Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,470	C→T	P471S (CCG→TCG)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,470	0	C	T	100.0%	93.0 / NA	28	P471S (CCG→TCG)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/3);  total (25/3)

CTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTC  >  NC_000913/3017224‑3017705                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            cggttgAAACCTTGCGGCTAGCTGCGCTTAAGTGCCTGCCTGCGCGTGCTGGAAGGCTCAGACGCCTGGGGACTTTACCATGTGGACGTCGAACGACGCAACAGACTGGAAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATGGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTTCGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGa                                                                                                                                                                                                                                         <  2:318148/247‑1 (MQ=255)     tgAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATAc                                                                                                                                                                                                                                     >  1:451919/1‑251 (MQ=255)       aaaCCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATaact                                                                                                                                                                                                                                   >  1:254197/1‑248 (MQ=255)                   tATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGCAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACCTCCGTCTGCTTgg                                                                                                                                                                                                                       >  2:187553/1‑251 (MQ=255)                    aTCTGCGCTTAAGTGACTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGCAGCCAGAAGAGCGTATTGAAGTGGCAGAAGCCCTGCCGCTGCCCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGAAAGATATTTCGGAATACATCCGTCTGCTTGGc                                                                                                                                                                                                                      >  2:408023/1‑251 (MQ=255)                           cTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACAcc                                                                                                                                                                                                               >  1:374349/1‑251 (MQ=255)                                gTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTAt                                                                                                                                                                                                          >  1:266371/1‑251 (MQ=255)                                  gCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGGGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGc                                                                                                                                                                                                        >  1:40024/1‑251 (MQ=255)                                     tGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGGTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGGGGCAGAAGCCCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATGTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCg                                                                                                                                                                                                      <  2:294689/250‑1 (MQ=255)                                            gcgAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTg                                                                                                                                                                                              <  1:361283/251‑1 (MQ=255)                                               aGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATCTCGGAATACATCCGTATGCTTGGCGAACACCGCTATGCCGACGCGCTGGa                                                                                                                                                                                            >  2:99695/1‑250 (MQ=255)                                                 cTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACt                                                                                                                                                                                         >  1:397656/1‑251 (MQ=255)                                                          tCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTAcc                                                                                                                                                                                 >  1:387547/1‑250 (MQ=255)                                                                                  gTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAc                                                                                                                                                        >  1:222785/1‑251 (MQ=255)                                                                                          cGAACGACTGAACAGACTGGCAGCAGATGCGTTAACAATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCAGCCATTACCGGTCat                                                                                                                                                 >  1:143528/1‑250 (MQ=255)                                                                                                  tGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGACGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCACGATATTTCGGAATACCTCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCACCGCTCTGCCCGCCATTACCGGTCATAttttcgct                                                                                                                                        >  2:162914/1‑246 (MQ=255)                                                                                                                           aaCCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTAccc                                                                                                               >  1:379576/1‑251 (MQ=255)                                                                                                                                          ccAGAATCACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGAc                                                                                                 >  1:128211/1‑250 (MQ=255)                                                                                                                                             gAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGAATGCTACGTTGACCCCTGTGTTCCGGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCAGACGCGCTGGAACTCATCTCCCAACGCAACGCTCGGCCCGCCATTACCAGTCATATTTGCGATCACCAGTGCCCATACCACTGTACCCGCCTGGATTCCGACAGTg                                                                                             >  2:155181/1‑251 (MQ=255)                                                                                                                                                       aaGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTgc                                                                                            >  1:204190/1‑242 (MQ=255)                                                                                                                                                          ccAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACAGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCcg                                                                                >  1:381693/1‑251 (MQ=255)                                                                                                                                                             gaagaGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCcgcg                                                                              >  1:440475/1‑250 (MQ=255)                                                                                                                                                                                cAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGACCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTg                                                          >  1:182920/1‑251 (MQ=255)                                                                                                                                                                                              cTGACCGACTGCTACGTTGCACCCTGTGTTACTGCCTGCGCTATCAAGCAAGCTATTTCGGAATACATACGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCACAGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTAGCGCTGGAAAAAGGTTggg                                             >  2:168841/1‑250 (MQ=255)                                                                                                                                                                                                    gACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAat                                       >  1:397982/1‑250 (MQ=255)                                                                                                                                                                                                                         gtTACTGCCTGCGCTATCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACCCCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGAAACGCTGGCACAAAcc                 >  2:183148/1‑251 (MQ=255)                                                                                                                                                                                                                           tACTGCCTGCGCTATCAAGCAAGATATTTCGGTATACATCCGTCTGCTCGGCGCACACCGCTATGCCGCCGCGCTGGAACTCATCTACCAACGCAAAGCTCTGCCCGCCATTACCGGTCATATTTGCGATCAACAGCACCAATACAACTGTACCCGCCTGGAATACGACAGTGCGCTGAATATCCGCGAACTGAACAAAGTCGCGCTGGAAAAAGGTTGGGATGCATATAAGCAACGCTGGCACAAAcc                 >  1:454630/1‑249 (MQ=255)                                                                                                                                                                                                                                         tCAAGCAAGATATTTCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCCATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAACAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGATCTGGTTc  >  1:305582/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            CTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTC  >  NC_000913/3017224‑3017705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑