Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,578,759	G→A	I193I (ATC→ATT)  I193I (ATC→ATT)	mcrB ← mcrB ←	5‑methylcytosine‑specific restriction enzyme subunit McrB 5‑methylcytosine‑specific restriction enzyme subunit McrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,578,759	0	G	A	100.0%	34.3 / NA	12	I193I (ATC→ATT)  I193I (ATC→ATT)	mcrB mcrB	5‑methylcytosine‑specific restriction enzyme subunit McrB 5‑methylcytosine‑specific restriction enzyme subunit McrB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

TGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCC  >  NC_000913/4578528‑4578986                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    tGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGAGt                                                                                                                                                                                                                   >  1:182593/1‑250 (MQ=255)                ttGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGCACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGTGTATCGTCTCTATTGTgg                                                                                                                                                                                                   >  1:6668/1‑251 (MQ=255)                      aCAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTc                                                                                                                                                                                               >  1:214434/1‑249 (MQ=255)                        aaaaaTTGTAAAATATGCCGTCTTTACGTCAGAAGCCGACGCCATTAGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTAACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAggg                                                                                                                                                                                           >  1:434329/1‑251 (MQ=255)                                                     cGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAg                                                                                                                                                              <  1:363351/251‑1 (MQ=255)                                                            cgacgaCATTCTGACGATAGCCCTGTATAAAATCCTCATATCTATAAGATTGATGTAACGGAACCATATGGACTCGTTGAGGAGCCTGTTCTCCTTGCAGCAAGTAAGCCAGACGGCGTGCAACAAAGTTTTTGCCAACGCCGGGTGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACATTAt                                                                                                                                                       <  2:343849/251‑1 (MQ=255)                                                                                                    gCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCATGCCAg                                                                                                               <  1:120275/251‑1 (MQ=255)                                                                                                                          aCCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTc                                                                                         <  1:435265/251‑1 (MQ=255)                                                                                                                                               gCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCAt                                                                    <  1:267832/251‑1 (MQ=255)                                                                                                                                                                       gCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGAGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCATAGAGGCAAATCGGGTGAAATCAAt                                            >  1:33856/1‑251 (MQ=255)                                                                                                                                                                                cGTGCAACAAAGGATTTTCCACCGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTAATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGTGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGTAGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCATAATGGCAAGTCGGGTGTAATCAATACCTtgtg                                    >  2:98669/1‑250 (MQ=255)                                                                                                                                                                                                                   cTGGATGATCATATGTTTTTTAATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGTGATATACAAATCATTTAACGCATCTGCCAGACAGTATGATTCATTTTTTGTCATAGGTGGAATTATACTCTTGCCAGAATTAAATATGAATTTAGAGTCGTTGATCATGTTGTACAGCCTAGTAGCCAATCCGCAGTATTCATTACCCTGTGAGACTTTTTGGGAATAGCCGTAATAGGACTGTcc  <  2:115512/249‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    TGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCC  >  NC_000913/4578528‑4578986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑