Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,878,434	G→A	intergenic (+816/+135)	iap → / ← cas2	alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,878,434	0	G	A	96.9%	91.5 / NA	32	intergenic (+816/+135)	iap/cas2	alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (12/19);  minor base T (0/1);  total (12/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAGCGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCC  >  NC_000913/2878199‑2878680                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                        cccGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCACGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCt                                                                                                                                                                                                                                          >  1:355240/1‑251 (MQ=255) cccGATGGCGCGGTGAAACCCCGACGGCGCAGAAGTTCCGGACACGATCCCAACGGTTCCTCCCCGCTGGCGCGGGGACCGCCGAACGGTATCAATTACAGCCGCCAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCGGCGAAAACGGTTTATCCCCGCt                                                                                                                                                                                                                                          <  2:298700/251‑1 (MQ=255)                       gaccggttggaacttCCAGCCGCGATCCATACGGTTTATCCCCGCGGGCGCGGGGATCGCTCAACATTATCAAGTACCACCGGCAGGGAGCCCGGGTTATCCCCGCTGGCGCGGGGATCTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGTGCGTGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAATCGGTGTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAg                                                                                                                                                                                                                   <  2:20761/238‑1 (MQ=255)                        cccGCTCAGAATTTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGTGAGCCCGGTTTATCCCCTCTGGCGCGGGGAACTCAGCGTGTTCGGCATCGCCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGt                                                                                                                                                                                                                  <  2:285895/250‑1 (MQ=255)                        aCCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGt                                                                                                                                                                                                                  <  1:311543/251‑1 (MQ=255)                          cGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGtat                                                                                                                                                                                                                <  1:254883/251‑1 (MQ=255)                           gCTCATAAATTCCAGACACGATCCAAACGGTTTACACCCGCCGGCACGGCGAAATCTCAACCTCATCCATTACAACCGACAGGGAGCCCGGTTTATCCACGAAAGCGCGGGGAACTCAGCGTGATCGGCATACCCTTTGGCTTCGGCTGCGGTTTATACCCGCTGGCGCGGGGAACTCAGCGCGAGCGTATCGCCGCACGTCTGCGAAAACGAGTTATACCCGCTA‑GCGAGGGGAActctccacaactaa                                                                                                                                                                                                                >  2:186722/1‑241 (MQ=255)                              cAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACa                                                                                                                                                                                                             <  1:22824/250‑1 (MQ=255)                                                                ccGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACTGACAGTGAGCCCGGTTTGTCCCCGCTGGCGCGGGGAACTCAGCGGGTTCGGCATCGCCGTTGGCTTCGGCTGCGGGTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTAGCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGGTCTTAAAGCATTGTTTCCCATAAAgacaac                                                                                                                                                                          <  2:190746/251‑1 (MQ=255)                                                                ccGCGGGCGCGGGGAACTCCCAACATTATCAATTGCATCCGGCAGGTGGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAaacaac                                                                                                                                                                          <  2:16564/251‑1 (MQ=255)                                                                  gCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGG‑AACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAAccc                                                                                                                                                                        >  1:80148/1‑250 (MQ=255)                                                                        gcgGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACAGTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAAc                                                                                                                                                                  <  1:110240/251‑1 (MQ=255)                                                                           gggAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCCTTTTTTCCCATAAAAACAACCCACCAACCtt                                                                                                                                                               >  2:57164/1‑251 (MQ=255)                                                                                             cAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATACAACATTTCCtatt                                                                                                                                             >  2:57503/1‑248 (MQ=255)                                                                                               aTTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCttatta                                                                                                                                           <  1:65700/251‑1 (MQ=255)                                                                                                  aCAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCACCGTCTTAAGCATCACCTTTGGCTTCGGCTGCGGCTTATCCCCGCTGGCGCGGGGAACTCTGCGTGCGCGTATCGCAGCGCGCCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTATCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTCACATTTCCttattatt                                                                                                                                         >  1:184188/1‑250 (MQ=255)                                                                                                     aCCGACAGGGCGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAg                                                                                                                                     <  2:79147/251‑1 (MQ=255)                                                                                                     aCCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAACGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTCTTaaa                                                                                                                                      >  2:344039/1‑250 (MQ=255)                                                                                                      ccGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCCTTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTaa                                                                                                                                       >  1:297543/1‑248 (MQ=255)                                                                                                               ggCCCGGTTTAGCCCCGGTGGCGCGGGGAACGCAGCGTGTTCGGCATCCCCTTTGGCGTGGGCTGCGGTTTATCCCCGCGTGCGCGGGGAACTCTGAGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCG‑TGTGTGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACACACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCGAAt                                                                                                                           <  2:115438/250‑1 (MQ=255)                                                                                                                        gattcccgatggctgggggAACTCCGCGTTGTCGGGCTCACCTTCTGCTGCGGCTGCGGTTTCTCCCCGCTGGAGCGGGGAACTCTGCGTGAGCGGATCGCCGCTCGTCTGCGATAAAGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCACCCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGtt                                                                                                                   <  2:238943/235‑1 (MQ=255)                                                                                                                         aTCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGtttt                                                                                                                 <  2:89214/251‑1 (MQ=255)                                                                                                                           ccccGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCCCCTTTGGCTTCGGCTGCGGTTTACCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTc                                                                                                                <  1:103980/250‑1 (MQ=255)                                                                                                                                  ggCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCACCCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGt                                                                                                        <  1:342256/251‑1 (MQ=255)                                                                                                                                                                        ttCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAACCCATCCAAATCTACCgg                                                                  >  2:193572/1‑251 (MQ=255)                                                                                                                                                                                 ggTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGATCGTATCGCCGCGCGTCTTCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGTGTACGCCt                                                         <  2:116687/251‑1 (MQ=255)                                                                                                                                                                                              tGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAAt                                            >  1:157817/1‑251 (MQ=255)                                                                                                                                                                                                      ggAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGaa                                    <  1:193572/251‑1 (MQ=255)                                                                                                                                                                                                                gTGAGCGTATCGCCGCGCGTCTGCGAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATccc                          <  1:307415/251‑1 (MQ=255)                                                                                                                                                                                                                    gCGTATCGCCGCGCGTCTGCGAAAACTGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGttt                      <  2:157817/251‑1 (MQ=255)                                                                                                                                                                                                                       tATCGCCGCGCGTCTGCGAAAACGGTTTATCCCAGCTG‑ACGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAGACAACCCACCAACCTTAATGAAACATTTCCTTATTATTAGAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGAACGCCTGTTTAACCAAAATGTCTGGAACTCAAATCCCGTTTCCg                   >  1:178047/1‑251 (MQ=255)                                                                                                                                                                                                                                        gAAAACGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGcc  >  1:269917/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                        CCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCTGCGGTTTATCCCCGCTGGCGCGGGGAACTCTGCGTGAGCGTATCGCCGCGCGTCTGCGAAAGCGGTTTATCCCCGCTG‑GCGCGGGGAACTCTCTAAAAGTATACATTTGTTCTTAAAGCATTTTTTCCCATAAAAACAACCCACCAACCTTAATGTAACATTTCCTTATTATTAAAGATCAGCTAATTCTTTGTTTTCAAACAGGTAAAAAAGACACCAACCTTAAACCATCCAAATCTACCGGGGTACGCCTGTTTAACCCAAATGTCTGGAACTCAAATCCCGTTTCCGTATTCGTTGCCCATGCC  >  NC_000913/2878199‑2878680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑