Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,244,460	G→C	H94Q (CAC→CAG)	uxaC ←	D‑glucoronate/D‑galacturonate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,244,460	0	G	C	100.0%	141.9 / NA	42	H94Q (CAC→CAG)	uxaC	D‑glucoronate/D‑galacturonate isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/26);  total (16/26)

TCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGT  >  NC_000913/3244224‑3244705                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      gcctgtgCGAGCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGTTGCCGACCAGTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACTGACGGCGCAGTTCGAGGGGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGTAGAACAGta                                                                                                                                                                                                                                           <  2:262896/243‑2 (MQ=255)        cGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGccc                                                                                                                                                                                                                                    >  1:364790/1‑249 (MQ=255)                       gCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTGCGAGGTGCGGCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTc                                                                                                                                                                                                                    <  2:218704/250‑1 (MQ=255)                             gagaATCGATCGGGTCATCGGTGGTGCCGAACATTTTCACGTGCAACTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGGTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGAGAAACCAAACGGACGGCGCAGCACGAGGGGGGTCCAGTGGTATAACGGGGTGCCGAGAGTCAGCGGAGCAGTCGCCGCCCAGGGGGCAACTTTTTCACGGTc                                                                                                                                                                                                              >  1:297032/1‑250 (MQ=255)                               gaATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTc                                                                                                                                                                                                              >  1:319854/1‑248 (MQ=255)                                actcgatcgGGTCATCGGTGGAGCCGACCATTTTCAAGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGAGCGTCCAGTGGTATCACGGGTTGCCGATAGGCTGCGGAACAGTCGCCGCCCAGGCGGCCAATTTTTACCGGTCAGAc                                                                                                                                                                                                          >  2:249449/3‑251 (MQ=255)                                     atcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATc                                                                                                                                                                                                     <  1:339785/251‑1 (MQ=255)                                       cgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGGCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCa                                                                                                                                                                                                    <  1:216530/250‑1 (MQ=255)                                       cgGGTCATCGGTGGTGCCCACCCTTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCTAGGCGTCAAATGTTTCACGGTCATACGCATCa                                                                                                                                                                                                    <  2:434056/250‑1 (MQ=255)                                                   ggtgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGAc                                                                                                                                                                                       >  1:260341/1‑251 (MQ=255)                                                       ccgtccATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTGCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGGATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCATGCGGCAAATTTTGCACGGGCAGACGCATCACCGGTACACAGACGCTc                                                                                                                                                                                   <  2:445134/247‑1 (MQ=255)                                                        cGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCGGCGCCAGCAGTTCGTTACATTCGTTCCCGATTTCATCGGCAGTTGACGGAGAAAGCAAGTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACCGCCGCTca                                                                                                                                                                                  <  2:234268/251‑2 (MQ=255)                                                          aCCATTTTCTCGTTCATCTGCTTCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGc                                                                                                                                                                                <  1:237698/251‑1 (MQ=255)                                                          aCCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGc                                                                                                                                                                                <  1:186682/251‑1 (MQ=255)                                                                    cGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCGGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGGCAGACGCAGCACCGGTACACAGACGCTCGGCCACACCGTTg                                                                                                                                                                      <  1:156997/251‑1 (MQ=255)                                                                                       gCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACATTCGCCGCCCAGTCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCAtt                                                                                                                                                   <  1:269922/251‑1 (MQ=255)                                                                                           cgcgcgGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGt                                                                                                                                                >  2:296679/1‑250 (MQ=255)                                                                                              gcgGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGGCGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTg                                                                                                                                            <  2:242869/251‑1 (MQ=255)                                                                                                 gagaAGTTATTCTGCGCCAGCAGGTCGTTACATTCGTTCCAGATTTCAGCGGCAGTTGACGGAGAAATCAATTTGCCAGTGATACCAAGCGGCCGGCGCAGTTCGGTGTGCGTCCAGTGGTATAACGGGGTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTCCACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTATTGATc                                                                                                                                         <  2:22135/251‑1 (MQ=255)                                                                                                 gagaAGTTATCAGTCGCCAGCAGTTCGTTACATTCGTTCCAGATGTCATCGGCAGTTGAAGGAGAAAGCAAGTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCTCCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCATGCGTCAAATGTTTCACGGTCAGACGCATCACCGGTACATAGACGCTCTGCTACAACGTTGGTACGCAGAgcgcgaaagtggtattgatc                                                                                                                                         <  2:64391/251‑16 (MQ=255)                                                                                                       ttATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGAAGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCttt                                                                                                                                   >  1:435729/1‑251 (MQ=255)                                                                                                         aTCCTGCGCCAGCAGTTCGTTACATACGTTACAGATTTAATCAGCAGTTGCCGGAGAAAGCAATTTAACAGTGATACCAAAAGGGCGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGACCAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACCCAGACGCTCGGTCACACAGTTGGTAAGCATAACGCGCCATTTGTAGAGATCCCCTTTc                                                                                                                                  >  2:293355/1‑250 (MQ=255)                                                                                                               cgcCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACCGCGCAAGCAATTTGCCAGTGATACCCAACGGCCGGCGCAGTTCGCGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCCCCGGTACACAGACGCCAGGCCACACCGTTGGTACGCATAGCGCGCCCTTTTTAGTGAACGCCTTTCAGCCAgc                                                                                                                           >  2:324303/1‑250 (MQ=255)                                                                                                                   agcagTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTc                                                                                                                       <  1:250626/251‑1 (MQ=255)                                                                                                                   agcagTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGATCGAGGTGCGAACAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCCAGACCTTTGTAGTGATCGCCTTTCAGCCAGATGTc                                                                                                                       >  1:268566/1‑251 (MQ=255)                                                                                                                    gcagTTCGTTACATGCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCa                                                                                                                      <  1:390207/251‑1 (MQ=255)                                                                                                                           gTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGt                                                                                                               <  2:315469/251‑1 (MQ=255)                                                                                                                              aCATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGGGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtttt                                                                                                            <  2:149365/251‑1 (MQ=255)                                                                                                                                 ttCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCCCACCGTTGGTACGCATAGCGCGCCATTTGTAGTGCTCGCCTTTCAGCCCGATGTCCTACAGGtttttt                                                                                                          >  2:306140/1‑249 (MQ=255)                                                                                                                                        cAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATa                                                                                                  <  1:278035/251‑1 (MQ=255)                                                                                                                                               cATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGAGACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTc                                                                                            <  1:386703/250‑1 (MQ=255)                                                                                                                                                 gcGGCAGGTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGCTAGTCTGCTGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCTACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCAGCCAGGTTTTTAAAACGTTGGTCTTCCGc                                                                                         <  2:260341/250‑1 (MQ=255)                                                                                                                                                        ttGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATctgc                                                                                  >  1:49529/1‑251 (MQ=255)                                                                                                                                                                      aaTTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGCATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCAAACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCaactg                                                                    >  2:32015/1‑248 (MQ=255)                                                                                                                                                                                         acggacggCGCAGTTCGAGGTGAGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGCTGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGTCAATGGTAATCGAAAATc                                                 >  2:85675/1‑251 (MQ=255)                                                                                                                                                                                             acggCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCt                                             >  2:212345/1‑251 (MQ=255)                                                                                                                                                                                                   cAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTGTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCtt                                       <  1:253452/251‑1 (MQ=255)                                                                                                                                                                                                    aGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGAGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCAGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCttt                                      >  1:373384/1‑251 (MQ=255)                                                                                                                                                                                                       tCGAGGTTCGTCCAGTGGTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTg                                    <  1:131274/250‑1 (MQ=255)                                                                                                                                                                                                                        gTATAACGGGTTGCCGATAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACGGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTGTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGAc                  <  2:352055/251‑1 (MQ=255)                                                                                                                                                                                                                              cGGGTTGCCGATAGTCGGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTATTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCggg            <  2:335944/251‑1 (MQ=255)                                                                                                                                                                                                                                        aTAGTCTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCTAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGt  <  1:64983/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      TCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGT  >  NC_000913/3244224‑3244705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑