| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,173,363 | Δ2 bp | intergenic (‑490/+918) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,363 | 0 | C | . | 85.7% | 35.5 / 3.1 | 15 | intergenic (‑490/+919) | gatD/gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (9/3); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
| * | NC_000913 | 2,173,364 | 0 | C | . | 80.0% | 32.4 / 6.3 | 15 | intergenic (‑491/+918) | gatD/gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base . (9/3); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
TTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGA‑TTAAGGTGCGGAACAGATTTCCACG‑ATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCC > NC_000913/2173126‑2173598 || ttAGCCGCCCGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGA‑TTAAGGTGCGGAACAGATTTCCACG‑ATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTccc > 1:330098/1‑249 (MQ=255) caccaGGGGGATGCCCATAATGA‑GGCCCCCGAGAATGAAGGTGGGGAACA‑ATTTCCCCGGAGGCACGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGCAATACCGCCAAACGGCGGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGAAAAAAAATCAGGCTGGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGa < 2:417765/248‑1 (MQ=255) acaGGGTGATGCTCATAATGATGACACCCGAGA‑TTAAGGTGCGGAACAGATTTCCACG‑ATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAc > 1:139585/1‑251 (MQ=255) atgatgACACCCGAAA‑TTAAGGTGCGGAACAGATTCCCACG‑ATGCCCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAGACGACt < 1:119943/251‑1 (MQ=255) aaCAGATTTCCACG‑ATGCACGGCGACCGCCATCGCCACGAAGACGCCGCTGGTGGCAAGATCGCCACACGGCAGCACCTGATTACCCGGCACACACACAGCAATTAAAATGGTGAGCGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAAGGGCGTTa > 1:276270/1‑250 (MQ=255) gaagaaGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACAAAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagc > 1:251020/1‑250 (MQ=255) aaaTCGCAAAACGGCGGCACCTGATTACCCGGAACACAAACAGCAATAAAAATGGTGAGGGGAAAAAAAATCAGGCTTGCCGAT‑CC‑CGCCGCCTTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGtttt < 1:46994/249‑1 (MQ=255) aTCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGCAAGTAAAATGGTGGGTGGGATAAAAACCAGGTTTCCCGATAC‑‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGTGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAGCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTa < 1:419407/251‑1 (MQ=255) aaCGGCACCACTTGTTTCCCCGGCACAAAAACTGAAATTAAATTGTTGGGGGAGAAAAAAATCGGGCTTACCGAAACCACCTC‑CGGT‑GGTCCCAGCTGCACCGCCGGATCAAGGCCAATCAGGAACTCCGGACCGCCGATCTTCGCCTGTAAACGACTACGAGCCTGCTTATCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCTTTGCATTAGCAGCAGCACTGCCGCCGTTTTTACCGCCAg < 1:59796/251‑1 (MQ=255) gcaCCTGATTACCCGGCACACAAACAGCAATAAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCCTTAGCAGCATCCCTGCCGCCGTTTTTACCGCCAGCTGCAAt > 1:34336/1‑251 (MQ=255) gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCt > 1:44765/1‑251 (MQ=255) tacatccAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTAGCCGATACCAAC‑‑GCCGTATGTCACAGCAGCAACGCCGGAGCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACCAGCCTGCTTAGCGGTGGGCGTTAAACAATACATGATGGGTTTAATCACCCGTTGCATTAGCAGCATCACTGCCGCCGGTTTTACCGCCAACTGCAATACACCTTTGACATCGTa > 2:352151/7‑250 (MQ=255) acaAACAGCAATTAAAATGGTGAGGGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTGGTCCGCCAGCTGCAATACACCTTTGACATCGTAAc < 1:67008/251‑1 (MQ=255) gTGGGATAACAATCCGGCTTGCCGAAACCAAC‑‑GCCGTATGTCCCAGCAGCAAAGCAGGATCAAGGCAAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAACCGCCTACGAACCTGCTTAGCGATGGGCGTTACACCATCCATGCTGGGTTTAATCACCCGAGGCATTAGCAGAATCACTGCCGCCGTTTTTACCGCCAGCAGCACTACACCTTTGACCTCGTCACCCGCGAGAATGCCGATCATCAGc > 2:67637/1‑251 (MQ=255) gCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGCTGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAccc > 1:148794/1‑250 (MQ=255) || TTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGA‑TTAAGGTGCGGAACAGATTTCCACG‑ATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCC > NC_000913/2173126‑2173598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |