Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,309,712	C→T	W423* (TGG→TGA)	alsA ←	D‑allose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,309,712	0	C	T	92.3%	107.0 / NA	39	W423* (TGG→TGA)	alsA	D‑allose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (14/22);  minor base G (0/3);  total (14/25)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

ATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGCCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATG  >  NC_000913/4309476‑4309955                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    aTCTCCTTTTCGATCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGCTCAGATGACACCATCAGGGTGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGAGCCAACGTCGCTGCCGCGGGTAGGTTCATCGAAAATAATAAATTGCGGGCAACAGCACAGTCATTTGTAGATc                                                                                                                                                                                                                                         >  2:69827/1‑249 (MQ=255)     cctcTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAgg                                                                                                                                                                                                                                      <  2:11251/248‑1 (MQ=255)             cTCATGTCATCGCGATTCGTCCAGATTTGCGTCAGTCGTCCTTCGCCGAACACGGCGATGCGGTCGCAGAAGGTGATAATTTCAGATCGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGCTCAGGACTTTctcctg                                                                                                                                                                                                                           >  2:292572/1‑251 (MQ=255)              tCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGGCACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTctgctg                                                                                                                                                                                                                           >  1:288061/1‑250 (MQ=255)                       cgcgATTCGTCAGGATTTGCGTCAGGCGTCCGTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCg                                                                                                                                                                                                                 <  1:173937/251‑1 (MQ=255)                        gcgAGTCGTCAGGATTTGCGTCAGGCGTCCTTCGCAGAACCCGGCGATGCGGGCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGGCATTTGGAGATCAGGACTTTctgctgtgtttccccgg                                                                                                                                                                                                                <  2:142892/251‑12 (MQ=255)                                       ttGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCAGTGata                                                                                                                                                                                                  <  1:117925/250‑1 (MQ=255)                                             cAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTgg                                                                                                                                                                                            <  1:292572/250‑1 (MQ=255)                                                       tCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTGCGCGCCAGCTTCTATGCCGCTGGTAGGTTCATCGAAAATAATCACGTGCGGGCAACAGCACAGTGATTTGGAGATGAGGACTTTCGGCTGAGGTCCTCCGGAGAGTTCGGTGATATTCTGGTTTACCGAAt                                                                                                                                                                                  <  1:181444/250‑1 (MQ=255)                                                        cGCAGAACACGGCGATGCTGGGGACGACGTTGATAATTTCAGGTAGTTCAGATGACTCCATCATGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAGTTTCGGCTTTCGCGCCATCGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCTGGCTAGAGGACAGGCATTTGTAGATCAGGTCTTTCTGCTGATTGCCCGCGGATAGTTCGGTGATGTTCTGGtgtaactaatga                                                                                                                                                                                <  2:178498/251‑12 (MQ=255)                                                         gCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGAc                                                                                                                                                                               <  1:354420/251‑1 (MQ=255)                                                               cacGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCa                                                                                                                                                                         <  1:148992/251‑1 (MQ=255)                                                               cacGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTGCGGTGATATTCTGGTTTACCGAATGACATTTCa                                                                                                                                                                         <  1:58729/251‑1 (MQ=255)                                                                 cGGCGATGCGGACGCACACGGTGATAATTTCAGGCCGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGCGAGTTCGGCGATATTCTGGTTTCCCGAATTACATTTCAgc                                                                                                                                                                       >  2:270920/1‑251 (MQ=255)                                                                        gCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCcagca                                                                                                                                                                <  1:69777/251‑1 (MQ=255)                                                                        gCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCcagca                                                                                                                                                                <  1:304196/251‑1 (MQ=255)                                                                          tccgatcgACGGTGATAATTTCAGGTAGTTCAGATGATCCCATCAGGAGGACTTTTCCGTCGTTCGCCATTTGGCGCTTCACTTTGTAAAGGTCGGCTTTCGCGCCAACGTCGATGCCGCGGGGAGGTTCATCGAAAATAAGCACTTCCGGGGAACATCACAGTCTGTTGGAGATCAGGACGTTCTGCTGATTTCCCCCGGAGAGTTCTGTGAGAGTCTGGTTTACCGAATGACATTTCAGCGCcagcag                                                                                                                                                               <  1:195527/243‑1 (MQ=255)                                                                           gTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGtt                                                                                                                                                             <  1:371090/251‑1 (MQ=255)                                                                                aGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGt                                                                                                                                                        >  1:179081/1‑251 (MQ=255)                                                                                 gACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGGCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGtt                                                                                                                                                       <  1:207447/251‑1 (MQ=255)                                                                                                aGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTAc                                                                                                                                        <  1:429487/251‑1 (MQ=255)                                                                                                       gtaGATGACACCATCAGGAAGAGTGTGCCGGCTTCCGCCAGTTGGCGCATCACTTTGTAAAGTTCGGCTATCGCGCCAACGGCGATGCCGAGGGTAGGTTCATAGAAAATAAGCACTTCCGGGCAACAGCACAGTCATTTGGAGAGCAGGACTTTCTGCGGGTTTCCAACGGAGAGTTTGGTGAGATGCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTGCGCGTGGATTGTCAGCGGTACTTTGCt                                                                                                                                  <  2:30963/248‑1 (MQ=255)                                                                                                                                                ttgtcgcATCTCTTTTTAAATTTCTGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAgccg                                                                                         <  2:7491/246‑1 (MQ=255)                                                                                                                                                  ggCGTATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAgccgac                                                                                       <  2:83168/250‑3 (MQ=255)                                                                                                                                                    cgcATCTCTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTc                                                                                    <  2:288061/251‑1 (MQ=255)                                                                                                                                                       aTCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCttt                                                                                 >  1:27394/1‑251 (MQ=255)                                                                                                                                                                 aaaTTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCTCTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCTGTGATATTCTGGTTTACCGAATGACATTTCATCGCCAGCAGTTCGCGTTGATGTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCTCCTTTATAGCCGCCGTCTTTCAGACTGCg                                                                        <  1:311233/250‑1 (MQ=255)                                                                                                                                                                 aaaTTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCgg                                                                       >  1:325479/1‑251 (MQ=255)                                                                                                                                                                       cGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGAt                                                                  <  1:171318/250‑1 (MQ=255)                                                                                                                                                                        ggCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGCTCg                                                                >  1:66831/1‑251 (MQ=255)                                                                                                                                                                                   cAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGa                                                     <  1:192183/251‑1 (MQ=255)                                                                                                                                                                                             gCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGaa                                            <  1:316735/250‑1 (MQ=255)                                                                                                                                                                                                      aggatCATCGAAAATAATCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTgggg                                   >  1:445362/5‑250 (MQ=255)                                                                                                                                                                                                               gAAAATACTCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGt                         >  1:341196/1‑251 (MQ=255)                                                                                                                                                                                                                  aataatCACTTCCGGGCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTAt                      <  1:270920/251‑1 (MQ=255)                                                                                                                                                                                                                     aatCACTTCCGGGCAACAGCACAGTCATTTGGCGATCAGGACTTCCTGCTGATTACCCCCGGAGAGATAGGTGATATTCTGGTTTACCGAATGACATTTCCGCGCCCGCCGTTCGCGTTGATCTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACACGCCCCTCGCGCCTTTTTAGCCGCCGTCTTTCATACTGCGGCTAATCGCCCTGTTCTGAGCGATGGAAACGATGGGGAAAAAACAGGTATccc                   >  1:436921/1‑251 (MQ=255)                                                                                                                                                                                                                               gggCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTc         >  1:49311/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCAACAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAACGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTc         >  2:78958/1‑249 (MQ=255)                                                                                                                                                                                                                                   aaCAGCACAGTCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTCCCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTg     >  1:275015/1‑251 (MQ=255)                                                                                                                                                                                                                                      aacacagtCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGTAGAGTTCGGTTATAGGCGGGGTTACCGAAGGACATTTCAGCGCCATCAGGTCGCGTGGAATTGCAGCCGTACGTTGCTCGTCAACTGCATGAAACAAGCCCATTGCGCCGTTAGAGACGCCGTCTTTCAGACTGCGGCTGAGCGCCAGGGTCTGCTCGATGGAAAAGGTGGGGAAAAAACCTTTATCCCTGCGGCTTGCAGTGagg  <  2:359993/249‑3 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    ATCTCCTCTTCGCTCATGTCATCGCGATTCGTCAGGATTTGCGTCAGTCGTCCTTCGCAGAACACGGCGATGCGGTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCATCACTTTGTAAATTTCGGCTTTCGCGCCAACGTCGATGCCGCGGGTAGGTTCATCGAAAATAATCACTTCCGGGCAACAGCACAGCCATTTGGAGATCAGGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATG  >  NC_000913/4309476‑4309955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑