Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,039,555	A→G	L26L (TTA→TTG)	msrP →	protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,039,555	0	A	G	100.0%	42.7 / NA	18	L26L (TTA→TTG)	msrP	protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/7);  total (11/7)

AATATGGGTATTCAACCTATCGTGGCAGTTAATGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTAAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATG  >  NC_000913/2039338‑2039787                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         aaTATGGGTATTCAACATATCGTGGCAGTTAATGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCActttct                                                                                                                                                                                                         <  2:336269/251‑1 (MQ=255)       ggTATTCAACCTATCGTGGCAGTTAATGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACCCTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCt                                                                                                                                                                                                   <  1:100822/251‑1 (MQ=255)       ggTATTCAACCTATCGTGGCAGTTAATGCATAAATATGTAAAAGAAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCCGGCTAACGACCAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCt                                                                                                                                                                                                   >  1:451582/1‑251 (MQ=255)                        ggCAGTTAATGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAAGCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCg                                                                                                                                                                                   <  2:207614/250‑1 (MQ=255)                                    aTAAATATGTAAACGCAGATCTCGGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTCACGACAAAAAAGAGTGATGGCTTATGAAAACGAATCAATTTTTAAAAGACTCAGATGTTACGGCCGAGACGGTATTCTTTATGAAGCGTCGGCAGGGGTTGAAAACACTGGGCAACATAGCAAATGCAGTTTCTGGGTCTCACGCTggacagtgcgatcaccttagctg                                                                                                                                                                      >  2:229880/1‑229 (MQ=255)                                       aaTATGTAAAAGCAGATTTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTATCTGGtt                                                                                                                                                                   <  1:401256/251‑1 (MQ=255)                                        atatGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGttt                                                                                                                                                                  <  1:129416/251‑1 (MQ=255)                                         tatGTAAAAGCAGATCTCTGCTACTGTTAGGGCGGACACTCCCTGATAGATTAACAGTAAACGGCTCTTGCTGGCTAACGCCAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGACTCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCTGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGttt                                                                                                                                                                  >  1:424375/1‑250 (MQ=255)                                                     gATCTCTGCTACTGTTAGGGGGGACACTCCCCGACAGATTTACAGAAAACGGCTCTTGCTGGCTAACGACAACAAAGTGTGCTGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAATGCACTGGGCATCAGTGCAACTGCACTTTCTTTGACTCACGCTGCGCCTGCCACTCTGCTTAGCTGGTTTAAAGGGaccgttc                                                                                                                                                     >  2:88861/1‑245 (MQ=255)                                                                                                                             aaaaGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTAAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTg                                                                              >  1:327256/1‑250 (MQ=255)                                                                                                                                             aTGAAAAAGAATCAATTTTTAAAAGAATCAGCTGTTACGGCCGCGTCGGCATTCTTTATGACGCGTCGGCAGGTGTTGTAAGCACTGGGAAAAAGTGCAACTGCACTTTCTTTGACTAAAGGCGCGCATGCCGATCTGCATAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGGAAAGCGCTGGCGTTCGGCAAACCAGCCGCCTGGCACAATAACCTGCCACTGACGCCAGCAGAGAAAgg                                                             >  2:183659/1‑250 (MQ=255)                                                                                                                                                 aaaaGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGACGGTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTcc                                                         >  1:239630/1‑251 (MQ=255)                                                                                                                                                    aGAATCACTTTTTAACAGAATCAGATGTTACAGCCCCGCCGGTATTATTTAACAACCGTCGGCAGGTGTTGAAAGCACTGGGCCTCCAAGCACCTGCACTTTCTTTGCCTCACGCTGCGCATGACGCTCTACTTACCTGGTTTAACGGGAAACATCGCCCGCCCGCCCACGACGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAACTACCCTGCCACTGACGCCAGCAGAAAAAGtatcaggt                                                      >  1:402046/1‑244 (MQ=255)                                                                                                                                                         cAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTCTGAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCCCTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGAAAAAGTTTCCGGTtata                                                  >  1:131615/1‑250 (MQ=255)                                                                                                                                                                                tACGGCCGTGTCGTTATTCTTTATGAAGCGTCGGCAGGTGTTGAAAGCACTGGGAATCAGTGCAACTGCATTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTCTATGAATTCGGGCTg                           <  1:158769/250‑1 (MQ=255)                                                                                                                                                                                         tgcGGTATTCTTAATGAAGCGCCGGCAGGTGTTGAAAGCGCTGGGCATCAGGTCAACTGCTCTTTTTTTGCCTCCCGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGTGCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGCCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGTTTATAATAACTTCTATGAATTCTGGCTGGATAAAGcc                  <  1:144224/248‑1 (MQ=255)                                                                                                                                                                                         gTCGGTATACTTAAAGAAGCATCGGCAGGTGTTGCAAGCACTGGACATCAGAGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATATGCTTAGCTGGCTTAAAGGGAACGATCGACCGCCCGCCACCGCCGGAAAAGAGCGGGAGTTCAGCAAGCCAGCCGCCTGGCAAAATAACCTGCAACTGACGCCAGCAGATAAAGCTTCCGGTTATAATAACTTATACGAATTCGGGcgggctcaaccc                  >  2:388638/1‑239 (MQ=255)                                                                                                                                                                                                        gAAGCGTCGGCAGGTGTTGAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATg  >  2:152868/1‑251 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         AATATGGGTATTCAACCTATCGTGGCAGTTAATGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCTGGCTAACGACAAAAAAGTGTGATGGCTTATGAAAAAGAATCAATTTTTAAAAGAATCAGATGTTACGGCCGAGTCGGTATTCTTTATGAAGCGTCGGCAGGTGTTAAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTCTATGAATTCGGGCTGGATAAAGCCGATCCCGCCGCTAATG  >  NC_000913/2039338‑2039787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑