Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,228,095	IS2 (+) +5 bp	coding (17‑21/660 nt)	ycgM →	putative isomerase/hydrolase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1228095 =	0 (0.000)	24 (0.600)	24/492	0.5	100%	coding (17/660 nt)	ycgM	putative isomerase/hydrolase
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region
*	?	NC_000913	= 1228099	0 (0.000)	27 (0.670)	26/496	0.5	100%	coding (21/660 nt)	ycgM	putative isomerase/hydrolase
	?	NC_000913	= 1469240	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

CGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1227878‑1228099 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCA  <  NC_000913/1469240‑1469020                                                                                                                                                                                                                                                                                                                                                                                                                                                             CGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGAATCAACATCACAACTGGTAGACTGGCCCCTTGAATCTCCAGACAAC                                                                                                                                                                                                  >  1:133232/1‑251           AAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACT                                                                                                                                                                                        <  1:29076/251‑1           AAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAAAAGACGACACTAACAAATCACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCACCATCAAAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACT                                                                                                                                                                                        >  1:269406/1‑251                TAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAAT                                                                                                                                                                                   >  1:303319/1‑251                            AACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGTCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCATTTAAATAAGTGATAGTCT                                                                                                                                                                       <  2:193059/251‑1                                 AGCAAGGTTACTATTTGCAGTTACCGCCCCCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGTCACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATCAGGTGAGGGGAACGTTATGTATCCACATCACAACTGGTAGACTGGCCCCCTGATTATCAAGACCACCAATATACCTTAAATATGTGATAGTCTTAtt                                                                                                                                                                   >  2:105216/1‑248                                   CAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACT                                                                                                                                                                <  1:101658/251‑1                                                      TACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATT                                                                                                                                             >  1:324587/1‑251                                                              ccaaCCGAAGATTTGCTGCAGAAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTTTCAACATCACAACTGGTAGACTGGCCCCCCGAATCTCCAGACCACCAATAACCCTTAAATAAGTTATAGTCTTAATACCAGTTTTTAGACTAGTCATTTGAGAcc                                                                                                                                      >  2:228822/5‑248                                                                    GAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGAT                                                                                                                               >  1:383722/1‑251                                                                       GATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGA                                                                                                                            <  1:283211/251‑1                                                                                    AACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACTATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCG                                                                                                               <  1:24286/251‑1                                                                                      CATCTTTCCGTCATGGGGCAGAAAACAGACGACACTACCAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGCACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGG                                                                                                              >  1:429265/1‑250                                                                                         CTTTCCGTCATAGGGCAGAAAACAGAAGACACTAACAACTAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCAAGATAAGGTGAGGGGAACGTTATGTATCAACATCACAAATGGAAGACTGGCCACCTGAATCTCCAGACCACCAATATCAATTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGACGGAGA                                                                                                           >  2:389430/1‑250                                                                                                  ATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACG                                                                                                 >  1:48206/1‑251                                                                                                              ACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGACTCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACc                                                                                     >  1:188894/1‑250                                                                                                                  ACGACACTAACAAATAACCGATATCCGGCGGAGGCATTATCTTTGTCGGCGCGGGCTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACCACTGGTAGACTCGCCCCCTGAATCTCCAGACAACCAATATCACTTACATACGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGA                                                                                  >  1:413490/1‑250                                                                                                                       ACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGCTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGAAAACAAATATCACTTAAATACGTGATAGTCTTAATACTAGATTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAA                                                                                >  1:404776/1‑247                                                                                                                        CTAACAAATAACCGATATCCGGCGGGGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTATTTTTTAGACTATACATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGTAAA                                                                                >  2:134265/1‑246                                                                                                                              AATAACCGATATCAGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAATGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGTAGAACAGATGATTGTTGTCTTAGGGCCGGAGAAACGACGACGGCGTACCACACAGGAAAAGATCGCAATT                                                                     >  2:145157/1‑251                                                                                                                                          CCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGGGAGGGGAACGTTATGTATCAACATCACAACTGGGAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGAATAGTCATTGGAGTACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGC                                                         >  2:307407/1‑251                                                                                                                                                                      GGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACATGAAAAGATCGCAATTGTTCAGCAGAGCTTT                                                      <  2:418475/226‑1                                                                                                                                                                      GGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTT                                                      >  1:418475/1‑226                                                                                                                                                                       GGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAAACGGGGATGACGGTCTCCCTCGT                            >  1:320471/1‑251                                                                                                                                                                             CATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGTGGATGACGGTCTCCCTCGTTGCCCG                      >  2:324822/1‑251                                                                                                                                                                                   CACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAAC                 >  1:114030/1‑250                                                                                                                                                                                                 GGGAACGTTATGTATCAACATCACAACTGGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAGCCGGGGATGACGGTCTCCCTCGTTTCCCGGCAACATGGTGTAGCAGCCA  <  2:383722/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                             CGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAGAAAACAGACGACACTAACAAATAACCGATATCCGGCGGTGGCATTATCTTTGTCGGCGCGGGTTTTCATATCCACGATAAGGTGAGGGGAACGTTATGTATCAACATCACAACTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1227878‑1228099 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCA  <  NC_000913/1469240‑1469020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.