Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	791,688	G→A	S123F (TCC→TTC)	galE ←	UDP‑glucose 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	791,688	0	G	A	97.6%	237.4 / ‑4.1	84	S123F (TCC→TTC)	galE	UDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (33/49);  total (34/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGATGTATGGCATCAGGTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGG  >  NC_000913/791441‑791932                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      ggCGATGTATGGCATCAGGTTTATCGGAATGCCTTGCGGATCTGCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTGTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGTTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGc                                                                                                                                                                                                                                                    <  2:5585/251‑1 (MQ=255)  gggatggatgGCATCAGGTTAGTCGGAATGCCTTGCGGATCTTCGCACATATCGCCAGACGGATGCGCGCCAACCGGGTTGAAGGAGCGCAGCAGGGCAATGCTCCAGGCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCt                                                                                                                                                                                                                                                   <  2:793245/249‑1 (MQ=255)     aTGTATGGCATCAGGTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTaaa                                                                                                                                                                                                                                                <  2:56787/251‑1 (MQ=255)       gTATGGCAGCAGGTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGAGTCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTGTTGCCGTAAGGGCGTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAAATTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTaaaa                                                                                                                                                                                                                                               <  2:486753/250‑1 (MQ=255)           ggCATCAGGCTATTCGGAATACCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCAAACCGGGATGAAGGAGCGCAGCAGGGCAATGCTCCCGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATAGTTTCCGCCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGGGGTCCGGACGAGGAGCTTTCAACGTGTGGACTTTTGGGCTGATCGCCATAAACGGGGGCCGCGAAGCTAAAAATa‑a                                                                                                                                                                                                                                          >  2:664383/1‑250 (MQ=255)              aTCAGGTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGtt                                                                                                                                                                                                                                      <  1:506768/251‑1 (MQ=255)                  ggTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTg                                                                                                                                                                                                                                  <  1:672701/251‑1 (MQ=255)                   gTTATTCGGATTGCCTTGCGGATCTTCGCCCATAACGCCCGACGGCTGCGCGCCAACCGGGTTGAAGTAGCTCAGCAGGTCATTGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCTTAAGGGCTTTGCGGTTTGCCGGTCGTTAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGTAGAAGCTAAAAATA‑AAGTTTTTg                                                                                                                                                                                                                                  <  1:521967/250‑1 (MQ=255)                         cGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTGCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTa                                                                                                                                                                                                                            <  1:468351/250‑1 (MQ=255)                         cGGAATGCCTTGCGGATCGTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTa                                                                                                                                                                                                                            <  1:94402/250‑1 (MQ=255)                          ggAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAgc                                                                                                                                                                                                                          >  1:580203/1‑251 (MQ=255)                                ccTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCCGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGCAGCTTTCAACGTATGGACTTTTGGGCTGATCGCCTTAAACGGAGGAGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAACGgcgcg                                                                                                                                                                                                                    >  2:75865/1‑251 (MQ=255)                                 cTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGCAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCCGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGCGTGCCGGTCGGGAAGCTTTCAAAGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAATTA‑AAGTTTTTGACGTTAGCGgcccgc                                                                                                                                                                                                                   >  2:765282/1‑251 (MQ=255)                                    gCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATATGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGCCGTTAGCGGCGCGCagg                                                                                                                                                                                                                >  1:99293/1‑249 (MQ=255)                                         tCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGGTTTTGACGTTAGCGGCGCGCATgtggct                                                                                                                                                                                                           >  2:402275/1‑246 (MQ=255)                                            tCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCt                                                                                                                                                                                                           <  1:708312/248‑1 (MQ=255)                                             cGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCTCGCATGGCGCTAAt                                                                                                                                                                                                        >  2:310909/1‑250 (MQ=255)                                                 cATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGTAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGAGGCGGAGAAACTAACAATA‑AAGTTTTTTACGTTAGCGTCGCGCATGGCGCTAATCAGgc                                                                                                                                                                                                   >  1:167657/1‑251 (MQ=255)                                                    atCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCa                                                                                                                                                                                                <  1:722642/251‑1 (MQ=255)                                                      cGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCTCCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCag                                                                                                                                                                                               <  1:303999/250‑1 (MQ=255)                                                       gCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGgcgaag                                                                                                                                                                                               >  2:141950/1‑246 (MQ=255)                                                         ccGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCCACGTCTGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTg                                                                                                                                                                                           >  1:151547/1‑251 (MQ=255)                                                                     cgcCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTCGCGGCGCGCATGGCGCTACTCAGGCGCAGAGTGCCGTTGACAttt                                                                                                                                                                               >  1:604597/1‑250 (MQ=255)                                                                     cgcCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACAttg                                                                                                                                                                               <  2:123348/251‑1 (MQ=255)                                                                      gcCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACAttgt                                                                                                                                                                              <  2:121982/251‑1 (MQ=255)                                                                       ccAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGGTCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACAttgtt                                                                                                                                                                             <  2:793585/251‑1 (MQ=255)                                                                            cgtgTTGCAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGAAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGCGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTCAACATC‑AAGTTTTTGACGTTAGAGGCGGGCATGGGCCTAAACCAGCGCAGGGTGCCGTTGTCAatgttggctg                                                                                                                                                                        >  2:289136/4‑247 (MQ=255)                                                                              ggTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGtc                                                                                                                                                                       >  1:378452/1‑249 (MQ=255)                                                                               gTTGAAGTAGCGCAGCAGGGCAATGCTCCTGTCCGGCTGGGCTTTTTGCAGATCAGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGACGCTTTCAACGTATGGCATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTACAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGAGCTAATCAGGAGCAGAGTGCCGTTGCCATTGTTGTCGTAat                                                                                                                                                                     >  1:314406/1‑251 (MQ=255)                                                                                ttGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAat                                                                                                                                                                     >  2:508417/1‑250 (MQ=255)                                                                                     gTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTcc                                                                                                                                                                >  1:30973/1‑250 (MQ=255)                                                                                     gTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCa                                                                                                                                                               <  1:424499/251‑1 (MQ=255)                                                                                          gcagcaGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGt                                                                                                                                                          >  1:210975/1‑251 (MQ=255)                                                                                               agGGCAATGCTCCAGTCCGGCTGGGGTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTg                                                                                                                                                     <  2:174356/251‑1 (MQ=255)                                                                                                    aaTGCTCAAGTCCGGCTGGACTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTAGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATAAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCTGCGGTGTTTGTGCCg                                                                                                                                                >  2:165996/1‑251 (MQ=255)                                                                                                       gCTCCAGTTCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGAt                                                                                                                                              <  1:373887/250‑1 (MQ=255)                                                                                                       gCTCCAGTCCTGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGAt                                                                                                                                              <  1:280185/250‑1 (MQ=255)                                                                                                       gCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGAtt                                                                                                                                             <  1:300384/251‑1 (MQ=255)                                                                                                                 ggCTGGGCTTTTTGCAGATCGGTGATGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGCAGCTTTCAACGCATGGCATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGAAGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGAGGTTTTTGTACCGATTCGCCCACGGc                                                                                                                                   >  2:500891/1‑251 (MQ=255)                                                                                                                 ggCTGGGCTTTTTGCAGATCGGTGAGGATCTGGTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTGGACATTGTTGTCGTAATATTCCAGCGGTTTTTGGACCGATTCGCCCACGGc                                                                                                                                   <  2:127722/251‑1 (MQ=255)                                                                                                                     gggCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTc                                                                                                                               >  1:298009/1‑251 (MQ=255)                                                                                                                      gggtttttGCAGCTCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGGCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCa                                                                                                                              <  2:132152/248‑1 (MQ=255)                                                                                                                             tGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGc                                                                                                                       >  1:208861/1‑251 (MQ=255)                                                                                                                                 gATCGGGGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAg                                                                                                                   <  2:673496/251‑1 (MQ=255)                                                                                                                                     ggTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGa                                                                                                               <  2:552409/251‑1 (MQ=255)                                                                                                                                         atgatgtGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGGCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCAc                                                                                                           <  2:207627/245‑1 (MQ=255)                                                                                                                                               tGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGATAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGGGTc                                                                                                     >  1:787418/1‑251 (MQ=255)                                                                                                                                                gTTCCCCCAGCAGCTTGCGTGTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCGTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCTATTCGCCCACTGCTTTCAGCCCGGCGAAGTGGATCACGGTGTc                                                                                                     <  2:740389/250‑1 (MQ=255)                                                                                                                                                   cctacagtagattgattttTCCGTAAGGGTTTTGCGGTGTGCAGGTCGGGAAGCTTTCAACGTATGGAAGTTTGGGCGGATCGCCATAATAGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAAGCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTTCAGCGGTTTTTGTACCGATGCGCCCACGGCTGTCAGCCCGGCGAAGTGGATCACGGTGTCGATa                                                                                                 <  2:503812/236‑1 (MQ=255)                                                                                                                                                   ccaccaTCAGCTTGCTTTTGGCGTAAGGGCTTTGCGGTTTGTCGGTCGGGAAGCTTTCAACGTTTGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGCTCACGGTGTCGAt                                                                                                  <  2:460511/250‑1 (MQ=255)                                                                                                                                                     cccaTCAGCTTGCTTTGGCCGGAAGGGCTTTGCGGTGTGCCGGTCGGGATGCTTTCAACGTCTGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGTTGTCGATAGc                                                                                               <  2:283287/250‑1 (MQ=255)                                                                                                                                                     accaTCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGc                                                                                               <  1:500891/251‑1 (MQ=255)                                                                                                                                                     accaTCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGACCGGCGAAGAGGATCACGGTGTCGATAGc                                                                                               >  2:55595/1‑251 (MQ=255)                                                                                                                                                       caTCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGGGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGt                                                                                             <  1:1918/251‑1 (MQ=255)                                                                                                                                                            gcttgcttTTGCCGTAAGGGCTTTGCGGGGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGGTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGCAGTGGATCACGGTGTCGATAGCGTGATCg                                                                                        >  1:281364/1‑251 (MQ=255)                                                                                                                                                               tgcttTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATc                                                                                         <  2:580203/247‑1 (MQ=255)                                                                                                                                                                      gccggtaGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAAGTTTGGGCTGAGCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTGTTGACGTTAGCGGCGCGCATGGCGCTAAGCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGAtct                                                                              <  2:30973/245‑1 (MQ=255)                                                                                                                                                                           aaGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGt                                                                          <  1:76180/250‑1 (MQ=255)                                                                                                                                                                               gCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGttatc                                                                      >  1:577951/1‑250 (MQ=255)                                                                                                                                                                                     cGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCtt                                                               <  2:210975/251‑1 (MQ=255)                                                                                                                                                                                         gtgCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGtt                                                           >  2:216463/1‑251 (MQ=255)                                                                                                                                                                                           gtcgggcgggaagtgttcatagtagggAATTTTAGGCTGCTTGTCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTGGTCGTTAGCGGCGCGCATGGCGCTACTCAGGTTCATAGTCCTGTCGACATTGTTGTCGTAATATTCCATCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGAGCGCGGTGTTGCTAGCGTGATCGTGTAGGATCTCGTTCATTAACGCCTCGTTAc                                                         <  2:811487/226‑1 (MQ=255)                                                                                                                                                                                                  gggAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATc                                                  >  1:823822/1‑251 (MQ=255)                                                                                                                                                                                                   ggAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAACCGGTGGCGGAGAAGTTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCGTCGTTACGAATATCg                                                 <  2:624856/251‑1 (MQ=255)                                                                                                                                                                                                    gAAGCTTTCAACGTATGGAATTTTGGGATGATCGCCATAAACGGGGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAACCCGGCGCAGTGGCTCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTAAGAATATCGc                                                >  1:615702/1‑251 (MQ=255)                                                                                                                                                                                                       gCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGTGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCtt                                             <  1:664383/251‑1 (MQ=255)                                                                                                                                                                                                       gCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCtt                                             <  1:141950/251‑1 (MQ=255)                                                                                                                                                                                                                 tATGGAATTTTGGTCTGATCGCCATAAACGGGGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACg                                    <  2:823822/250‑1 (MQ=255)                                                                                                                                                                                                                  aTGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGtt                                  <  1:508417/251‑1 (MQ=255)                                                                                                                                                                                                                     gAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGCTCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTggg                               >  2:772988/1‑250 (MQ=255)                                                                                                                                                                                                                      aaTTGTGGGGTGATCGCCAATAACGGTGGAGGCGAAGCTAAAAATATAGGTTTTTG‑CGTTAGCGGCGCGCATGGCGTTAACCAGGTGCAGAGTGCCGTTTACATTGTTGGCGTAATAGTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCTCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCTTGCAGGACCTCGGTCATCAACGCTTCGTTACGAATATCGTCTTCAACATACTTTggtt                              <  2:279267/251‑3 (MQ=255)                                                                                                                                                                                                                           tGGGCTGATCGCCAGAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACTTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGttt                          <  2:868417/250‑1 (MQ=255)                                                                                                                                                                                                                            gggCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTGTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTg                         <  1:765282/250‑1 (MQ=255)                                                                                                                                                                                                                            gggCTGAGCGCCATAAACGGTGGCGGAGAAGCTAAAAAGC‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTg                         <  2:295595/250‑1 (MQ=255)                                                                                                                                                                                                                              gCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGAGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCCGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACaaa                                      >  2:25518/1‑235 (MQ=255)                                                                                                                                                                                                                              gCTGATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGAGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACaaa                                      <  1:25518/235‑1 (MQ=255)                                                                                                                                                                                                                                 gATCGCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCt                   <  1:141522/251‑1 (MQ=255)                                                                                                                                                                                                                                    cGCCATAAACGGGGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGAGTCGATAGCGTGATCGTGCAGGATCTCGGTCCTCAACGCTTCGTTACGAATATCGGCTTCAACAAACGTTGGATGTTTGCCGCCTaa                 >  1:147827/1‑250 (MQ=255)                                                                                                                                                                                                                                     gCCATAAACGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGGTGGATGTTTGCCGCCTAAAc               >  2:865000/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTAAACGGTGGCGGCGAAGCTAAAAATA‑AAGCTTTTGACGTTAGCGGCGCGCATGGAGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGtt                                                           >  2:843309/1‑204 (MQ=255)                                                                                                                                                                                                                                        aTAAACGGTGGCGGAGAAGCTAAAAATA‑AAGCTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGtt                                                           <  1:843309/204‑1 (MQ=255)                                                                                                                                                                                                                                             cGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATa       <  1:455050/251‑1 (MQ=255)                                                                                                                                                                                                                                             cGGTGGCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATa       <  1:224832/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gCGGAGAAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGAGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAAAAgg  >  2:471173/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      GGCGATGTATGGCATCAGGTTATTCGGAATGCCTTGCGGATCTTCGCCCATATCGCCCGACGGATGCGCGCCAACCGGGTTGAAGTAGCGCAGCAGGGCAATGCTCCAGTCCGGCTGGGCTTTTTGCAGATCGGTGAGGATCTGTTCCACCATCAGCTTGCTTTTGCCGTAAGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATA‑AAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATATCGCCTTCAACAAACGTTGGATGTTTGCCGCCTAAACGCTCGATAACAGG  >  NC_000913/791441‑791932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑