breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 147,213 2 bp→TT coding (246‑247/903 nt) rpnC → recombination‑promoting nuclease RpnC
RA 150,501 G→A I151I (ATC→ATT yadC ← fimbrial tip‑adhesin YadC
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
JC JC 295,738 IS2 (–) +5 bp coding (1355‑1359/1401 nt) intF ← putative phage integrase
RA 299,490 T→C E482G (GAA→GGA)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 350,292 G→A R94H (CGT→CAT)  prpC → 2‑methylcitrate synthase
RA 364,170 2 bp→AA coding (2135‑2136/3075 nt) lacZ ← beta‑galactosidase
RA 447,538 A→G F56F (TTT→TTC cyoE ← heme O synthase
RA 460,060 C→T I391I (ATC→ATT lon → Lon protease
RA 471,899 C→T Q422* (CAA→TAA)  mdlB → ABC transporter family protein MdlB
RA 475,884 C→T T169I (ACC→ATC)  ybaY → PF09619 family lipoprotein YbaY
RA 483,878 G→A Q176* (CAG→TAG)  acrB ← multidrug efflux pump RND permease AcrB
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
RA 562,201 C→T G835G (GGC→GGT sfmD → putative fimbrial usher protein SfmD
RA 579,811 C→T intergenic (‑217/‑69) ybcV ← / → ybcW DUF1398 domain‑containing protein YbcV/uncharacterized protein YbcW
RA 584,506 C→T intergenic (+76/+174) appY → / ← ompT DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT
JC JC 585,707 IS2 (+) +5 bp intergenic (‑74/+436) ompT ← / ← envY omptin family outer membrane protease OmpT/DNA‑binding transcriptional activator EnvY
RA 789,579 2 bp→AA coding (400‑401/1149 nt) galK ← galactokinase
JC JC 926,188 IS2 (–) +5 bp intergenic (‑217/+33) serW ← / ← infA tRNA‑Ser/translation initiation factor IF‑1
RA 928,622 G→A L192L (CTG→TTG)  cydC ← glutathione/L‑cysteine ABC exporter subunit CydC
RA 1,018,847 G→A Q121Q (CAG→CAA matP → macrodomain Ter protein
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
JC JC 1,228,095 IS2 (+) +5 bp coding (17‑21/660 nt) ycgM → putative isomerase/hydrolase
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
JC JC 1,399,590 IS5 (+) +4 bp intergenic (‑64/+128) fnr ← / ← ogt DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,477,804 C→T L62F (CTT→TTT)  ynbA → CDP‑alcohol phosphatidyltransferase domain‑containing protein YnbA
JC JC 1,590,834 IS2 (–) +5 bp intergenic (‑833/+16) ydeT ← / ← hipA fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA
RA 1,605,953 G→A Q301Q (CAG→CAA lsrB → Autoinducer‑2 ABC transporter periplasmic binding protein
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,656,658 G→A A159T (GCC→ACC)  speG → spermidine N‑acetyltransferase
JC JC 1,671,143 IS2 (+) +5 bp intergenic (+191/‑229) ynfM → / → asr putative transporter YnfM/periplasmic chaperone Asr
RA 1,869,314 G→A S120S (TCG→TCA yeaH → DUF444 domain‑containing protein YeaH
JC JC 1,908,815 IS2 (+) +5 bp intergenic (‑49/‑106) mgrB ← / → yobH PhoQ kinase inhibitor/uncharacterized protein YobH
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,039,555 A→G L26L (TTA→TTG msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,061,989 T→C intergenic (‑56/‑271) nac ← / → asnV DNA‑binding transcriptional dual regulator Nac/tRNA‑Asn
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,174,844 IS5 (+) +4 bp coding (203‑206/453 nt) gatA ← galactitol‑specific PTS enzyme IIA component
RA 2,268,985 G→A Q44Q (CAG→CAA lpxT → lipid A‑core phosphotransferase
JC JC 2,317,225 IS2 (–) +5 bp coding (2648‑2652/2850 nt) rcsC ← histidine kinase RcsC
RA 2,422,263 G→A G186S (GGC→AGC)  yfcH → epimerase family protein YfcH
JC JC 2,448,586 IS5 (–) +4 bp intergenic (‑146/‑17) prmB ← / → smrB ribosomal protein L3 N(5)‑glutamine methyltransferase/putative endonuclease SmrB
JC JC 2,470,820 IS2 (+) +5 bp intergenic (‑177/+253) yodE ← / ← yfdK toxic protein YodE/putative tail fiber assembly protein YfdK
RA 2,474,885 G→C intergenic (+29/‑99) yfdQ → / → yfdR DUF2303 domain‑containing protein YfdQ/5'‑deoxynucleotidase
RA 2,577,987 C→A G131C (GGC→TGC)  maeB ← malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
RA 2,648,617 T→C D1224G (GAT→GGT)  yfhM ← alpha2‑macroglobulin
RA 2,656,129 C→T G77D (GGT→GAT)  pepB ← aminopeptidase B
RA 2,681,500 G→A P416S (CCT→TCT)  yphG ← DUF5107 domain‑containing protein YphG
JC JC 2,698,402 IS30 (+) +2 bp coding (148‑149/636 nt) pgpC ← phosphatidylglycerophosphatase C
RA 2,731,034 G→A noncoding (124/1542 nt) rrsG ← 16S ribosomal RNA
RA 2,731,327 C→T intergenic (‑170/+273) rrsG ← / ← clpB 16S ribosomal RNA/chaperone protein ClpB
RA 2,751,849 G→A D19N (GAT→AAT)  recN → DNA repair protein RecN
RA 2,780,701 T→C N676D (AAT→GAT)  ypjA ← adhesin‑like autotransporter YpjA
RA 2,811,848 G→A S141N (AGC→AAC)  emrA → multidrug efflux pump membrane fusion protein EmrA
RA 2,823,589 G→A R61C (CGT→TGT)  recA ← DNA recombination/repair protein RecA
RA 2,866,783 G→A Q257* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,867,923 C→T G277G (GGG→GGA nlpD ← murein hydrolase activator NlpD
RA 2,873,316 T→C T8A (ACA→GCA)  ygbE ← DUF3561 domain‑containing inner membrane protein YgbE
RA 2,878,434 G→A intergenic (+816/+135) iap → / ← cas2 alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
RA 2,891,085 G→A P272S (CCG→TCG)  cysJ ← sulfite reductase, flavoprotein subunit
RA 2,903,727 G→A intergenic (+353/+1020) ygcE → / ← queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
RA 2,913,761 G→A L414L (CTG→TTG)  rlmD ← 23S rRNA m(5)U1939 methyltransferase
RA 2,930,364 G→A W263* (TGG→TGA sdaB → L‑serine deaminase II
RA 2,937,789 G→A A108T (GCA→ACA)  fucK → L‑fuculokinase
RA 3,017,470 C→T P471S (CCG→TCG)  ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,032,832 C→T R6C (CGC→TGC)  yqfG → uncharacterized protein YqfG
RA 3,036,781 G→A F442F (TTC→TTT recJ ← ssDNA‑specific exonuclease RecJ
RA 3,039,623 G→A H41Y (CAT→TAT)  xerD ← site‑specific recombinase
RA 3,041,451 G→A A47T (GCA→ACA)  ygfZ → folate‑binding protein
RA 3,065,497 G→A Q232Q (CAG→CAA scpC → propionyl‑CoA:succinate CoA transferase
RA 3,182,389 G→A P16S (CCA→TCA)  ygiD ← 4,5‑DOPA dioxygenase extradiol
RA 3,244,460 G→C H94Q (CAC→CAG uxaC ← D‑glucoronate/D‑galacturonate isomerase
JC JC 3,252,952 IS2 (–) +5 bp coding (42‑46/357 nt) yhaI → putative inner membrane protein
JC JC 3,262,484 IS5 (–) +4 bp coding (1174‑1177/1209 nt) tdcD ← propionate kinase
RA 3,263,710 C→T L436L (CTG→CTA tdcC ← threonine/serine:H(+) symporter
JC JC 3,361,732 IS5 (–) +4 bp coding (557‑560/765 nt) gltF → periplasmic protein GltF
RA 3,413,827 G→A intergenic (‑362/‑37) envR ← / → acrE DNA‑binding transcriptional repressor EnvR/multidrug efflux pump membrane fusion lipoprotein AcrE
RA 3,474,290 T→C K88R (AAA→AGA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,555,759 A→T Q892L (CAA→CTA)  malT → DNA‑binding transcriptional activator MalT
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,572,319 T→G E395A (GAA→GCA)  glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,633,668 T→A intergenic (‑1078/‑1173) yhiJ ← / → yhiM DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM
JC JC 3,725,990 IS5 (–) +4 bp coding (104‑107/996 nt) wecH → O‑acetyltransferase WecH
RA 3,797,329 C→T L128L (CTA→TTA)  waaL → O‑antigen ligase
MC JC 3,888,720 Δ6 bp intergenic (+211/‑5) tnaC → / → tnaA tnaAB operon leader peptide/tryptophanase
RA 3,957,805 G→A L6L (CTG→TTG)  ilvY ← DNA‑binding transcriptional dual regulator IlvY
RA 3,971,703 G→A E148E (GAG→GAA wecC → UDP‑N‑acetyl‑D‑mannosamine dehydrogenase
RA 3,977,463 G→A A396T (GCA→ACA)  wzxE → lipid IIIECA flippase
RA 4,004,180 G→A intergenic (‑2/+50) rarD ← / ← yigI putative transporter RarD/putative thioesterase YigI
RA 4,018,586 G→A L418L (CTG→CTA rmuC → putative recombination limiting protein RmuC
RA 4,149,535 C→T G244D (GGC→GAC)  eptC ← phosphoethanolamine transferase EptC
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,309,712 C→T W423* (TGG→TGA alsA ← D‑allose ABC transporter ATP binding subunit
RA 4,311,002 2 bp→GT intergenic (‑22/+104) alsA ← / ← alsB D‑allose ABC transporter ATP binding subunit/D‑allose ABC transporter periplasmic binding protein
RA 4,392,517 G→A noncoding (46/76 nt) glyX → tRNA‑Gly
RA 4,515,556 G→A S374S (AGC→AGT fecA ← ferric citrate outer membrane transporter
RA 4,521,151 C→T G290G (GGG→GGA yjhF ← putative transporter YjhF
JC JC 4,577,765 IS5 (–) +4 bp coding (191‑194/1047 nt) mcrC ← Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit
RA 4,578,759 G→A I193I (ATC→ATT
I193I (ATC→ATT
mcrB ←
mcrB ←
5‑methylcytosine‑specific restriction enzyme subunit McrB
5‑methylcytosine‑specific restriction enzyme subunit McrB
RA 4,624,587 G→A S68L (TCA→TTA)  ytjB ← putative inner membrane protein Smp

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 480924 481029 106 32 [0] [0] 21 tomB/acrB Hha toxicity modulator TomB/multidrug efflux pump RND permease AcrB
* * ÷ NC_000913 1978495 1979270 776 29 [0] [0] 22 insB5–insA5 insB5,insA5
* * ÷ NC_000913 2558699 2560127 1429 35 [0] [2] 3 intZ intZ
* * ÷ NC_000913 2560138 2560630 493 3 [2] [2] 3 [yffL] [yffL]
* * ÷ NC_000913 2560808 2565488 4681 3 [1] [2] 37 [yffL]–[eutA] [yffL],yffM,yffN,yffO,yffP,yffQ,yffR,yffS,[eutA]
* * ÷ NC_000913 3423907–3424231 3424424–3424238 8–518 3 [2] [2] 3 rrlD 23S ribosomal RNA
* * ÷ NC_000913 4215400 4216928 1529 30 [2] [2] 3 [aceB] [aceB]
* * ÷ NC_000913 4217634 4218626 993 3 [2] [2] 3 [aceA]–[aceK] [aceA],[aceK]
* * ÷ NC_000913 4218739 4221609 2871 3 [2] [2] 3 [aceK]–[arpA] [aceK],aceK‑int,[arpA]
* * ÷ NC_000913 4222422 4223855 1434 3 [2] [2] 3 [arpA]–[metH] [arpA],iclR,[metH]
* * ÷ NC_000913 4224643 4225518 876 3 [2] [2] 3 metH cobalamin‑dependent methionine synthase
* * ÷ NC_000913 4225730 4225951 222 3 [2] [2] 3 metH cobalamin‑dependent methionine synthase
* * ÷ NC_000913 4226302 4226579 278 3 [2] [2] 3 metH cobalamin‑dependent methionine synthase
* * ÷ NC_000913 4226758 4227755 998 3 [2] [2] 3 [metH]–[yjbB] [metH],[yjbB]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)21 (0.520) 21/496 0.7 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 481030 = 0 (0.000)intergenic (‑322/+224) tomB/acrB Hha toxicity modulator TomB/multidrug efflux pump RND permease AcrB
* ? NC_000913 = 382590NA (NA)32 (0.790) 28/496 0.4 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 480923 0 (0.000)intergenic (‑215/+331) tomB/acrB Hha toxicity modulator TomB/multidrug efflux pump RND permease AcrB
* ? NC_000913 = 56677449 (1.210)17 (0.420) 9/492 1.6 26.7% pseudogene (91/93 nt) renD protein RenD
?NC_000913 568036 = 45 (1.120)pseudogene (2/213 nt) renD protein RenD
* ? NC_000913 687851 =NA (NA)30 (0.740) 30/496 0.3 52.6% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 821414 27 (0.670)coding (622/714 nt) ybhM Bax1‑I family protein YbhM
* ? NC_000913 1207790 =3 (0.070)8 (0.210) 8/464 1.7 80.8% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 1 (0.030)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12078052 (0.050)13 (0.340) 13/464 1.1 90.1% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 1 (0.030)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)8 (0.200) 8/488 1.8 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)29 (0.710) 26/496 0.5 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)22 (0.540) 20/496 0.7 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 2288919 =NA (NA)28 (0.690) 28/496 0.4 93.3% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4215399 2 (0.050)intergenic (+190/‑79) metA/aceB homoserine O‑succinyltransferase/malate synthase A
* ? NC_000913 = 2290113NA (NA)28 (0.690) 26/496 0.5 56.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 3746186 = 22 (0.540)coding (93/987 nt) yiaO 2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ periplasmic binding protein
* ? NC_000913 = 25586980 (0.000)35 (0.890) 34/480 0.2 100% intergenic (‑19/‑160) eutB/intZ ethanolamine ammonia‑lyase subunit alpha/putative phage integrase IntZ
?NC_000913 2565489 = 0 (0.000)coding (1396/1404 nt) eutA ethanolamine ammonia‑lyase reactivase EutA
* ? NC_000913 2765412 =11 (0.270)18 (0.540) 16/408 0.7 76.5% intergenic (‑259/+101) abpA/yfjM anti‑bacteriophage protein/protein YfjM
?NC_000913 = 2765419 2 (0.060)intergenic (‑266/+94) abpA/yfjM anti‑bacteriophage protein/protein YfjM
* ? NC_000913 = 294746715 (0.370)5 (0.190) 5/330 1.7 38.5% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 6 (0.220)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 3042321 =32 (0.790)34 (0.950) 32/438 0.2 62.3% intergenic (+28/+168) ygfZ/yqfA folate‑binding protein/hemolysin‑III family protein YqfA
?NC_000913 = 3042439 13 (0.360)intergenic (+146/+50) ygfZ/yqfA folate‑binding protein/hemolysin‑III family protein YqfA
* ? NC_000913 3186096 =NA (NA)23 (0.570) 23/494 0.6 43.4% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4366042 30 (0.740)coding (732/1302 nt) dcuA C4‑dicarboxylate transporter DcuA
* ? NC_000913 = 3653230NA (NA)52 (1.280) 46/496 0.1 91.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4228848 = 5 (0.120)coding (1118/1632 nt) yjbB putative inorganic phosphate export protein YjbB
* ? NC_000913 4366038 =29 (0.710)23 (0.570) 22/496 0.6 44.2% coding (736/1302 nt) dcuA C4‑dicarboxylate transporter DcuA
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region