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breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 147,213 | 2 bp→TT | coding (246‑247/903 nt) | rpnC → | recombination‑promoting nuclease RpnC |
RA | 150,501 | G→A | I151I (ATC→ATT) | yadC ← | fimbrial tip‑adhesin YadC |
MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
JC JC | 295,738 | IS2 (–) +5 bp | coding (1355‑1359/1401 nt) | intF ← | putative phage integrase |
RA | 299,490 | T→C | E482G (GAA→GGA) | paoC ← | aldehyde dehydrogenase: molybdenum cofactor‑binding subunit |
RA | 350,292 | G→A | R94H (CGT→CAT) | prpC → | 2‑methylcitrate synthase |
RA | 364,170 | 2 bp→AA | coding (2135‑2136/3075 nt) | lacZ ← | beta‑galactosidase |
RA | 447,538 | A→G | F56F (TTT→TTC) | cyoE ← | heme O synthase |
RA | 460,060 | C→T | I391I (ATC→ATT) | lon → | Lon protease |
RA | 471,899 | C→T | Q422* (CAA→TAA) | mdlB → | ABC transporter family protein MdlB |
RA | 475,884 | C→T | T169I (ACC→ATC) | ybaY → | PF09619 family lipoprotein YbaY |
RA | 483,878 | G→A | Q176* (CAG→TAG) | acrB ← | multidrug efflux pump RND permease AcrB |
RA | 557,635 | A→T | L36Q (CTG→CAG) | folD ← | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase |
RA | 562,201 | C→T | G835G (GGC→GGT) | sfmD → | putative fimbrial usher protein SfmD |
RA | 579,811 | C→T | intergenic (‑217/‑69) | ybcV ← / → ybcW | DUF1398 domain‑containing protein YbcV/uncharacterized protein YbcW |
RA | 584,506 | C→T | intergenic (+76/+174) | appY → / ← ompT | DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT |
JC JC | 585,707 | IS2 (+) +5 bp | intergenic (‑74/+436) | ompT ← / ← envY | omptin family outer membrane protease OmpT/DNA‑binding transcriptional activator EnvY |
RA | 789,579 | 2 bp→AA | coding (400‑401/1149 nt) | galK ← | galactokinase |
JC JC | 926,188 | IS2 (–) +5 bp | intergenic (‑217/+33) | serW ← / ← infA | tRNA‑Ser/translation initiation factor IF‑1 |
RA | 928,622 | G→A | L192L (CTG→TTG) | cydC ← | glutathione/L‑cysteine ABC exporter subunit CydC |
RA | 1,018,847 | G→A | Q121Q (CAG→CAA) | matP → | macrodomain Ter protein |
JC JC | 1,105,376 | IS2 (–) +5 bp | intergenic (+83/‑34) | csgC → / → ymdA | curlin chaperone/uncharacterized protein YmdA |
JC JC | 1,228,095 | IS2 (+) +5 bp | coding (17‑21/660 nt) | ycgM → | putative isomerase/hydrolase |
RA | 1,337,394 | A→G | S522G (AGC→GGC) | acnA → | aconitate hydratase 1 |
JC JC | 1,399,590 | IS5 (+) +4 bp | intergenic (‑64/+128) | fnr ← / ← ogt | DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase |
RA | 1,477,804 | C→T | L62F (CTT→TTT) | ynbA → | CDP‑alcohol phosphatidyltransferase domain‑containing protein YnbA |
JC JC | 1,590,834 | IS2 (–) +5 bp | intergenic (‑833/+16) | ydeT ← / ← hipA | fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA |
RA | 1,605,953 | G→A | Q301Q (CAG→CAA) | lsrB → | Autoinducer‑2 ABC transporter periplasmic binding protein |
RA | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
RA | 1,656,658 | G→A | A159T (GCC→ACC) | speG → | spermidine N‑acetyltransferase |
JC JC | 1,671,143 | IS2 (+) +5 bp | intergenic (+191/‑229) | ynfM → / → asr | putative transporter YnfM/periplasmic chaperone Asr |
RA | 1,869,314 | G→A | S120S (TCG→TCA) | yeaH → | DUF444 domain‑containing protein YeaH |
JC JC | 1,908,815 | IS2 (+) +5 bp | intergenic (‑49/‑106) | mgrB ← / → yobH | PhoQ kinase inhibitor/uncharacterized protein YobH |
RA | 2,032,423 | G→A | V219V (GTC→GTT) | yedJ ← | putative HD superfamily phosphohydrolase YedJ |
RA | 2,039,555 | A→G | L26L (TTA→TTG) | msrP → | protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP |
RA | 2,061,989 | T→C | intergenic (‑56/‑271) | nac ← / → asnV | DNA‑binding transcriptional dual regulator Nac/tRNA‑Asn |
RA | 2,173,363 | Δ2 bp | intergenic (‑490/+918) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
JC JC | 2,174,844 | IS5 (+) +4 bp | coding (203‑206/453 nt) | gatA ← | galactitol‑specific PTS enzyme IIA component |
RA | 2,268,985 | G→A | Q44Q (CAG→CAA) | lpxT → | lipid A‑core phosphotransferase |
JC JC | 2,317,225 | IS2 (–) +5 bp | coding (2648‑2652/2850 nt) | rcsC ← | histidine kinase RcsC |
RA | 2,422,263 | G→A | G186S (GGC→AGC) | yfcH → | epimerase family protein YfcH |
JC JC | 2,448,586 | IS5 (–) +4 bp | intergenic (‑146/‑17) | prmB ← / → smrB | ribosomal protein L3 N(5)‑glutamine methyltransferase/putative endonuclease SmrB |
JC JC | 2,470,820 | IS2 (+) +5 bp | intergenic (‑177/+253) | yodE ← / ← yfdK | toxic protein YodE/putative tail fiber assembly protein YfdK |
RA | 2,474,885 | G→C | intergenic (+29/‑99) | yfdQ → / → yfdR | DUF2303 domain‑containing protein YfdQ/5'‑deoxynucleotidase |
RA | 2,577,987 | C→A | G131C (GGC→TGC) | maeB ← | malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+)) |
RA | 2,648,617 | T→C | D1224G (GAT→GGT) | yfhM ← | alpha2‑macroglobulin |
RA | 2,656,129 | C→T | G77D (GGT→GAT) | pepB ← | aminopeptidase B |
RA | 2,681,500 | G→A | P416S (CCT→TCT) | yphG ← | DUF5107 domain‑containing protein YphG |
JC JC | 2,698,402 | IS30 (+) +2 bp | coding (148‑149/636 nt) | pgpC ← | phosphatidylglycerophosphatase C |
RA | 2,731,034 | G→A | noncoding (124/1542 nt) | rrsG ← | 16S ribosomal RNA |
RA | 2,731,327 | C→T | intergenic (‑170/+273) | rrsG ← / ← clpB | 16S ribosomal RNA/chaperone protein ClpB |
RA | 2,751,849 | G→A | D19N (GAT→AAT) | recN → | DNA repair protein RecN |
RA | 2,780,701 | T→C | N676D (AAT→GAT) | ypjA ← | adhesin‑like autotransporter YpjA |
RA | 2,811,848 | G→A | S141N (AGC→AAC) | emrA → | multidrug efflux pump membrane fusion protein EmrA |
RA | 2,823,589 | G→A | R61C (CGT→TGT) | recA ← | DNA recombination/repair protein RecA |
RA | 2,866,783 | G→A | Q257* (CAG→TAG) | rpoS ← | RNA polymerase sigma factor RpoS |
RA | 2,867,923 | C→T | G277G (GGG→GGA) | nlpD ← | murein hydrolase activator NlpD |
RA | 2,873,316 | T→C | T8A (ACA→GCA) | ygbE ← | DUF3561 domain‑containing inner membrane protein YgbE |
RA | 2,878,434 | G→A | intergenic (+816/+135) | iap → / ← cas2 | alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2 |
RA | 2,891,085 | G→A | P272S (CCG→TCG) | cysJ ← | sulfite reductase, flavoprotein subunit |
RA | 2,903,727 | G→A | intergenic (+353/+1020) | ygcE → / ← queE | putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE |
RA | 2,913,761 | G→A | L414L (CTG→TTG) | rlmD ← | 23S rRNA m(5)U1939 methyltransferase |
RA | 2,930,364 | G→A | W263* (TGG→TGA) | sdaB → | L‑serine deaminase II |
RA | 2,937,789 | G→A | A108T (GCA→ACA) | fucK → | L‑fuculokinase |
RA | 3,017,470 | C→T | P471S (CCG→TCG) | ygfK → | putative oxidoreductase, Fe‑S subunit |
RA | 3,032,832 | C→T | R6C (CGC→TGC) | yqfG → | uncharacterized protein YqfG |
RA | 3,036,781 | G→A | F442F (TTC→TTT) | recJ ← | ssDNA‑specific exonuclease RecJ |
RA | 3,039,623 | G→A | H41Y (CAT→TAT) | xerD ← | site‑specific recombinase |
RA | 3,041,451 | G→A | A47T (GCA→ACA) | ygfZ → | folate‑binding protein |
RA | 3,065,497 | G→A | Q232Q (CAG→CAA) | scpC → | propionyl‑CoA:succinate CoA transferase |
RA | 3,182,389 | G→A | P16S (CCA→TCA) | ygiD ← | 4,5‑DOPA dioxygenase extradiol |
RA | 3,244,460 | G→C | H94Q (CAC→CAG) | uxaC ← | D‑glucoronate/D‑galacturonate isomerase |
JC JC | 3,252,952 | IS2 (–) +5 bp | coding (42‑46/357 nt) | yhaI → | putative inner membrane protein |
JC JC | 3,262,484 | IS5 (–) +4 bp | coding (1174‑1177/1209 nt) | tdcD ← | propionate kinase |
RA | 3,263,710 | C→T | L436L (CTG→CTA) | tdcC ← | threonine/serine:H(+) symporter |
JC JC | 3,361,732 | IS5 (–) +4 bp | coding (557‑560/765 nt) | gltF → | periplasmic protein GltF |
RA | 3,413,827 | G→A | intergenic (‑362/‑37) | envR ← / → acrE | DNA‑binding transcriptional repressor EnvR/multidrug efflux pump membrane fusion lipoprotein AcrE |
RA | 3,474,290 | T→C | K88R (AAA→AGA) | rpsL ← | 30S ribosomal subunit protein S12 |
RA | 3,486,205 | C→A | T29K (ACG→AAG) | crp → | DNA‑binding transcriptional dual regulator CRP |
RA | 3,555,759 | A→T | Q892L (CAA→CTA) | malT → | DNA‑binding transcriptional activator MalT |
RA | 3,560,455 | +G | intergenic (+589/+167) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
RA | 3,572,319 | T→G | E395A (GAA→GCA) | glgB ← | 1,4‑alpha‑glucan branching enzyme |
RA | 3,633,668 | T→A | intergenic (‑1078/‑1173) | yhiJ ← / → yhiM | DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM |
JC JC | 3,725,990 | IS5 (–) +4 bp | coding (104‑107/996 nt) | wecH → | O‑acetyltransferase WecH |
RA | 3,797,329 | C→T | L128L (CTA→TTA) | waaL → | O‑antigen ligase |
MC JC | 3,888,720 | Δ6 bp | intergenic (+211/‑5) | tnaC → / → tnaA | tnaAB operon leader peptide/tryptophanase |
RA | 3,957,805 | G→A | L6L (CTG→TTG) | ilvY ← | DNA‑binding transcriptional dual regulator IlvY |
RA | 3,971,703 | G→A | E148E (GAG→GAA) | wecC → | UDP‑N‑acetyl‑D‑mannosamine dehydrogenase |
RA | 3,977,463 | G→A | A396T (GCA→ACA) | wzxE → | lipid IIIECA flippase |
RA | 4,004,180 | G→A | intergenic (‑2/+50) | rarD ← / ← yigI | putative transporter RarD/putative thioesterase YigI |
RA | 4,018,586 | G→A | L418L (CTG→CTA) | rmuC → | putative recombination limiting protein RmuC |
RA | 4,149,535 | C→T | G244D (GGC→GAC) | eptC ← | phosphoethanolamine transferase EptC |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
RA | 4,309,712 | C→T | W423* (TGG→TGA) | alsA ← | D‑allose ABC transporter ATP binding subunit |
RA | 4,311,002 | 2 bp→GT | intergenic (‑22/+104) | alsA ← / ← alsB | D‑allose ABC transporter ATP binding subunit/D‑allose ABC transporter periplasmic binding protein |
RA | 4,392,517 | G→A | noncoding (46/76 nt) | glyX → | tRNA‑Gly |
RA | 4,515,556 | G→A | S374S (AGC→AGT) | fecA ← | ferric citrate outer membrane transporter |
RA | 4,521,151 | C→T | G290G (GGG→GGA) | yjhF ← | putative transporter YjhF |
JC JC | 4,577,765 | IS5 (–) +4 bp | coding (191‑194/1047 nt) | mcrC ← | Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit |
RA | 4,578,759 | G→A | I193I (ATC→ATT) I193I (ATC→ATT) |
mcrB ← mcrB ← |
5‑methylcytosine‑specific restriction enzyme subunit McrB 5‑methylcytosine‑specific restriction enzyme subunit McrB |
RA | 4,624,587 | G→A | S68L (TCA→TTA) | ytjB ← | putative inner membrane protein Smp |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 480924 | 481029 | 106 | 32 [0] | [0] 21 | tomB/acrB | Hha toxicity modulator TomB/multidrug efflux pump RND permease AcrB |
* | * | ÷ | NC_000913 | 1978495 | 1979270 | 776 | 29 [0] | [0] 22 | insB5–insA5 | insB5,insA5 |
* | * | ÷ | NC_000913 | 2558699 | 2560127 | 1429 | 35 [0] | [2] 3 | intZ | intZ |
* | * | ÷ | NC_000913 | 2560138 | 2560630 | 493 | 3 [2] | [2] 3 | [yffL] | [yffL] |
* | * | ÷ | NC_000913 | 2560808 | 2565488 | 4681 | 3 [1] | [2] 37 | [yffL]–[eutA] | [yffL],yffM,yffN,yffO,yffP,yffQ,yffR,yffS,[eutA] |
* | * | ÷ | NC_000913 | 3423907–3424231 | 3424424–3424238 | 8–518 | 3 [2] | [2] 3 | rrlD | 23S ribosomal RNA |
* | * | ÷ | NC_000913 | 4215400 | 4216928 | 1529 | 30 [2] | [2] 3 | [aceB] | [aceB] |
* | * | ÷ | NC_000913 | 4217634 | 4218626 | 993 | 3 [2] | [2] 3 | [aceA]–[aceK] | [aceA],[aceK] |
* | * | ÷ | NC_000913 | 4218739 | 4221609 | 2871 | 3 [2] | [2] 3 | [aceK]–[arpA] | [aceK],aceK‑int,[arpA] |
* | * | ÷ | NC_000913 | 4222422 | 4223855 | 1434 | 3 [2] | [2] 3 | [arpA]–[metH] | [arpA],iclR,[metH] |
* | * | ÷ | NC_000913 | 4224643 | 4225518 | 876 | 3 [2] | [2] 3 | metH | cobalamin‑dependent methionine synthase |
* | * | ÷ | NC_000913 | 4225730 | 4225951 | 222 | 3 [2] | [2] 3 | metH | cobalamin‑dependent methionine synthase |
* | * | ÷ | NC_000913 | 4226302 | 4226579 | 278 | 3 [2] | [2] 3 | metH | cobalamin‑dependent methionine synthase |
* | * | ÷ | NC_000913 | 4226758 | 4227755 | 998 | 3 [2] | [2] 3 | [metH]–[yjbB] | [metH],[yjbB] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 381260 = | NA (NA) | 21 (0.520) | 21/496 | 0.7 | 100% | noncoding (1/1331 nt) | IS2 | repeat region |
? | NC_000913 | 481030 = | 0 (0.000) | intergenic (‑322/+224) | tomB/acrB | Hha toxicity modulator TomB/multidrug efflux pump RND permease AcrB | |||||
* | ? | NC_000913 | = 382590 | NA (NA) | 32 (0.790) | 28/496 | 0.4 | 100% | noncoding (1331/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 480923 | 0 (0.000) | intergenic (‑215/+331) | tomB/acrB | Hha toxicity modulator TomB/multidrug efflux pump RND permease AcrB | |||||
* | ? | NC_000913 | = 566774 | 49 (1.210) | 17 (0.420) | 9/492 | 1.6 | 26.7% | pseudogene (91/93 nt) | renD | protein RenD |
? | NC_000913 | 568036 = | 45 (1.120) | pseudogene (2/213 nt) | renD | protein RenD | |||||
* | ? | NC_000913 | 687851 = | NA (NA) | 30 (0.740) | 30/496 | 0.3 | 52.6% | noncoding (1195/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 821414 | 27 (0.670) | coding (622/714 nt) | ybhM | Bax1‑I family protein YbhM | |||||
* | ? | NC_000913 | 1207790 = | 3 (0.070) | 8 (0.210) | 8/464 | 1.7 | 80.8% | coding (290/630 nt) | ycfK | protein StfP |
? | NC_000913 | 1209619 = | 1 (0.030) | intergenic (‑500/‑66) | tfaE/pinE | putative tail fiber assembly protein TfaE/site‑specific DNA recombinase | |||||
* | ? | NC_000913 | = 1207805 | 2 (0.050) | 13 (0.340) | 13/464 | 1.1 | 90.1% | coding (305/630 nt) | ycfK | protein StfP |
? | NC_000913 | = 1209602 | 1 (0.030) | intergenic (‑483/‑83) | tfaE/pinE | putative tail fiber assembly protein TfaE/site‑specific DNA recombinase | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 8 (0.200) | 8/488 | 1.8 | 100% | intergenic (+253/‑69) | ychE/insH21 | MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+151/‑484) | insH21/oppA | IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | = 1397238 | NA (NA) | 29 (0.710) | 26/496 | 0.5 | 100% | noncoding (1195/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 1978494 | 0 (0.000) | intergenic (‑297/+24) | flhD/insB5 | DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB | |||||
* | ? | NC_000913 | 1979271 = | 0 (0.000) | 22 (0.540) | 20/496 | 0.7 | 100% | intergenic (‑56/‑482) | insA5/uspC | IS1 family protein InsA/universal stress protein C |
? | NC_000913 | = 2102947 | NA (NA) | pseudogene (443/446 nt) | wbbL | interrupted rhamnosyltransferase WbbL | |||||
* | ? | NC_000913 | 2288919 = | NA (NA) | 28 (0.690) | 28/496 | 0.4 | 93.3% | noncoding (1195/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 4215399 | 2 (0.050) | intergenic (+190/‑79) | metA/aceB | homoserine O‑succinyltransferase/malate synthase A | |||||
* | ? | NC_000913 | = 2290113 | NA (NA) | 28 (0.690) | 26/496 | 0.5 | 56.0% | noncoding (1/1195 nt) | IS5 | repeat region |
? | NC_000913 | 3746186 = | 22 (0.540) | coding (93/987 nt) | yiaO | 2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ periplasmic binding protein | |||||
* | ? | NC_000913 | = 2558698 | 0 (0.000) | 35 (0.890) | 34/480 | 0.2 | 100% | intergenic (‑19/‑160) | eutB/intZ | ethanolamine ammonia‑lyase subunit alpha/putative phage integrase IntZ |
? | NC_000913 | 2565489 = | 0 (0.000) | coding (1396/1404 nt) | eutA | ethanolamine ammonia‑lyase reactivase EutA | |||||
* | ? | NC_000913 | 2765412 = | 11 (0.270) | 18 (0.540) | 16/408 | 0.7 | 76.5% | intergenic (‑259/+101) | abpA/yfjM | anti‑bacteriophage protein/protein YfjM |
? | NC_000913 | = 2765419 | 2 (0.060) | intergenic (‑266/+94) | abpA/yfjM | anti‑bacteriophage protein/protein YfjM | |||||
* | ? | NC_000913 | = 2947467 | 15 (0.370) | 5 (0.190) | 5/330 | 1.7 | 38.5% | intergenic (+4/‑30) | metZ/metW | tRNA‑Met/tRNA‑Met |
? | NC_000913 | 2947688 = | 6 (0.220) | intergenic (+5/+69) | metV/amiC | tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C | |||||
* | ? | NC_000913 | 3042321 = | 32 (0.790) | 34 (0.950) | 32/438 | 0.2 | 62.3% | intergenic (+28/+168) | ygfZ/yqfA | folate‑binding protein/hemolysin‑III family protein YqfA |
? | NC_000913 | = 3042439 | 13 (0.360) | intergenic (+146/+50) | ygfZ/yqfA | folate‑binding protein/hemolysin‑III family protein YqfA | |||||
* | ? | NC_000913 | 3186096 = | NA (NA) | 23 (0.570) | 23/494 | 0.6 | 43.4% | noncoding (1/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 4366042 | 30 (0.740) | coding (732/1302 nt) | dcuA | C4‑dicarboxylate transporter DcuA | |||||
* | ? | NC_000913 | = 3653230 | NA (NA) | 52 (1.280) | 46/496 | 0.1 | 91.2% | noncoding (1/1195 nt) | IS5 | repeat region |
? | NC_000913 | 4228848 = | 5 (0.120) | coding (1118/1632 nt) | yjbB | putative inorganic phosphate export protein YjbB | |||||
* | ? | NC_000913 | 4366038 = | 29 (0.710) | 23 (0.570) | 22/496 | 0.6 | 44.2% | coding (736/1302 nt) | dcuA | C4‑dicarboxylate transporter DcuA |
? | NC_000913 | = 4499511 | NA (NA) | noncoding (1331/1331 nt) | IS2 | repeat region |