Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	928,622	G→A	L192L (CTG→TTG)	cydC ←	glutathione/L‑cysteine ABC exporter subunit CydC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	928,622	0	G	A	97.0%	96.9 / NA	33	L192L (CTG→TTG)	cydC	glutathione/L‑cysteine ABC exporter subunit CydC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (20/12);  minor base C (1/0);  total (21/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAGATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCC  >  NC_000913/928382‑928868                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       cgccAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGGCCGCGAAGATGAGTCAAATTTTGCCCg                                                                                                                                                                                                                                              <  2:166025/251‑1 (MQ=255)      aCGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCt                                                                                                                                                                                                                                         <  1:439977/251‑1 (MQ=255)        gccgcaataCGCCATCCGCATCATCAGGATCACCGCGAACGCGCCAATGAGCATCATTATCGCTTGCGACAAGGTGGTCGGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCGCTAGTTGCGTGCGATAACGATGGCTGGCACCAAAAATGGGCAGCTCAGCTTGCCCTGGCAGCCAGGCCGGCAGTTGTTGGCAATACTGTCCGCGAAGATGATTCAAATTTTGCCCGGTGCttt                                                                                                                                                                                                                                       <  2:175893/243‑1 (MQ=255)         ccgcaaggctccATCCACAGCATCGAGAACACCTCTTACGCGCCTAGGGGCAGCATTAGCGCTTGCGATAAGGCGGTCAGTTCATATTGACGGCGTTGCGCTGCCAGGCATTGAATTACTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCGTTGCAGTCAGGCAGTCAGTTTTTGGCGATACTGTCCTCGAAGATGATTCAAATTTTGcactctgctttt                                                                                                                                                                                                                                      <  2:392417/241‑12 (MQ=255)              aGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCg                                                                                                                                                                                                                                 >  2:322939/1‑251 (MQ=255)                   ccATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGAGTGTCGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACTATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGGTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCTCACGa                                                                                                                                                                                                                            <  2:191943/251‑1 (MQ=255)                      tCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGCATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATa                                                                                                                                                                                                                          >  1:378485/1‑250 (MQ=255)                                  ggATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGGGCTTTTTCCCGCACGATAAAACAGCGGTGGc                                                                                                                                                                                                             >  2:179220/1‑251 (MQ=255)                                    aTCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATCTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCAGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCAt                                                                                                                                                                                                           >  1:161168/1‑251 (MQ=255)                                     tCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAGGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATc                                                                                                                                                                                                          >  1:349968/1‑251 (MQ=255)                                                 cgcCAATGAGCATGATTATCGATTGCGACAATGCGGTCAGGTCAGATTGACGGCTTTGCGCTTCCGTCCATTGAATTTCTGTATGCTCTAGTGGCGTGCGATAACGATCGCTGGCGCCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGACAGGCCGTCAGTAGTTGGGGATACGTTCCGCGAAGTGGTGTCAAATTGTGTCCGTTGCTTTTTCCCGCGCGATAAAACAGCTGTGGCATCAGGAAAGGCGTc                                                                                                                                                                                              <  2:279351/251‑1 (MQ=255)                                                        tagaagcaTTATCGCTTGCGACAATGCGGTCAGATCAGATTGACGGCGTTGCGCTTCCAGCCATTGGATTTCTGTATTCTCTAGTTGCGTGCGTTAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCCGTTGGTGGCGCTACTGTCCGCGAAGCTGAGTCAAATTTTGCCCGGTGCTTTTTTCCGCACGCTAAACCAGCGGTGGAATCAGGAAAAGCGTccttaac                                                                                                                                                                                        >  2:321214/5‑244 (MQ=255)                                                                ttATCGCTTGCGACAATGCGGACAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTTAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTACGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGCTAAAACAGCGGTTGCATCAGGAAAAGCTTCAGTAACATACTgccg                                                                                                                                                                               >  2:454354/1‑251 (MQ=255)                                                                                   ggTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAggg                                                                                                                                                            >  1:318138/1‑251 (MQ=255)                                                                                      cAGTTCAGATGGCCGGCGTTGCGCTGCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCCCCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCCAGGGTg                                                                                                                                                          <  2:103213/250‑1 (MQ=255)                                                                                       cgTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATCTCTGTATTCTCGAGTTGCGTGCGATAACTATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATTCTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTTCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCATGAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGaa                                                                                                                                                        <  2:161168/250‑1 (MQ=255)                                                                                                   cGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACt                                                                                                                                            <  1:344943/251‑1 (MQ=255)                                                                                                       gTTGCGCTTCCAGCCATTGAATTTCTGTATACTCTAGTTGCGTGCGCTAACGATCGCTGGCACCAACAATGGTCAGCTCAGCTTGCCCTTGCAGCCCGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGCGAAATCAAGGAAACTTAAc                                                                                                                                        >  1:269008/1‑251 (MQ=255)                                                                                                                ccAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTc                                                                                                                               <  1:305133/251‑1 (MQ=255)                                                                                                                   gCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTcacc                                                                                                                            >  1:63633/1‑251 (MQ=255)                                                                                                                                  tATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATcaccac                                                                                                              >  2:217298/1‑250 (MQ=255)                                                                                                                                           gTTGCGTGCGATAACGAGCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATGTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGccc                                                                                                    <  1:160797/251‑1 (MQ=255)                                                                                                                                             tgcgtgcgATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCccac                                                                                                  >  1:353869/1‑251 (MQ=255)                                                                                                                                                 tgcgATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAgc                                                                                              >  1:341030/1‑251 (MQ=255)                                                                                                                                                     aTAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAgcggcg                                                                                          >  1:332251/1‑251 (MQ=255)                                                                                                                                                                             ggTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAgatgat                                                                  >  1:77873/1‑251 (MQ=255)                                                                                                                                                                              gTCAGCTCAGCTTGCCCTTGCAGACAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCACATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGCTTGTCACCACCATAATCACCACAACAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATc                                                                 >  2:384499/1‑251 (MQ=255)                                                                                                                                                                               tcagctcagcTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGACCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCACGGAAACTTAACCCGATTGTCACCACCATAATCACCCCAAAAGCGCCCACCAGAGCCGAGATAACGCGCAAGCAAAGATGATCg                                                                >  2:335795/1‑251 (MQ=255)                                                                                                                                                                                              cTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGCGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGAGCAGGTAAAGATGATCGAGCGTATCAACATcc                                                 >  1:391642/1‑251 (MQ=255)                                                                                                                                                                                                tGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCg                                                <  1:308877/250‑1 (MQ=255)                                                                                                                                                                                                tGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGc                                               >  1:268098/1‑251 (MQ=255)                                                                                                                                                                                                                              tGTCCGCGAAGATGAGTCAAATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACg                 >  1:102955/1‑251 (MQ=255)                                                                                                                                                                                                                                             gTCAAATTTTGCACGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGAAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTcc  >  2:427728/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       CGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGCTTCCAGCCATTGAATTTCTGTATTCTCTAGTTGCGTGCGATAACGATCGCTGGCACCAAAAATGGTCAGCTCAGCTTGCCCTTGCAGCCAGGCCGTCAGTTGTTGGCGATACTGTCCGCGAAGATGAGTCAGATTTTGCCCGGTGCTTTTTCCCGCACGATAAAACAGCGGTGGCATCAGGAAAAGCGTCAGTAACATAATGCCGCCCAGCGTAAAGGCGAGGGTGAAATCAAGGAAACTTAACCCGATTGTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCC  >  NC_000913/928382‑928868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑