Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,268,985	G→A	Q44Q (CAG→CAA)	lpxT →	lipid A‑core phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,268,985	0	G	A	100.0%	72.3 / NA	23	Q44Q (CAG→CAA)	lpxT	lipid A‑core phosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

GAAGCTGACTGGAATGCCTTGCAGAGCGCGCTGTTGAAGCTTCGTTTAGCGACTACCGCGTAAGGTTGCCTGCGTTTTTCAGTAAGATAATTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCTGGGTCAGGCATTAATTCCTGTAAAACGGGCCAGCCCAACATTGACTT  >  NC_000913/2268753‑2269223                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               gAAGCTGACTGGAATGCCTTGCAGAGCGCGCTGTTGAAGCTTCGTTTAGCGACTACCGCGTAAGGTTGCCTGCGTTTTTCAGTAAGATAATTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAAATGGTCGAAAGTa                                                                                                                                                                                                                                >  1:376795/1‑249 (MQ=255)                gCCTTGCAGAGCGCGCTGTTGAAGCTTCGTTTAGCGACTACCGCGTAAGGTTGCCTGCGTTTTTCAGTAAGATAATTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCt                                                                                                                                                                                                                <  1:333126/250‑1 (MQ=255)                             cgcTGTTGAAGCTTCGTTTAGCGACTACCGCGTAAGGTTGCCTGCGTTTTTCAGTAAGATAATTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACca                                                                                                                                                                                                  <  1:36839/251‑1 (MQ=255)                              gcTGTTGAAGCTTCGTTTAGCGACTACCGCGTAAGGTTGCCTGCGTTTTTCAGTAAGATAATTCGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACca                                                                                                                                                                                                  >  2:430599/1‑250 (MQ=255)                              gcTGTTGAAGCTTCGTTTAGCGACTACCGCGTAAGGTTGCCTGCGTTTTTCAGTAAGATAATTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACcaa                                                                                                                                                                                                 <  1:31278/251‑1 (MQ=255)                                                aGCGACTACCGCGTAAGGTTGCCTGCGTTTTTCAGTAAGATAATTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACg                                                                                                                                                                                <  1:250984/250‑1 (MQ=255)                                                               aGGTTGCCTGCGTTTTTCAGTAAGATAAGTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGc                                                                                                                                                                <  2:123616/251‑1 (MQ=255)                                                                        gCGTTTTTCAGTAAGATAATTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTa                                                                                                                                                        <  1:315267/250‑1 (MQ=255)                                                                                                                     ccGCAAATAGCGTTGATGAATATTGTCGACCTCGCGCTGTTTCTTTCCTGGTATATCCAGGTTAATCCTGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTAGTTGAATTGACCAAAAAACGCGACTTCGACGGTTGTTACCTGCTGGCGCTGGGTATGTTGATGCTGAGTTTCTTGCTGAAAGAAAACGCACCTTGCAga                                                                                                            >  2:404299/1‑249 (MQ=255)                                                                                                                          aaTAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTa                                                                                                     <  1:347668/251‑1 (MQ=255)                                                                                                                           aTAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTATTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGCTATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATAGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGacgacgaat                                                                                                    >  1:254380/1‑248 (MQ=255)                                                                                                                               tgttgtTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTg                                                                                                >  1:275660/1‑251 (MQ=255)                                                                                                                                         tatTGTCGGCCTCGCGCTGTTTCTTTCATGGGATATCCCCGTTAATCATGGATTATGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCAATGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGGTTATtggtg                                                                                      >  2:66015/1‑250 (MQ=255)                                                                                                                                              tCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctggtc                                                                                 >  1:348123/1‑251 (MQ=255)                                                                                                                                                ggCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATGCTGGTTGCCGATTGAGGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGTTTGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTTGCGTAAAGAAAACGCCCCGGGCAGACGACGTATCGTGATTATTGGTCTGGTCAt                                                                               <  1:395198/251‑1 (MQ=255)                                                                                                                                                      gcgcTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGGTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGACAGCAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCCTGCTAtt                                                                         >  1:254538/1‑251 (MQ=255)                                                                                                                                                        ggtGTTTATTTCCGGGTATATTCCCGTTAATCATGGATTCTGGTTGCCGACTGATGCGTGTTTTTTTTATTTCTTTAATCAAAAACTGGTCGTAGGTAAGGTCTCTTTGTGGCTTGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCCGACGACGTATCGTGTTTATTGGTCTGGTCATGCTATTaa                                                                       <  2:316375/249‑1 (MQ=255)                                                                                                                                                                 ttcCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAg                                                                 <  1:336226/248‑1 (MQ=255)                                                                                                                                                                 ttcCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTg                                                               <  1:239812/250‑1 (MQ=255)                                                                                                                                                                       gTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGCTGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTa                                                         >  1:21532/1‑250 (MQ=255)                                                                                                                                                                                         tggcttCTGGTTGCCGCTTAATGCGGATATTTTTTATTTCTTTAATCAAAAACTGGCCGAATGTAAGGCCTTTTTGTGGCTGGTTGCCTTGACCAACAATCCCACCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGCTGCTGCGTTTCTGGCGGAACGACAGCGCCCCTGGCCGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGACTTAAACCAGCTGGGTCAGGCAt                                      >  2:405443/5‑251 (MQ=255)                                                                                                                                                                                                               gCGGATATTTTTTATTTCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCTTGGTCAGGCATTAATTCCTGTAAAACGGGCCAg                <  1:197402/251‑1 (MQ=255)                                                                                                                                                                                                                             tttCTTTAATCAAAAACTGGTCGAAAGTAAGGCCTTTTTGTGGTTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGCTGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCTGGGTCAGGCATTAATTCCTGTAAAACGGGCCAGCCCAACATTGACtt  >  1:271025/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               GAAGCTGACTGGAATGCCTTGCAGAGCGCGCTGTTGAAGCTTCGTTTAGCGACTACCGCGTAAGGTTGCCTGCGTTTTTCAGTAAGATAATTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCTGGGTCAGGCATTAATTCCTGTAAAACGGGCCAGCCCAACATTGACTT  >  NC_000913/2268753‑2269223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑