Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,061,989	T→C	intergenic (‑56/‑271)	nac ← / → asnV	DNA‑binding transcriptional dual regulator Nac/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,061,989	0	T	C	100.0%	64.1 / NA	22	intergenic (‑56/‑271)	nac/asnV	DNA‑binding transcriptional dual regulator Nac/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/14);  total (8/14)

GTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTCACCTTCCAGTGTGGCAACCTGCTGGCTGAGCGCTGGTTGTGCGATATGCAATACTTCAGCAGCCTGGGTCAGGCTACCAATATCTACAATTTTTACGAAGTATTTCAGGCGTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCATGC  >  NC_000913/2061757‑2062227                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               gTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTCACCTTCCAGTGTGGCAACCTGCTGGCTGAGCGCTGGTTGTGCGATATGCAATACTTCAGCAGCCTGGGTCAGGCTACCAATATCTACAATTTTTACGAAGTATTTCAGGCGTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGtt                                                                                                                                                                                                                              >  1:315854/1‑251 (MQ=255)             aaCGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTCACCTTCCAGTGTGGCAACCTGCTGGCTGAGCGCTGGTTGTGCGATATGCAATACTTCAGCAGCCTGGGTCAGGCTACCAATATCTACAATTTTTACGAAGTATTTCAGGCGTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGgtg                                                                                                                                                                                                                   <  1:165758/250‑1 (MQ=255)                              ggATCATAAGTCGTTGATTTAACTAAACTTCAAGTGAGGCAACCTGATGGCTGAGCGCTGGTTGTGCGATATGCACTACTTCAACAGCCTGGGTCAGGCTACCAATATCTACAATTTTTACGAAGTATTTCTGGCGTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGCATCGGCCCAGGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAAc                                                                                                                                                                                                  >  2:274102/1‑250 (MQ=255)                                                  aaCTCACCTTCCAGTGTGGCACCCTGCTGGCTGAGCGCTGGTTGTGCGATATGCAATACTTCAGCAGCCTGGGTCAGGCTACCAATATCTACAATTTTTACGAAGTATTTCAGGCGTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGTCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGGGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGAtt                                                                                                                                                                              <  1:371116/250‑1 (MQ=255)                                                    cTCACCTTCCAGGGTGGCAAACTACTGGCAGAGAGCTGGTTGTGCGATATGCAATACTTCAGAAACCTGGGTCAGGCTACCAATATCAACACTTTTTACGCAGTATTTCAGGCGTCTGAAGTTAATGTTGCCACCGGTTTTTAAGAATCGGCACAAGTGCCGCCATTAATTACAACCAGATCGAAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTACCCAACTGTAAAATAAGAGATTATg                                                                                                                                                                           >  2:440915/1‑251 (MQ=255)                                                                           gctgtctgACCGCTGGTTGTGCGATATGCAATACTTCAGCAGCCGGGGTCAGGCTACCAAGATCTACAATTTTTACGAAGTATTTCAGGCGTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTTCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCt                                                                                                                                                      <  2:69646/249‑1 (MQ=255)                                                                           gctggctgATCTCTGGTTGTGCGATATGCAATACTTCAGCATCCTGGGTCAGGCTACCAATATCTACAATTTTTACGAAGTATTTCAGGCGTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTGGCTCCtt                                                                                                                                                     <  1:313267/250‑1 (MQ=255)                                                                                    gcgcTGGTTGTGCGATATGCAATACTTCAGCAGCCTGGGTCAGGCTACCAATTTCTACAATTTTTACGAAGTATTTCAGGCTTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCAAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTg                                                                                                                                           <  2:195251/251‑1 (MQ=255)                                                                                             gtgCGATATGCAATACTTCAGCAGCCTGGGTCAGGCTACCAATATCTACAATTTTTACGAAGTATTTCAGGCGTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTcc                                                                                                                                  >  1:452979/1‑251 (MQ=255)                                                                                                                                      atatCTACAATTTTTACGAAGTAGTTCAGGCGTCGGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCAtt                                                                                         <  2:124333/251‑1 (MQ=255)                                                                                                                                                tttttACGAAGTATTTCAGGCGTCTGAAGTTCATGTTTCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATa                                                                                 >  2:143909/1‑249 (MQ=255)                                                                                                                                                    tACGAAGTATTTCAGGCGTCTGAAGGTCAGGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGAtt                                                                            <  1:238743/250‑1 (MQ=255)                                                                                                                                                                cAGGCGTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTcaccac                                                               <  1:342658/251‑1 (MQ=255)                                                                                                                                                                         gAAGTTCATGTTGCCTCCGGTTTT‑AAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTttgttg                                                        <  1:229799/248‑1 (MQ=255)                                                                                                                                                                               cATGTTGCCTCCGGTTTTTAAGAATCGGCGCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGAc                                                <  1:210599/251‑1 (MQ=255)                                                                                                                                                                                aTGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGAc                                                >  1:301494/1‑250 (MQ=255)                                                                                                                                                                                aTGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACa                                               <  1:314662/251‑1 (MQ=255)                                                                                                                                                                                     gCCTCCGGTTTTTAATAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGcaaa                                          <  1:181072/251‑1 (MQ=255)                                                                                                                                                                                                        cGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCAtt                        <  1:440915/250‑1 (MQ=255)                                                                                                                                                                                                        cGGCCCAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCAGTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATGGACAGCAATCAAACAAAAAAATGCAttt                       <  2:44759/251‑1 (MQ=255)                                                                                                                                                                                                           cccAAGTGCCGCCATTACTTACAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACAAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCCATAAGATTGAAAGCTCACCACTTTGTTGAAATTGAAAGCAAACAAACAAAAAACTGCATTTCAc                    >  2:231234/1‑251 (MQ=255)                                                                                                                                                                                                                             ttacAACCAGATCGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAGATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCATGc  >  2:431297/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               GTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTCACCTTCCAGTGTGGCAACCTGCTGGCTGAGCGCTGGTTGTGCGATATGCAATACTTCAGCAGCCTGGGTCAGGCTACCAATATCTACAATTTTTACGAAGTATTTCAGGCGTCTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGTATGCACCACGATGGTTCATTACCCAATAAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCATGC  >  NC_000913/2061757‑2062227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑