Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	246,747	G→T	S12S (TCG→TCT)	yafL →	NlpC/P60 family protein YafL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	246,747	0	G	T	100.0%	168.1 / NA	53	S12S (TCG→TCT)	yafL	NlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/25);  total (28/25)

TGGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCC  >  NC_000913/246503‑246986                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                tGGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGAtt                                                                                                                                                                                                                                            >  1:269798/1‑250 (MQ=255)  ggggTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTAc                                                                                                                                                                                                                                          <  1:655514/251‑1 (MQ=255)     gTATCTCCTGGGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTAGTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGt                                                                                                                                                                                                                                       <  1:320815/251‑1 (MQ=255)       aTCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGCCAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGTGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATTTCCTTGCCGTCGATTCAATCCTTTGTCTTGTCTGGATTACTGTTg                                                                                                                                                                                                                                     >  2:606538/1‑251 (MQ=255)                gCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGAtttgtttg                                                                                                                                                                                                                            <  1:631578/251‑1 (MQ=255)                gCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGAtttgttt                                                                                                                                                                                                                             <  1:380285/250‑1 (MQ=255)                   gTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCg                                                                                                                                                                                                                         <  1:626523/251‑1 (MQ=255)                          tattCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTAGTTTGCCGTTttct                                                                                                                                                                                                                   <  1:773382/250‑1 (MQ=255)                           attCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTttcttc                                                                                                                                                                                                                 <  1:704144/251‑1 (MQ=255)                              cAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCAt                                                                                                                                                                                                               <  1:507098/250‑1 (MQ=255)                                  attaaTTGTAGCGCAAATTAGCAAAAACGACAATCCATGATTAATTTAATAATCGGATGTTTTTACATAATTAACTGAAATATATATTTTAATGTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGTATCCACTGCATCATTAATGCATGACCAGCAATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGc                                                                                                                                                                                                           <  2:781459/247‑1 (MQ=255)                                           aGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACCCTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGccacc                                                                                                                                                                                                 <  1:65830/251‑1 (MQ=255)                                               cAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACcacat                                                                                                                                                                                             >  1:484524/1‑251 (MQ=255)                                                    gAGCAACAGCGACAATCCATGAGTAATTTGATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTAGCCGTTTTCTTCATTTGCCAGCGCCACcacatcacat                                                                                                                                                                                        <  1:455632/251‑1 (MQ=255)                                                      gCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACatat                                                                                                                                                                                      <  2:177863/251‑1 (MQ=255)                                                                   tCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTAAGTATGTCCTTGCCGTCGATTCCATCCTTTGTCTTGTCTGGCTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTAc                                                                                                                                                                         >  2:776535/1‑251 (MQ=255)                                                                    ccATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTAc                                                                                                                                                                         <  1:838518/250‑1 (MQ=255)                                                                    ccATGAGGAATTTAATAAACGGATGTTTTTACATAAGTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTTTGCAGAAACTATCCGTGATACACTGCAACATTAAACAATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATTCTTTGTATTGCCTGGATTACTGTTGATTTGTTTGCCGTTTTCTGCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTAc                                                                                                                                                                         <  2:26690/250‑1 (MQ=255)                                                                                   taaACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCgg                                                                                                                                                          >  1:199223/1‑250 (MQ=255)                                                                                      aCGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGc                                                                                                                                                      >  1:850702/1‑251 (MQ=255)                                                                                           tGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATTGTTTATTAAGTTTGTTCAGAAACGATCCGGGATAAACTGCAAAATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGGTTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAAc                                                                                                                                                 >  2:531685/1‑251 (MQ=255)                                                                                                          aaCTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTttatt                                                                                                                                   <  1:74612/250‑1 (MQ=255)                                                                                                                   atatatTTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGGTTATTAAAACAGTAc                                                                                                                         <  1:680002/251‑1 (MQ=255)                                                                                                                   atatatGTTAATTTTATAA‑TAATGGTTTATTCAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCTAAACCGTGCGCGTTTATTAAAACAGTAc                                                                                                                         >  2:785193/1‑251 (MQ=255)                                                                                                                    tatatGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTACAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGTTTTGTTGTCCGTTTTCTTCATTTGCCAGCGCCACCACATCTCATATCTCTTTCAGCTACGCCTCCCGCCATCGGATGCAAAACCGTGCCGGTTTATTAAAACAGtccc                                                                                                                        >  2:242034/1‑248 (MQ=255)                                                                                                                         gTTAATTT‑ATAAATAATGTTTTTTTAAGTTTTTCCAGAACCGTTCTGGTATCCACTTCAACATTAAAGCATTACCTGCCATTATTTAGTATTTCCTTGCCGTCGATTCCATCCTTTTTATTGTCTGGATTACTGTTGATTTGTTTGCCTTTTTCTGCATTTGCCAGCGCCACCACATCACATACCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACt                                                                                                                   <  2:807911/251‑1 (MQ=255)                                                                                                                            aaTTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAACCGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGGATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCCCCACATCACATATCTCTTTCATCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCAt                                                                                                                <  1:632716/251‑1 (MQ=255)                                                                                                                                  ataa‑taATGGTTTATAAAGTTTTTTAAGATACGATCCGGTATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCTTCGATTCCAGCCTTTGTATGGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTCCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaa                                                                                                          <  1:496958/251‑1 (MQ=255)                                                                                                                                       taatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGAAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTACTCTGAAAAAGc                                                                                                      >  1:704933/1‑251 (MQ=255)                                                                                                                                                      tttGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACTTATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACGCATCTGAAAAAGCGGGCCAGCTATATtg                                                                                       >  1:694181/1‑251 (MQ=255)                                                                                                                                                        tGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATtgtg                                                                                     >  1:282735/1‑251 (MQ=255)                                                                                                                                                             aGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAACCCGTGCGCGTTTATTCAAACAGTACCCAACTCATCTGAAAAAGCAGGCCCGCTATATTGTGGAAgg                                                                                >  2:778646/1‑251 (MQ=255)                                                                                                                                                               aaaCGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGc                                                                               >  1:679071/1‑250 (MQ=255)                                                                                                                                                                  cGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGc                                                                                                                                                                                                           >  1:630421/1‑123 (MQ=255)                                                                                                                                                                  cGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGc                                                                                                                                                                                                           <  2:630421/123‑1 (MQ=255)                                                                                                                                                                    aTCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTACATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCAGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAACCTCATCTGAAAAACCAGGCCAGCTATATTGTGGAAGGCAATGcc                                                                         >  2:873381/1‑251 (MQ=255)                                                                                                                                                                        gggATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGAGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGaa                                                                      >  2:425201/1‑250 (MQ=255)                                                                                                                                                                        gggATACACTGCAACATAAAAGCATGACCAGCCATTATGGAGTATGTCCTGGCCGTCGATGCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCTTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTGCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGaaa                                                                     <  1:466543/251‑1 (MQ=255)                                                                                                                                                                           aTACACTGCAACATAACAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGc                                                                   >  1:699612/1‑250 (MQ=255)                                                                                                                                                                                         tAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGc                                                    >  1:740864/1‑251 (MQ=255)                                                                                                                                                                                           aaGCATGACCAGCCATTATTGAGGATGTCCTTGCCGTCGATTCCATCCTTTGTACTGTCTGGATTACTGATGATTTGTTTGCCGTTTTCTTCATTTGACAGCGCCACTACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAACCCCGTACGCGTTTATTAAAACAGTACAAACCTCATCTGACAAAGCAGGCCAGCTATCTTGTGGAAGGAACTGCCGAAAGAAAAAGGacgcaccacc                                                   >  2:799602/1‑244 (MQ=255)                                                                                                                                                                                            aGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTCCGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCATACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAACGCAAAAGGCCGCTACGcc                                                   >  1:639654/1‑249 (MQ=255)                                                                                                                                                                                                            attGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAg                                  <  1:400276/250‑1 (MQ=255)                                                                                                                                                                                                               gAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGACAGCACAACCGGGAGCAGATa                               <  1:661082/250‑1 (MQ=255)                                                                                                                                                                                                                aGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATaaa                             <  1:425201/251‑1 (MQ=255)                                                                                                                                                                                                                           gCCGTCGATTCCCTCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGATTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTCTATTGTGGAAGGCAATGCCGAAAGCAAAAGAGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATcc                    >  1:514508/1‑249 (MQ=255)                                                                                                                                                                                                                           gCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGAACAACCGGGAGCAGATAAAACAGCATcc                    >  1:414049/1‑249 (MQ=255)                                                                                                                                                                                                                             cGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGa                 >  1:512185/1‑250 (MQ=255)                                                                                                                                                                                                                                 gATTCCATCCTTTGTCTTGACTGGATTACTGTTGAGTTGTTTGTCGTTTTCTTCATTTGCCTGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTCAAACAGTACCAAACTCATATGAAAAAGCAGGCCAGCTATATTATGGAAGGCAATGCCGAACGCAAAAAGGAGCTACACCAACCTAACCGGGAGACGATAAAACAGCATCCAGAATgtt            >  2:448168/1‑249 (MQ=255)                                                                                                                                                                                                                                 gATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCGGATAAAACAGCATCCAGAATGGt            >  1:299228/1‑251 (MQ=255)                                                                                                                                                                                                                                   ttCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGttt          >  1:670387/1‑251 (MQ=255)                                                                                                                                                                                                                                      cATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCt       >  1:241480/1‑251 (MQ=255)                                                                                                                                                                                                                                            ttGTATTGTCTGGATTACTGGTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTcc  <  1:482178/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                TGGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAA‑TAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCC  >  NC_000913/246503‑246986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑