Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,633,668	T→A	intergenic (‑1078/‑1173)	yhiJ ← / → yhiM	DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,633,668	0	T	A	100.0%	104.2 / NA	37	intergenic (‑1078/‑1173)	yhiJ/yhiM	DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (22/15);  total (22/15)

GATTTCCAGCCAGAACGACGACATTTTTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTAT  >  NC_000913/3633426‑3633899                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        gATTTCCAGCCAGAACGACGACATTTTTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGt                                                                                                                                                                                                                                 >  2:8187/1‑251 (MQ=255)      ccagccagAACGACGACATTTTTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGt                                                                                                                                                                                                                             >  2:99491/1‑250 (MQ=255)                   cgacATTTTTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAATATCATGTCTCTCGTATTTAATATATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGcc                                                                                                                                                                                                               <  2:191184/251‑1 (MQ=255)                         ttttATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATctct                                                                                                                                                                                                         >  1:360991/1‑251 (MQ=255)                           ttattaATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCtt                                                                                                                                                                                                        <  2:336595/250‑1 (MQ=255)                                aaTACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCgtgt                                                                                                                                                                                                   <  1:245914/250‑1 (MQ=255)                                         atatttATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAAtt                                                                                                                                                                                         <  2:347279/248‑1 (MQ=255)                                                    cctacATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATCTAATAAATCGTAGAGCACTTATTTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACAtattaa                                                                                                                                                                               >  2:235278/5‑250 (MQ=255)                                                                gattAGAATTTAGAAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATATCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCttt                                                                                                                                                                  >  1:245999/4‑251 (MQ=255)                                                                gCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCttt                                                                                                                                                                  >  1:246002/1‑251 (MQ=255)                                                                  aTAGAACTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTATTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTc                                                                                                                                                                >  1:123231/1‑251 (MQ=255)                                                                              aaaGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCtat                                                                                                                                                    >  2:334565/1‑251 (MQ=255)                                                                                       atatatTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGa                                                                                                                                           <  1:99491/251‑1 (MQ=255)                                                                                         atatTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGGCTCTCGTATTTAATAAATCGTAGAGCGCTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAAGAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGaa                                                                                                                                          <  1:257533/250‑1 (MQ=255)                                                                                              tATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGtt                                                                                                                                                                                                                                <  1:444987/159‑1 (MQ=255)                                                                                              tATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGtt                                                                                                                                                                                                                                >  2:444987/1‑159 (MQ=255)                                                                                                tCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCa                                                                                                                                   >  2:164065/1‑250 (MQ=255)                                                                                                  aCCAAAAATAGAACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATAAAAGGGGTTATCGCCTCGTCCTTTAAACAAAAATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGAAGATGAAGCCATCTCTTCGTGTTTTAATAATTAACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCactg                                                                                                                                >  2:264083/1‑248 (MQ=255)                                                                                                           aGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGt                                                                                                                        >  2:216407/1‑250 (MQ=255)                                                                                                            gTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATCCATGGGGTTCTCGCATCCTCCTTTAAAAAAAAATGTTGATGCAACTAATCCAACATCATGTCTATCGTATTTAATAAATCGTCGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCATGATGCCTATATGCCCTGAAACGAGCAATGCATAAAgcac                                                                                                                      >  1:208708/1‑248 (MQ=255)                                                                                                            gTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGGTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTATAGCTTTTGATTGATTTTATGTTTGTATGCAGATGAGGCCATGTCTTCGTGTTTTAATAATTCACATAATAAGCGCCAACGCTTTTCCCGTATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTAc                                                                                                                      <  1:46544/251‑1 (MQ=255)                                                                                                                       aTCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTActc                                                                                                           >  1:239998/1‑251 (MQ=255)                                                                                                                            cTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTcc                                                                                                       >  1:320418/1‑250 (MQ=255)                                                                                                                                  gATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGt                                                                                                 <  1:8187/250‑1 (MQ=255)                                                                                                                                                                    gTCCTTTAAATAAACATGTTGATGCAACTCATTCAAGATCATGTCTCGCGTATTTAATAAATCGTAGAGCACTTTGTTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAAGTCACATTTTATACTCCAACCCGTTGCACTGATGCCTATATGCCTTGAAACGAGCGAGGCGTATAGTACGGCGGCGATTCTTCGGTCTGTAGCGCCAAAATAAGCGGTGTTGGCAGTTTTTGGGt                                                              <  2:213193/251‑1 (MQ=255)                                                                                                                                                                             aaaaaCATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGCTGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCTTGTTGGCATTTTGAGGGTTAATcacac                                                     >  2:344598/1‑251 (MQ=255)                                                                                                                                                                             aaaaaCATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATcacac                                                     >  2:326466/1‑251 (MQ=255)                                                                                                                                                                                       tGATGCAACTCATTCAAGATCATGTCTCTCGCATTTAATAAATCGTAGCGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGttt                                           >  1:226194/1‑251 (MQ=255)                                                                                                                                                                                       tGATGCAACTCAGTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTAGGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAGCTCCAACCCTTTTCCCGGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGGTGGCATTTTGTGGGTTAATCACACCACACTGtt                                            <  1:415586/250‑1 (MQ=255)                                                                                                                                                                                         aTGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTtat                                         <  1:326466/251‑1 (MQ=255)                                                                                                                                                                                               cTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGt                                   >  2:25515/1‑251 (MQ=255)                                                                                                                                                                                                 cATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGGTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCAGCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCt                                 <  1:49961/251‑1 (MQ=255)                                                                                                                                                                                                      aaGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGTCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTAtttt                            >  1:242344/1‑251 (MQ=255)                                                                                                                                                                                                                  ctcGTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCAATCTCTTCGTGTTTTAATAATTCACATAAAAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAacac                   >  2:189489/1‑248 (MQ=255)                                                                                                                                                                                                                     gTATTTAATAAATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGAttt             <  1:33265/251‑1 (MQ=255)                                                                                                                                                                                                                            ataaATCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGACGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACAt        >  2:71693/1‑249 (MQ=255)                                                                                                                                                                                                                                aTCGTAGAGCACTTTATTAATTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTAt  <  1:47337/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        GATTTCCAGCCAGAACGACGACATTTTTATTAATACGGCTATCTTTATTACCCTCCATATTTCGCATAGAATTTAGCAAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTAT  >  NC_000913/3633426‑3633899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑