Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,337,390 C→T K180K (AAG→AAA)  adiY ← DNA‑binding transcriptional activator AdiY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,337,3900CT92.2% 156.1 / 2.9 52K180K (AAG→AAA) adiYDNA‑binding transcriptional activator AdiY
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base T (23/24);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

TTAAGGTTAAAAATCAGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACG  >  NC_000913/4337155‑4337633
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   
ttAAGGTTAAAAATCAGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTTATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTggg                                                                                                                                                                                                                                      >  1:148681/1‑251 (MQ=255)
  aaGGTTAAAAATCAGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCt                                                                                                                                                                                                                                    <  1:548587/251‑1 (MQ=255)
    ggTTAAAAATCAGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTaa                                                                                                                                                                                                                                  <  1:844359/251‑1 (MQ=255)
               aGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAaacaa                                                                                                                                                                                                                       <  1:433072/251‑1 (MQ=255)
                          cgatctcTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTATTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGAGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGACAGGTGGAGATTATTTGGATATAACTTGTGTTTTCGATTTTCAACTTTTTTTTTAAAAGACTTGGGCTAAGACTTAAACAATTGGCTACCAt                                                                                                                                                                                                            >  2:211984/4‑251 (MQ=255)
                                ctGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTa                                                                                                                                                                                                        >  1:795251/1‑249 (MQ=255)
                                       gACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCa                                                                                                                                                                                                <  1:157721/250‑1 (MQ=255)
                                            aCATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCttt                                                                                                                                                                                          <  2:795251/251‑1 (MQ=255)
                                                   gCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACGTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAat                                                                                                                                                                                    <  1:404196/250‑1 (MQ=255)
                                                     aGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATc                                                                                                                                                                                 <  1:391641/251‑1 (MQ=255)
                                                       cggcgTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGc                                                                                                                                                                               <  1:290362/251‑1 (MQ=255)
                                                          cgTCAGACCGTAGAAGTCTTTAAAGACATAAATAAAGTACGACTTACTGTTTGAGCCGCAGGACTGGGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTTCGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATATCTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATGCCAGTCTTTGTGAATATCGCttt                                                                                                                                                                            <  2:763200/251‑1 (MQ=255)
                                                            tCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAATTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTc                                                                                                                                                                           >  1:174178/1‑250 (MQ=255)
                                                                aCCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCaata                                                                                                                                                                       <  1:25838/250‑1 (MQ=255)
                                                                  cGTAGTAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTCGCCGCAGGACTGTGATACCTGAAAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGTGCTAAGACATAACCAACTGGCTACCATACTAAGATTCCAGTCTTTTTGAATATCGCTTTAaatactt                                                                                                                                                                    >  2:594728/1‑248 (MQ=255)
                                                                  cGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAAtt                                                                                                                                                                    <  1:103738/251‑1 (MQ=255)
                                                                         gTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTTAGAGATATTTTTACCATCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATaaa                                                                                                                                                             <  2:241832/251‑1 (MQ=255)
                                                                              tAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCt                                                                                                                                                         <  1:286243/250‑1 (MQ=255)
                                                                               aaaGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGt                                                                                                                                                       <  1:798855/251‑1 (MQ=255)
                                                                                aaGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTc                                                                                                                                                      >  1:19443/1‑251 (MQ=255)
                                                                                 aGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTTGCTTTAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATTCTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTTATAAACTCTGTcc                                                                                                                                                     >  2:409572/1‑250 (MQ=255)
                                                                                  gACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGCTATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAATAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATACTTTGTTAAACGTTGTCAc                                                                                                                                                    >  2:238535/1‑251 (MQ=255)
                                                                                      gAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATAACTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCTCTTTTCAACTTTTTTTTTAACAGACTTGGGCTACGACATAAACATATGGCTACCATTATAAGATTCCAGTCTTTGTGAATATCACTTTAAATAATTTTATAAACGCTTTCACTTac                                                                                                                                                >  2:618267/1‑251 (MQ=255)
                                                                                      gAAAAAAAGTACGACTTACTGTTGTAGCCGCAGGACTGTTATACCTGAGAGATATTTTTACCGTCCATCATTAATTTATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTTTCATACTAAGATTCCAGTCTTTGTGAATATCGCTTGCAATAATTTGATAAACGCTGTCACTTac                                                                                                                                                <  2:779671/251‑1 (MQ=255)
                                                                                       aaaTAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGAGACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTaca                                                                                                                                               <  2:19443/251‑1 (MQ=255)
                                                                                         aTAAAGTACGACGTACTGATGTAGCAGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGATTTCAATAATTTGATAAACGCTGTCACTTacac                                                                                                                                              >  1:150354/1‑250 (MQ=255)
                                                                                          tAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACTTGAGCGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGGGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAATTTTTTTTTTAACAGACTTGGGCTAAGACATAACCACCTGGCTAACATACTAAGATTCACGTCTTTGGCAGTTTCGCTTTCAATAAATTGAtcaaactattcacttaacaa                                                                                                                                             >  2:850098/1‑231 (MQ=255)
                                                                                            aaGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTGTACCGTCCATCATTAATTCATTTACGGCATAACGCATTCGGCAGGTGGTTATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAAtt                                                                                                                                          <  1:356655/251‑1 (MQ=255)
                                                                                              gTACGAAGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTAc                                                                                                                                        <  2:376416/251‑1 (MQ=255)
                                                                                               tACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACg                                                                                                                                       >  1:6894/1‑251 (MQ=255)
                                                                                                       aCTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACata                                                                                                                                <  1:200838/250‑1 (MQ=255)
                                                                                                          gttgtAGCCGCAGGACTGGGATACCTGAGAGATATTTTTACCGGCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACAtata                                                                                                                              <  1:526558/249‑1 (MQ=255)
                                                                                                                   gCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTTGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATg                                                                                                                   <  2:346646/251‑1 (MQ=255)
                                                                                                                                       agaTAGTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATTACTGGCGTTTTCGCTTTCCAACTTTTTTTTTAACAGACTTGCGCTAAGACATAAACTACTGGCTACCATACTAAGAGTCCAGTCTTTGTGAATATCGCTCGCAATAATTTGATAAACGCTGTCACGTACACAATTACGTAACATATACATCATTAATGAAACGAATATCTTACTGTCg                                                                                               <  1:831488/251‑1 (MQ=255)
                                                                                                                                         atatTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGGGATTATTTGGCTATAACTGGAGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGTCATAAACAACTGGCTACCAGACTAAGATTCCAGTCTTTATGAATATAGCTTTCACTAATTTGCTAAACGCTGTCACTTCCAAAATTACGTAACATATCCATCATTAATGAAACGAATTTTTTACTTTCg                                                                                               >  2:780703/1‑249 (MQ=255)
                                                                                                                                           atTTTTACCGTCACTCATTAATTCATTAACGGCATCACGCATGCGTCAGGTGGTGATTTTTTTGCTCTAACTGGTGATTTCGCTTTTCAACTTTTTTTTTAACAGATTTTGGCTAAGACATAATCAACTGGCTACCATACTAAGATTCAAGTCTTTGTGAATATCGCTTTCACTAATTTGATAACCGCTATCACTTACACAATTACGTACAATATACATCATTAATGAAACGAATTTTTTACTGTAgaga                                                                                            >  2:754225/1‑250 (MQ=255)
                                                                                                                                                tACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGAGGTGATTCTTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACgg                                                                                      >  1:733381/1‑251 (MQ=255)
                                                                                                                                                                      tACGGCATAACGCATGCGGCAGGGGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTACATAATTTGATAAACGCTGTACCTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTAATGTCGATAAACAGGGATAGACCCGTGaataataat                                                                 >  2:157629/1‑250 (MQ=255)
                                                                                                                                                                              aaCGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGCTAGCACCGTGAATAAAAATGCGCGTgtt                                                        >  2:70845/1‑251 (MQ=255)
                                                                                                                                                                                       ggCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGATAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTATTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGAATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCttt                                               >  1:402599/1‑251 (MQ=255)
                                                                                                                                                                                         cAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTc                                             >  1:395632/1‑251 (MQ=255)
                                                                                                                                                                                          aGGTGGAGGTTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCACTTTCAATAATTTGCTAAACGCAGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGAGCGTGTTCGtttcttttct                                            >  1:320050/1‑251 (MQ=255)
                                                                                                                                                                                                       ttGGCTATCACTGATATTTTCGCTTTTCAACTTTTTTTTTATCAGACTTGGGCTAAAACTTAAAAAACTTGCTACCTTGTTAAGATTCCAGTCTTTGTGAATATAGATTTCAATAATTTGATAAAAGCTGTCACTTACACAAATACGTAAAATATACATCATTAATGAAACGAATTTTTTACATCCGAGAAAACAGGAAATAACCGCGAATAATAATGATCGTGTTCGTACATTGTGTGACACAGTTTCt                                >  2:609899/1‑250 (MQ=255)
                                                                                                                                                                                                       ttGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATAGCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCt                                <  1:714125/250‑1 (MQ=255)
                                                                                                                                                                                                        tggagataACTGGTGTTTTCGCTTTTCATCTTATTTTTTAACAGACTTGGGCTAAGACATAAACACCTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAATCGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCta                               <  2:725199/245‑2 (MQ=255)
                                                                                                                                                                                                             ataACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGc                          >  1:53875/1‑250 (MQ=255)
                                                                                                                                                                                                              taACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGcat                        >  1:129116/1‑251 (MQ=255)
                                                                                                                                                                                                                        ttccGCTTTTAAACTTTTT‑TTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTgtgt              <  1:316245/247‑1 (MQ=255)
                                                                                                                                                                                                                         ttCGCTTTTCCACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGACTATCGCTTTCACTAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGCATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATACTAATGCGCGTGTTCGTTCCTTTTCTGCCTCAGTTTCTTCGGGCTTCCTGCTGTGtt             >  2:280849/1‑251 (MQ=255)
                                                                                                                                                                                                                               tttCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGcgcg        <  2:148681/250‑1 (MQ=255)
                                                                                                                                                                                                                                  cAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCaa    <  1:280849/251‑1 (MQ=255)
                                                                                                                                                                                                                                    aCTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAAcg  <  1:427626/251‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   
TTAAGGTTAAAAATCAGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACG  >  NC_000913/4337155‑4337633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑