Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,839	T→C	L12P (CTC→CCC)	pabB →	aminodeoxychorismate synthase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,839	0	T	C	100.0%	221.0 / NA	60	L12P (CTC→CCC)	pabB	aminodeoxychorismate synthase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/29);  total (31/29)

GAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGC  >  NC_000913/1894595‑1895073                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           gAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCg                                                                                                                                                                                                                                      <  1:393325/251‑1 (MQ=255)      gTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAgg                                                                                                                                                                                                                                 >  1:110105/1‑251 (MQ=255)                aGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGCCTGATGAAGACGTTATCTCCCGCTGGGATTACTTTACCCTGGCGTCAGGACGCCGCTg                                                                                                                                                                                                                        >  2:53899/1‑250 (MQ=255)                         gCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTAt                                                                                                                                                                                                                <  1:861111/249‑1 (MQ=255)                              caGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAAGTTTATTTCTccc                                                                                                                                                                                                         <  1:640560/251‑1 (MQ=255)                                gcgcGATTGCCTGTCCGCTGCTCATCCCTTGTGCCAACAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTATCTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCTAGTAGAGTCAGGACTGATGAAGCCGTTAGCTCCCGCTTTTATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTGATTTCTCCCGc                                                                                                                                                                                                       <  2:639250/251‑1 (MQ=255)                                    aaatGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGTTCCGCGCGACAGCTTTTTGCTGCTGTTCGTGGGTGAGTGAAGGTAAACCTGCAAAACTTGTTAACTCCTGCTAACTTGTTGGCGATAATTATTTCATGCTACACGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGTCGTCAGCACGCCGCTGAAATTTATTTCTCACGCTTa                                                                                                                                                                                                    >  2:766390/4‑250 (MQ=255)                                      ttGCCTGGACGCTGCTCATACATTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGc                                                                                                                                                                                                 >  2:111634/1‑251 (MQ=255)                                                     tCATCCCTTGTGCCGTCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGGTCATGGGTGCGTGACTGTAAACCTGCAAACTTTGTTAACTCCTGCTAACTTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGAAGTTATCTCCCGCTGTGATTACTTTCCCCTGGCGTCAGGACGCCGCTGAATTTTCTTTCTCCCACTTAATCCACCTGCCgacgtcc                                                                                                                                                                                  >  2:723679/1‑245 (MQ=255)                                                       aTCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACGCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGAt                                                                                                                                                                                <  1:711214/251‑1 (MQ=255)                                                         cccTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGc                                                                                                                                                                              <  2:302941/251‑1 (MQ=255)                                                         cccTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGc                                                                                                                                                                              >  1:846741/1‑251 (MQ=255)                                                           cTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCtt                                                                                                                                                                            >  1:328107/1‑251 (MQ=255)                                                                gCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTaca                                                                                                                                                                       <  1:305660/251‑1 (MQ=255)                                                                 ccatcggtctcTGGTTCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAACCCTGTAATCTTTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGACACATAGCCAGTATAGTCAGGACTGATGAAGATGTTATCGCCCTCTGTGATTACTTTACCCTGGCGTCAGGACGCCGTTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTacac                                                                                                                                                                      <  1:848747/241‑1 (MQ=255)                                                                 ccATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTacac                                                                                                                                                                      >  2:689330/1‑251 (MQ=255)                                                                            tGGATCCGCTAGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGGGATTACTTTACCCTGGCGACAGGCCGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCttttcaaccccgactatgc                                                                                                                                                           >  2:871538/1‑236 (MQ=255)                                                                              gATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCg                                                                                                                                                         >  2:136876/1‑251 (MQ=255)                                                                               aTCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTACGCCACCTGCCGTGGGCGATGCTTTTCAACTCCGGCTATGCCg                                                                                                                                                         >  2:277817/1‑250 (MQ=255)                                                                               aTCCGCACGACAGCTTTTTGCTGCTGTTCATAGGTGAGTGAAGGTAAACCCGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCAACCCGACACATAGCCAGTAGAGTCAGGACTGTTGCAGACGTTTTCTCCCGCTGGGATTAATTTACCCAGGCGGCATGACGCCGCTGACTTTTATTTCTCCCGCTTAAACCACCTCCCTGGGGCGATGCTTTTAAACTCCGGCTCTGCCg                                                                                                                                                         >  2:521926/1‑250 (MQ=255)                                                                                 ccGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGatc                                                                                                                                                      <  1:157762/251‑1 (MQ=255)                                                                                      cgcaaGCTTTTTGCTTCTGTTCAGGAGTGAGTGAAGGTAAAACTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCTCATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTTACGTGGGCGATGCTTTTACACTCCGGCTATGCCGatcatc                                                                                                                                                   <  2:586209/245‑1 (MQ=255)                                                                                       gACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGt                                                                                                                                                >  1:267047/1‑251 (MQ=255)                                                                                        aCAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGt                                                                                                                                                >  1:542273/1‑250 (MQ=255)                                                                                        aCAGCTTTTTGATGCTGTTCATGGGGGAGTGAAGGTAAACCTGCAAACATTGTTACCTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGAGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACCCTCCGGCTATGCCGATCATCCGt                                                                                                                                                >  2:444316/1‑250 (MQ=255)                                                                                            cTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAACCCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGCCGACGTTATCTCCCGCTGTGCTTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGc                                                                                                                                           >  1:759869/1‑251 (MQ=255)                                                                                              ttttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCg                                                                                                                                         >  1:447651/1‑251 (MQ=255)                                                                                                     gctgTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGa                                                                                                                                   <  1:722825/250‑1 (MQ=255)                                                                                                      ctgTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGat                                                                                                                                  >  1:3404/1‑250 (MQ=255)                                                                                                        ggtCATGGGGGAGTGAGGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATGTCATGCTACCGGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGGTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATGTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGatat                                                                                                                                <  1:357460/248‑1 (MQ=255)                                                                                                              gggTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTTCTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTc                                                                                                                         <  1:370683/251‑1 (MQ=255)                                                                                                                gtgagtgaAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCTGCACATAGTCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTtctc                                                                                                                                                                                                           <  1:519113/167‑1 (MQ=255)                                                                                                                gtgagtgaAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCTGCACATAGTCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGACGCTGGATTTTATTtctc                                                                                                                                                                                                           >  2:519113/1‑167 (MQ=255)                                                                                                                     gtaaGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACTTAGCCAGTAGAGTCCGGACTGAGGAAGACGTTATCTCCCGCTGTGTTTACTTTACCCTGGCGTCAGGAGGCCGCTGTATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCgccgagc                                                                                                                  <  2:402981/249‑1 (MQ=255)                                                                                                                       taGGTAAACATGCAACCATTGTTAACTCCTTCTAACTTATCGGCGATAATTATATCAAGCAACCAGGCACATAGCCAGTAGAGTCCGGACTGATGAAGACGTTATCTCCCGATGGGCATACTTTACCCTGGCGTCAGTACGCCGCTGACTTTTATTTCTCCCGCCTCAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCTGATAACCGCTTCGCTATTGTggactcggaaccc                                                                                                                >  2:299896/2‑240 (MQ=255)                                                                                                                       aaGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCgccgagccg                                                                                                                >  1:285568/1‑251 (MQ=255)                                                                                                                           tAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGAttt                                                                                                            >  2:173118/1‑251 (MQ=255)                                                                                                                              aCCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCa                                                                                                         <  1:697183/251‑1 (MQ=255)                                                                                                                              aCCTGCAAACATGTTTAACTCCTGCAAAATGTTTGGCGCAAATTATTTCAGGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCa                                                                                                         <  2:267047/251‑1 (MQ=255)                                                                                                                               ccTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGTTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGACTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAc                                                                                                        <  1:871538/251‑1 (MQ=255)                                                                                                                                  gCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACttt                                                                                                     >  1:352539/1‑251 (MQ=255)                                                                                                                                    aaaCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTaa                                                                                                   <  1:776954/251‑1 (MQ=255)                                                                                                                                         ttGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTAAGGACGCCGCTGCATTTTATTTCTCCCGCTTAAGCAACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATACGTATAGCCGCTTTGATATTGGGGTCGCCGAGCCGATTTGCACTTTAACCACt                                                                                              >  2:268457/1‑251 (MQ=255)                                                                                                                                          ttctAACTCCGGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGGGATTCCTTTACCCTGGCGGCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTcc                                                                                                                                                                   <  2:413955/178‑1 (MQ=255)                                                                                                                                          tGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTcc                                                                                                                                                                   >  1:413955/1‑181 (MQ=255)                                                                                                                                                 cccTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTaa                                                                                      <  1:682521/250‑1 (MQ=255)                                                                                                                                                     gCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAg                                                                                    <  1:277817/249‑1 (MQ=255)                                                                                                                                                        aaaTTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGc                                                                                                                                                                                   >  1:173869/1‑151 (MQ=255)                                                                                                                                                        aaaTTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGc                                                                                                                                                                                   <  2:173869/151‑1 (MQ=255)                                                                                                                                                              ttgGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAACGAAACCgatgtt                                                                         >  2:48044/1‑251 (MQ=255)                                                                                                                                                                ggCGCTAATTATTTCAGGCATCCCGGCACATGGCCAGTAGAGTAAGGCCGTATGAAGCCGTTACCCCCCGTTGTGTTTCCTTTACCCTGGCGCCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACTCTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTa                                                                        <  2:285568/250‑1 (MQ=255)                                                                                                                                                                 gcgcTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGt                                                                      >  1:818152/1‑251 (MQ=255)                                                                                                                                                                                 gCTACCCGTCCCATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGca                                                      <  1:745475/251‑1 (MQ=255)                                                                                                                                                                                    ccccGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGcaca                                                    <  1:689330/249‑1 (MQ=255)                                                                                                                                                                                    aCCCGACACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGACACCTGCCGTGGGCGCTGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGCAACCGTTGTTAGTGAAAGCGAAAAACGcaca                                                    >  1:552188/1‑250 (MQ=255)                                                                                                                                                                                                  cacgagagTCAGGACTTATGAAGACGTTATCTCCCGCTGTGATTACTTTCCCCTGGCGTCATGACGCCGCTTAATTTTATTTCTCCCTCTTACGCCCCCTGCCGTTGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCTCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAATAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGAc                                     <  1:58976/247‑1 (MQ=255)                                                                                                                                                                                                   aGTAGATTAAGGAATGATGAAGACGTTATCCCCCCCTTTGTTTTCTTCTCCCTGGAGTCGGGACGCCGCTAAATTTTATTTCTCCCGTTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATTGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCAAAACGACCACTGATGAcc                                    <  2:656758/251‑1 (MQ=255)                                                                                                                                                                                                                             tATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCt           <  1:173118/250‑1 (MQ=255)                                                                                                                                                                                                                              aTCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTgg         >  1:92474/1‑251 (MQ=255)                                                                                                                                                                                                                                       tgtgAT‑ACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATACTGTTACCCTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCCCCGGGCCGATTTGCACTTTAACCACTTTCGGTAGAGAAACAGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAAGACCTCCACCAGGTGCTGGAgcgcca  >  2:247903/1‑246 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           GAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGC  >  NC_000913/1894595‑1895073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑