| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,578,548 | G→A | Q264* (CAG→TAG) Q264* (CAG→TAG) |
mcrB ← mcrB ← |
5‑methylcytosine‑specific restriction enzyme subunit McrB 5‑methylcytosine‑specific restriction enzyme subunit McrB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,578,548 | 0 | G | A | 98.1% | 178.3 / ‑3.6 | 53 | Q264* (CAG→TAG) Q264* (CAG→TAG) | mcrB mcrB | 5‑methylcytosine‑specific restriction enzyme subunit McrB 5‑methylcytosine‑specific restriction enzyme subunit McrB |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (28/24); total (29/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01 | |||||||||||
CAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCG > NC_000913/4578311‑4578781 | caacaaCGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCGTGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTaa < 1:508375/251‑1 (MQ=255)caacaaCGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTaa < 1:493834/251‑1 (MQ=255) aacGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGACTCGTTCTTCATCGTTTTAGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACCAAAATTGTAAAat > 1:32925/1‑250 (MQ=255) acGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAatat > 1:297857/1‑251 (MQ=255) agagatCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTc < 2:120501/251‑1 (MQ=255) gATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAACTATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGt > 1:762281/1‑251 (MQ=255) tgatATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACg > 1:342083/1‑250 (MQ=255) atatAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGAt > 1:807974/1‑250 (MQ=255) tataAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGAt > 1:68966/1‑249 (MQ=255) aTTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGt > 1:111318/1‑250 (MQ=255) ctcCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGCAGCCGACTCCATTCGGACGTTACCCCTGtata > 2:114114/1‑250 (MQ=255) ctcCGGGACATAGAATCGTTCTTCAACGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGATCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTAGGCCGATAGCCCTGtata > 1:480622/1‑250 (MQ=255) gggACATAGAATCGTTCTTCATCGTTTTCGGCGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAATCCGACGCCATTCGGACGATAGCCCTGTATAAAATc > 1:178961/1‑251 (MQ=255) gggACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATc < 1:590922/251‑1 (MQ=255) cATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTATAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCa < 2:155750/251‑1 (MQ=255) aTAGAATCGATCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGCTT‑TCATCTATACTAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGAACGATAGCCCTGTATAAAATCCTCa > 2:429412/1‑250 (MQ=255) tcATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTATCATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCAGCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAATCCGTAGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGa < 2:631830/248‑1 (MQ=255) tCGTTTTCGGAGGTGGTTAGGGTAACAGACCAGTTTTCACCTCGTGTATCATGTTCCATAAACATCATCACTTCGCCAAATACTTTACTGAGATTGTCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGACATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGAt < 2:762281/250‑1 (MQ=255) tttCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCCTTCGGCCGATAGCCCTGTATAAAATCCTCATCGCTATAAGATTGATTGAAc > 1:811452/1‑251 (MQ=255) tttCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGaa < 2:111318/250‑1 (MQ=255) gTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTAGAAAATCCTCAGATCTATAATATTGATGGAACTGAACCa < 2:801233/251‑1 (MQ=255) tAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCat > 1:402749/1‑251 (MQ=255) cagtccAGTTTTCACCTCGTTTATCATGTTCCAATAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACTCGTTGc < 2:728256/247‑1 (MQ=255) cAGACCAGTTTTCAACTCGTTTATCATGTTCCATTTACTACAGCCCTTCGCCAAATTCTTTACTGTGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGATGCCATTCGGACGATAGCCCTTTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATGGACGCGTTGc < 2:83587/251‑1 (MQ=255) agtccAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCg < 1:278486/248‑1 (MQ=255) aGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCg < 1:319789/251‑1 (MQ=255) aaCATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAATCCGACGCCATTCGGACGATAGCCCTGTATAAAATCATCATAGCTATAAGATTGATGGAAATGACACATATTTACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACg > 2:464517/1‑251 (MQ=255) aCATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACgg < 1:12759/251‑1 (MQ=255) atcaCTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGc > 2:206029/1‑251 (MQ=255) cccTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGGCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGcaa < 2:63024/249‑1 (MQ=255) cGCCAAATACTTTACTGAGATTGGCACGATTGATG‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAg < 2:297857/251‑1 (MQ=255) cAAATACTTTACTGAGATTGGCACGATTAATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTATTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGtt > 2:442693/1‑251 (MQ=255) gtGGCACGATTTATTGTCA‑CTATAATAAAAAAATACGTTTTCGCTGTCTGCTCCTTAGCTTGCTAACAAAAATTGTAAAATATGCCGGCTTTACGTCGGAAGCCGAAGCCATTCGGACGATAGCCCTGTATAAAAGCCTCATAGCTATAAGATTGTTGGAACTGAACCATATGGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGggcgg < 2:306182/250‑1 (MQ=255) tGGCCCGATTGATT‑TCATCTATAATAAAAATATTCTTTGTCTCTGGCTGCTCTTTAGCGTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGTCGTGCAACAAAGGTTTTTCCAACGCCGggcgg < 2:414129/250‑1 (MQ=255) gCACGATTGGTT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAACTTGCTAACAAAAATTGTAAATTATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAAACCTCATAGCTATAAGATTGATGGACCTGAACCATATTGACGCGTTGCGGCGCCTTTTCTCCTGTCAGCACGTAAGCCAGACGGCGTGCAACAAAGGCTTTACCAACGCCGGTCGGccc > 2:379230/1‑251 (MQ=255) tgatt‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTggag < 1:723763/250‑1 (MQ=255) gatt‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTggagg < 1:193828/250‑1 (MQ=255) tt‑tCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGAGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGat > 2:755087/1‑250 (MQ=255) t‑tCATCTATAATAAAAATATACTTTTTATCTGACTGCTCTTTAGCTTGCTAAAAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGata > 1:632725/1‑250 (MQ=255) tCATCTATAATACAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGAAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATACGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCACCAAAGGTTTTTCAAACGCCGGGCGGCCCCTGGGGGataat > 2:602859/1‑251 (MQ=255) tCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATAtt > 1:548140/1‑251 (MQ=255) cTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATAttt > 1:554318/1‑251 (MQ=255) aaaTATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACTTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGAGCAACAAAGGTTTTTCCACAGCCGGGCGGCCCCTGGAGGATAATATTTATTTTgttg > 2:24638/1‑248 (MQ=255) aCTTTTTCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTaa < 1:434999/250‑1 (MQ=255) tttttCTACGGCTGCTCTTTATCTTGCTAACAAAAATAGGAAAATATTCCGTCTTTACTTCGGAAGCCGACGGCATGCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCGTTTCTCCTGTCAGCAAGTAAGCCAGACGGCTTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTGTGATTGTTAATc < 2:639744/250‑1 (MQ=255) ttttCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGt > 1:873964/1‑251 (MQ=255) ttCTCTGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGttt > 1:807783/1‑251 (MQ=255) tctGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGGGCAAAAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTg > 2:517782/1‑249 (MQ=255) ctGGCTGCTCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGt > 2:350590/1‑251 (MQ=255) ctgctCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGCGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCg > 2:458907/1‑251 (MQ=255) gctCTTTAGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCg < 1:458907/249‑1 (MQ=255) aGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTACAACGCCGggcggc > 2:327692/1‑196 (MQ=255) aGCTTGCTAACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTACAACGCCGggcggc < 1:327692/196‑1 (MQ=255) | CAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATT‑TCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCG > NC_000913/4578311‑4578781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |