Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,656,658	G→A	A159T (GCC→ACC)	speG →	spermidine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,656,658	0	G	A	90.9%	57.0 / ‑6.1	23	A159T (GCC→ACC)	speG	spermidine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (9/11);  minor base C (1/1);  total (10/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAA  >  NC_000913/1656421‑1656903                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      gTTCATCGACGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCAGCCAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACAGCAAGCTTGGCTTTTCGGTTGCAGGAGAATTGATGCACGAGTTCTTTCTTAATGGTCAATATCGTAATCCCATTCGCAtgtg                                                                                                                                                                                                                                          >  2:105931/1‑251 (MQ=255)       cgccgtgaaGAATTTCAGCTAATTATCTCCCCGGCGTATCAGGGGAAAGGTCTGGCAACCCGTGCAGCCAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATAtt                                                                                                                                                                                                                                    >  2:308729/9‑251 (MQ=255)         ccgcGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTcc                                                                                                                                                                                                                                  <  1:395722/251‑1 (MQ=255)                                     ccGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAac                                                                                                                                                                                                      <  1:37/251‑1 (MQ=255)                                                     aaGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACtctc                                                                                                                                                                                      >  1:425917/1‑251 (MQ=255)                                                                       ccgccAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGcaca                                                                                                                                                                     >  2:397265/1‑250 (MQ=255)                                                                          ccAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGTAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAg                                                                                                                                                                 <  1:23245/251‑1 (MQ=255)                                                                                              ggCTTTACCGTTCTCAATCTCTATAAGCTGTCTCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGt                                                                                                                                             >  1:39561/1‑251 (MQ=255)                                                                                                       gTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGCTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGAt                                                                                                                                    >  1:150802/1‑251 (MQ=255)                                                                                                           ccAATCTCTATAAGCTGCATCTGATCGTTGATAAAGAGACTGAAAAACCGAATACCATGTTCCGCAAGCTTGGCTTTTCGGTTGAAGGTTAATTGATGCACGATTTTGTTATTAATGGTCAATATCGTAATCCCATTCGCATGTGTATATTCCAGCATCAGTATCTTGCAAAGCATAAAACCCCGGTTCAGACTCACCTGAAGCCTATCGCTCAATTTCATTAATAATAATCGATCGTAtttatgtttgtg                                                                                                                                <  2:341802/250‑10 (MQ=255)                                                                                                                    ataAGCTGTATCTGATCGTTGATAAAGAGAAGGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGGATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAAcc                                                                                                                          <  1:36167/248‑1 (MQ=255)                                                                                                                                                    gAAAAAGCGATTCCCATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATGTATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTTTATATTCCATCATCAGTATCTGGCTGAGCACAAAACACCGGGTCAGACTATCCTGAAGCCTACCTCACAATATCATTAATAATAATCGATCGTATTATTGAGGGTGTAAACCCGTTTGACTGCGGGTTTTACTCCTTCATcaacaa                                                                                        <  2:454073/250‑1 (MQ=255)                                                                                                                                                         aGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTa                                                                                  <  1:65663/251‑1 (MQ=255)                                                                                                                                                               tCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAAGACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGa                                                                             <  2:126920/250‑1 (MQ=255)                                                                                                                                                                cacttttACCGAAAGCTTGGCTTTTCGGTTGAAGGTTAATTGATGCACGAGTTCTTTATTAATGGTCAGTATCGTAATTCCATTCGCATGTGTATATTCCTGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGGTTTACTCCTTCATCAACAATGATCAGCTGACa                                                                           <  2:244860/247‑1 (MQ=255)                                                                                                                                                                     ttACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGTGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCTATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTGTTACTCCTTCAGCAACAATGATTAGCTGACAGTcc                                                                       <  2:39561/250‑1 (MQ=255)                                                                                                                                                                                  ggCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCATTATCTGGCAGAGCACAAAACACCTGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACTGCGGGTTTTACTCCGTCAGCAACAATGATTATCTGACAGGCCACCGGATTAGCGt                                                          <  2:400477/250‑1 (MQ=255)                                                                                                                                                                                                              aCGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACAGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTAc                              >  1:403897/1‑250 (MQ=255)                                                                                                                                                                                                                  gTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATATGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCACATTCACAGCTCTGTTTTACATTAc                         >  2:64721/1‑251 (MQ=255)                                                                                                                                                                                                                       ttattaATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTACATTACCAAc                     <  1:397265/250‑1 (MQ=255)                                                                                                                                                                                                                               ggTCAATATCGTAATACCATTCGCATGTGTATATTACAGCATCAGTAACTGGCAGAGCACAAAACACAGGGTCAGACTCTACTGCAGCAGACCGAACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGGTTAGCTGACAGTCCACAGGATTAACGTGAATGTCATATTCAAAGCTCTGTTTTACATTACCAACCCGATGatt            >  1:397757/1‑251 (MQ=255)                                                                                                                                                                                                                                 tCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGattat          >  1:442264/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTAATACCATGCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAAGCGATCGTATTTTTGATGGTGTAATCCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTTACATTCCACCGGATTAGCGTGAGTGTCAGATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCagtaaa  <  1:261746/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      GTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAA  >  NC_000913/1656421‑1656903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑