTGGCCCGCTGACGCAATAGACAAAACCGGTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACGCACAAGTCCAGCAATCACCAAAAGAGACGGAAATACCTGGCGCATAATATTAAGGGATTTTAAGTAAAGAGACTATCTTACTAATATTAAATGAAATTTTCCATTTACGATTGAGTGCAGGGGTAGTAATGTGGGCTGTGTGGTTGGTCg                                                                                                                                                                                                                                         >  2:680167/1‑251 (MQ=255)  ggTTAAAGGGGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCCGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGt                                                                                                                                                                                                                                        >  2:217394/1‑251 (MQ=255)        atgggttcaCTTGCCCGCTGACGCAATAGACAAAACCGCTGGCGCGGA‑ATGTAGCATGCGGGGGAGAGTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAAGCTCAAAAAGAGACGAGAATACCTGGCGCATAATATTAAGTGAGTGTATGTAAAGAGGCTATCTTACTAATATTTAATGACATGTTCCATTACCGGTTGTGTTCAGGGGACGTAATGAGTGCTGTGTGCTGGGTCGTTTAACa                                                                                                                                                                                                                                  <  2:344204/243‑1 (MQ=255)        aGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACa                                                                                                                                                                                                                                  <  1:345835/251‑1 (MQ=255)               gACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACg                                                                                                                                                                                                                           >  1:34461/1‑251 (MQ=255)                 cTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTc                                                                                                                                                                                                                         >  1:457214/1‑251 (MQ=255)                   tGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGCTTCCGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGGGACGTCGc                                                                                                                                                                                                                       >  2:242630/1‑251 (MQ=255)                           gACGCAATTGACAAAACCGCTGTCGTGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTg                                                                                                                                                                                                                 <  2:597353/249‑1 (MQ=255)                              gCAATAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCa                                                                                                                                                                                                             >  1:87116/1‑250 (MQ=255)                               cAATAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAAc                                                                                                                                                                                                           <  1:441308/251‑1 (MQ=255)                                aaTAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAAc                                                                                                                                                                                                           >  1:68263/1‑250 (MQ=255)                                   agaaaaaaCCGCTGTCACGGATAT‑CAGCTGGCGGGGGAGATTCAGGCGCGGCGATTGTCTGACC‑ACTCACAAGTCCATCAATCACCAAAAGAGACGATAATCCCTGGCGCATAATATTAAGGTATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTAAGGGGTCGTAATGAGGTCTGTGTTGTTGGTCGTGGAACAGGTGACGTCGCCATCTGTTCAACAtt                                                                                                                                                                                                        <  2:310338/246‑1 (MQ=255)                                   aGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTc                                                                                                                                                                                                       <  1:322359/251‑1 (MQ=255)                                      cAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCGGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCACAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTACTGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGt                                                                                                                                                                                                     >  1:394801/1‑250 (MQ=255)                                        aaaCCGCTGTAGCGGATAT‑CAGCATGCGGGGGAGAGTCAGGCACGGCGGTAGCCTGACCCATCCAAAAG‑ACAGCAATCACAAAAAGAGGAGAGAATACCTAGCGAAAAATATAAGGGGATTTTATGGAAAAAGGCTATCTTACTAATATTTAATGACATTTGCCATTACAGTTTGGGTTCAGAGGTAGTAAGGAGGGATGTGTGGGTGGTAGGTGAACAGGTGACATAGCCAGCTGTTCAACATTCGTAcc                                                                                                                                                                                                  >  2:665694/1‑251 (MQ=255)                                           ccGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTAATAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACCTTCGTACCCGt                                                                                                                                                                                               >  1:778021/1‑251 (MQ=255)                                                    cgGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTctct                                                                                                                                                                                      <  1:802789/251‑1 (MQ=255)                                                             aGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTctataactat                                                                                                                                                                              <  1:441858/251‑1 (MQ=255)                                                               cATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATACTATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGG‑TCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCACCATTCGTACCCGTGATTTCTCtataactataac                                                                                                                                                                           >  1:242976/1‑251 (MQ=255)                                                                   cGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGCTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTca                                                                                                                                                                        <  2:303665/251‑1 (MQ=255)                                                                       ggAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGa                                                                                                                                                                    <  1:229449/251‑1 (MQ=255)                                                                                            gCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGCTTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCag                                                                                                                                                 >  2:295971/1‑249 (MQ=255)                                                                                                  cc‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTAc                                                                                                                                         <  1:217394/251‑1 (MQ=255)                                                                                                               ccAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATACCTATAACTCACAGCACAACTTAGCGGGGAGCAGAGCGGTACGATCGTGAGaa                                                                                                                              >  2:90660/1‑250 (MQ=255)                                                                                                               ccAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGaa                                                                                                                              >  1:663322/1‑250 (MQ=255)                                                                                                                   cAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCAGCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCCCAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAAccc                                                                                                                          >  2:32212/1‑250 (MQ=255)                                                                                                                        cccAAAAGAGACGATAATACCTGGAGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGATCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGACATCTGATCAACATTCGTACCCGTGTTTTCTCTATAACTATACCTCACAGAACAACTTAGTGAGGAGCAGAGCGGTACGATCGTAAGAACACCATTaa                                                                                                                     >  2:627963/2‑250 (MQ=255)                                                                                                                          caaacctgaCGATAATACCTGACGCATCGTATTCAGTGATTTTATGAAAGGAGGCAATCTTACTAATATTTACTGAAATTTGCTATTACCGTATGTGTTCAGGGGGCGTAATGAGGGCTGTGTGGTTGGTCGCTGACCAGGTGTCGTCGCCATCTGTTCAACATTCGTACCCGTGTTTTCTCTGTCACAATAACTCGCAGAAAAACTTAGCGAGTAGCAGAGCGGTACGATCGTGCGCAACCCATTAAAGc                                                                                                                  >  2:35890/8‑251 (MQ=255)                                                                                                                               gagaCGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAAGATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCt                                                                                                             <  2:34461/251‑1 (MQ=255)                                                                                                                                agtcGATAATACCTGTCGCATAATATTAAGGGATTTTATGTAAAGACGCTATCTTACTAATATTTAACGACATTTGCCATTACCGTTTGTGTTCATGGGTCGTAATGAGGTCTGTGTGGTTGGTCGTTGAACATGTGACGTCGCCATCTGTTCAACAATCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGATCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTg                                                                                                            <  2:292316/248‑1 (MQ=255)                                                                                                                                  aCGATAATACCTGGCGCATAATATTAAGGGATTTTATGTACAGAGGCTCTCTTACTAATATTTAATGACAGTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTAAGATCGTGAGAAACCCATTAAAGCACGCTGCa                                                                                                          <  2:280224/251‑1 (MQ=255)                                                                                                                                       aataCCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGAGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCg                                                                                                     >  1:614309/1‑251 (MQ=255)                                                                                                                                           ccTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTc                                                                                                 <  1:72049/251‑1 (MQ=255)                                                                                                                                              ggCGCATAATATTAAGTGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCAtt                                                                                              <  1:1857/251‑1 (MQ=255)                                                                                                                                                            aGGGATTTTATGTAAAGAGGCTATATTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGAAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACTTTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGAAGAGCGGTACGATCGTGAGAAACCAATACAAGCAAGCTGCCGTGCGTGTCATTGTTTGCCAGATgcg                                                                                >  2:341732/1‑251 (MQ=255)                                                                                                                                                              tgATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATgcggc                                                                              >  2:330135/2‑251 (MQ=255)                                                                                                                                                              ggATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTAACGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGGTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAAAACATTAAAGCACGCTGCAATGCGTATCATTGTTAGCCAGATgcggc                                                                              >  1:844732/1‑251 (MQ=255)                                                                                                                                                               gATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATgcggcg                                                                             >  1:624073/1‑251 (MQ=255)                                                                                                                                                                   ttATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGa                                                                          >  1:648945/1‑250 (MQ=255)                                                                                                                                                                    tATGTAAAGAGGCTCTCTTACTAATATTTAATGCCATTTGCCATTAACGTTTGCGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCACCATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGATGAACAGAGCGGAACGATCGTGAGCAACCCATTAAAGCACGATACAGTGCGTGTCATTGTTAGCCAGATgcgccgagaa                                                                         >  2:822277/1‑243 (MQ=255)                                                                                                                                                                     aTGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGaccg                                                                       >  1:299133/1‑248 (MQ=255)                                                                                                                                                                       gTAAAGAGGCTATCTTACTAATATTTAATGACATCTGCCATGTCCGTTTGTGTTCAGGGTTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCGGTTCAACATTCGTACCCGTGATTTCTCCATAACTATAACTCACAGAACAACTTAGCGGGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCGCGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCt                                                                    <  1:35890/251‑1 (MQ=255)                                                                                                                                                                        tAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTAACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCtt                                                                   >  1:254571/1‑251 (MQ=255)                                                                                                                                                                            gagGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATc                                                               >  1:638422/1‑251 (MQ=255)                                                                                                                                                                                      tACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAAc                                                       <  1:540118/249‑1 (MQ=255)                                                                                                                                                                                       aCTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGAt                                                    >  1:28575/1‑251 (MQ=255)                                                                                                                                                                                        cTAATATTTAATGACATTTGCCATTACCGTTTGAGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAACGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGAt                                                    >  1:194403/1‑250 (MQ=255)                                                                                                                                                                                          aaTATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACt                                                 <  2:638422/251‑1 (MQ=255)                                                                                                                                                                                               ttaatgtaaTTTGCCATTTCCGTTTGTGTTCAGGGGTTGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACTTCGCCATCTGTTCAACATTCGTACCCTTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCTGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATTCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGAcc                                             <  2:478214/242‑1 (MQ=255)                                                                                                                                                                                               gtAATGACAGTTGCCATTACCGTTTGTGGTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGATGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAATGATACTGAcc                                             <  1:822277/249‑1 (MQ=255)                                                                                                                                                                                                 aaTGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGa                                           <  1:90660/250‑1 (MQ=255)                                                                                                                                                                                                  aTGATATTTGCCATTACCGGTTGTGTTCAGGGGACGTAATGAGGGCTGTGTGGTGGGTCGGTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTTCCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCgctc                                         <  2:673325/251‑4 (MQ=255)                                                                                                                                                                                                       aTTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑CCGCTTTATCCGGACAACGATACTGACCGATCGTCTg                                    >  1:386898/1‑251 (MQ=255)                                                                                                                                                                                                            cctttACTGTTTTTGTTCAGTGGTCGTAATGCGGGCTTTGTGTTTTGTCGTTGAACAGGGGACGTCGCCATCTGTTCAACATTCTTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTATGATCGTGAGAAACCCATTAAAGCACGCTGCAGTTCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAAt                                <  1:811779/247‑1 (MQ=255)                                                                                                                                                                                                                 aCCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAt                           <  1:295971/250‑1 (MQ=255)                                                                                                                                                                                                                    gTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGATCGTCTTCAATTAAAtcatt                       <  1:32212/251‑1 (MQ=255)                                                                                                                                                                                                                     tttGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtcatt                       >  1:822698/1‑250 (MQ=255)                                                                                                                                                                                                                        gtgtTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtcattactc                   <  1:330135/251‑1 (MQ=255)                                                                                                                                                                                                                          gtTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtcattactcat                 >  1:173515/1‑251 (MQ=255)                                                                                                                                                                                                                            tCAGGGGTCGTAATGACGGCTGTGTGATTGGTCGCTGCACAGGTAAAGACGCCAACTCTTCAACATCCGTACCCGTAATTTCTCTATAACTATAACTCACAGAACAAATTAGCGAGACGAAGAGCGGTACGATCGTGAGAAACCCATTAAAGCCCGCTGCAGTGCGCGTCATTGATAGCCAGCTGCGGCG‑GACAAGCTGTCTCCGGACAACGATAATGACCCATCGACTgaaaataataacttactcatt                >  2:414119/1‑230 (MQ=255)                                                                                                                                                                                                                                     gTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTg       >  1:696637/1‑250 (MQ=255)                                                                                                                                                                                                                                     gTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGa      <  1:237902/251‑1 (MQ=255)                                                                                                                                                                                                                                          gAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAACGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGCCCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCa  >  1:828716/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     GGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATAT‑CAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACC‑ACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGA‑ACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCA  >  NC_000913/1356965‑1357445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑