Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	447,538	A→G	F56F (TTT→TTC)	cyoE ←	heme O synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	447,538	0	A	G	97.1%	113.4 / ‑4.8	35	F56F (TTT→TTC)	cyoE	heme O synthase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (18/16);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

CGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCACATAATCCAGATGGAGCCTACAACCAGGATAGCG  >  NC_000913/447298‑447783                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      cGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATAACCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAAc                                                                                                                                                                                                                                             >  1:269566/1‑251 (MQ=255)         aGACAGAGTGGCGTTTCATGTACAGGCGATAAACGCCGACATAAACCACAAAGCCCATCCCCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCGTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGcc                                                                                                                                                                                                                                     <  1:405853/251‑1 (MQ=255)                  ggCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGGTGTTGAACACACAACCCGACGCCACAACCAGt                                                                                                                                                                                                                            <  1:174909/251‑1 (MQ=255)                     gTTTCATGTACAGGCTATAAACGCCGGCATAAACCGCTAATCCCATCTCCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCGCAGCAGCATAAAGCCAGCAATACCCATCAACGTGGCGTACACTATCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCTCCCTATGCTTCGTCCTTTCCATCTGTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGac                                                                                                                                                                                                                         <  2:413272/251‑1 (MQ=255)                              aCAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGAACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGCCGCCACAACCCATGACACCCCAAcc                                                                                                                                                                                                                >  2:334937/1‑251 (MQ=255)                                aGGCTAAAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCCTAAAGCCACCAATACCCAGCACCGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGCTGTAGTTGTTGAACCCACAACCCGACACCACAACCAGTGACACCCAAAcc                                                                                                                                                                                                                >  1:271427/1‑249 (MQ=255)                                           gCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGCGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATa                                                                                                                                                                                                    >  1:435128/1‑250 (MQ=255)                                           gCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATaa                                                                                                                                                                                                   <  1:216165/251‑1 (MQ=255)                                            ccGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATaaa                                                                                                                                                                                                  <  1:212910/251‑1 (MQ=255)                                                     aCCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGAt                                                                                                                                                                                          >  1:42708/1‑250 (MQ=255)                                                        acaAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATc                                                                                                                                                                                      <  1:354154/251‑1 (MQ=255)                                                         caAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTAGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCCGTGACACACCAACCAGCGTGTAGATAAACAGGGGATAAta                                                                                                                                                                                      >  1:340367/1‑249 (MQ=255)                                                                            ccagcAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTGTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGAGGTAGTTTTTGAACACACAGCCCGACGCGACAGCCTGTGACACCCCATGCAGCGTGTAGATAAACAGTGGATAATCAATGCTGCCCTTTTAGGCca                                                                                                                                                                  <  2:275721/251‑1 (MQ=255)                                                                                                        cacaGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAg                                                                                                                                      <  1:13804/251‑1 (MQ=255)                                                                                                              agcaTAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGcc                                                                                                                                <  1:302410/251‑1 (MQ=255)                                                                                                                   aaaGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAga                                                                                                                           <  1:126834/251‑1 (MQ=255)                                                                                                                    aaGCCAGCAATCCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAgat                                                                                                                          <  2:78073/251‑1 (MQ=255)                                                                                                                    aaGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAgat                                                                                                                          <  1:84500/251‑1 (MQ=255)                                                                                                                                 ccAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTc                                                                                                             >  1:348561/1‑251 (MQ=255)                                                                                                                                         gTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCAATCTTTCTGTCGATATCCCTGTCGATGTAGTTGCTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAACCAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTg                                                                                                     >  1:197836/1‑251 (MQ=255)                                                                                                                                                 cacCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTGTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTGGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTAtt                                                                                             <  2:66480/251‑1 (MQ=255)                                                                                                                                                  acCAGCGAGACAGCAGGAGAGCTCAGGCCTTTCACCAGCACCCGATTCTTCGCCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGTAATCCCCCAATCACCGAGCTCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTg                                                                                            >  2:75673/1‑251 (MQ=255)                                                                                                                                                    cAGCGAGACAGCAGGCGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTAGTTACTTGCAGGTATTGCt                                                                                          >  2:276008/1‑251 (MQ=255)                                                                                                                                                            cagcagGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCCTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGAGACACCCCAACCAGCGCGTAGATCAACAGGGGATAATCAATGCTGCCCTTTGCGGCAAGCAGGCATCCCCCAATCACCGAGATCAGATTGCCAAAGATGATGCCTGGTTTCCTTACATGCAGGTATTGCTTAAAcatc                                                                                  >  1:438306/1‑251 (MQ=255)                                                                                                                                                                agGAGAGAATAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCTCAACCAGTGACACCCCAACCAGCGTGTAGATAATCAGGGGATAATCAATGCTGCCCTTGGAGGCCAGCAGGAATCCCCCAAGCACCGAGATCAGGTTTCCAAAGATGATGCCTGGTTGCGTGGCTTGCAGGTATTGCTTAAACATCATaa                                                                              <  2:274747/251‑1 (MQ=255)                                                                                                                                                                 ggAGAGATCAGTCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGGCAGCAGGAATCCCCCTATCACCGAGATCAGGTTGCCAAAGATGAGGCCTGGTGTCGTTACTTGCAGGTATTGCTTAAACATCATAAc                                                                             <  1:165661/251‑1 (MQ=255)                                                                                                                                                                     agaTCAGGCCTTGCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTGGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCGGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAAccgcc                                                                         <  2:179750/251‑1 (MQ=255)                                                                                                                                                                      gaTCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGTAATCCCCCAATCACCGAGATCAGGTTGCCACAAATGATGCCTGGTTTCGTTACTTGCAGGAATTGCTTAAACATCAAAAccgccg                                                                        >  2:372110/1‑251 (MQ=255)                                                                                                                                                                              cTTTCAACAGCACGCGATTCTTCGTCCTTTACATCTTTCTGTCGATATCCCTGTCGCTGCAGTTGTTGAACACAAAACACGACGCTACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGCATACCCCAATCAACGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTCCTTGCATGTATTGCTTAAACATCATAACCGCCGctctccgt                                                                >  2:288243/1‑247 (MQ=255)                                                                                                                                                                                                  ttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATATAGCTGTTGAACACACAACACGACGCCACCACCAGTGACACCCAAACAACCGAGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGACAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTg                                            >  1:402025/1‑251 (MQ=255)                                                                                                                                                                                                  ttcGTCCTTTCCACCTTACAGTCGATCTCCCTGTCAATGTAGTTGCTGATCACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCCAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATCACCGCCGCTCTTCGTGCATCATCATGTTGTAGTTg                                            >  2:58727/1‑251 (MQ=255)                                                                                                                                                                                                    cgtccttccattctttctggtgaactCCCGGTCGAGGTATTTGTTAAACACACACCCCGCCGCCACAACCAGGCACCCCCCAACCAGAGGGAAAAAAAACGGGGGAAAATCAGTGCTGCCTTTGGGGGCAAGAAGGACCCCCCCAACCACCGAGATCGGTTTGCAAAAAAGGATGCCTGTTTGCTTTCCTGGCAGGTATTGCGAAAACAAAAAAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAg                                          <  1:211295/226‑1 (MQ=255)                                                                                                                                                                                                         tttCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTcc                                     >  1:325840/1‑251 (MQ=255)                                                                                                                                                                                                                         gATATCCCTGTCGATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGAGCATCATCATGTTGTAGTTGAGGTTCCACATAATCCAGATgg                      >  1:34125/1‑250 (MQ=255)                                                                                                                                                                                                                                     gATGTAGTTGTTGAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCACATAATCCAGATGGAGCCTACAAcc           >  1:56857/1‑249 (MQ=255)                                                                                                                                                                                                                                            ttgttgAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCACATAATCCAGATGGAGCCTACAACCAGGATAGCg  >  1:320036/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      CGTGCCGTAGACAGAGTGGCGTTTCATGTACAGGCTATAAACGCCGACATAAACCACAAAGCCCATCACCCCCAGCCAGCAGGCCAGCGGATTCGCGCCAAACCACAGCAGCATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCACATAATCCAGATGGAGCCTACAACCAGGATAGCG  >  NC_000913/447298‑447783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑