| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 91,916 | C→T | Y168Y (TAC→TAT) | ftsI → | peptidoglycan DD‑transpeptidase FtsI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 91,916 | 0 | C | T | 96.8% | 279.6 / 0.4 | 97 | Y168Y (TAC→TAT) | ftsI | peptidoglycan DD‑transpeptidase FtsI |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (49/43); total (51/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01 | |||||||||||
AAGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAAC > NC_000913/91678‑92162 | atgggatttgggcctcccgtaaaGATGTGCATGACGTCGACGGGTTCAGCGCCGTTGACCGCTGGAAGGCTCTGGCGAACGCTCACAATATTCCGCTGGCGCTGCTTTCAGCCCGCATTAACTCCACCCCTATATGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGTTCTACACCAAAAAACTTAATCTGCCGGGGATCCATCTGCGTGAAGAGTTTCGCCGTTACTATCCGTCCGGc < 2:347337/231‑1 (MQ=255) gCGATTTGGGCTGACCCGAAAGACGGGCATGCCGCGGGGGGTATCAGCGTCGGTGACCGCTGGAAGGCCCTGGCAAACGCGCTCAATATTCCGTTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCTTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGa < 2:246052/251‑1 (MQ=255) aTTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAgg > 1:341963/1‑250 (MQ=255) cGAAAGAAGTGCAGGAAGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTACAGCCCGCATTACCGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTc < 2:666046/251‑1 (MQ=255) gAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGAACAGGTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAACAAACTGAAAATGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCAACTc > 1:509325/1‑250 (MQ=255) gaagTGCAGGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGGGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCAttgg < 2:759746/251‑4 (MQ=255) aagTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCGCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGc < 1:36981/251‑1 (MQ=255) ggCATGACGCTTGCGGTATCAGCGTCGTTGACCGCCGAAAGGCGCGGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACAGGGCGGTTTACATCAAAAAACGGAAACGGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTAGTATCCATCCGGCGAAGTGACTGCGCACCTCATTGGCttt < 2:851377/250‑1 (MQ=255) cATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTAc > 1:198458/1‑251 (MQ=255) gACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTaa < 1:373661/251‑1 (MQ=255) gACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCCCAATATTCCGCTGGATCAGCTTTCCGCCCGCATTAACGCCACCCCGAAAGGGCGCTTTTTTTATCTGGCGCGTCAGGTGAACCCTGCCATGGCGGACTACATCAAAAAAACGAAAATGCCGGGGATTCATCTGCGTGAAGAATCTCCCCGTTTTTATCCATCCGGCGACGTGCCTGCTCACCTCACTGGCTTTCCTac > 2:233825/1‑250 (MQ=255) aCGCTGGCGGTATCAACGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCCATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAAACCGCAAGGGCGCTTTATTTTTCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAACACTGAAAATGCCGGGGATTCATCTTCGGGAAGAATCTCGGCGTTATTATCCATCAGGCGAAGTGACAGCTCACCCCATTGGCTTTACtca > 2:786564/1‑248 (MQ=255) gCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAAAGCGCTCCCTATTCCGCTGGATCAGCTTTCAGCCCGCATTAACCCCAACCCGAAAGGGCGCTTTTTTTTTCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTAAATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGAGGCTGCTCACCTCATTGGCTTTACTaaagt > 1:56339/1‑248 (MQ=255) gggcggtatacacgcaggttgccGCTGGAAGGCGCGGGCTAACGTGTGTAATTTTCCGCTGGATCAGCTTTCAGCCCGCAGTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTTAACCCGGACATGGAGGACTACATCAAAAATCTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCGCGCCGTTATTATCCATCCGGCGAAGTGTCTGCTCACCCCATTGGCTTTTCTAACGTCg < 2:816369/230‑1 (MQ=255) ggCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGa < 2:217803/251‑1 (MQ=255) ggTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAg < 1:215192/251‑1 (MQ=255) cggggtggttaCCGCTGGAAGGCGTTGGCTAACGCGTTCAATAATCCGCTGGATCAGCTGTCAGCCCGCATTAACGACAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTTACATGGCGGATTACATCAAAAAACTGAAACTGCCGGGGATTCATCGGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCGCATTGGCTTTACTAACGGCGATAGTCAAgg < 2:159784/241‑1 (MQ=255) cGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAggg > 1:160689/1‑250 (MQ=255) tCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGAtt > 1:255397/1‑251 (MQ=255) cgggTGACCTCTGGATGGCTCTGGCTAACGCGCTCTATATTCCGCTGGAGCCGCTTTCAGCACGCATTAACGCCAACCCGAAAGGGTGTTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGTGGACTACAGCAAAAATCTTAAACTGCCGGGGATTCATCTGCTTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCACCCCTCATTGGCTTTACTACCGTCTATAGTCAAGGGAtt < 2:695986/249‑1 (MQ=255) tGACCGCTGGAAGGCGCTGGCTCACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTAACCTAATTGGCTTTACTAACGTCGATAGTCAAGGGATTgggg > 2:277237/1‑248 (MQ=255) gACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAggg > 1:866641/1‑251 (MQ=255) cGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCATGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAGGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGCTTGAGGGCGt > 1:384587/1‑251 (MQ=255) ccgggAAGGCGCTGGCTCACGCGCTCAAAATTCCGCTGGAGCAGCTTTCAGCCCGCATTAACGCCAACCCGAGAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGtt < 2:704321/248‑1 (MQ=255) ggAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTg > 1:375465/1‑249 (MQ=255) cgcTGCCTAACGCGCACAATATTCCGCTGGATCAGCTTTCCGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATATGGCGCGTCAGGTGAACCCTGCCATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGATATTATCCATCCGGCGAAGTGACTGCTCACCTCATAGGCTTTACTGACGTCCATAGTCACGGGATTGAGGGCGTTGAGAAGAGt > 2:191602/1‑251 (MQ=255) gcTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGtt < 1:22419/251‑1 (MQ=255) gcTGGATAACGCGCTAAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGACACGGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGtt < 2:179858/251‑1 (MQ=255) ggCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCTCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGttt < 1:114204/249‑1 (MQ=255) ggCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCg > 1:134943/1‑251 (MQ=255) gcgcTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATg < 1:589749/251‑1 (MQ=255) cgcTCAATATTCCGCTGGATCAGCTTTAAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGACGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAGGGGATTGAGGGCGTTGGGAAGAGTTTCACTAAATgg > 2:634193/1‑251 (MQ=255) cAATATTTCGCTGGCTCAGCTTTCAGCCCGCATTAACGCCAACCCGAACGGGCGCTTTATTTCTCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAACAACCTGACACTGCCGGGGATTAATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCACGGGCTTGAGGGCGTTGAGAAGAGTTTCGTTTAATGGCtt > 2:227042/1‑250 (MQ=255) cAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGCTAGTCAAGGGATTGAGGGAGTTGAGAAGAGTTTCGATAAATGGCtt > 2:798557/1‑250 (MQ=255) cAATATTCCGCTGGATCAGCTTTCAGCCCCCTTTAACCCCAACCCGAAAGGGCTCTTTATTTATCTGGCGCGTCAGGTGAACCCTTACAGGGCGGACTACATAAAAAATCTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCAGCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGc < 2:629548/248‑1 (MQ=255) aatagcccGCTGGATTAGTTTTCCGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGGAAACCCTGACATGGCGGACAACATCAAAAAACTGAAACTGCCGGGGATTAATCTTCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTAc < 2:13507/245‑1 (MQ=255) aaTATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTAc > 1:367280/1‑251 (MQ=255) atTCCGCTGGCTCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCgg < 2:486857/251‑1 (MQ=255) atTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCgg < 2:866641/251‑1 (MQ=255) cTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAgcg > 1:361966/1‑251 (MQ=255) tttCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAAGGCGTTGGGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGGgcgc > 2:327548/1‑251 (MQ=255) cAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCAtt < 2:341963/251‑1 (MQ=255) gCCCGCATTAACGCAAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGACGTTATTATCCATCCGGCGAAGTGACTGCTCACATCATTGGCTTTACTAACGTCGATAGTAAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑GGAGCGCATtgg > 2:191847/1‑250 (MQ=255) cGCATTCACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGGGAACCCTGAAATAGAGGACTACATCAAAAAACTGAAACAGCCGGAGATTCATCTGCGTGACGAATCTCGCCGTTATTATCCATCCGGCGAATAGACAGCTCACCTCATTGGCTATACTAACGTCGATAGTCAAGGGTTTGAGGGCGTTGAGAAGACATTCGATAAATTACTTACCGTGCAACCGGGATG‑GCGCATTTTGCg > 2:536776/1‑251 (MQ=255) cATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGAGTCATCTGCGTGAAGAATCCCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGt < 2:378625/250‑1 (MQ=255) gCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACCGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCg > 1:234880/1‑251 (MQ=255) cAACCCGAAAGGGCGCTTTAGTTACCTGGCGCGTCAGGTGACCCCTGACATGGCGGACTACATCAAAAAACTTAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGTGCATTGTGCGTAAAGACCGCt < 2:198458/251‑1 (MQ=255) aaatggCGCTTTATTTCTCTGGAGCGTCAGTGGAACCCTGACATGGCGGACTTCATCAAAAAACGGAAACAGCCGGGGAGTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCTCCTCATGGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTcg < 2:338933/247‑1 (MQ=255) aaaGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACCTGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTcg < 2:232343/251‑1 (MQ=255) aGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTcgcg > 1:454961/1‑251 (MQ=255) gggCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGt < 1:370090/251‑1 (MQ=255) ggCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATtg < 2:668078/225‑1 (MQ=255) ggCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATtg > 1:668078/1‑225 (MQ=255) gcgcTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTa > 1:631599/1‑250 (MQ=255) cgcTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAAt < 1:633203/251‑1 (MQ=255) gcTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGGGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑GGCGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAAtt > 2:81204/1‑251 (MQ=255) cTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTg > 2:138702/1‑251 (MQ=255) tatttatCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGaa < 1:569923/250‑1 (MQ=255) ttatCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGAGTAAAGACCGCTATGGTCGCGTAATTGAAGat > 1:357194/1‑250 (MQ=255) atCTGGCGCGTCAGGGGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAACGACCGCTATGGTCGCGTAATTGAAGATAtt > 2:562447/1‑251 (MQ=255) ggctcgTCAGGTGATCCCTGCCATGGGTGACTAAATCAAAAAATGGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGCAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATttc < 2:102260/245‑1 (MQ=255) ggCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATttctt < 1:255272/251‑1 (MQ=255) ggCGCGTCAGGTGAACCCCGACATGGCGGACTACATCAAAAAACTTAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGACGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAAGTGAAGATATttct < 2:58181/250‑1 (MQ=255) cgcttCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGGGCATAAAGACCGCTATGGGCGCGTAATTGAAGATATTtcttct < 1:738130/247‑1 (MQ=255) cgTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGCCTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGCTTGAGGGCGTCGAGAAGAGTTTCGATAAATGGCTTACCGAGCAGCCGGG‑CGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGGAGATAtttattatac > 2:526205/1‑244 (MQ=255) cgTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTAc > 2:502056/1‑251 (MQ=255) cgggTGAACCCTGACATGGGGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAAGCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTGGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGa < 2:258085/249‑1 (MQ=255) ggTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGAc > 1:623420/1‑250 (MQ=255) ggAACCCTGACATGGCGGACTACATAAAAAAACTGAAACTTCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑Gc < 2:239866/250‑1 (MQ=255) cccTGACATGGCGGACTACAGCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAgg < 1:357197/251‑1 (MQ=255) aTGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGACTCTCGCCGTTATTATCCATACGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGCTAAATGGCTTACCGGGCGGCCGGG‑TGAGCGCGTTGTGCGTAAAGACCGCTATGGTCGCGTAATTGCAGCTATTTCTTCTACT‑ACAAGCCAGGctgagcc > 2:484985/1‑245 (MQ=255) ggCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGcac < 1:233825/250‑1 (MQ=255) aCATCAAAAAACTGAAACTTCCGGGGATCCATCTGCGTGAAGAATCTCGCCTTTATTATCCATCCGGCGAAGTGAATGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGTCCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGgcg < 2:456310/251‑1 (MQ=255) aaaaaaCTGAAACTGCCGGGGATTCATCTTCGTGAAGAATCTCGCCGTTATTATCCATCCGGAGAAGTGACTGCTCACCTCATTGGCTTTACTAGCGTCGATAGTCCAGGGATTGAGGGCGTTGAGAAGAGATTCGATAAATGGCTTACCGGGCAGCCGGG‑TGCGCGCATTGTACGTAAAGACCGCTATGGTAGCGTAAATGAAGATATTTCTTCTACTGACA‑GCCCGGCTGCGCACACCCTAGCGCTggg > 2:244445/1‑249 (MQ=255) aaaaaaCTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCTTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTg > 1:189080/1‑249 (MQ=255) aaaaCTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTa < 1:487884/251‑1 (MQ=255) atgaaactgCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTATTAACGTCGATCGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCAGCGCCCAACCTGGCGCTGAGTATtgt > 2:29622/2‑250 (MQ=255) cgtaacatccGGGGATCCATCTGCGTAAAGCACCTCTCCGTTATTATCCAGCCGGCGAAGTGACTGCGCACCTCATTGGCTTTAAGAACGTCGATAGTCAAGGGATTGTGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TTAGCTCAGTGTGCGTAAAGACCGCTATGTTCGCGTAATTGAAGATATTTCTACTACTGACA‑GCCAGGCTGCGCACTACCTGGCGCTGAGTAAtgat < 2:715392/243‑1 (MQ=255) gaaactgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGTCTGCTCACCTCATTGGCTTTACTAACGGCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTTACA‑GCCAGGCTGCGCACAACCTGTCGCTGAGTATtgat < 2:375465/250‑1 (MQ=255) tgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCACGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGc > 1:166075/1‑251 (MQ=255) tgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGc > 1:446832/1‑251 (MQ=255) cgCCGGGGATTCATTTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGc < 2:272883/250‑1 (MQ=255) gCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATGGATGAACGcc > 1:452190/1‑251 (MQ=255) cGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTg > 1:675989/1‑251 (MQ=255) ggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAg < 1:209783/251‑1 (MQ=255) gATTCATCTGCGTGAAGAACCTCGCCGTTAGTATCCATCCGGCGAAGTTCCTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGGGCGTAAAGTCCGCTATGGTCGCGTAATTGAAGATATTTCTTCTCCTGACA‑GCCAGGCTGCTCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAgg < 2:208198/251‑1 (MQ=255) aTTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGgc > 1:214119/1‑251 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGgcg > 2:418774/1‑251 (MQ=255) tCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACGGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGAACGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGCGTATTGATGACCGCCGGCAGgcgc > 2:751869/1‑251 (MQ=255) tGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGtt > 1:350422/1‑251 (MQ=255) gTGAAGAGTCTCGCCGTTACTATACGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTAt > 2:181024/1‑251 (MQ=255) agaatctcGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATcgcg > 2:207958/5‑251 (MQ=255) atctcGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGCAGAGTTTCAATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGGGCGTAAAGACCGCTATGGTCGCGTAATTGAGGATATTTCTTCTACTGACA‑GCCCGGCTGCGCACAACCTGGCGCTGAGTATTGCTGAACGCCTGCAGGCCCTGGTTTATCGCGAAc > 2:826445/2‑251 (MQ=255) cggcGTTATTATCCATCCGGCGAAGTGGCTGTGCACCTCATTGGTGTTACTAACGTCGATAGTCAAGGGATCGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCAGAGGATTGATGAACGCCTGCAGGCGCTGGTTTATCTCGAACTGaa < 2:239942/248‑1 (MQ=255) gCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGaac > 1:627111/1‑251 (MQ=255) gCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGaac > 1:771666/1‑251 (MQ=255) gTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCTGCGCACAACCTGGCGCTGCGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGaccaat > 2:818128/1‑250 (MQ=255) | AAGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGG‑TGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACA‑GCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAAC > NC_000913/91678‑92162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |