Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,947	C→A	intergenic (‑242/+669)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,947	0	C	A	100.0%	227.6 / NA	73	intergenic (‑242/+669)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/46);  total (27/46)

TTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAAT  >  NC_000913/3707705‑3708185                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               ttattCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCacaaacaa                                                                                                                                                                                                                                            >  1:37933/1‑247 (MQ=255)  tattCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTAAAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGAt                                                                                                                                                                                                                                        <  1:47342/250‑1 (MQ=255)   attCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGAtt                                                                                                                                                                                                                                       >  1:172033/1‑250 (MQ=255)   attCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTa                                                                                                                                                                                                                                      <  1:697845/251‑1 (MQ=255)      cTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTaaa                                                                                                                                                                                                                                    >  2:429363/1‑250 (MQ=255)        gttCCAATTTTGATGTTTGTTGTTTTTACCCTTTGCAGGGGGTTGTCGCTGCCTGATCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACAGCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTaaaaa                                                                                                                                                                                                                                  <  2:21460/248‑1 (MQ=255)        gCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTaaa                                                                                                                                                                                                                                    <  1:298174/248‑1 (MQ=255)           ccAATTGTGATGTTTGTTGTTTTAACTCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTCTTTTTTGCCCTGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACt                                                                                                                                                                                                                              <  2:243490/251‑1 (MQ=255)             aaTTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGACAATACAGCCAACCGGGATTTACACCACCGGTGAGAATCCACAAACAAAGATTAAAAACACTTc                                                                                                                                                                                                                            >  1:835601/1‑251 (MQ=255)                 gtgATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACTGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCaaa                                                                                                                                                                                                                         <  1:373198/250‑1 (MQ=255)                        ttgttgTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTAtt                                                                                                                                                                                                                 >  1:114374/1‑251 (MQ=255)                         tgttgtTTTAACCCTTTGCAGTGGGTTGTCGCTGACTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGAGCGGATCCTCCATAAAATGACCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTAAACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTAttt                                                                                                                                                                                                                >  1:809212/1‑251 (MQ=255)                             gttTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgca                                                                                                                                                                                                             <  1:224260/250‑1 (MQ=255)                                ttAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgcagca                                                                                                                                                                                                          <  2:114374/250‑1 (MQ=255)                                  taCCCTTTGCAGTGGGTTGTCGCTTCCTGACCTTTTTTGTTTGTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTTCTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCaga                                                                                                                                                                                                        <  1:844688/249‑1 (MQ=255)                                    cccTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGaa                                                                                                                                                                                                     <  1:602895/251‑1 (MQ=255)                                     ccTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAACAAACTTCAAAAAGCTATTTGCAGCAGAGaa                                                                                                                                                                                                     >  2:203200/1‑250 (MQ=255)                                        ttGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGGCGGGTCAACGTTATGAGGTGGGGATGCCGTACTCATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGCATGTGACAACGTAAATACAGCCAACCGGGATTTACACCAACGGTGAGCATCCACAAACAAAGATTAAAAAAAATTAAAACAGCTATTAGCAGCAGAGAAAttt                                                                                                                                                                                                 >  2:81990/1‑251 (MQ=255)                                           cAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtgtg                                                                                                                                                                                              <  1:39366/251‑1 (MQ=255)                                                 gtggTCGCTGCCTTACTTTGGTTGGTTTTTGACCGTGCGGGTCATCGTTATGAGTGGGGGATGTAGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGAACTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGAGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCttcac                                                                                                                                                                                        <  2:248427/248‑5 (MQ=255)                                                 gTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTc                                                                                                                                                                                        >  1:456751/1‑251 (MQ=255)                                                        cTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACAt                                                                                                                                                                                  >  2:459456/1‑250 (MQ=255)                                                        cTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGAGCTACTCCAACATg                                                                                                                                                                                 >  1:28099/1‑251 (MQ=255)                                                                ccTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAAc                                                                                                                                                                         >  2:681966/1‑251 (MQ=255)                                                                      ttgtttttggCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCa                                                                                                                                                                    <  2:850203/250‑1 (MQ=255)                                                                          tttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTGCTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                                                                                                                                                <  2:5043/250‑1 (MQ=255)                                                                          tttttgCCCGGTCGGGACAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATACTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                                                                                                                                                <  1:213329/250‑1 (MQ=255)                                                                           ttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGACCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGATTCCACAACCAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTa                                                                                                                                                               >  2:530411/1‑250 (MQ=255)                                                                                   ggTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGc                                                                                                                                                      <  1:277581/251‑1 (MQ=255)                                                                                   ggTCGGGTACACGTTAAGAGGTGGGGATGCCGTACTAATTCACATCAGTGTAACAGTGCGGTTCCACCATAAAATACCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATAAAGCCAACCGGGATTTACACCAACGGGGAGAATCCACAAACAAAGATTAACAAAACTTCAAACAGCTATTTGCAGCAGAAAAATTTGTGCTACACCACCATGACCAGAACAATCAACTTAATATTTAGc                                                                                                                                                      >  2:610015/1‑251 (MQ=255)                                                                                                      ggTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTAAATATTTAGCAACATTTGGTAATTAAGtt                                                                                                                                   >  2:306292/1‑251 (MQ=255)                                                                                                                    aCTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAAGCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCATCATTTGGTGATTAAGTTTTATGCATTTAAt                                                                                                                      <  1:651201/250‑1 (MQ=255)                                                                                                                       aaatAACATCAGTGTAACAGTGCGGATCCTACATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATTACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGaa                                                                                                                   <  2:837523/247‑1 (MQ=255)                                                                                                                       aaTTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGa                                                                                                                    <  1:668679/249‑1 (MQ=255)                                                                                                                         ttAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGaaaa                                                                                                                 >  1:330358/1‑250 (MQ=255)                                                                                                                          tAACATCCGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTGAGCAACATTTGGTGATTAAGTTTTATGCAGTTAATGaaaaa                                                                                                                <  1:252918/250‑1 (MQ=255)                                                                                                                           aaCATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGaaaaaa                                                                                                               <  1:663850/250‑1 (MQ=255)                                                                                                                                  ttgtAAAAGTGCGGCTCCTCAATAAAATGCCCCTC‑GTGACCTAAAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTATTGCAAAAAATCTGAgg                                                                                                       >  2:516743/2‑251 (MQ=255)                                                                                                                                       aCAGGGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTAAAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAAACGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATGTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAGTTGGTGATTAAGTTTTATGCATTTAAGGAAAAAAATCTGAGGAAAAg                                                                                                  <  2:843985/251‑1 (MQ=255)                                                                                                                                          gTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGG‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTg                                                                                               <  1:609026/251‑1 (MQ=255)                                                                                                                                                 tcctccATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAg                                                                                                                                                                                                                      <  1:60280/125‑1 (MQ=255)                                                                                                                                                 tcctccATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAg                                                                                                                                                                                                                      >  2:60280/1‑125 (MQ=255)                                                                                                                                                    tccATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCATAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTg                                                                                                                                           <  2:694711/197‑1 (MQ=255)                                                                                                                                                    tccATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTg                                                                                                                                           >  1:694711/1‑197 (MQ=255)                                                                                                                                                    cccaaaaaaTGCCCCTCCGT‑ACCTTCAATC‑GTGCAACAGAATGTGAAAACGTCAATACAGCCAACCTGGATTTACACCAACGGTTAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTAATTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGATTAATATTAATCAACATTTGGTGAGTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAg                                                                                     <  2:619870/246‑1 (MQ=255)                                                                                                                                                     ccATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAAc                                                                                                                                                                                                                               <  2:148807/112‑1 (MQ=255)                                                                                                                                                     ccATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAAc                                                                                                                                                                                                                               >  1:148807/1‑112 (MQ=255)                                                                                                                                                       aTAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGa                                                                                  <  1:545125/251‑1 (MQ=255)                                                                                                                                                         aaaaTGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATGAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTTGATATCTGAGCGAtt                                                                                <  2:338520/251‑1 (MQ=255)                                                                                                                                                            aTGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAAt                                                                             <  1:140406/251‑1 (MQ=255)                                                                                                                                                            aTGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAAt                                                                             <  1:735398/251‑1 (MQ=255)                                                                                                                                                                   tC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATg                                                                       >  1:378512/1‑250 (MQ=255)                                                                                                                                                                   tC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATg                                                                       >  1:432810/1‑250 (MQ=255)                                                                                                                                                                             aCAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCAACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGATGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTaaa                                                              >  2:113224/1‑250 (MQ=255)                                                                                                                                                                               aaTCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAAc                                                            >  2:173015/1‑250 (MQ=255)                                                                                                                                                                                    gT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAg                                                      <  1:681966/251‑1 (MQ=255)                                                                                                                                                                                      ccaaaataaattgAAAACGTCAATACAGACAACCGGGATTTACAACAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTACCAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGAACAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTTGATATCTGAGAGATTAATACCATGAACGCTAAAACGCACAGc                                                     <  2:726784/240‑1 (MQ=255)                                                                                                                                                                                          cAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGAGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGa                                                  >  2:799258/1‑250 (MQ=255)                                                                                                                                                                                             aaTGTTAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCAGAAACAAAGATTAAAAAAACTTCAAACAGCTATTTTCAGCAGAGAAATTTGTTCTACTACAACATGACCAGAACAATCAGCGTAATATGTAGCAACATTTGGTGATTAAGTTTTAGGCATTTAATGAATAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACTAGGAACTCTAAAACGCACAGCGGAAAAt                                              <  2:835601/251‑1 (MQ=255)                                                                                                                                                                                                  gAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGAAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACCTTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGcc                                           >  2:761253/1‑249 (MQ=255)                                                                                                                                                                                                        gTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGgccag                                   <  1:607809/251‑1 (MQ=255)                                                                                                                                                                                                                aGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGt                           <  1:209061/251‑1 (MQ=255)                                                                                                                                                                                                                   cAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAAc                        <  2:330358/251‑1 (MQ=255)                                                                                                                                                                                                                      ccgtgttttACAACAACTGTGATAAACCACAAACAAAGTTTAAAAAAACTTCAAACATCTCTTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAAGATTGAGCAACATTTGGTGATTAAGTTTTATTCATTTAATGAAAAAAAGCTGAGGAAAAGGTGGATAACTGAGCGATTAATACCATGAACGCGAAAACGCACAGCGGAAAACGCCAGGGCCAGCAATAAGTAACGCa                     <  2:486187/245‑1 (MQ=255)                                                                                                                                                                                                                        gggATTTACTCCAACGGTGAGAATCCTCAAAAAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGATACTCCAACATGTCCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTACTACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCaaa                   <  2:627214/251‑1 (MQ=255)                                                                                                                                                                                                                         ggATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCaaaa                  <  1:772098/251‑1 (MQ=255)                                                                                                                                                                                                                         ggATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCaaa                   >  1:180756/1‑250 (MQ=255)                                                                                                                                                                                                                               acaacAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGAt            <  1:723421/247‑1 (MQ=255)                                                                                                                                                                                                                                   cAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTaa        <  1:146540/251‑1 (MQ=255)                                                                                                                                                                                                                                   cAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGGGGATATCTGAGCGATTAATACCATGAACGCTAAAACACACAGCGGAAAATGCCAGGGCCAGCCATAAGTATCGCAAAAAAAGATTTaa        <  2:37933/251‑1 (MQ=255)                                                                                                                                                                                                                                     aCGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGTTTAATATTTAGCAACATGTGGTTATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGAGTAATACCATTAACGCTAAAACGCACAGCGTAAAATGCCAGGGCCAGCCATAAGTAACTCAAAAAAAGATTTAAGc      <  2:837919/251‑1 (MQ=255)                                                                                                                                                                                                                                      cGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGc      >  1:483627/1‑250 (MQ=255)                                                                                                                                                                                                                                      cGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCa     <  1:459456/251‑1 (MQ=255)                                                                                                                                                                                                                                          gagaATTCACAAACAAAGATTAAAAAAACATCAAACAGCTATTTGCAGGAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAATGTGGATATCTGAGCTATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAat  <  1:851283/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               TTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTC‑GTGACCTACAATCTGT‑CAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAAT  >  NC_000913/3707705‑3708185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑