Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,297	G→A	R80C (CGT→TGT)	proQ ←	RNA chaperone ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,297	0	G	A	96.1%	228.4 / NA	78	R80C (CGT→TGT)	proQ	RNA chaperone ProQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/46);  minor base C (2/1);  total (31/47)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTC  >  NC_000913/1915050‑1915542                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        aCCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGt                                                                                                                                                                                                                                                      <  1:526666/251‑1 (MQ=255)   cGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTg                                                                                                                                                                                                                                                    <  1:312033/251‑1 (MQ=255)     gCTTTTGCGCACGTGGTTTACGTTCAGCGCCTTCTGTGCGGCGTTGTGTAGGCTGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTg                                                                                                                                                                                                                                                    <  2:472493/249‑1 (MQ=255)                    gTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGCCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGGTTTTTCGCTTGCTGTTCAGCAAGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGATTGTGAGCATGCTCTACATGTTGCTCGACCAGCTCACCGCATGGGGTGTCGTCAAGATCAACAATCGTTGCGCTGGGTTTacaaccg                                                                                                                                                                                                                                   >  2:587927/1‑245 (MQ=255)                        aCGTTCAGCGCCTTCTGTGCGGCGTGGCGTAGTCGTACGTTGCTTGCGTTCGCTGCGCGGTGCGTCTTCTTTCTCCCCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCGGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTaa                                                                                                                                                                                                                                <  2:311520/250‑1 (MQ=255)                          ggtCAGCGCCTTCTCTGCGGCGTGGCTTAGTTGGACGTGGGTTGCGGTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCAGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGa                                                                                                                                                                                                                             <  2:49576/249‑1 (MQ=255)                                 gcCTTCTGTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGc‑c                                                                                                                                                                                                                     <  1:430599/251‑1 (MQ=255)                                      cttTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTc                                                                                                                                                                                                                >  1:185062/1‑251 (MQ=255)                                           cgtcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGCACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGCCCAGCTCACCGCATGGGTTGCCGTTAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAgt                                                                                                                                                                                                           <  1:142529/248‑1 (MQ=255)                                           cggcgTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAgt                                                                                                                                                                                                           <  1:68760/251‑1 (MQ=255)                                            ggcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATTTTGCTCGTCCAGCTCACCGCCTGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCACA‑CTCGAAgtg                                                                                                                                                                                                          >  2:617397/1‑251 (MQ=255)                                               gtggcgtAGTCGGACGTGGCTTGAGTTCGCGGCGCTGTGCGTCTTCTTTCTCACCAGCAGTTGCTGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGTTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTTCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACCCGTTGCGTCGGGTTTAACACCTTAAAGATAACGC‑CAGCTCGAAGTGTag                                                                                                                                                                                                       <  1:64997/251‑1 (MQ=255)                                                 ggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTag‑ag                                                                                                                                                                                                    <  1:6643/251‑1 (MQ=255)                                                  gcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTag                                                                                                                                                                                                       >  1:139032/1‑248 (MQ=255)                                                    gtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACg                                                                                                                                                                                                 <  2:140166/251‑1 (MQ=255)                                                          ggACGTGGCTTGAGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGAAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGAGCTTTCGCTTCTTCCACCTACTTGCGAGCATGCTCTACATGTTGCTCGTCCCACTCACCGCATGGTTTGCCGTCACGATCCGCCCCCGTTGCGCCTGGTTTAACAACGCAAAGATTACGC‑CAGCTCGAAgtgtgggaacgttacgc                                                                                                                                                                                            >  2:90318/1‑238 (MQ=255)                                                                ggcgtGCGGTCGCGGCGCGGTGCGGCGTCTCTCTCACCAGCAGTGGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGGCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACATCGTAAAGATAACGC‑CAGCTCGAAGTGTAT‑AGACGTAAAGCGGATCg                                                                                                                                                                                     <  2:369891/247‑1 (MQ=255)                                                                    tGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCaa                                                                                                                                                                                  <  1:350960/250‑1 (MQ=255)                                                                    tGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCa                                                                                                                                                                                   <  1:760310/249‑1 (MQ=255)                                                                    tGCGTTCGCGGCGCGGGGCGTCTTCTTTCTCACCAGCAGTTGCGGAAGCTTCGCGTTTTTTCGCTTACTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCACGCTGCTTGCGAGCATGCTCTACATGGTGCTCGTCCAGCTCACCGCCTGGGTTGCCGTCACGATCGACCCCCGTTGCGCCGGGTTTAACCCCGTAAAGATAACGC‑CCGCCCGAAGTGTCGCAG‑CGTAAAGCGGCTCGCAAt                                                                                                                                                                                 >  2:542024/1‑251 (MQ=255)                                                                        ttCGCGGCGAGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCCGCTTCGCGTTTTTTCGCTTGCTGCTCAGCACGCTGTGCCTGCACACGTGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGACGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGCAAAGTTAACGC‑CAGCTCGAAGGTTAG‑AGACGTAAAGCGGATAGCAATTGCg                                                                                                                                                                             >  2:174798/1‑251 (MQ=255)                                                                         gcgcggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGt                                                                                                                                                                            <  1:699392/250‑1 (MQ=255)                                                                           gcggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGGTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGttt                                                                                                                                                                          <  2:437405/251‑1 (MQ=255)                                                                                  ggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCCTGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTc                                                                                                                                                                     >  2:267723/1‑249 (MQ=255)                                                                                  ggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTc                                                                                                                                                                     >  1:271593/1‑249 (MQ=255)                                                                                  ggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGACATTGCGTTTTGCTc                                                                                                                                                                     >  2:611263/1‑249 (MQ=255)                                                                                                  cACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAg                                                                                                                                                     <  1:776569/249‑1 (MQ=255)                                                                                                    ccAGCAGTTGCGGCAGCTTCGCGTTGTTTCTCTGTCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTGGCTCGTCCAGCTCGCCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGTCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGGAG‑AGACGTAAAGCGGATCTCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAac                                                                                                                                                 <  2:778153/251‑1 (MQ=255)                                                                                                     cagcagTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTAGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAac                                                                                                                                                 >  2:211026/1‑250 (MQ=255)                                                                                                     cagcagTTGCGGCAGCTTCGCGTTTGTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAaca                                                                                                                                                <  1:718720/251‑1 (MQ=255)                                                                                                        cagTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACg                                                                                                                                              >  1:322341/1‑250 (MQ=255)                                                                                                         agTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGAt                                                                                                                                            >  1:464350/1‑251 (MQ=255)                                                                                                          gTTGCGGCAGCCTCGCGTTTTTTCGCCTGCTGTTCAGCACGCTTGGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGGCAAGATCGACCCACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATc                                                                                                                                           <  2:350823/251‑1 (MQ=255)                                                                                                            tGCGGCGGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCg                                                                                                                                          <  2:709803/250‑1 (MQ=255)                                                                                                            tGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCCGAACACGCGCTTTCGCTGCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGTCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCg                                                                                                                                          <  1:860645/250‑1 (MQ=255)                                                                                                             gCGGCAGCTTCTCGTTTGTTCGTTTGCTGTTCAGCCCGCTGGGCCGGACCCCGAGCTTTCGCTTCTTCAAGCGGCGTGGGAGCATGCTCTACATGTTGCTCGTTCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAATATAACGC‑CAGCTCGAAGTGTAG‑AAACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCAGTTCCCCAGCAACACGAGCGa                                                                                                                                         <  2:740655/250‑1 (MQ=255)                                                                                                             gCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGAc                                                                                                                                        <  1:226518/251‑1 (MQ=255)                                                                                                                gCAGCTTCTCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCaa                                                                                                                                     <  1:673709/251‑1 (MQ=255)                                                                                                                gCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCaa                                                                                                                                     <  1:420510/251‑1 (MQ=255)                                                                                                                  aGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAAt                                                                                                                                   >  1:708698/1‑251 (MQ=255)                                                                                                                   gCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATc                                                                                                                                  <  1:542024/251‑1 (MQ=255)                                                                                                                    cTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATcc                                                                                                                                 >  1:474426/1‑251 (MQ=255)                                                                                                                            tttttCGCTTTCTGTTTCGCGCGCTGTGCCGGATCAAGCTCTTTCGCTTCTTCATGCTGCTTTCGAGCATGCTCTACATGTTGCTAGTCCAGCTCACCGTATTGGTGGACGTCAAGATCGACACACGTTTCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGGAAAGCGGATCGCGATTGCGTTTTGCACAGGTTCACGTCCCCAGCAACAAGATCGACCAAATCCTGaaaaa                                                                                                                          <  2:455360/250‑1 (MQ=255)                                                                                                                                         gTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAg                                                                                                             <  1:140394/250‑1 (MQ=255)                                                                                                                                             aGCACGCTGTGCATGAACACGCGCTTTAGCTTATTCAAGCTGCATGCGAGCATGCTCTACATGTTGCTCGTCCAGCACACAGCATGGGTTGACGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTACAGATAACGC‑CAGCTCGAAGTGTAG‑AGCCGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGATCATTTCACCAGCAACACGATCGACCAACTCCTGAAAAATACAGCTTTTCAGCGGAc                                                                                                        >  2:842575/1‑251 (MQ=255)                                                                                                                                                  gCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAacac                                                                                                                                               <  1:567401/207‑1 (MQ=255)                                                                                                                                                  gCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAacac                                                                                                                                               >  2:567401/1‑207 (MQ=255)                                                                                                                                                  gCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCt                                                                                                   >  1:35697/1‑251 (MQ=255)                                                                                                                                                           gAACCCGCGCTTTCGCTTCCTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACCCCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCtt                                                                                            <  2:537082/249‑1 (MQ=255)                                                                                                                                                           gAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTAAGCggcccagattaaccttcc                                                                                          >  2:584517/1‑235 (MQ=255)                                                                                                                                                             acacGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAAAACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGc                                                                                        >  1:106040/1‑251 (MQ=255)                                                                                                                                                                cgcgCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACt                                                                                     <  1:569341/251‑1 (MQ=255)                                                                                                                                                                   gcTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGaa                                                                                  <  2:166273/251‑1 (MQ=255)                                                                                                                                                                        cGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCGCGAAGTGTAG‑AGACGTAAAGCGGATCGCAAGTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGAGCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAg                                                                              <  1:90318/250‑1 (MQ=255)                                                                                                                                                                                      tctttcgcgCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGTCCTCAAGATCCACACACGTTGCTCCGGGTTTAACACCGTAAAGATATCGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCTCTTCACCTTCCGCACTGAAACATTGGGGAAAACGtt                                                                 <  2:607229/241‑1 (MQ=255)                                                                                                                                                                                      gctTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCg                                                               <  1:475312/251‑1 (MQ=255)                                                                                                                                                                                                    cctACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGAt                                                 <  2:211022/250‑1 (MQ=255)                                                                                                                                                                                                     ctACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGACCGACACACGTCGCGCCGGGTTTAACACCGTAAAGATAACGA‑AAGATCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTACCCAGAAAAACGATCGACAAAATACTGAAAAATAACGCTTTTCACCGGACGCGATTCCCCTACCGCACTGAAACAGAGGGGCAAACGTTCGGCCAGAAACGCGagt                                                >  2:690105/1‑249 (MQ=255)                                                                                                                                                                                                     ctACATGTTGCCCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATTGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCGCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCCCCGTCCTCACTGAAACAGTGGGTAAAACGGTCGGCCAGAAACGCGa                                                  <  2:708698/249‑1 (MQ=255)                                                                                                                                                                                                       aCATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCg                                                                                                                     >  1:527802/1‑180 (MQ=255)                                                                                                                                                                                                       aCATGTTGCTCGGCCAGCTCACCGCATGGGTTTCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATATCGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAAATGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCg                                                                                                                     <  2:527802/180‑1 (MQ=255)                                                                                                                                                                                                         aTGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTActt                                            <  1:772342/251‑1 (MQ=255)                                                                                                                                                                                                             tGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTActtctt                                         <  1:743380/250‑1 (MQ=255)                                                                                                                                                                                                             ggCTCGTCCAGCTCTCCGCATGGGTGGCCGTCAAGATCGGCACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCTTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTActtctt                                         <  2:748153/249‑1 (MQ=255)                                                                                                                                                                                                               cTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAT‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAACAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACCGTGGAGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTAc                                      >  2:784224/1‑251 (MQ=255)                                                                                                                                                                                                                       gCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACATTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTGCTTCTTTACTGCTATTc                              <  1:433454/251‑1 (MQ=255)                                                                                                                                                                                                                         tCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGAGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCa                             >  2:305874/1‑250 (MQ=255)                                                                                                                                                                                                                            ccGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACtt                         <  1:773259/251‑1 (MQ=255)                                                                                                                                                                                                                                atgtgTTGCCGTCAAGATCGACACAAGATGCGCCGGGTTTAACAGCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGAACGCAATTGAGTTTTGCTGAGGTTCATTTCCCCAGCAACACAATCGACCAAATCCAGAAAAATACCGATTTTAAGCGGACGAGCTTCACATTCCGAACTGAAAAAGTGGGGAAAACGCTCGGCACGAAACGCGATTGCTTCTTTaacgatattccaatacggt                     >  2:843329/5‑233 (MQ=255)                                                                                                                                                                                                                                aTGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGt                     >  1:12246/1‑251 (MQ=255)                                                                                                                                                                                                                                       gCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTAAGGTTCATTTCCCCAGCAACACGATCGACCACATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAtttt              >  1:526284/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGTTTTTcc            >  2:497575/1‑250 (MQ=255)                                                                                                                                                                                                                                          gTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTcc            >  1:249729/1‑250 (MQ=255)                                                                                                                                                                                                                                            cAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCa                                                                                                <  1:515781/164‑1 (MQ=255)                                                                                                                                                                                                                                             aaGAGCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCAAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGa        <  1:24008/251‑1 (MQ=255)                                                                                                                                                                                                                                               gATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGCAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCa                                                                                                                                                      >  1:295213/1‑107 (MQ=255)                                                                                                                                                                                                                                               gATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTGCCCCa                                                                                                                                                      <  2:295213/107‑1 (MQ=255)                                                                                                                                                                                                                                                   gACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTc  >  1:92613/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        ACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGC‑CAGCTCGAAGTGTAG‑AGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTC  >  NC_000913/1915050‑1915542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑