Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,477,804	C→T	L62F (CTT→TTT)	ynbA →	CDP‑alcohol phosphatidyltransferase domain‑containing protein YnbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,477,804	0	C	T	100.0%	43.8 / NA	20	L62F (CTT→TTT)	ynbA	CDP‑alcohol phosphatidyltransferase domain‑containing protein YnbA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/12);  total (8/12)

AGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGATTAACGGTG  >  NC_000913/1477592‑1478035                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        aGCGCAAACAGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATg                                                                                                                                                                                                   <  1:421533/251‑1 (MQ=255)             aTTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTg                                                                                                                                                                                         >  1:362755/1‑249 (MQ=255)                      gATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGATGTTAAGGCCGACGATGTGTTGGCTTTATAAGCACCACGTTACAGCGACTCACCTCACTCTTGCTGCACTGGCGCTTTCTCTTATTACCGGCTTGCTGTTGATGTTGGCGGCGAAAGCCATTCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGAGCAAcc                                                                                                                                                                                >  1:193692/1‑249 (MQ=255)                                                     gCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATgaga                                                                                                                                               <  1:20098/251‑1 (MQ=255)                                                                 gcggTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCGGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTa                                                                                                                                   <  2:20353/248‑1 (MQ=255)                                                                      tAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTTATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGTCGCTCAATGCGCTGGATGGCAGGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATTTTATTTc                                                                                                                               <  2:446134/250‑1 (MQ=255)                                                                             gacgaTGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTg                                                                                                                       <  2:362755/251‑1 (MQ=255)                                                                                         gtgttATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCTGATTGCTGTTGATGTTGGCTGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCTTATGGCGCTCAATGCGCTTGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTAGTTCCGATATTGCGCTCTATTTa                                                                                                            <  2:174987/247‑1 (MQ=255)                                                                                               taAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttt                                                                                                     >  1:156488/1‑251 (MQ=255)                                                                                                  gCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttat                                                                                                  <  1:334529/251‑1 (MQ=255)                                                                                                                       catcacTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCAt                                                                             >  1:323461/1‑251 (MQ=255)                                                                                                                              ctTGCTGCACTGGCGCTTTCTCTTCTTTCCGGATTGCTGTGGATGTTGTCGGCACATCCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGGAACCAGCAAAGACGTCTGGGGGCGATTTTGAATGAGACTGGTTATGTTATTTCCGATATTGCTTTCTATTGACCATTTTTATTTTTACCGGAAAGTAACGCATCACTc                                                                        <  1:200214/249‑1 (MQ=255)                                                                                                                                    gcACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTTGGGGCGATTTTTAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTTCCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGAt                                                                   <  2:251893/248‑1 (MQ=255)                                                                                                                                                     tcttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCAt                                                                                                                                                                                                    >  1:358388/1‑102 (MQ=255)                                                                                                                                                     tcttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCAt                                                                                                                                                                                                    <  2:358388/102‑1 (MQ=255)                                                                                                                                                                             ggcggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTAc                       >  1:375287/1‑251 (MQ=255)                                                                                                                                                                                     aaCCCATCCTCTTTTTGCTATTGCCCATCGTGTTTTTTATACGTATGGCGCTCAATGCGCTGGATGGCCTGTTGGCGCGTGAGTGCAAACAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTTCGGTTTACTCgcgc                 >  2:365455/1‑249 (MQ=255)                                                                                                                                                                                              tctTTTTGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGATTAAc      >  1:330659/1‑251 (MQ=255)                                                                                                                                                                                                 ttttGCTAATGCCCAGCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCCACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCTATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGTAAAGTAACGCATCACTCGTGATACTCATGTGAGTTTGCACCACATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGATTAACgg    <  2:286241/250‑1 (MQ=255)                                                                                                                                                                                                   ttGCTATTGCCCATCGTGTTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTGGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATGGACCGATTTTTGCGGTTTACTCGCGCAGACGATTAACGgtg  <  1:407189/250‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        AGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGATTAACGGTG  >  NC_000913/1477592‑1478035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑