Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	350,292	G→A	R94H (CGT→CAT)	prpC →	2‑methylcitrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	350,292	0	G	A	97.7%	128.2 / NA	43	R94H (CGT→CAT)	prpC	2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/18);  minor base C (1/0);  total (25/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

AACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTG  >  NC_000913/350055‑350542                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                           aaCCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGa                                                                                                                                                                                                                                                >  1:153602/1‑250 (MQ=255) aaCCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCCAACGTGCATACCGTGCTGGa                                                                                                                                                                                                                                                >  1:228907/1‑250 (MQ=255)  aacGAAAAAATCTGTGGCCCTTCCTGGCGTTCCGGCGGGCAAGACTGCGCTCTTCACCGTGGGTAAAAGGGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAg                                                                                                                                                                                                                                              <  2:114731/249‑1 (MQ=255)  aCCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAACCATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAg                                                                                                                                                                                                                                              <  1:311433/251‑1 (MQ=255)        aaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTTCACCGTGGGTAAAATTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAATAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTTCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTAc                                                                                                                                                                                                                                        <  1:457546/251‑1 (MQ=255)                tgGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTATGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGCTATTCTTGATCTGGCGCAACCTTGCGAATTTGAAGAAGAGGCGCATCAGCTAATCCACGGAAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGCAAGCCCTGCGCGGTTTACCGGCTCACGTACATACCGTGCTGGAAGCCTTACCGgcggcg                                                                                                                                                                                                                                >  1:450835/1‑251 (MQ=255)                         cTGGCGCTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCg                                                                                                                                                                                                                       >  1:125155/1‑251 (MQ=255)                              gTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCgatgg                                                                                                                                                                                                                   >  1:106916/1‑250 (MQ=255)                                cccGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTGGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCgatggatg                                                                                                                                                                                                                <  2:318445/250‑1 (MQ=255)                                        gCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATgcgc                                                                                                                                                                                                        >  1:17528/1‑251 (MQ=255)                                          aaTACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCAc                                                                                                                                                                                                      <  1:134721/251‑1 (MQ=255)                                                                  aaaaGTGGCAATGACCAGCATTACCGCGGCTACTATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCAc                                                                                                                                                                              <  1:357381/251‑1 (MQ=255)                                                                          cAATGACCTGCCTTACCGCGGCTACAATATTCTTAATCTGGCGAAACATTGCGCCTTTGAAGAAGTGGCGCATCTGCTGATCCCCGGCAAACTGCCGACCCGTGACGCACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACCTGACTACCGTGCTGGTATCCTTACCGGCGGCGTCGCACCCGCAGGACGTTATGCGGACCGGAGTTCCCGCGCCCGGCTGCCAGCTGcccg                                                                                                                                                                      >  2:59646/1‑249 (MQ=255)                                                                                    cATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGAGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACACAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCCCCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAggg                                                                                                                                                            >  2:73960/1‑251 (MQ=255)                                                                                                           tGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCgg                                                                                                                                      <  1:271927/250‑1 (MQ=255)                                                                                                            gATCTGGAGAAACATTGCGAATTTGAAGAAGAGGCGAATCTGCTGATACACGGCAAACTGCCGACCCGTGAAGAACTCGACGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACAGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGAGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCggg                                                                                                                                     >  1:398777/1‑250 (MQ=255)                                                                                                                  gCGACACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACACAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGc                                                                                                                              >  1:331360/1‑251 (MQ=255)                                                                                                                   cGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATTCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGcc                                                                                                                             <  1:410176/251‑1 (MQ=255)                                                                                                                       aCATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCCCCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGAc                                                                                                                          >  1:298164/1‑250 (MQ=255)                                                                                                                             cGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACCGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAActgc                                                                                                                   <  1:394532/251‑1 (MQ=255)                                                                                                                             cGAATTTGAAGAAGGGGCGCATCGTCTGAGCCACGGCAAACTGCCGACCCGGTACGACCACGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTGCCGCGGTCGGCTGCACTCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGTGATATTGCCGACAAActgc                                                                                                                   <  2:181356/251‑1 (MQ=255)                                                                                                                                     aagaagTGGCGCATCTACTGATCCACGGCAAACTGCCACCCCGTGACAAACTCGCCGCTTCCAAAACGCAACAGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCCGGAAGACTTACCGGCGACGTCGCACACGCAGGCTCTTCTGCGCACCGGCGTTTCCGCGCACTGCTGCACGCTGCCAGACAAAGAGGGGCCTACCGCCGCTGGCCCGCGGGGTATAGCCGACAAACTGGTCGCGTCg                                                                                                           >  2:382692/1‑251 (MQ=255)                                                                                                                                          gTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGATGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAg                                                                                                      <  1:308524/251‑1 (MQ=255)                                                                                                                                           tGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTCACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGCTGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGc                                                                                                     >  2:204793/1‑251 (MQ=255)                                                                                                                                                 aTCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGAtt                                                                                               >  1:184428/1‑251 (MQ=255)                                                                                                                                                      ctgATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCt                                                                                          >  1:23316/1‑251 (MQ=255)                                                                                                                                                           ccACGGCCAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGACACTAAAAGCCCTGCGCGGTTTACCGGCTACCGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTAGCAACCGACGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGCAAAAGAGGGGCATCACGTCTCTGGCGCGCGGGATATTGCCGACAAACCGCTGGCGTCACCTAGCTCGCTTCTCCtttctt                                                                                     >  2:19947/1‑248 (MQ=255)                                                                                                                                                           ccACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCCGAAAAAGAGGGGCCTACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTAtt                                                                                     >  2:156463/1‑251 (MQ=255)                                                                                                                                                             aCGGCAAACGGCCGCCCGGTGAAGACCTCGCCGCTTAAAAAACTAAACTGAAAGCCCTGCGCGGTTTCCCGGCTAACGTGCATACCGGGCTGGAAGCCTTACCGGCGGAGTCGCACCGGATGGATGTGATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCGGCCAGAAAAAGAGGGTCATACCGTCTCTGGCGCGCGGGATATTGCCGACAATCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTgg                                                                                   <  2:21309/251‑1 (MQ=255)                                                                                                                                                                             ccGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCcaca                                                                   <  1:90392/251‑1 (MQ=255)                                                                                                                                                                             ccGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCcac                                                                    >  1:163683/1‑250 (MQ=255)                                                                                                                                                                                 gACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGTGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTAGTGGTATCACTACAGTCACAACg                                                                <  1:334429/250‑1 (MQ=255)                                                                                                                                                                                      aCTCGCCGCTTACAAAACGAAGCTGAAATCCCTGCGCGGTTTACCGGCTTACGTGCATACCGGGCTGGAAGCCTTACCGGCGGCGTCGCACCCGTTGGATGTTATGCGCACCGGTTTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCGGGCGCTCGGGATATTGCCGACAAACTGCTGGCGGCGCTTAGCGCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAAc                                                          <  2:286140/251‑1 (MQ=255)                                                                                                                                                                                             gcTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATcc                                                    >  1:387141/1‑250 (MQ=255)                                                                                                                                                                                                     aaCGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGTGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGaa                                            >  1:48415/1‑250 (MQ=255)                                                                                                                                                                                                         aaaCTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCg                                        >  1:37946/1‑250 (MQ=255)                                                                                                                                                                                                          accggAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATCCCGTGCTTGAAGCCTTACCGGCGGCGGCGCCCCCGGTGGATGTTATGCGCACCGTGGGTGCCGCGCTCGGCTGCACGCTGCCATAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGTTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTGCAGCCACAGCGGCGAGCGCATCCAACCGGAAACCGAt                                      <  2:367422/247‑1 (MQ=255)                                                                                                                                                                                                          aaCTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGAt                                      >  1:396357/1‑251 (MQ=255)                                                                                                                                                                                                                   cccTGCGCGGTTTACCGGCTAACGTGCATTCCGTTCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGGCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTcc                             <  2:430755/251‑1 (MQ=255)                                                                                                                                                                                                                    tcagcgcGGTTTACCGGCTAACGTGCATACCGTGCTGGCAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGTAAAAGAGGGGCATACCGTCTCTGGCGCGCAGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCATTTATTGGTATCACCACAGCCACAACGGCGAACGACTCCACCCGGCAACCGATGACGCCTccc                            >  2:68530/4‑250 (MQ=255)                                                                                                                                                                                                                              ttACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTc                   >  1:425962/1‑250 (MQ=255)                                                                                                                                                                                                                               tACCGGCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCa                  <  1:375749/250‑1 (MQ=255)                                                                                                                                                                                                                                    gCTAACGTGCATACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATcggcgggccactccc            >  1:239150/1‑242 (MQ=255)                                                                                                                                                                                                                                              gTACCGTGCTGTAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGCCACCTcccctctctcccc  >  2:117519/1‑240 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                           AACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTG  >  NC_000913/350055‑350542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑