Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,036,781 G→A F442F (TTC→TTT)  recJ ← ssDNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,036,7810GA97.8% 129.9 / ‑3.5 46F442F (TTC→TTT) recJssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (31/14);  total (32/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

CGGTTCGACCATCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAA‑CAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAG‑CATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGAT  >  NC_000913/3036538‑3037025
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    
cGGTTCGACCCTCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGACAAA‑CAGCGGCTCCGGGAACATCTGCCAACACGGGCCAG‑CATCGCGCAGCCGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCCCTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCCCTAAGTAACCAATTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTAt                                                                                                                                                                                                                                               >  2:163273/1‑251 (MQ=255)
            cACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAA‑CAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAG‑CATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTACAGCg                                                                                                                                                                                                                                   >  1:267865/1‑251 (MQ=255)
              ccTTCAAATGACGTTCGCCCACCAGCCGCTGTTCCAGCAGACGGAAATGACCGTCAAA‑CAGCGGCTCCGGAAACATCTGCCCCCACGGGCCAG‑CATCGAGCAGCAGCTGCGCCACTTCCATGGTCATTTAGACCGCGCTTAACGGACCGTC‑TGATACAACTTCGCC‑TTGCAATCGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGACAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGAc                                                                                                                                                                                                                                 >  1:63518/1‑251 (MQ=255)
                ttCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAA‑CAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAG‑CATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACa                                                                                                                                                                                                                                >  1:268658/1‑250 (MQ=255)
                                    caGCCGCTGTTGCAGCAGATGGAAATGACCG‑CAAAGCAGCGGTACCGGAAACTCCTGCCCCC‑CGGGGCAGGCATCCCCCAGCAGCTGCCCCACTTCCAGGGTCTTTCCGGCCGGGTTTAACGGACCGTC‑GGACACCCCTCCGCC‑GTGCAAAAGC‑AAAGGGTCCAGCCACTCAGTAACCATTTCGCCAAACCGTTGTTGAAAGATTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGAc                                                                                                                                                                                                           <  2:244379/251‑1 (MQ=255)
                                          cTGTGGCAGCAGACGGAAATGACCGTCAAA‑CGGCGCCTCCGGTAACATCTGCCCCCGCGGGCCAG‑CATCGCGCAGCGGTTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCCCC‑TTGCAATAGC‑GAAGGTTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCGCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGcc                                                                                                                                                                                                       <  1:387930/249‑1 (MQ=255)
                                                  gcagACGGAAATGACCGTCAAA‑CAGCGGCTACGGGAACATCTGCCCCCACGGGCCAG‑CATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGATTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGc                                                                                                                                                                                             >  1:68722/1‑251 (MQ=255)
                                                   cagACGGAAATGACCGTCAAA‑CAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAG‑CATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGc                                                                                                                                                                                             >  1:74104/1‑250 (MQ=255)
                                                                     aaa‑CAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAG‑CATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATg                                                                                                                                                                                                             >  1:309259/1‑216 (MQ=255)
                                                                             ggCTCCGGGAACATCTGCCCCCACGGGCCAG‑CATCGCGCAGCAGCTGCGACACTTCCCTGGTCATTTCGGCCGGGCTTACCGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCCTCATGCCCGGGTAGGgtgtggctc                                                                                                                                                                    >  2:15670/1‑246 (MQ=255)
                                                                                  cGGGAACATCTGCCCCCACGGGCCAG‑CATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCt                                                                                                                                                              >  2:127482/1‑251 (MQ=255)
                                                                                    ggAACATCTGCCCCCACGGGCCAG‑CATCGCGCAGCAGCCGCGACACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGCCCGCCAAACTTCAGCATCATGCCAGGGAAGAGTGTGTCTAATCGCTcc                                                                                                                                                            >  1:426355/1‑251 (MQ=255)
                                                                                                 ccACGGGCCAG‑CATCGCGCAGCCGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCA‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGCAGATCGTGTCTAATCGCTCCAGCGGATCACGc                                                                                                                                                >  2:12951/1‑250 (MQ=255)
                                                                                                             cATCGCGCAGCAGATGAGCCCCTTAAACGGTCATTAAGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCAAGCCAATCCGTAACCAGTTCGCCAAACAGTTGTTGAAAGAGTTTACATTTATCCTCTTCCAGCGACAAACCCGCCGCCATAGCATGACCGCCAAACTTCCGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGcccc                                                                                                                                    >  2:452306/1‑251 (MQ=255)
                                                                                                                    cagcagCTGCGCCCCTCCCATGTTCATTCCGGCCGGGCTTACCGGCCCGTC‑TGATCCCCCTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCAGAATgg                                                                                                                             <  1:127482/251‑1 (MQ=255)
                                                                                                                             cgcCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATCCCCCTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCACCAAACCGTTGTTGACAAAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCgg                                                                                                                    >  1:423581/1‑251 (MQ=255)
                                                                                                                               cctcttctgtGGTCATTTCGCCTGGGATTAACGGTCGGTC‑TCATCCCTCTCCGCC‑TTGCAATAGC‑GAAGGTCCCAACCACTATTTAACCAGTTCGCCAAACCGTGTTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCAGCACGCATATGCAGCCCCTGAATGGAGCGACCGGaa                                                                                                                  <  2:315521/242‑1 (MQ=255)
                                                                                                                               cccctcccATGGTCATTTCGCCCGGGTTTAACGAACCTTG‑TGATGCCA‑TCCGCCCTTCCAATGGC‑GAAGGGTCCAGCCACTCAGTAACCATTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTACCCTCTCCCGGCATCAAACCCGCCGCCATGGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGaa                                                                                                                  <  1:201444/245‑1 (MQ=255)
                                                                                                                                                     cGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCCGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctg                                                                                            >  2:318118/1‑251 (MQ=255)
                                                                                                                                                       ggCTTAACGGACAGTC‑TGATACCACTTCACC‑TTGCAATCAC‑GAAGGGTCCAGCCACACAGAAACCAGTTCGCCAAACCGATGTTGAAAGAGATTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCACCAAACTTCAGCATCATGCAACGGGAGAGTGTGTCTAATCACTCCAGCGCATCACGCATATGCAGCCCCTGACTGGAGCGACCGGAACCTTTCATCGCACCGTCCCcttct                                                                                          >  2:325627/1‑248 (MQ=255)
                                                                                                                                                        gtttgaacgACCGTCATGATCCCCCTCCGCC‑TTG‑AATAGCAGAGGGGCCCCGCCACTCAGTACCCAGTCCGCAAACCCGTTGGTGAAAGATTTTAAATTTATCCCCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgct                                                                                          <  2:72766/243‑1 (MQ=255)
                                                                                                                                                          ttAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGgc                                                                                       >  2:200986/1‑251 (MQ=255)
                                                                                                                                                                    gTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAACTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGCCCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                                                                              >  1:262056/1‑250 (MQ=255)
                                                                                                                                                                    gTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCCGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                                                                              >  2:380595/1‑250 (MQ=255)
                                                                                                                                                                     cc‑TGATCCCACTTCGCC‑TTGCAAAAGC‑GAAGGCCCCAGCCACTCATTACCCATCTCCCCAAACCGTTGTTGAAAGAGTTAAAATTTATCCTCTTCCAGCAACAAGCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCGGGCGCAAGCGCGAt                                                                            <  1:366388/250‑1 (MQ=255)
                                                                                                                                                                                         tgaaaTAGG‑GAGGGGTACAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTGGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaaa                                                            <  1:8627/246‑1 (MQ=255)
                                                                                                                                                                                          gCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGc                                                         >  1:294606/1‑251 (MQ=255)
                                                                                                                                                                                               aggagAAGGTTCCATCCACTCATTAACCAGTTCGCCAAACCGTTGTTGAAAGATTTTAAATTTATCCTCTTCCAGCGACAACCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCtt                                                     <  1:296876/247‑1 (MQ=255)
                                                                                                                                                                                                gC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCACACTTCAGCATCATGCCAGGGAAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTAAGCGTACCGTCACCTGCTGGCGCAAACGCGATCACCGGACGGTGAAAACGCTATTTg                                                   >  1:324657/1‑251 (MQ=255)
                                                                                                                                                                                                   gAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCAAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGAt                                                 >  1:435801/1‑251 (MQ=255)
                                                                                                                                                                                                    taGGGTCCATCCACTCAGTAAACCGTTCGCCAAACCGTTGTTGAAAGCGATTAAATTAATCCACTTCCAGCGACAAACCAGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGAAGAGTGTGTCTAATCGCTCCAACGAATCACACATATGCAGCCCCTGAATGGGGCGACCGGCACCTTTCAGCGTACCGTCACCTGCTGGAGCAAACGCGATACCCGAACGGCGAACACGCTCTTTgctg                                                >  2:340032/2‑248 (MQ=255)
                                                                                                                                                                                                           cAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGa                                           >  1:391687/1‑249 (MQ=255)
                                                                                                                                                                                                               cacgcAGTACCCAGTTCGCCAAACCGTTGTTGAAAGGGTTTAAATTTATCCCCTTCCAGCGACAAGCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg                                     <  1:208049/247‑1 (MQ=255)
                                                                                                                                                                                                                   cAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGaa                                   <  1:223785/249‑1 (MQ=255)
                                                                                                                                                                                                                      tAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCg                              >  1:311473/1‑251 (MQ=255)
                                                                                                                                                                                                                           aGTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAAc                         >  1:311451/1‑251 (MQ=255)
                                                                                                                                                                                                                           aGTTCGACAAACCGTTGTTGAAAGAGTTTACATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCACACTTCAGCATCATGCCAGGGTAGCGTGTGTATAATCGCTCCAGCGCATCACGCATATGCAGCCACTGAATGGCGCGACCGGAACCTTTCAGCGTCCAGTCACCAGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTCTGATGCGCGAAGCCAGCATACTGACAAc                         >  2:412748/1‑251 (MQ=255)
                                                                                                                                                                                                                            gTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTACCCCCTTACAGCGACACACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGAAGAGTGTGTCTAATCGCTCCAGCGCATAACGCATATGCAGCCCCTGAATGCAGCGACCGGAACCTTTCAGCGTACCGTCCCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACg                        >  1:56579/1‑251 (MQ=255)
                                                                                                                                                                                                                            gTTCGCCAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGCATACCGACAACg                        >  2:352713/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ccAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAACCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCa                                                                                                           <  1:341105/163‑1 (MQ=255)
                                                                                                                                                                                                                                 ccAAACCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCa                                                                                                           >  2:341105/1‑163 (MQ=255)
                                                                                                                                                                                                                                    aaCCGTTGTTGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATg                >  1:250317/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gttgttGAAAGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCAt            <  1:230613/251‑1 (MQ=255)
                                                                                                                                                                                                                                              gAAAGAGTTTAAATTTATCCTCTCCCAGCGACAACCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCgggg      <  1:29349/251‑1 (MQ=255)
                                                                                                                                                                                                                                               aaaGAGTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTCATGCCATTCGGGGt     >  1:50572/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  gagTTTAAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGAt  >  1:340970/1‑251 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    
CGGTTCGACCATCACCTTCAAATGACGTTCGCCCACCAGCCGCTGTTGCAGCAGACGGAAATGACCGTCAAA‑CAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAG‑CATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTC‑TGATACCACTTCGCC‑TTGCAATAGC‑GAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGAT  >  NC_000913/3036538‑3037025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑