Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,018,847	G→A	Q121Q (CAG→CAA)	matP →	macrodomain Ter protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,018,847	0	G	A	100.0%	61.8 / NA	19	Q121Q (CAG→CAA)	matP	macrodomain Ter protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/14);  total (5/14)

GCCGTCGATGTGTTGCTCTCGCTGGAAAATGAACCCGTGCTGGTAAATGGCTGGATTGACAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCC  >  NC_000913/1018602‑1019082                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            gCCGTCGATGTGTTGCTCTCGCTGGAAAATGAACCCGTGCTGGTAAATGGCTGGATTGACAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATCTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGAt                                                                                                                                                                                                                                        <  1:230269/251‑1 (MQ=255)       gATGTGTTGCTCTCGCTGGAAAATTAACCCGTGCTGGTAAATGGCTGGATTGACAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGa                                                                                                                                                                                                                                  <  1:389193/251‑1 (MQ=255)                                                             aaGCATCTGAATTCGGAACTGGTCAATCGCATGAAGTAAACCATTCGGGCAAGACTAAAGCGTCAGTTTAATTCGAAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGATGGTCTTGCGCAGCGTCGCGGTAAAACGCGTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCATGATCt                                                                                                                                                                            <  2:338669/251‑1 (MQ=255)                                                                            gAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTggg                                                                                                                                                             <  1:404908/251‑1 (MQ=255)                                                                                    cAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAAt                                                                                                                                                     >  1:27783/1‑251 (MQ=255)                                                                                     aaTCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATa                                                                                                                                                    <  2:33317/251‑1 (MQ=255)                                                                                         gCATGAAGCCAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTg                                                                                                                                                >  2:415103/1‑251 (MQ=255)                                                                                                              aaaGACTAAAGCGCCATTTTAATGCAGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGGCTGGCAACGTCTGGCAGTTCTTGCGCAGCGTCGCGGTAAATCGCGTTCTGAAACGATTGTTCAAGTGATTGAAGATGCGGAAAACAAAGAGAATTACGCGAATAAAATTTCTTCTCTGAAGCAGGATCTGCAGGCATTGGTGGGTTAGGAATAACCGACGAAAGTCAGTTCAATTTAc                                                                                                                           <  1:140559/250‑1 (MQ=255)                                                                                                               aaGACGAAAGAGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACt                                                                                                                          >  2:316198/1‑251 (MQ=255)                                                                                                                                                         cAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCGCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTGCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAg                                                                                <  1:284902/251‑1 (MQ=255)                                                                                                                                                                 cgatcgatCGGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAAGGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGaa                                                                         <  1:427884/250‑1 (MQ=255)                                                                                                                                                                                                tCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGCTTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATATAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTg                                         >  2:330099/1‑251 (MQ=255)                                                                                                                                                                                                   ggctggTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGAt                                       <  1:223151/250‑1 (MQ=255)                                                                                                                                                                                                     ctggTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAcc                                    >  1:440906/1‑251 (MQ=255)                                                                                                                                                                                                               gcAGCGTCGTGGTATAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAACACGCGAATAAAATGGTTTCTCTGACGCAGGATCTGCAGGCATTGCTGGGTAAGGAATACCTGACTAAAGTCAGTTGAATTTATTAAAGGCAAAAAAACCCCCGCAGCATTGGGGTTTTTCTACCAGACTAGTACTTAAGCCTGGGGCGGAGTTACAACGTCTTTGATCCCTTTAACTTCg                          <  1:441814/251‑1 (MQ=255)                                                                                                                                                                                                                      cgcgGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATctct                    <  1:415103/250‑1 (MQ=255)                                                                                                                                                                                                                      cgcgGTAAAACGCTTTCTGAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTa                   <  2:176798/251‑1 (MQ=255)                                                                                                                                                                                                                                    ttCTGAAACGATTGTTCAACTGATTTAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGTTCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTGTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGTTCTCTACGcgaccattcgg      <  2:245938/250‑8 (MQ=255)                                                                                                                                                                                                                                        gAAACGATTGTTCAACTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATGCCTTTAACTTCGATCTCTACGCGACGATCCGGAGcc  <  1:380345/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            GCCGTCGATGTGTTGCTCTCGCTGGAAAATGAACCCGTGCTGGTAAATGGCTGGATTGACAAGCATATGAATCCGGAACTGGTCAATCGCATGAAGCAAACCATTCGGGCAAGACGAAAGCGCCATTTTAATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCC  >  NC_000913/1018602‑1019082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑