Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,306,7360TG96.2% 148.9 / ‑5.8 53S325A (TCC→GCC) oppFmurein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (19/31);  minor base C (2/0);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCC  >  NC_000913/1306492‑1306977
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
ggTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGCACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑ACACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGCGTGCGCCAAAACAC‑GTCCTGTTCTGGAAGGGGGTTTCAGACCCGCCGttt                                                                                                                                                                                                                                              >  2:699524/1‑251 (MQ=255)
 gTAGAACCGGGGCCCTAGGATGAGGCCTCCCACAATCCACTACACCCTTACCCCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGTTTCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTCCCGTCGCCGAT‑CACCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTc                                                                                                                                                                                                                                             <  2:722572/251‑1 (MQ=255)
  tAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACCCCAGGGCATTGATGTCGGCAGACCCCATACCAGATCCGGCTCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACACGACTTACGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCAGAAACAC‑GTCATGTTCTGGAGGGGAGTTTCATACAGCCCGTTTCt                                                                                                                                                                                                                                            >  2:528092/1‑251 (MQ=255)
       cgggggACCTAGGATGAGGTCATCCACAACCCACTACCTCCTTACACCAGGGCATTGATGTCGGCAGCCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCt                                                                                                                                                                                                                                       <  2:206329/249‑1 (MQ=255)
        tGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGCGGGGAGTTTCAGACACGCCGTTTCTTGCCTg                                                                                                                                                                                                                                      >  2:182750/1‑251 (MQ=255)
         ggggACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGACGTTTCTTGCCTg                                                                                                                                                                                                                                      >  1:606421/1‑250 (MQ=255)
           ggcccTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGaaa                                                                                                                                                                                                                                   <  1:565320/248‑1 (MQ=255)
           ggACCTATGATGAGGTCTACCCCAATCCACTACAGCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGTTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGaaa                                                                                                                                                                                                                                   <  1:281914/251‑1 (MQ=255)
             aCCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGt                                                                                                                                                                                                                                 >  1:477451/1‑251 (MQ=255)
                catgttgtgggttACCACAATCCCCTACAGCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGTTCTGGATCGTGAGAAGATAAAAACCATCCATTTACTGTAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTGCCGGTTGTGTTTTCCGTTCCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAT‑GTCATGTTCTGGAGGGGAGTTTCAGACACGCCGCTTCTTGCCTTAAAGt                                                                                                                                                                                                                                 <  2:511444/236‑1 (MQ=255)
                aatgatgAGGTTTACCACAACCCACTACATCCTTACACAATGGCATGGATGTCGGCAGTCCCCATACCTGATCCGGCTCTGGAGAAGAACAAACCCATCCAGTTACTGGAAGGGGAACTACCGTAGC‑GTTACAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGa                                                                                                                                                                                                                              <  2:341794/250‑1 (MQ=255)
                 agtatggggTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCGGATCCGGATCGGGAGAAAAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGTTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTTCCTGAAAGTCGAt                                                                                                                                                                                                                             <  2:854200/244‑1 (MQ=255)
                     tgaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCGGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCACAACAC‑GTCCTGTTCTGGCGGGGAGTTTCAGAAACACCGTTTCTTGCCTTAAAGTCGATCCGc                                                                                                                                                                                                                         >  2:279140/1‑251 (MQ=255)
                      gaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATTCCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCTTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCt                                                                                                                                                                                                                        <  1:94551/251‑1 (MQ=255)
                                     ccACTACATCCTTACACCAGGGCAGTGTTGTCGGCAGTCCCCATACCTGATCCGGATCGGGAGTAGATCAAAACCATCCAGTTACTGGAATGGGAATTACCGTCGCGGAT‑CAACCCGCCTTCCGGTTTGGTTTTCCGTACCCGTGGCCTGTGTGCCGGTTTAGAGTGCGCTTAAACAC‑GTCCTGTTCTGGAGTGTAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCTATCCGCTTTAAAAACAATAAg                                                                                                                                                                                                          <  1:795957/250‑1 (MQ=255)
                                                 tcccccAGGGCAATGATCTCGGAATCCCCCAAACGTGTTCCGGATCTGGAGAAGAGCAAAACCCTCTAGTTACTGGAAGGGGAATTACCGGCGCCGAT‑CAGCCCGCCTCGCGGTTGTTCTTACCGTACCCTTCGCCCTATTGCCGGTCCAGAGTGCGCCAAAACTC‑GTCCTGTTCTGGAGGGGATTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATCAGGGCTGTCA‑Gtt                                                                                                                                                                                              <  2:226383/245‑1 (MQ=255)
                                                 tACACCAGGGCATTGATGTCGGCAGTCCCCCTACCTGACCCGGAGCTGGAGACGCACAAAACCATCCAGTTACTGGCAGGGGAATTACCGTGGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCAGTACCCGTTTCCCTATTGCCGGTCCAGAGTGCGCCAAAACAC‑GGCCTGTTATGGAGGGTAGTTTAAGACAACCCGATTCATGCCTGAACGTCGCGCCTCTTTCAAAACAACAAGGTCTAACA‑TTTGt                                                                                                                                                                                            >  2:517462/1‑251 (MQ=255)
                                                  accccAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAACCCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTc                                                                                                                                                                                           <  1:182750/248‑1 (MQ=255)
                                                         ggCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCtt                                                                                                                                                                                    >  1:796155/1‑251 (MQ=255)
                                                         ggCATTGATGTCGGCAGTCCCCATACCTGAACCGGATCTGTAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCATAT‑CAACCCTCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑TTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCtt                                                                                                                                                                                    <  1:413241/251‑1 (MQ=255)
                                                               gATGTCGGCATTCCCCATACCAGCTTCGGATCCGGAGAAGAACAAAACACTCCAGTTACTGGAAGGTGAATTCCCGTCGCCGTT‑CAACCCGCCTTCCGGTTGTGTTTCCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTTCGCCATAACTC‑TGCCTGTTTTTGAGGGTAGTTTCATACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAAGAAGGGCTTACA‑GTTGTCAGCTCTTTTTCAc                                                                                                                                                                              <  2:842238/251‑1 (MQ=255)
                                                                  gTCGGCAGTCCCCATACCTGATCCGGATCTGAAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTAACGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTACAGAAGGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTG‑CACGTTGGAAGCCCTTTTTccagct                                                                                                                                                                           >  2:625407/1‑246 (MQ=255)
                                                                        aGTCCCCATCCCTGATCCGGATCTGGAGAAGAACAACACCCTCCCGGTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGGTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGGGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAATATCAATAAGAGCTGACC‑GTTGTCAGCCCGTTTTAACGCTAAAAGc                                                                                                                                                                     >  2:39661/1‑251 (MQ=255)
                                                                               ataccgatgcgggATCTGGAGATAAAAAAAACCACCCAGTGACTGGAAGGGGAATTCCCGTCGCCGTT‑CAACCCGCCTTCCGGTTGGGTTGTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAATACAC‑GTCCTGTTCTGGAGGGGATTTCCAGACACGTCGTTTCTTGCCTGAAAGTCGATCCTCTGTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGattatg                                                                                                                                                              <  2:80446/240‑2 (MQ=255)
                                                                                 cccTGATCCGGATCGGGAGAAGAAAAAAACCATCCCGGTCCGGGAAGGGGAATTCCCGTCGCCGAT‑CAACCCGCCTGCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGAttattta                                                                                                                                                            <  2:41564/250‑1 (MQ=255)
                                                                                 acctattCCGGTTCTGGAGAAGAACAAAACCATCCTGTTACGGGAAGTGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGTTTGCCGTTCCAGAGTGCGCCAAAACAC‑GTCCTTTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGAttattt                                                                                                                                                             <  2:455107/244‑1 (MQ=255)
                                                                                  ccTGAGCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAAGTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTTCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGAttattta                                                                                                                                                            <  1:623577/250‑1 (MQ=255)
                                                                                     gATCCGGATCTGGAGACGAACAAAACCACCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTGCCCGTTGCCCGATTGCCGGTCTAGAGTGCGCAAAAACCC‑GTCCTGTTCTGTAGGGGAGTTTCAGACACGCCGTTCCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GGTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTcc                                                                                                                                                        <  1:813646/251‑1 (MQ=255)
                                                                                           gATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACACTAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGcc                                                                                                                                                    >  1:175171/1‑249 (MQ=255)
                                                                                              cTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGCGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAAAAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCAATTATTTATTCCCGCCAgatg                                                                                                                                               >  2:240612/1‑251 (MQ=255)
                                                                                                             ccgatccaggggcgggAAGGGGAATGACCGTCGCTGAT‑CGGCCGGCCTGCGGGTTGGGTTTGCTGTACCCGTTGCCCGGTGGCCGGTCCAGAGTGGGCCAAAACAC‑GTCCGGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAGGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTGGTCAGCCCTTTTTCACGCTAAAAGCGATTAGTTATTCCCGCCAGATGATATGGCAAAGTTtg                                                                                                                                <  2:195687/237‑1 (MQ=255)
                                                                                                                   cAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCt                                                                                                                          <  1:410524/251‑1 (MQ=255)
                                                                                                                                  gAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAACGTTTGTGATCTTTTTCGCGGCATaa                                                                                                            >  1:223099/1‑250 (MQ=255)
                                                                                                                                   aaTTACCGTCGCCGAT‑CAACCCGCTTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACa                                                                                                          <  1:347774/251‑1 (MQ=255)
                                                                                                                                     ttACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAAc                                                                                                           >  1:23061/1‑248 (MQ=255)
                                                                                                                                     ttACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGAAA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAAc                                                                                                           >  1:815270/1‑248 (MQ=255)
                                                                                                                                               ccaaacAACCCGCCTCCCGGTTTTGTTTTCCGTACCCGTGGCCCGATGGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCGGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATAGGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCa                                                                                               <  2:98574/246‑1 (MQ=255)
                                                                                                                                                                cGGTTTTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTc                                                                              <  1:560120/251‑1 (MQ=255)
                                                                                                                                                                              gTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGATGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTAACTGt                                                                >  2:615935/1‑251 (MQ=255)
                                                                                                                                                                                ccccGTTTCCGGATTGCCGGTCCAGAGGGCGCAAAAACAC‑TTTCTGTTTTGGAGGGGAGTTTAAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTc                                                               <  2:477451/249‑1 (MQ=255)
                                                                                                                                                                                    gtttcccGATTGACGGTCCAGAGTGCGCCAAAACAC‑GTCCTTTTCGGGAGGGGAGGTCCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCa                                                          <  1:147/247‑1 (MQ=255)
                                                                                                                                                                                       gCCCGATTGCCGGTCCAAATTGCGCCAAAACAC‑GTCCTGTTCTGGAGGTGAGTTTCAGCCACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTTACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGTCAAAGTTTGTGATCTTTTTCGCGGCATATCAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCTCCAGa                                                        <  1:858383/250‑1 (MQ=255)
                                                                                                                                                                                        cccgttggcgggTCCAGAGGGCGCCAAA‑CACCGTCATGTTCTGGAGGGGAGTTTCAGAAACGCCGTTTCTTGGCTGAAAGTCGATCCGCTTTAAAAACAATAATGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGAcc                                                      <  2:160119/241‑1 (MQ=255)
                                                                                                                                                                                           gATTGCCGGACCAGAGGACGCCAAAACAC‑GACCTGTTCGGGGGGGGAGTTTCAGACACGCCGTTTCTTGCCGGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTTTGATCTTTTTCGCGTCATAACAGAATGCGGGCAAAAACATCTTTGATTTCTCCATCTTCACTGTCCGCCAGACCAAt                                                   <  2:359231/251‑1 (MQ=255)
                                                                                                                                                                                                     ccAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCg                                         <  1:699524/251‑1 (MQ=255)
                                                                                                                                                                                                              gcCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCG‑CAAAAAAGt                               >  1:202724/1‑251 (MQ=255)
                                                                                                                                                                                                                cAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTc                              <  1:250668/251‑1 (MQ=255)
                                                                                                                                                                                                                    acac‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATACGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAga                                                                                                                                                 >  1:111106/1‑132 (MQ=255)
                                                                                                                                                                                                                     cac‑GTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGt                         >  1:228938/1‑251 (MQ=255)
                                                                                                                                                                                                                           ccTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCaa                     >  1:397878/1‑250 (MQ=255)
                                                                                                                                                                                                                              gTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCg                 <  1:227643/251‑1 (MQ=255)
                                                                                                                                                                                                                                tCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCAGACA‑GTTGTCAGCCCTTTTTCACGCTACAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTACCCCTCTTCACTGTCCGCCAGACCAATACCCACTTCGGCAAAAAAGTCCGGGTTCaaaacgt                >  2:443662/1‑246 (MQ=255)
                                                                                                                                                                                                                                             ttCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGATTTAAACACCATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTAGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTcc  >  2:190841/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
GGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAT‑CAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAC‑GTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACA‑GTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCC  >  NC_000913/1306492‑1306977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑