Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,751,849	G→A	D19N (GAT→AAT)	recN →	DNA repair protein RecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,751,849	0	G	A	100.0%	104.3 / NA	32	D19N (GAT→AAT)	recN	DNA repair protein RecN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/12);  total (20/12)

ATTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATG  >  NC_000913/2751608‑2752095                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       aTTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCAt                                                                                                                                                                                                                                                >  2:350092/1‑250 (MQ=255)                 aCCAGCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCAACACATTAAGCGCCAAGCTCGGCTGGT‑CAAAAAAAGTATTCTAATTTTTCGCCAGCCTTTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCGGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGtaa                                                                                                                                                                                                                                <  1:194512/250‑1 (MQ=255)                              taTTCAACCGAAAGATTACAGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGT‑CAAAAAAATTATGCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACATGAAAACGACTATGTTGGCACAACTGACCATCAGCAACGTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTACTAACTgtcgtgac                                                                                                                                                                                                                   <  2:116966/249‑8 (MQ=255)                                       gAAAGATTACAGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCCACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAACGGCATGACCGTATTACCTGGCGAGACCGGCGCGGGt                                                                                                                                                                                                         >  2:172883/1‑251 (MQ=255)                                         aaGATTACAGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTa                                                                                                                                                                                                        >  1:254861/1‑250 (MQ=255)                                               aCAGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACAGTACACAATAAAAGAAATGGTTCTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTGCGAGAGCTGGAGAGTAATTTGCATAGCGGCATGACCGTAAGCACTGGCGAGAACGGCGCGGTTCAATCTAt                                                                                                                                                                                                 >  2:67899/1‑251 (MQ=255)                                                 aGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACAAAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTATCATAGCGGCATGACCGTAATAACTGTCGAGACCGGCGCGGGTAAATCTATTg                                                                                                                                                                                               >  2:122508/1‑251 (MQ=255)                                                   ttATTTCAACACATTAAGCACCAAGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGcc                                                                                                                                                                                             >  2:175972/1‑250 (MQ=255)                                                      tttCAACATATTAAGCACCAAGTTCGAC‑GGTGCAAAAAAAGCATTTTAATTTTACGCCAGCCTCTTTCCTGTATATTAAGCCATATTATTCTGTTCACAATTACAGTAATGGTTTGTCATACAGTTAAACGTCTATGGTGGCACATCTGCCCATCATCAACTTTTCTATCGTACGTGAGCATGAGATTAATTTTCATAGCGGAATGTATGTAATAACTGGCGAGACCGGCGCGGGTAAATCTAATGCAAt                                                                                                                                                                                           <  2:432982/250‑1 (MQ=255)                                                              caTTAAGCACCAAGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCt                                                                                                                                                                                  <  1:369208/251‑1 (MQ=255)                                                              caTTAAGCACCAAGCTCGGCTGGT‑AAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACCACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCt                                                                                                                                                                                  >  2:207082/1‑251 (MQ=255)                                                                        cAAGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTATTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGt                                                                                                                                                                        >  1:67625/1‑251 (MQ=255)                                                                         aaGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGTCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGGAAATCTATTGCAATAGATGCCCTCGGTCTTTGtc                                                                                                                                                                       <  1:438778/251‑1 (MQ=255)                                                                          aGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGtct                                                                                                                                                                      >  1:362065/1‑251 (MQ=255)                                                                              cGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCggg                                                                                                                                                                  >  1:242451/1‑250 (MQ=255)                                                                              cGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGACTTTGTCTCggt                                                                                                                                                                  >  2:260108/1‑251 (MQ=255)                                                                                   ggT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCCTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTCTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCCACTTTGCTATCGTTCGTGAGATTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGGCAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTcg                                                                                                                                                             >  1:183132/1‑251 (MQ=255)                                                                                           aaaaTTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGaa                                                                                                                                                      <  1:166294/251‑1 (MQ=255)                                                                                                               ccAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAAGTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTGTGTCTCGGGGGTCGCGCTGAAGCCGACATGGTGCGTACCgg                                                                                                                                  <  1:207082/251‑1 (MQ=255)                                                                                                                 aGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGgcg                                                                                                                                >  1:301828/1‑251 (MQ=255)                                                                                                                   ccTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCgct                                                                                                                              >  1:6121/1‑251 (MQ=255)                                                                                                                          aCTGTATATAAAACCAGTTTATACTGTACACAAAAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGAAAATCTATTGCAATAGATGCCCTCGGGCTTTGTCTAGGTGGCCGCGCTGAAGCCGACACGGTGAGTACCGGCGCTGCTcgcg                                                                                                                       >  1:406952/1‑251 (MQ=255)                                                                                                                                 ataAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGAcc                                                                                                                 <  1:439969/250‑1 (MQ=255)                                                                                                                                 ataAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGAcc                                                                                                                 <  1:78247/250‑1 (MQ=255)                                                                                                                                            ttATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCCTGACACCGACATGGCTCGTACCGGCGCTGCTCGCGCGGTCCTGTGCGTCCGtt                                                                                                     >  2:88264/1‑251 (MQ=255)                                                                                                                                                  tGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtctc                                                                                               >  1:84267/1‑251 (MQ=255)                                                                                                                                                     acacAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGa                                                                                            <  1:190410/251‑1 (MQ=255)                                                                                                                                                                                cAGGAAAACGACTCTGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGCGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGCAAGATACGCCAGCGGCTCTGCgctggc                                                                 >  1:179927/1‑251 (MQ=255)                                                                                                                                                                                 aGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCgctggct                                                                >  1:447065/1‑251 (MQ=255)                                                                                                                                                                                            tATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTAGCGAGACCGGCGCGGGTAACTCTATTGCATTAGATGCCCGCGGGCTTTGTCTCGGTGGCCGCGCTAAAGCCAACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGATTTTCTCTGACAGCTACCCCAGCGGCTCTGCGCTGGCTGGAAAAAAAcc                                                     >  1:52739/1‑251 (MQ=255)                                                                                                                                                                                                     acaACTGACCATCAGCAACTTTGCTATCGTTCGTTAACTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGCCCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAg                                            <  1:326322/251‑1 (MQ=255)                                                                                                                                                                                                                  aGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAAGAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTTCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCTTGAATg                               <  1:162451/251‑1 (MQ=255)                                                                                                                                                                                                                                               gattaattTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGGCGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATg  >  1:111790/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       ATTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGT‑CAAAAAAATTATTCTAATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATG  >  NC_000913/2751608‑2752095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑