| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,823,162 | C→T | H255Y (CAC→TAC) | spoT → | bifunctional (p)ppGpp synthase/hydrolase SpoT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,823,162 | 0 | C | T | 98.9% | 285.2 / ‑4.2 | 92 | H255Y (CAC→TAC) | spoT | bifunctional (p)ppGpp synthase/hydrolase SpoT |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (52/38); total (54/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01 | |||||||||||
TTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCC > NC_000913/3822916‑3823407 | ttAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTc > 1:289826/1‑251 (MQ=255) cacaTTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACAGTTCTCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATAATCCAGAAGATTATTTCTGAAATCGAAGGGCGTTTGAAGGAAGCGGGAATACCGTGCAGCGTCAGTGGTCGCGAGAAGCGTCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGAAGCGTTTTTACTCGATCATGGACAt > 2:440110/1‑251 (MQ=255) tAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑Ac < 1:800229/250‑1 (MQ=255) tAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑Ac < 1:793704/250‑1 (MQ=255) aaaaaCCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CGCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACg < 2:31985/250‑1 (MQ=255) aaaCCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGACAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGCCAGTGGTCGCGCGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGAGTTTTTACTCGATCATGGCCATCT‑ACGCt > 2:731470/1‑251 (MQ=255) ccGAACTCGAAGAGCGGGGTTTTGAGGCGCTGTAGCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTc < 2:721951/251‑1 (MQ=255) gAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGAAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCcg > 1:602518/1‑251 (MQ=255) cgcTCGACGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTAACGCGTAATCAAAGAAGTGGCGCAAGCCGCGCGCGGAAACC‑GTAACGAGATGATCCAGAAGTTTCTGTCTGAAAACGAAGGGCGTTTGCAGGAAGCGGGAATACCGGGCCGCGTCAGTGGACGCGTGAAGAATATTTATTAGCTTTACTGAAAAGTGGTG‑CTAAAAGAGCAGCGTTTTTACTCGAAAATGGAAATCT‑aatctttcagg > 2:797693/3‑241 (MQ=255) aCTCGAAGAGCTGGGATTTGAGGCGCTGTATCCCAACCGTTATCGCGGAATCAACGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTAACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTAACTCGATCATGGACATCT‑ACGCTTTCcg > 2:305696/1‑249 (MQ=255) cTCGAAGAGCTGGGTTTTGAGGAGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGAGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGCAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGGCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCGGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCcgcgg > 2:119510/1‑250 (MQ=255) gAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGAt > 1:855201/1‑251 (MQ=255) aaGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATc < 1:30402/251‑1 (MQ=255) agagCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑AAGCTTTCCGCGTGATCg > 2:420496/1‑251 (MQ=255) gagCTGGGGTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCAGCGCGCGGCAACC‑GTCAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATCCCGTGCCGCGTCAGTGGGCGCGAGAAGCATCTTTATTCGATTTCCTGCAAAATGGTG‑CTCAAAGAGCGGCGTTTTTGATCGCTAATGGACATCT‑ACGCTTTACGAGTGATCGt > 2:572561/1‑251 (MQ=255) agCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGGTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTc < 2:93463/251‑1 (MQ=255) gCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTc > 1:862284/1‑250 (MQ=255) gggTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCaa < 2:709019/249‑1 (MQ=255) gTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGAt > 1:284332/1‑251 (MQ=255) gagccccTGTATCCCAACCGTTAACGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTg < 2:230143/244‑1 (MQ=255) gcTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCt < 2:176720/251‑1 (MQ=255) tGTACCCCAACCGTTATCTCGTAATCAAATAAGTGGTGAAAGCCGCGCGAGGCAACC‑GGAAAGAGATGAGCCAGAAGATTCTTTCGTAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGAGTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATGCTGACTCCTg < 2:790532/250‑1 (MQ=255) tCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGACAGCCGCGCGCGGCAACC‑GTACAGAGATGATACAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACAGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGCAACCTGTTATc > 2:75338/1‑251 (MQ=255) cAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATcgcg < 2:204287/251‑1 (MQ=255) aaCCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGt > 1:303073/1‑251 (MQ=255) ttATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTgg > 2:467428/1‑251 (MQ=255) cgcgTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGACAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGcc < 1:408769/250‑1 (MQ=255) gTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGcc > 1:147388/1‑247 (MQ=255) gTAATCAAAGAAGGGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTAATCGATCATGGACATCT‑ACGCTTTCCGAGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGATGGGca > 2:860561/1‑246 (MQ=255) aaaGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGACAGATGcac > 2:154971/1‑249 (MQ=255) gaagTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCACAGCGCAGCGTTTTTACTCGATCATGTACATCT‑ACGCTTTCCGCGTGATCTTCATTGATTCTGACACCTGTTATCGCGTGCTGGGCCTGATCCACAGCCt > 2:214659/1‑251 (MQ=255) aagTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACCCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCta < 2:185539/251‑2 (MQ=255) agTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGt > 1:187921/1‑251 (MQ=255) taaaGCCGCGCGCGGCATCC‑GTAAAGAGATGATCCAGAAGAGTATTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGCG‑CTCAAAAAAAAGCGGTTTTACTCGATCATGGACCTCT‑ACGCTTTACGCGAGTGCGTCAATGATTCTGCCACCTGTTATCGCGAGCTGGGCCAGATGCAAAGACTGAACAAGc > 2:472779/2‑251 (MQ=255) gAAAGCCGCTCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTATGAACTCGAAGGGCGTTTGCAGGAAGCGAGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGAG‑CACAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGAGATCGTCAATGCTTCTGACACCTGTTATCGCGTGCTGGGCAAGATGCACAGCCTGTACacgc > 2:513260/1‑248 (MQ=255) aGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCcgc > 1:5472/1‑251 (MQ=255) gCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTCCTGAAATCGAAGGGCGTGTGCAGGAGGCGGGAATACAGGGCCGGGTCAGTGGGCGCGAGAAGCATCTTTATTCGATTTACCGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATGT‑ACGCTTTCCGCGTGATCGTAAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCcgcg < 1:811688/251‑1 (MQ=255) cgcgcgGCAACCAGTAA‑GAGATTATACTGAATATTCTTTCTGAAATCAAAGTGCGGGTGCAGTAAGCGGGAATGCCGGGCCGCGTCAGTGGTCGCGAGAGGTATCTTTATTCGATTTACGGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCAGGGACATCT‑ACGTTTTCCGCGTGATCGTCATTGATTCTGACACCTTTTTTCGCGTGCGGGGACAGATGCACAGCCTGTACAAGCCGCGTCCg < 2:484290/251‑1 (MQ=255) cgcgcgGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCg > 1:493794/1‑251 (MQ=255) cgcgcgGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCg < 1:420496/251‑1 (MQ=255) cgcgGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCggg > 1:232241/1‑251 (MQ=255) ggggCAAAC‑GTAAAGAGTGGATCAAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGGGCCGCGTCAGGGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGAGCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGc < 2:20583/249‑1 (MQ=255) aCC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTg > 1:610328/1‑251 (MQ=255) gTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGTGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTCCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaaa < 2:341324/251‑1 (MQ=255) gTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaaa < 1:731470/251‑1 (MQ=255) gatCCAGAAGATTCTTTCTGAAACCGAAGGGCGTTTGCAGGAAGCGGGAATACCGAGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGACAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCg < 1:8195/251‑1 (MQ=255) aagaTTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTcc > 1:325877/1‑251 (MQ=255) agaTTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTcc > 1:685849/1‑250 (MQ=255) agaTTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTcc > 1:314654/1‑250 (MQ=255) tctttctGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCaaaa < 1:774335/250‑1 (MQ=255) tctttctGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCaaa > 2:211787/1‑249 (MQ=255) ttctGAAGTTGAAGGGAGTTTGCAGGTAGCGGTAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATTTTTATTCGATTTACTGCAAAAGGGTG‑TTCAAAGGGCAGAGTTTTTACTCGATTATGGACATCT‑ACGTTTTCCGCGTGATCGTCAATGATTCTGACTCCTGTTATCGCGTGCTGGGCCAGATGCACAGCTTGTACAAGCCTCGTCCGGGCCGCGTGAAAGACTATTGCGCCATTCCAAAAGCg < 1:63683/250‑1 (MQ=255) aaaTCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGATTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGc > 1:740762/1‑251 (MQ=255) gaagggatgtggcatgAAGCAGGACTACCGTGCCGCGTCAGTGGGCTCGGTAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTTAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGGGTGAGCGGCAATGATTCTGACACCTGTTATCGCGGGCTGGGCCAAATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCa < 2:339093/236‑1 (MQ=255) aGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGt > 2:106349/1‑251 (MQ=255) gggCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCTATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGAGTCTGACACCTGTTATCGCGTGCTGGGCCAGAGGCACAGCCTGTACAAGCCGCGTCCGTGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTc < 1:35820/251‑1 (MQ=255) ttcaggaacggggaagcccGTGCCGCGTCAGTGGTCGCGAGAAGAATCTTTATGCGATTAATGGCAAAATGGGG‑CTAAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCGTTCCGCGTGATCGTCAATGTTTTTGACACCTTTTAGCGCGTGCGGGGCCAGTTGCTCAGCCTGTACAAGCCGCGGCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGca < 2:324997/233‑1 (MQ=255) gCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGCAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCCAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACCCCTGTTATCGCGTGCTGGGCCCGATGCACAGCCTTTACAAGCCGCTTCCGTGCCGCGTGCAACCCTATATCCCCATTCACAAAGCGAACGGCtctacgcccttccacacctagcg > 2:214523/1‑229 (MQ=255) gggAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatg > 1:825924/1‑250 (MQ=255) ggAATCCCGGGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACACCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGGGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatgat < 2:187921/251‑1 (MQ=255) ggAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatgat > 1:286825/1‑251 (MQ=255) gAATACCGTGCCTCGTAAGTGGTCGCGAGAAGCAGCTTTATTCGAGTTACTGCAAAAGGGTG‑CGCAAAGAGCAGCGTTTTTTCTCGATCATGGACATCT‑ACGCTTGCCGCGTGTTCGACAATGATTCTGACACCTGTAATCGCGTGCTGGGCCAGATGCACAGCCGTTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATc < 2:289826/251‑1 (MQ=255) gAATACCGTGCCGCGTCAGTGGTCGCGAGACGCATCTTTATTCGATTTAATGCAAAATGGTG‑CTCAAAGTGCAGCGTTTTTACTCGAACATGGACATCT‑ACGCTTTCCGCGTGAGCGTAAATGATTCTGACACCTGTTATCGCGTGCTGGGCCCGCTGCACAGCCTGTACAAGCCGCCACCGGGCCGCGTGCAAGACTATATCGACATTCCACAAGCGAACGGCTATCAGTCTTTGCGCACCTCgatgttc > 2:801120/1‑248 (MQ=255) ctACCGTGCCGCGTCAGTGGACGCGAGAATCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCCGATGCACAGCCTGTACAGGCCGCGTTCGGGCCGCGTGAAAGACTATATCGCCATTCCAAACACGCACGGCTATCAGTCTTTTCCCACCCCgatgatagg > 2:27401/2‑248 (MQ=255) aCCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGcc > 1:506272/1‑251 (MQ=255) cGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCg < 1:36760/251‑1 (MQ=255) gTCAGGGGTTGCGAGAAGAATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGGGCAGTGTTTTTACACGATCATGGACATCT‑ACGCTTTCCGCGGGATCGTCAATGATTCAGACACCTGTTATCGCGGGCGGGGCCAGATGCACAGTCTGTCCAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGTGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGgtgt < 2:589287/251‑1 (MQ=255) cAGTGGTAGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCCCGGTGTGc > 2:173867/1‑251 (MQ=255) ggggcgcgGGAAGCATCTTTATTCGAGTTACTGCAAAATG‑TGTCTCAAAGTGCGGTTTTTTTACTCGATCATGTACATTT‑ACGCTTTCCGCGTTATCTGCAATTATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTGTGCACACCTCGATGAGCGTCCCGCACGGTGTGCCgg < 2:862284/247‑1 (MQ=255) ggTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACGCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCgg < 1:75338/250‑1 (MQ=255) ggTCGCGAGAAGCATCTTTATTCGAATTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGAGCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATTCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGACAGACTATATCGCCATTCCAAAAGCGAACGGCCAACAGTCTTTGAACACCTTGATGATCGGCCCGCACGGTGTGCCggg > 2:751308/1‑250 (MQ=255) aGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGAACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAATAGCGAACGGCTATCAGTCATTGAACACCTCGATGATCGGCACGCACGGTATGCCGGTTGGGGTcc > 1:481251/1‑249 (MQ=255) cATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTGTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACATCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGAt < 1:70626/251‑1 (MQ=255) tttATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGt > 1:672761/1‑251 (MQ=255) tATTCGATTTACTGCATAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGCGGTCCAGATCCGtcc > 2:536307/1‑249 (MQ=255) cGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGAGCCGGTTGAGGTCCAGACCCGTACCGa > 2:826467/1‑250 (MQ=255) tttACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGAACGGTGTGCCGGTTGAGGCCCAGATCCGTACCGa > 1:845057/1‑247 (MQ=255) aCTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATg > 1:666512/1‑251 (MQ=255) aCTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGAAATCT‑ACGCTTTACGCGTGCTCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGTCAGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTCTCAGTCTTTGCACACATCGATGATCGGCCCGCGCGGTGTGCCGGTTGAGGTCCAGATCCGTACCGCAGatttg > 2:146304/1‑248 (MQ=255) gCAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGGCGGTTGAGGTCCAGAGCCGTACCGAAGATATGGa < 1:211787/250‑1 (MQ=255) cAAAATGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAcc > 1:350845/1‑251 (MQ=255) aaaTGGGG‑CTCAAAGAGCAGCGTTTTTATCCGATCATGGACTCCT‑ACGCTTTCCGCTGGATCTTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAg < 2:685849/251‑1 (MQ=255) tGGTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATg > 1:302451/1‑251 (MQ=255) gTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGt > 2:817893/1‑120 (MQ=255) gTG‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGt < 1:817893/120‑1 (MQ=255) g‑CTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCAGCGTGATCGTCAATGATTCTGCCACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGAGCCGCGTGAAAGACTATCTCGCCATTACAAAAGCGAACGGCTATCAGTCTTTGCACACCTAGTTGATCGGCCCGAACAGTGAGCCGGTTGAGGACAAGATCAGTCCAGAAGATATGGaaccgacagagg > 2:777537/1‑240 (MQ=255) cAAAGAGCAGCGTTTTTACTCGATCATGGAC‑TCTTACGCTTCCCGCGTGATCGTCAATGATTCTGCCCCCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCGTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGgag < 2:511416/250‑1 (MQ=255) aaGAGCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATg > 1:298140/1‑251 (MQ=255) agagCAGCGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATgg > 2:835135/1‑251 (MQ=255) agcagcGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATgggg > 1:784084/1‑250 (MQ=255) gcagcGTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgtg < 1:572561/251‑1 (MQ=255) gTTTTTACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGcc < 1:154971/251‑1 (MQ=255) | TTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACC‑GTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTG‑CTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCT‑ACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCC > NC_000913/3822916‑3823407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |